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Literature summary extracted from

  • Sun, P.; Bai, T.; Ma, T.; Ding, J.
    Molecular mechanism of the dual regulatory roles of ATP on the alphagamma heterodimer of human NAD-dependent isocitrate dehydrogenase (2020), Sci. Rep., 10, 6225 .
    View publication on PubMedView publication on EuropePMC

Activating Compound

EC Number Activating Compound Comment Organism Structure
1.1.1.41 ATP the enzyme can be activated by low concentrations of ATP. At high concentrations ATP is competitive with NAD+ for binding to the catalytic site Homo sapiens
1.1.1.41 citrate
-
 Homo sapiens

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.1.1.41 expressed in Escherichia coli BL21(DE3) CodonPlus cells Homo sapiens

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
1.1.1.41 alphagamma heterodimer with the active site bound with either Mg2+ alone or Mg2+ and ATP, and the allosteric site bound with Mg2+, citrate and ATP, hanging drop vapor diffusion method, using 0.2 M sodium citrate, pH 8.0, and 20% (w/v) PEG3350 or 0.2 M sodium citrate, pH 5.5, and 24% (v/v) PEG400 Homo sapiens

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.1.1.41 NADH
-
 Homo sapiens

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
1.1.1.41 mitochondrion
-
 Homo sapiens 5739
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.1.1.41 Mg2+ activates Homo sapiens
1.1.1.41 Mn2+ 2 mM used in assay conditions Homo sapiens

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.1.1.41 isocitrate + NAD+ Homo sapiens
-
 2-oxoglutarate + CO2 + NADH + H+
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.1.1.41 Homo sapiens P50213 and O43837 and P51553 subunits alpha, beta and gamma
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.1.1.41 Ni-NTA column chromatography and Superdex 200 gel filtration Homo sapiens

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.1.41 isocitrate + NAD+
-
 Homo sapiens 2-oxoglutarate + CO2 + NADH + H+
-
 ?

Subunits

EC Number Subunits Comment Organism
1.1.1.41 heterotetramer 1 * 39000 + 1 * 39000 + 2 * 37000, SDS-PAGE Homo sapiens

Synonyms

EC Number Synonyms Comment Organism
1.1.1.41 IDH3
-
 Homo sapiens
1.1.1.41 NAD-dependent isocitrate dehydrogenase
-
 Homo sapiens
1.1.1.41 NAD-IDH
-
 Homo sapiens

Cofactor

EC Number Cofactor Comment Organism Structure
1.1.1.41 NAD+
-
 Homo sapiens