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Literature summary extracted from

  • Bacot-Davis, V.R.; Bassenden, A.V.; Sprules, T.; Berghuis, A.M.
    Effect of solvent and protein dynamics in ligand recognition and inhibition of aminoglycoside adenyltransferase 2''-Ia (2017), Protein Sci., 26, 1852-1863 .
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.7.7.46 expressed in Escherichia coli BL21(DE3) cells Klebsiella pneumoniae

Protein Variants

EC Number Protein Variants Comment Organism
2.7.7.46 D44A the mutation causes a 4fold decrease in aminoglycoside adenylation compared to the wild type enzyme Klebsiella pneumoniae
2.7.7.46 I129A the mutation causes a 3fold decrease in aminoglycoside adenylation compared to the wild type enzyme Klebsiella pneumoniae
2.7.7.46 Y135A the mutation causes a 1.6fold decrease in aminoglycoside adenylation compared to the wild type enzyme Klebsiella pneumoniae

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.7.7.46 Mg2+ 100 mM used in assay conditions Klebsiella pneumoniae

Organism

EC Number Organism UniProt Comment Textmining
2.7.7.46 Klebsiella pneumoniae P0AE05
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.7.7.46
-
 Klebsiella pneumoniae

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.7.46 ATP + gentamicin
-
 Klebsiella pneumoniae diphosphate + 2''-adenylylgentamicin
-
 ?
2.7.7.46 ATP + kanamycin A
-
 Klebsiella pneumoniae diphosphate + 2''-adenylylkanamycin A
-
 ?
2.7.7.46 ATP + kanamycin B
-
 Klebsiella pneumoniae diphosphate + 2''-adenylylkanamycin B
-
 ?
2.7.7.46 ATP + tobramycin A
-
 Klebsiella pneumoniae diphosphate + 2''-adenylyltobramycin
-
 ?

Synonyms

EC Number Synonyms Comment Organism
2.7.7.46 aminoglycoside adenyltransferase 2''-Ia
-
 Klebsiella pneumoniae
2.7.7.46 ANT(2'')-Ia
-
 Klebsiella pneumoniae