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Literature summary extracted from

  • Takahashi, D.; Suzuki, K.; Sakamoto, T.; Iwamoto, T.; Murata, T.; Sakane, F.
    Crystal structure and calcium-induced conformational changes of diacylglycerol kinase alpha EF-hand domains (2019), Protein Sci., 28, 694-706 .
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.7.1.107 expressed in Escherichia coli Rosetta2 (DE3) cells Homo sapiens

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
2.7.1.107 Ca2+-bound EF-hand domain of isoform DGKalpha, sitting drop vapor diffusion method, using 0.1 M HEPES, pH 7.0, 1.0 M succinic acid, and 1% (w/v) PEG monomethyl ether 2000 Homo sapiens

Protein Variants

EC Number Protein Variants Comment Organism
2.7.1.107 E134Q the mutant shows impaired Ca2+ binding Homo sapiens
2.7.1.107 E179Q the mutant is not able to bind Ca2+ Homo sapiens

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.7.1.107 Ca2+ the enzyme is positively regulated by Ca2+ binding to its N-terminal EF-hand domains. Cooperative binding of two Ca2+ ions dissociates the dimer into a well-folded monomer, which resists to proteolysis Homo sapiens

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.7.1.107 ATP + 1,2-diacyl-sn-glycerol Homo sapiens
-
ADP + 1,2-diacyl-sn-glycerol 3-phosphate
-
?

Organism

EC Number Organism UniProt Comment Textmining
2.7.1.107 Homo sapiens P23743
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.7.1.107 Ni-NTA agarose column chromatography, Superdex 75 gel filtration and Superdex 200 gel filtration Homo sapiens

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.1.107 ATP + 1,2-diacyl-sn-glycerol
-
Homo sapiens ADP + 1,2-diacyl-sn-glycerol 3-phosphate
-
?

Subunits

EC Number Subunits Comment Organism
2.7.1.107 monomer 1 * 10000, isoform DGKalpha-EF domain, SDS-PAGE Homo sapiens
2.7.1.107 monomer 1 * 10600, isoform DGKalpha-EF domain, calculated from amino acid sequence Homo sapiens

Synonyms

EC Number Synonyms Comment Organism
2.7.1.107 DGKalpha isoform Homo sapiens
2.7.1.107 diacylglycerol kinase alpha isoform Homo sapiens