Literature summary extracted from
Takahashi, D.; Suzuki, K.; Sakamoto, T.; Iwamoto, T.; Murata, T.; Sakane, F.
Crystal structure and calcium-induced conformational changes of diacylglycerol kinase alpha EF-hand domains (2019), Protein Sci., 28, 694-706 .
Cloned(Commentary)
EC Number |
Cloned (Comment) |
Organism |
---|
2.7.1.107 |
expressed in Escherichia coli Rosetta2 (DE3) cells |
Homo sapiens |
Crystallization (Commentary)
EC Number |
Crystallization (Comment) |
Organism |
---|
2.7.1.107 |
Ca2+-bound EF-hand domain of isoform DGKalpha, sitting drop vapor diffusion method, using 0.1 M HEPES, pH 7.0, 1.0 M succinic acid, and 1% (w/v) PEG monomethyl ether 2000 |
Homo sapiens |
Protein Variants
EC Number |
Protein Variants |
Comment |
Organism |
---|
2.7.1.107 |
E134Q |
the mutant shows impaired Ca2+ binding |
Homo sapiens |
2.7.1.107 |
E179Q |
the mutant is not able to bind Ca2+ |
Homo sapiens |
Metals/Ions
EC Number |
Metals/Ions |
Comment |
Organism |
Structure |
---|
2.7.1.107 |
Ca2+ |
the enzyme is positively regulated by Ca2+ binding to its N-terminal EF-hand domains. Cooperative binding of two Ca2+ ions dissociates the dimer into a well-folded monomer, which resists to proteolysis |
Homo sapiens |
|
Natural Substrates/ Products (Substrates)
EC Number |
Natural Substrates |
Organism |
Comment (Nat. Sub.) |
Natural Products |
Comment (Nat. Pro.) |
Rev. |
Reac. |
---|
2.7.1.107 |
ATP + 1,2-diacyl-sn-glycerol |
Homo sapiens |
- |
ADP + 1,2-diacyl-sn-glycerol 3-phosphate |
- |
? |
|
Organism
EC Number |
Organism |
UniProt |
Comment |
Textmining |
---|
2.7.1.107 |
Homo sapiens |
P23743 |
- |
- |
Purification (Commentary)
EC Number |
Purification (Comment) |
Organism |
---|
2.7.1.107 |
Ni-NTA agarose column chromatography, Superdex 75 gel filtration and Superdex 200 gel filtration |
Homo sapiens |
Substrates and Products (Substrate)
EC Number |
Substrates |
Comment Substrates |
Organism |
Products |
Comment (Products) |
Rev. |
Reac. |
---|
2.7.1.107 |
ATP + 1,2-diacyl-sn-glycerol |
- |
Homo sapiens |
ADP + 1,2-diacyl-sn-glycerol 3-phosphate |
- |
? |
|
Subunits
EC Number |
Subunits |
Comment |
Organism |
---|
2.7.1.107 |
monomer |
1 * 10000, isoform DGKalpha-EF domain, SDS-PAGE |
Homo sapiens |
2.7.1.107 |
monomer |
1 * 10600, isoform DGKalpha-EF domain, calculated from amino acid sequence |
Homo sapiens |
Synonyms
EC Number |
Synonyms |
Comment |
Organism |
---|
2.7.1.107 |
DGKalpha |
isoform |
Homo sapiens |
2.7.1.107 |
diacylglycerol kinase alpha |
isoform |
Homo sapiens |