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Literature summary extracted from

  • Vanasi, B.; Eppakayala, L.; Malothu, R.
    L-lysine production by chemical mutagenesis of homoserine dehydrogenase of ddh gene in Corynebacterium glutamicum ATCC13032 (2020), Rasayan J. Chem., 13, 1245-1261 .
No PubMed abstract available

Protein Variants

EC Number Protein Variants Comment Organism
1.1.1.3 additional information generation of a knockout homoserine dehydrogenase (HSD) mutant by chemical mutagenesis. Auxotrophic mutant formed from ddh gene, encoding diaminopimelate dehydrogenase, recombinantly expressed in Corynebacterium glutamicum strain ATCC 13032 with blocked HSD shows increased yield of L-lysine of 24.89 g/l compared to ddh gene expressed in wild-type strain ATCC 13032 (20.66 g/l of L-lysine). The maximum yield of L-lysine for the auxotrophic mutant is attained at pH 7.5 and 30°C after 96 h incubation time. Method optimization, overview Corynebacterium glutamicum

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.1.1.3 L-homoserine + NAD+ Corynebacterium glutamicum
-
 L-aspartate 4-semialdehyde + NADH + H+
-
 ?
1.1.1.3 L-homoserine + NAD+ Corynebacterium glutamicum LMG 3730
-
 L-aspartate 4-semialdehyde + NADH + H+
-
 ?
1.1.1.3 L-homoserine + NAD+ Corynebacterium glutamicum BCRC 11384
-
 L-aspartate 4-semialdehyde + NADH + H+
-
 ?
1.1.1.3 L-homoserine + NAD+ Corynebacterium glutamicum ATCC 13032
-
 L-aspartate 4-semialdehyde + NADH + H+
-
 ?
1.1.1.3 L-homoserine + NAD+ Corynebacterium glutamicum JCM 1318
-
 L-aspartate 4-semialdehyde + NADH + H+
-
 ?
1.1.1.3 L-homoserine + NAD+ Corynebacterium glutamicum NCIMB 10025
-
 L-aspartate 4-semialdehyde + NADH + H+
-
 ?
1.1.1.3 L-homoserine + NAD+ Corynebacterium glutamicum DSM 20300
-
 L-aspartate 4-semialdehyde + NADH + H+
-
 ?
1.1.1.3 L-homoserine + NADP+ Corynebacterium glutamicum
-
 L-aspartate 4-semialdehyde + NADPH + H+
-
 ?
1.1.1.3 L-homoserine + NADP+ Corynebacterium glutamicum LMG 3730
-
 L-aspartate 4-semialdehyde + NADPH + H+
-
 ?
1.1.1.3 L-homoserine + NADP+ Corynebacterium glutamicum BCRC 11384
-
 L-aspartate 4-semialdehyde + NADPH + H+
-
 ?
1.1.1.3 L-homoserine + NADP+ Corynebacterium glutamicum ATCC 13032
-
 L-aspartate 4-semialdehyde + NADPH + H+
-
 ?
1.1.1.3 L-homoserine + NADP+ Corynebacterium glutamicum JCM 1318
-
 L-aspartate 4-semialdehyde + NADPH + H+
-
 ?
1.1.1.3 L-homoserine + NADP+ Corynebacterium glutamicum NCIMB 10025
-
 L-aspartate 4-semialdehyde + NADPH + H+
-
 ?
1.1.1.3 L-homoserine + NADP+ Corynebacterium glutamicum DSM 20300
-
 L-aspartate 4-semialdehyde + NADPH + H+
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.1.1.3 Corynebacterium glutamicum P08499
-
-
1.1.1.3 Corynebacterium glutamicum ATCC 13032 P08499
-
-
1.1.1.3 Corynebacterium glutamicum BCRC 11384 P08499
-
-
1.1.1.3 Corynebacterium glutamicum DSM 20300 P08499
-
-
1.1.1.3 Corynebacterium glutamicum JCM 1318 P08499
-
-
1.1.1.3 Corynebacterium glutamicum LMG 3730 P08499
-
-
1.1.1.3 Corynebacterium glutamicum NCIMB 10025 P08499
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.1.3 L-homoserine + NAD+
-
 Corynebacterium glutamicum L-aspartate 4-semialdehyde + NADH + H+
-
 ?
1.1.1.3 L-homoserine + NAD+
-
 Corynebacterium glutamicum LMG 3730 L-aspartate 4-semialdehyde + NADH + H+
-
 ?
1.1.1.3 L-homoserine + NAD+
-
 Corynebacterium glutamicum BCRC 11384 L-aspartate 4-semialdehyde + NADH + H+
-
 ?
1.1.1.3 L-homoserine + NAD+
-
 Corynebacterium glutamicum ATCC 13032 L-aspartate 4-semialdehyde + NADH + H+
-
 ?
1.1.1.3 L-homoserine + NAD+
-
 Corynebacterium glutamicum JCM 1318 L-aspartate 4-semialdehyde + NADH + H+
-
 ?
1.1.1.3 L-homoserine + NAD+
-
 Corynebacterium glutamicum NCIMB 10025 L-aspartate 4-semialdehyde + NADH + H+
-
 ?
1.1.1.3 L-homoserine + NAD+
-
 Corynebacterium glutamicum DSM 20300 L-aspartate 4-semialdehyde + NADH + H+
-
 ?
1.1.1.3 L-homoserine + NADP+
-
 Corynebacterium glutamicum L-aspartate 4-semialdehyde + NADPH + H+
-
 ?
1.1.1.3 L-homoserine + NADP+
-
 Corynebacterium glutamicum LMG 3730 L-aspartate 4-semialdehyde + NADPH + H+
-
 ?
1.1.1.3 L-homoserine + NADP+
-
 Corynebacterium glutamicum BCRC 11384 L-aspartate 4-semialdehyde + NADPH + H+
-
 ?
1.1.1.3 L-homoserine + NADP+
-
 Corynebacterium glutamicum ATCC 13032 L-aspartate 4-semialdehyde + NADPH + H+
-
 ?
1.1.1.3 L-homoserine + NADP+
-
 Corynebacterium glutamicum JCM 1318 L-aspartate 4-semialdehyde + NADPH + H+
-
 ?
1.1.1.3 L-homoserine + NADP+
-
 Corynebacterium glutamicum NCIMB 10025 L-aspartate 4-semialdehyde + NADPH + H+
-
 ?
1.1.1.3 L-homoserine + NADP+
-
 Corynebacterium glutamicum DSM 20300 L-aspartate 4-semialdehyde + NADPH + H+
-
 ?

Synonyms

EC Number Synonyms Comment Organism
1.1.1.3 hom
-
 Corynebacterium glutamicum

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.1.1.3 30
-
 assay at Corynebacterium glutamicum

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.1.1.3 7.5
-
 assay at Corynebacterium glutamicum

Cofactor

EC Number Cofactor Comment Organism Structure
1.1.1.3 NAD+
-
 Corynebacterium glutamicum
1.1.1.3 NADH
-
 Corynebacterium glutamicum
1.1.1.3 NADP+
-
 Corynebacterium glutamicum
1.1.1.3 NADPH
-
 Corynebacterium glutamicum

General Information

EC Number General Information Comment Organism
1.1.1.3 malfunction the chemical mutagen ENU causes a mutation in the homoserine serine dehydrogenase enzyme which diverted the aspartyl-beta-semialdehyde to bind with 2,3-dihydrodipicolinate synthase to participate in the L-lysine synthesis through 2,3 meso-diaminopimelate (Meso-Dap). Being a recombinant for diaminopimelate dehydrogenase (ddh), the auxotrophic mutant for the homoserine dehydrogenase follows the ddh pathway by overexpression of ddh by deviating the acetyltransferase and succinyl transferase is the reason for the high yield of L-lysine production Corynebacterium glutamicum
1.1.1.3 additional information molecular docking analysis of aspartyl-beta-semi aldehyde with homoserine dehydrogenase, modeling Corynebacterium glutamicum