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Literature summary extracted from

  • Jiao, H.; Yin, Y.; Liu, Z.
    Structures of the mitochondrial CDP-DAG synthase Tam41 suggest a potential lipid substrate pathway from membrane to the active site (2019), Structure, 27, 1258-1269 .
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.7.7.41 expressed in Escherichia coli BL21(DE3) cells Schizosaccharomyces pombe

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
2.7.7.41 hanging drop vapor diffusion method, apoenzyme using 15% (w/v) Tacsimate, pH 7.0, 20 mM CdCl2. CTP-bound enzyme using 0.1 M HEPES-Na, pH 7.5, 9% (w/v) tacsimate, pH 7.0 and 2% (w/v) PEG3350 Schizosaccharomyces pombe

Protein Variants

EC Number Protein Variants Comment Organism
2.7.7.41 F240A the mutant retains about 47% of wild type activity Schizosaccharomyces pombe
2.7.7.41 K156A the mutant retains 6.81% of wild type activity Schizosaccharomyces pombe
2.7.7.41 K183A the mutant enzyme is almost inactive Schizosaccharomyces pombe
2.7.7.41 Q254A the mutant enzyme is almost inactive Schizosaccharomyces pombe
2.7.7.41 R180A the mutant enzyme is almost inactive Schizosaccharomyces pombe
2.7.7.41 R245A the mutant retains 29.89% of wild type activity Schizosaccharomyces pombe
2.7.7.41 Y144A the mutant enzyme is almost inactive Schizosaccharomyces pombe

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.7.7.41 EDTA the enzyme activity is almost completely inhibited when EDTA is added to the reaction Schizosaccharomyces pombe

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.7.7.41 0.094
-
 phosphatidate at pH 7.5 and 30°C Schizosaccharomyces pombe
2.7.7.41 0.26
-
 CTP at pH 7.5 and 30°C Schizosaccharomyces pombe

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.7.7.41 mitochondrion
-
 Schizosaccharomyces pombe 5739
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.7.7.41 Mg2+ highest activity with Mg2+, 2 mM used in assay conditions Schizosaccharomyces pombe

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.7.7.41 CTP + phosphatidate Schizosaccharomyces pombe
-
 diphosphate + CDP-diacylglycerol
-
 r
2.7.7.41 CTP + phosphatidate Schizosaccharomyces pombe 972
-
 diphosphate + CDP-diacylglycerol
-
 r
2.7.7.41 diphosphate + CDP-diacylglycerol Schizosaccharomyces pombe
-
 CTP + phosphatidate
-
 r
2.7.7.41 diphosphate + CDP-diacylglycerol Schizosaccharomyces pombe 972
-
 CTP + phosphatidate
-
 r

Organism

EC Number Organism UniProt Comment Textmining
2.7.7.41 Schizosaccharomyces pombe O74339
-
-
2.7.7.41 Schizosaccharomyces pombe 972 O74339
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.7.7.41 Ni-NTA column chromatography and Superdex 200 gel filtration Schizosaccharomyces pombe

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.7.41 CTP + 1,2-dioleoyl-sn-glycero-3-phosphate
-
 Schizosaccharomyces pombe diphosphate + CDP-1,2-dioleoylglycerol
-
 ?
2.7.7.41 CTP + 1,2-dioleoyl-sn-glycero-3-phosphate
-
 Schizosaccharomyces pombe 972 diphosphate + CDP-1,2-dioleoylglycerol
-
 ?
2.7.7.41 CTP + phosphatidate
-
 Schizosaccharomyces pombe diphosphate + CDP-diacylglycerol
-
 r
2.7.7.41 CTP + phosphatidate
-
 Schizosaccharomyces pombe 972 diphosphate + CDP-diacylglycerol
-
 r
2.7.7.41 diphosphate + CDP-diacylglycerol
-
 Schizosaccharomyces pombe CTP + phosphatidate
-
 r
2.7.7.41 diphosphate + CDP-diacylglycerol
-
 Schizosaccharomyces pombe 972 CTP + phosphatidate
-
 r
2.7.7.41 additional information the enzyme does not bind phosphatidylserine, phosphatidylethanolamine, or phosphatidylcholine Schizosaccharomyces pombe ?
-
-
2.7.7.41 additional information the enzyme does not bind phosphatidylserine, phosphatidylethanolamine, or phosphatidylcholine Schizosaccharomyces pombe 972 ?
-
-

Synonyms

EC Number Synonyms Comment Organism
2.7.7.41 CDP-DAG synthase
-
 Schizosaccharomyces pombe
2.7.7.41 CDS
-
 Schizosaccharomyces pombe
2.7.7.41 Tam41
-
 Schizosaccharomyces pombe

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.7.7.41 7.5
-
-
 Schizosaccharomyces pombe