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Literature summary extracted from

  • Pei, X.; Wang, J.; Zheng, H.; Cheng, P.; Wu, Y.; Wang, A.; Su, W.
    Highly efficient asymmetric reduction of ketopantolactone to D-(-)-pantolactone by Escherichia coli cells expressing recombinant conjugated polyketone reductase and glucose dehydrogenase in a fed-batch biphasic reaction system (2020), React. Chem. Eng., 5, 531-538 .
No PubMed abstract available

Application

EC Number Application Comment Organism
1.1.1.214 synthesis whole-cell biotransformation process to produce D-pantolactone in a biphasic reaction system. Recombinant CPR and glucose dehydrogenase are co-expressed in Escherichia coli to simultaneously achieve the synthesis of D-PL and the regeneration of NADPH. Presence of 15% dichloromethane significantly inhibits the hydrolysis of ketopantolactone. In a fed-batch system, the D-pantolactone concentration reaches 0.77 mol per l in the reaction mixture at 7 h, and its enantiomeric excess is 99% Candida dubliniensis

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.1.1.169 co-expressed with glucose dehydrogenase from Bacillus subtilis in Escherichia coli BL21(DE3) cells Candida dubliniensis

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.1.1.169 ketopantolactone + NADPH + H+ Candida dubliniensis
-
 D-(-)-pantolactone + NADP+
-
 ?
1.1.1.169 ketopantolactone + NADPH + H+ Candida dubliniensis CD36
-
 D-(-)-pantolactone + NADP+
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.1.1.169 Candida dubliniensis
-
-
-
1.1.1.169 Candida dubliniensis CD36
-
-
-
1.1.1.214 Candida dubliniensis B9WJQ5
-
-
1.1.1.214 Candida dubliniensis CD36 B9WJQ5
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.1.169 ketopantolactone + NADPH + H+
-
 Candida dubliniensis D-(-)-pantolactone + NADP+
-
 ?
1.1.1.169 ketopantolactone + NADPH + H+
-
 Candida dubliniensis CD36 D-(-)-pantolactone + NADP+
-
 ?
1.1.1.214 (R)-pantolactone + NADP+
-
 Candida dubliniensis 2-dehydropantolactone + NADPH + H+
-
 ?
1.1.1.214 (R)-pantolactone + NADP+
-
 Candida dubliniensis CD36 2-dehydropantolactone + NADPH + H+
-
 ?

Synonyms

EC Number Synonyms Comment Organism
1.1.1.169 conjugated polyketone reductase
-
 Candida dubliniensis
1.1.1.169 CPR
-
 Candida dubliniensis
1.1.1.214 CPR-C2
-
 Candida dubliniensis

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.1.1.169 6.5
-
-
 Candida dubliniensis

Cofactor

EC Number Cofactor Comment Organism Structure
1.1.1.169 NADPH
-
 Candida dubliniensis