EC Number | Activating Compound | Comment | Organism | Structure |
---|---|---|---|---|
1.1.1.41 | ADP | - |
Homo sapiens | |
1.1.1.41 | citrate | - |
Homo sapiens |
EC Number | Cloned (Comment) | Organism |
---|---|---|
1.1.1.41 | expressed in Escherichia coli BL21(DE3) Codon-Plus cells | Homo sapiens |
EC Number | Crystallization (Comment) | Organism |
---|---|---|
1.1.1.41 | alphagamma heterodimer bound with an Mg2+ and an NADH at the active site and an NADH at the allosteric site, hanging drop vapor diffusion method, using 0.1 M HEPES (pH 7.5), 50 mM MgCl2, and 30% (w/v) PEGMME 550 | Homo sapiens |
EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
1.1.1.41 | NADH | NADH at the active site occupies the binding site for NAD+ and prevents the binding of the cofactor. NADH at the allosteric site occupies the binding sites for ADP and citrate and blocks the binding of the activators. NADH inhibits the activities of the alphagamma, alphabeta and alpha2betagamma enzyme forms | Homo sapiens |
EC Number | Metals/Ions | Comment | Organism | Structure |
---|---|---|---|---|
1.1.1.41 | Mn2+ | 2 mM used in assay conditions | Homo sapiens |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.1.1.41 | isocitrate + NAD+ | Homo sapiens | - |
2-oxoglutarate + CO2 + NADH + H+ | - |
? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
1.1.1.41 | Homo sapiens | P50213 and O43837 and P51553 | subunits alpha, beta and gamma | - |
EC Number | Purification (Comment) | Organism |
---|---|---|
1.1.1.41 | Ni-NTA column chromatography and Superdex 200 gel filtration | Homo sapiens |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.1.1.41 | isocitrate + NAD+ | - |
Homo sapiens | 2-oxoglutarate + CO2 + NADH + H+ | - |
? |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
1.1.1.41 | heterotetramer | - |
Homo sapiens |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
1.1.1.41 | NAD-dependent isocitrate dehydrogenase | - |
Homo sapiens |
1.1.1.41 | NAD-IDH | - |
Homo sapiens |
EC Number | Cofactor | Comment | Organism | Structure |
---|---|---|---|---|
1.1.1.41 | NAD+ | - |
Homo sapiens |
EC Number | IC50 Value | IC50 Value Maximum | Comment | Organism | Inhibitor | Structure |
---|---|---|---|---|---|---|
1.1.1.41 | 0.0785 | - |
alpha2betagamma heterotetramer, at pH 7.4 and 25°C | Homo sapiens | NADH | |
1.1.1.41 | 0.0827 | - |
alphagamma heterodimer, at pH 7.4 and 25°C | Homo sapiens | NADH | |
1.1.1.41 | 0.0949 | - |
alphabeta heterodimer, at pH 7.4 and 25°C | Homo sapiens | NADH |