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Literature summary extracted from

  • Lee, Y.; Choi, O.; Kang, B.; Bae, J.; Kim, S.; Kim, J.
    Grey mould control by oxalate degradation using non-antifungal Pseudomonas abietaniphila strain ODB36 (2020), Sci. Rep., 10, 1605 .
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.8.3.16 gene podA, DNA and amino acid sequence determination and analysis, sequence comparisons and phylogenetic analysis, recombinant expression of His-tagged enzyme in Escherichia coli strain BL21(DE3) Pseudomonas abietaniphila

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.8.3.16 formyl-CoA + oxalate Pseudomonas abietaniphila
-
 formate + oxalyl-CoA
-
 ?
2.8.3.16 formyl-CoA + oxalate Pseudomonas abietaniphila ODB36
-
 formate + oxalyl-CoA
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.8.3.16 Pseudomonas abietaniphila A0A1G7S280 isolated from surfaces of strawberries and spinach
-
2.8.3.16 Pseudomonas abietaniphila ODB36 A0A1G7S280 isolated from surfaces of strawberries and spinach
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.8.3.16 recombinant His-tagged enzyme from Escherichia coli strain BL21(DE3) by nickel affinity chromatography and dialysis, to near homogeneity Pseudomonas abietaniphila

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.8.3.16 formyl-CoA + oxalate
-
 Pseudomonas abietaniphila formate + oxalyl-CoA
-
 ?
2.8.3.16 formyl-CoA + oxalate
-
 Pseudomonas abietaniphila ODB36 formate + oxalyl-CoA
-
 ?

Subunits

EC Number Subunits Comment Organism
2.8.3.16 ? x * 42000, recombinant His-tagged enzyme, SDS-PAGE Pseudomonas abietaniphila

Synonyms

EC Number Synonyms Comment Organism
2.8.3.16 podA
-
 Pseudomonas abietaniphila

General Information

EC Number General Information Comment Organism
2.8.3.16 malfunction the podA- mutant does not inhibit Botrytis infection and oxalate toxicity, the defects are recovered by podA complementation Pseudomonas abietaniphila
2.8.3.16 physiological function PodA converts oxalate to formate and eliminates oxalate toxicity. Oxalate is a key component of virulent necrotic fungal pathogens. Pseudomonas abietaniphila strain ODB36 exhibits significant plant protective ability, e.g. against Bortrytis cinerea, which causes grey mould disease, on Arabidopsis thaliana (Col-0) leaves. PodA is required for the inhibition of Botrytis infection. Degrading oxalate at the onset of host pathogen interaction blocks the infection process Pseudomonas abietaniphila