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Literature summary extracted from

  • Kuritani, Y.; Sato, K.; Dohra, H.; Umemura, S.; Kitaoka, M.; Fushinobu, S.; Yoshida, N.
    Conversion of levoglucosan into glucose by the coordination of four enzymes through oxidation, elimination, hydration, and reduction (2020), Sci. Rep., 10, 20066 .
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.1.1.425 expression in Escherichia coli Bacillus smithii

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.1.1.425 3-dehydrolevoglucosan + NADH + H+ Bacillus smithii
-
 levoglucosan + NAD+
-
 r
1.1.1.425 3-dehydrolevoglucosan + NADH + H+ Bacillus smithii S-2701M
-
 levoglucosan + NAD+
-
 r
1.1.1.425 levoglucosan + NAD+ Bacillus smithii
-
 3-dehydrolevoglucosan + NADH + H+
-
 r
1.1.1.425 levoglucosan + NAD+ Bacillus smithii S-2701M
-
 3-dehydrolevoglucosan + NADH + H+
-
 r

Organism

EC Number Organism UniProt Comment Textmining
1.1.1.425 Bacillus smithii
-
-
-
1.1.1.425 Bacillus smithii S-2701M
-
-
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.1.1.425 1.32
-
 substrate levoglucosan, pH 7.5, 30°C Bacillus smithii
1.1.1.425 6
-
 substrate 3-dehydrolevoglucosan, pH 7.5, 30°C Bacillus smithii

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.1.425 3-dehydrolevoglucosan + NADH + H+
-
 Bacillus smithii levoglucosan + NAD+
-
 r
1.1.1.425 3-dehydrolevoglucosan + NADH + H+
-
 Bacillus smithii S-2701M levoglucosan + NAD+
-
 r
1.1.1.425 levoglucosan + NAD+
-
 Bacillus smithii 3-dehydrolevoglucosan + NADH + H+
-
 r
1.1.1.425 levoglucosan + NAD+
-
 Bacillus smithii S-2701M 3-dehydrolevoglucosan + NADH + H+
-
 r

Subunits

EC Number Subunits Comment Organism
1.1.1.425 ? x * 44000, SDS-PAGE Bacillus smithii

Synonyms

EC Number Synonyms Comment Organism
1.1.1.425 LgdA
-
 Bacillus smithii
1.1.1.425 LgdH
-
 Bacillus smithii

General Information

EC Number General Information Comment Organism
1.1.1.425 metabolism in the metabolic pathway from levoglucosan to glucose, LgdA (LGDH) catalyzes 3-dehydrogenation of levoglucosan to produce 3-keto-levoglucosan, which undergoes beta-elimination of 3-keto-levoglucosan by LgdB1, followed by hydration to produce 3-keto-D-glucose by LgdB2. Next, LgdC reduces 3-keto-D-glucose to glucose Bacillus smithii