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Literature summary extracted from

  • Liu, Y.; Hu, L.; Ma, T.; Yang, J.; Ding, J.
    Insights into the inhibitory mechanisms of NADH on the alphagamma heterodimer of human NAD-dependent isocitrate dehydrogenase (2018), Sci. Rep., 8, 3146 .
    View publication on PubMedView publication on EuropePMC

Activating Compound

EC Number Activating Compound Comment Organism Structure
1.1.1.41 ADP
-
Homo sapiens
1.1.1.41 citrate
-
Homo sapiens

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.1.1.41 expressed in Escherichia coli BL21(DE3) Codon-Plus cells Homo sapiens

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
1.1.1.41 alphagamma heterodimer bound with an Mg2+ and an NADH at the active site and an NADH at the allosteric site, hanging drop vapor diffusion method, using 0.1 M HEPES (pH 7.5), 50 mM MgCl2, and 30% (w/v) PEGMME 550 Homo sapiens

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.1.1.41 NADH NADH at the active site occupies the binding site for NAD+ and prevents the binding of the cofactor. NADH at the allosteric site occupies the binding sites for ADP and citrate and blocks the binding of the activators. NADH inhibits the activities of the alphagamma, alphabeta and alpha2betagamma enzyme forms Homo sapiens

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.1.1.41 Mn2+ 2 mM used in assay conditions Homo sapiens

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.1.1.41 isocitrate + NAD+ Homo sapiens
-
2-oxoglutarate + CO2 + NADH + H+
-
?

Organism

EC Number Organism UniProt Comment Textmining
1.1.1.41 Homo sapiens P50213 and O43837 and P51553 subunits alpha, beta and gamma
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.1.1.41 Ni-NTA column chromatography and Superdex 200 gel filtration Homo sapiens

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.1.41 isocitrate + NAD+
-
Homo sapiens 2-oxoglutarate + CO2 + NADH + H+
-
?

Subunits

EC Number Subunits Comment Organism
1.1.1.41 heterotetramer
-
Homo sapiens

Synonyms

EC Number Synonyms Comment Organism
1.1.1.41 NAD-dependent isocitrate dehydrogenase
-
Homo sapiens
1.1.1.41 NAD-IDH
-
Homo sapiens

Cofactor

EC Number Cofactor Comment Organism Structure
1.1.1.41 NAD+
-
Homo sapiens

IC50 Value

EC Number IC50 Value IC50 Value Maximum Comment Organism Inhibitor Structure
1.1.1.41 0.0785
-
alpha2betagamma heterotetramer, at pH 7.4 and 25°C Homo sapiens NADH
1.1.1.41 0.0827
-
alphagamma heterodimer, at pH 7.4 and 25°C Homo sapiens NADH
1.1.1.41 0.0949
-
alphabeta heterodimer, at pH 7.4 and 25°C Homo sapiens NADH