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Literature summary extracted from

  • Meza, A.; Cambui, C.; Moreno, A.; Fessel, M.; Balan, A.
    Mycobacterium tuberculosis CysA2 is a dual sulfurtransferase with activity against thiosulfate and 3-mercaptopyruvate and interacts with mammalian cells (2019), Sci. Rep., 9, 16791 .
    View publication on PubMedView publication on EuropePMC

Application

EC Number Application Comment Organism
2.8.1.1 medicine CysA2 interacts with pulmonary cells, and it is capable to activate macrophages and dendritic cells Mycobacterium tuberculosis
2.8.1.2 medicine CysA2 interacts with pulmonary cells, and it is capable to activate macrophages and dendritic cells Mycobacterium tuberculosis

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
2.8.1.1 structure of CysA2 at 2.29 A shows two alpha/beta domains. The N domain is inactive, whereas the C domain is responsible for the catalytic activity. The conserved catalytic cysteine of the active site is Cys233 Mycobacterium tuberculosis
2.8.1.2 structure of CysA2 at 2.29 A shows two alpha/beta domains. The N domain is inactive, whereas the C domain is responsible for the catalytic activity. The conserved catalytic cysteine of the active site is Cys233 Mycobacterium tuberculosis

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.8.1.1 1.66
-
 cyanide pH 8.0, 37°C Mycobacterium tuberculosis
2.8.1.1 2.89
-
 thiosulfate pH 8.0, 37°C Mycobacterium tuberculosis
2.8.1.2 1.66
-
 cyanide pH 8.0, 37°C Mycobacterium tuberculosis
2.8.1.2 7.02
-
 3-Mercaptopyruvate pH 8.0, 37°C Mycobacterium tuberculosis

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.8.1.1 74000
-
 gel filtratioin Mycobacterium tuberculosis
2.8.1.2 74000
-
 gel filtratioin Mycobacterium tuberculosis

Organism

EC Number Organism UniProt Comment Textmining
2.8.1.1 Mycobacterium tuberculosis P9WHF9 cf. EC 2.8.1.2
-
2.8.1.1 Mycobacterium tuberculosis H37Rv P9WHF9 cf. EC 2.8.1.2
-
2.8.1.2 Mycobacterium tuberculosis P9WHF9 cf. EC 2.8.1.1
-
2.8.1.2 Mycobacterium tuberculosis H37Rv P9WHF9 cf. EC 2.8.1.1
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.8.1.1 3-mercaptopyruvate + cyanide
-
 Mycobacterium tuberculosis pyruvate + thiocyanate
-
 ?
2.8.1.1 3-mercaptopyruvate + cyanide
-
 Mycobacterium tuberculosis H37Rv pyruvate + thiocyanate
-
 ?
2.8.1.1 thiosulfate + cyanide
-
 Mycobacterium tuberculosis sulfite + thiocyanate
-
 ?
2.8.1.1 thiosulfate + cyanide
-
 Mycobacterium tuberculosis H37Rv sulfite + thiocyanate
-
 ?
2.8.1.2 3-mercaptopyruvate + cyanide
-
 Mycobacterium tuberculosis pyruvate + thiocyanate
-
 ?
2.8.1.2 3-mercaptopyruvate + cyanide
-
 Mycobacterium tuberculosis H37Rv pyruvate + thiocyanate
-
 ?
2.8.1.2 thiosulfate + cyanide
-
 Mycobacterium tuberculosis sulfite + thiocyanate
-
 ?
2.8.1.2 thiosulfate + cyanide
-
 Mycobacterium tuberculosis H37Rv sulfite + thiocyanate
-
 ?

Subunits

EC Number Subunits Comment Organism
2.8.1.1 dimer 2 * 31015, calculated from sequence Mycobacterium tuberculosis
2.8.1.2 dimer 2 * 31015, calculated from sequence Mycobacterium tuberculosis

Synonyms

EC Number Synonyms Comment Organism
2.8.1.1 CysA2
-
 Mycobacterium tuberculosis
2.8.1.1 Rv0815
-
 Mycobacterium tuberculosis
2.8.1.2 CysA2
-
 Mycobacterium tuberculosis
2.8.1.2 Rv0815
-
 Mycobacterium tuberculosis

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
2.8.1.1 58.3
-
 melting temperature Mycobacterium tuberculosis
2.8.1.2 58.3
-
 melting temperature Mycobacterium tuberculosis