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Literature summary extracted from

  • Ohshida, T.; Hayashi, J.; Yoneda, K.; Ohshima, T.; Sakuraba, H.
    Unique active site formation in a novel galactose 1-phosphate uridylyltransferase from the hyperthermophilic archaeon Pyrobaculum aerophilum (2020), Proteins, 88, 669-678 .
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.7.7.12 overexpressed in Escherichia coli Pyrobaculum aerophilum

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
2.7.7.12 sitting-drop vapor diffusion method, two different crystal structures of Pyrobaculum aerophilum galactose 1-phosphate uridylyltransferase (GalT) are determined: the substrate-free enzyme at 2.33 A and the UDP-bound H140F mutant enzyme at 1.78 A Pyrobaculum aerophilum

Protein Variants

EC Number Protein Variants Comment Organism
2.7.7.12 H140F inactive mutant enzyme Pyrobaculum aerophilum
2.7.7.12 H140G inactive mutant enzyme Pyrobaculum aerophilum
2.7.7.12 H140N inactive mutant enzyme Pyrobaculum aerophilum

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.7.7.12 0.084
-
 alpha-D-galactose 1-phosphate pH 7.5, 50°C Pyrobaculum aerophilum
2.7.7.12 0.73
-
 UDP-alpha-D-glucose pH 7.5, 50°C Pyrobaculum aerophilum

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.7.7.12 UDP-alpha-D-glucose + alpha-D-galactose 1-phosphate Pyrobaculum aerophilum the enzyme is involved in galactose metabolism via the Leloir pathway alpha-D-glucose 1-phosphate + UDP-alpha-D-galactose
-
 ?
2.7.7.12 UDP-alpha-D-glucose + alpha-D-galactose 1-phosphate Pyrobaculum aerophilum ATCC 51768 the enzyme is involved in galactose metabolism via the Leloir pathway alpha-D-glucose 1-phosphate + UDP-alpha-D-galactose
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.7.7.12 Pyrobaculum aerophilum Q8ZXN7
-
-
2.7.7.12 Pyrobaculum aerophilum ATCC 51768 Q8ZXN7
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.7.7.12
-
 Pyrobaculum aerophilum

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.7.12 UDP-alpha-D-glucose + alpha-D-galactose 1-phosphate the enzyme is involved in galactose metabolism via the Leloir pathway Pyrobaculum aerophilum alpha-D-glucose 1-phosphate + UDP-alpha-D-galactose
-
 ?
2.7.7.12 UDP-alpha-D-glucose + alpha-D-galactose 1-phosphate binding of the glucose moiety of the substrate, but not the UDP moiety, gives rise to a large structural change around the active site Pyrobaculum aerophilum alpha-D-glucose 1-phosphate + UDP-alpha-D-galactose
-
 ?
2.7.7.12 UDP-alpha-D-glucose + alpha-D-galactose 1-phosphate the enzyme is involved in galactose metabolism via the Leloir pathway Pyrobaculum aerophilum ATCC 51768 alpha-D-glucose 1-phosphate + UDP-alpha-D-galactose
-
 ?
2.7.7.12 UDP-alpha-D-glucose + alpha-D-galactose 1-phosphate binding of the glucose moiety of the substrate, but not the UDP moiety, gives rise to a large structural change around the active site Pyrobaculum aerophilum ATCC 51768 alpha-D-glucose 1-phosphate + UDP-alpha-D-galactose
-
 ?

Synonyms

EC Number Synonyms Comment Organism
2.7.7.12 galactose 1-phosphate uridylyltransferase
-
 Pyrobaculum aerophilum
2.7.7.12 GALT
-
 Pyrobaculum aerophilum

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
2.7.7.12 90
-
 10 min, the enzyme retains about 90% of its activity Pyrobaculum aerophilum

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.7.7.12 7.5
-
-
 Pyrobaculum aerophilum

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
2.7.7.12 5 10 no loss of the activity when incubated between pH 5.0 and pH 10.0 for 10 minutes at 50°C Pyrobaculum aerophilum

General Information

EC Number General Information Comment Organism
2.7.7.12 metabolism the enzyme is involved in galactose metabolism via the Leloir pathway Pyrobaculum aerophilum