Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Deobald, D.; Adrian, L.; Schöne, C.; Rother, M.; Layer, G.
    Identification of a unique Radical SAM methyltransferase required for the sp3-C-methylation of an arginine residue of methyl-coenzyme M reductase (2018), Sci. Rep., 8, 7404 .
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.8.4.1 genetic organization of the mcr operon, overview Methanosarcina acetivorans

Protein Variants

EC Number Protein Variants Comment Organism
2.8.4.1 additional information methylation mutant Mko4551 shows impaired growth under stress conditions Methanosarcina acetivorans

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.8.4.1 Ni2+ contained in the coenzyme F430 Methanosarcina acetivorans

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.1.1.379 2 S-adenosyl-L-methionine + a [methyl coenzyme-M reductase]-L-arginine285 + reduced acceptor Methanosarcina acetivorans
-
 S-adenosyl-L-homocysteine + L-methionine + 5'-deoxyadenosine + a [methyl coenzyme-M reductase]-(5S)-C-methyl-L-arginine285 + acceptor
-
 ?
2.1.1.379 2 S-adenosyl-L-methionine + a [methyl coenzyme-M reductase]-L-arginine285 + reduced acceptor Methanosarcina acetivorans DSM 2834
-
 S-adenosyl-L-homocysteine + L-methionine + 5'-deoxyadenosine + a [methyl coenzyme-M reductase]-(5S)-C-methyl-L-arginine285 + acceptor
-
 ?
2.8.4.1 methyl-CoM + CoB Methanosarcina acetivorans
-
 CoM-S-S-CoB + methane
-
 ?
2.8.4.1 methyl-CoM + CoB Methanosarcina acetivorans ATCC 35395
-
 CoM-S-S-CoB + methane
-
 ?
2.8.4.1 methyl-CoM + CoB Methanosarcina acetivorans DSM 2834
-
 CoM-S-S-CoB + methane
-
 ?
2.8.4.1 methyl-CoM + CoB Methanosarcina acetivorans JCM 12185
-
 CoM-S-S-CoB + methane
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.1.1.379 Methanosarcina acetivorans Q8THG6
-
-
2.1.1.379 Methanosarcina acetivorans DSM 2834 Q8THG6
-
-
2.8.4.1 Methanosarcina acetivorans Q8THH1 AND Q8THG7 AND Q8THH0 subunits A, B, and G; WWM1 and Mko4551 genotypes
-
2.8.4.1 Methanosarcina acetivorans ATCC 35395 Q8THH1 AND Q8THG7 AND Q8THH0 subunits A, B, and G; WWM1 and Mko4551 genotypes
-
2.8.4.1 Methanosarcina acetivorans DSM 2834 Q8THH1 AND Q8THG7 AND Q8THH0 subunits A, B, and G; WWM1 and Mko4551 genotypes
-
2.8.4.1 Methanosarcina acetivorans JCM 12185 Q8THH1 AND Q8THG7 AND Q8THH0 subunits A, B, and G; WWM1 and Mko4551 genotypes
-

Posttranslational Modification

EC Number Posttranslational Modification Comment Organism
2.8.4.1 alkylation enzyme MCR contains methylhistidine (His271) and methylcysteine (Cys472), as well as 5-C-(S)-methylarginine. A unique radical SAM methyltransferase is required for the sp3-C-methylation of the arginine residue of methyl-coenzyme M reductase, the a 5-C-(S)-methylarginine is located close to the active site of the enzyme. Deletion of the methyltransferase gene ma4551 in Methanosarcina acetivorans strain WWM1 leads to the production of an active MCR lacking the C-5-methylation of the respective arginine residue. The methylated arginine is important for MCR stability under stress conditions Identification of potential radical SAM methyltransferase genes in the genomes of methanogens, overview Methanosarcina acetivorans
2.8.4.1 additional information four amino acid modifications are reported to be present in McrA, namely methylhistidine (His271), methylcysteine (Cys472), thioglycine (Gly465) and didehydroaspartate (Asp470). Mass spectrometric analysis Methanosarcina acetivorans
2.8.4.1 side-chain modification the enzyme contains thioglycine (Gly465) and didehydroaspartate (Asp470) Methanosarcina acetivorans

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.1.1.379 2 S-adenosyl-L-methionine + a [methyl coenzyme-M reductase]-L-arginine285 + reduced acceptor
-
 Methanosarcina acetivorans S-adenosyl-L-homocysteine + L-methionine + 5'-deoxyadenosine + a [methyl coenzyme-M reductase]-(5S)-C-methyl-L-arginine285 + acceptor
-
 ?
2.1.1.379 2 S-adenosyl-L-methionine + a [methyl coenzyme-M reductase]-L-arginine285 + reduced acceptor
-
 Methanosarcina acetivorans DSM 2834 S-adenosyl-L-homocysteine + L-methionine + 5'-deoxyadenosine + a [methyl coenzyme-M reductase]-(5S)-C-methyl-L-arginine285 + acceptor
-
 ?
2.8.4.1 methyl-CoM + CoB
-
 Methanosarcina acetivorans CoM-S-S-CoB + methane
-
 ?
2.8.4.1 methyl-CoM + CoB
-
 Methanosarcina acetivorans ATCC 35395 CoM-S-S-CoB + methane
-
 ?
2.8.4.1 methyl-CoM + CoB
-
 Methanosarcina acetivorans DSM 2834 CoM-S-S-CoB + methane
-
 ?
2.8.4.1 methyl-CoM + CoB
-
 Methanosarcina acetivorans JCM 12185 CoM-S-S-CoB + methane
-
 ?

Synonyms

EC Number Synonyms Comment Organism
2.1.1.379 MA4551
-
 Methanosarcina acetivorans
2.1.1.379 methanogenesis marker 10
-
 Methanosarcina acetivorans
2.8.4.1 MCR
-
 Methanosarcina acetivorans
2.8.4.1 mcrA
-
 Methanosarcina acetivorans
2.8.4.1 mcrA subunit A Methanosarcina acetivorans
2.8.4.1 mcrB
-
 Methanosarcina acetivorans
2.8.4.1 mcrB subunit B Methanosarcina acetivorans
2.8.4.1 mcrG
-
 Methanosarcina acetivorans
2.8.4.1 mcrG subunit G Methanosarcina acetivorans
2.8.4.1 methyl-coenzyme M reductase
-
 Methanosarcina acetivorans

Cofactor

EC Number Cofactor Comment Organism Structure
2.8.4.1 F-430 coenzyme F430 Methanosarcina acetivorans

General Information

EC Number General Information Comment Organism
2.1.1.379 malfunction enzyme knockout strains show impaired growth under stress conditions such as the presence of 0.2 mM hydrogen peroxide or elevated temperature (42°C) Methanosarcina acetivorans
2.8.4.1 malfunction methylation mutant Mko4551 shows impaired growth under stress conditions Methanosarcina acetivorans
2.8.4.1 metabolism the last and methane-releasing step of methanogenesis is catalysed by the enzyme methyl-coenzyme M reductase (MCR) Methanosarcina acetivorans