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Literature summary extracted from
- Cloning, expression, identification and characterization of borneol dehydrogenase isozymes in Pseudomonas sp. TCU-HL1 (2020), Protein Expr. Purif., 175, 105715 .
Application
| EC Number | Application | Comment | Organism |
|---|---|---|---|
| 1.1.1.198 | synthesis | production of enzyme in the form of inclusion body in Escherichia coli. The refolded BDH1 tends to precipitate. Insoluble recombinant BDH1 is converted into a soluble form by adding glycerol in LB medium | Pseudomonas sp. TCU-HL1 |
| 1.1.1.227 | synthesis | production of enzyme in the form of inclusion body in Escherichia coli. The refolded BDH1 tends to precipitate. Insoluble recombinant BDH1 is converted into a soluble form by adding glycerol in LB medium | Pseudomonas sp. TCU-HL1 |
Cloned(Commentary)
| EC Number | Cloned (Comment) | Organism |
|---|---|---|
| 1.1.1.198 | expression in Escherichia coli | Pseudomonas sp. TCU-HL1 |
| 1.1.1.227 | expression in Escherichia coli | Pseudomonas sp. TCU-HL1 |
Inhibitors
| EC Number | Inhibitors | Comment | Organism | Structure |
|---|---|---|---|---|
| 1.1.1.198 | (+)-Camphor | - |
Pseudomonas sp. TCU-HL1 |  |
| 1.1.1.198 | (-)-Camphor | - |
Pseudomonas sp. TCU-HL1 | |
| 1.1.1.227 | (-)-Camphor | - |
Pseudomonas sp. TCU-HL1 | |
KM Value [mM]
| EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 1.1.1.198 | 0.07 | - |
(+)-borneol | isoform BDH2, at pH 7.0, temperature not specified in the publication | Pseudomonas sp. TCU-HL1 | |
| 1.1.1.198 | 0.09 | - |
(+)-borneol | soluble isoform BDH1, at pH 7.0, temperature not specified in the publication | Pseudomonas sp. TCU-HL1 | |
| 1.1.1.198 | 0.09 | - |
(+)-borneol | soluble recombinant protein, pH 7, 25°C | Pseudomonas sp. TCU-HL1 | |
| 1.1.1.198 | 0.12 | - |
(-)-borneol | refolded isoform BDH1, at pH 7.0, temperature not specified in the publication | Pseudomonas sp. TCU-HL1 | |
| 1.1.1.198 | 0.12 | - |
(+)-borneol | refolded isoform BDH1, at pH 7.0, temperature not specified in the publication | Pseudomonas sp. TCU-HL1 | |
| 1.1.1.198 | 0.12 | - |
(+)-borneol | refolded recombinant protein, pH 7, 25°C | Pseudomonas sp. TCU-HL1 | |
| 1.1.1.198 | 0.21 | - |
(-)-borneol | soluble isoform BDH1, at pH 7.0, temperature not specified in the publication | Pseudomonas sp. TCU-HL1 | |
| 1.1.1.198 | 1.5 | - |
(-)-borneol | isoform BDH2, at pH 7.0, temperature not specified in the publication | Pseudomonas sp. TCU-HL1 | |
| 1.1.1.227 | 0.21 | - |
(-)-borneol | soluble recombinant protein, pH 7, 25°C | Pseudomonas sp. TCU-HL1 | |
| 1.1.1.227 | 1.5 | - |
(-)-borneol | refolded recombinant protein, pH 7, 25°C | Pseudomonas sp. TCU-HL1 | |
Natural Substrates/ Products (Substrates)
| EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 1.1.1.198 | (+)-borneol + NAD+ | Pseudomonas sp. TCU-HL1 | - |
(+)-camphor + NADH + H+ | - |
? | |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 1.1.1.198 | Pseudomonas sp. TCU-HL1 | - |
- |
- |
| 1.1.1.198 | Pseudomonas sp. TCU-HL1 | A0A1B3EB36 | isoform BDH1 | - |
| 1.1.1.227 | Pseudomonas sp. TCU-HL1 | A0A1B3EB36 | isoform BDH1, cf. EC 1.1.1.198 | - |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 1.1.1.198 | ammonium sulfate precipitation, phenyl Sepharose column chromatography and Q-Sepharose column chromatography | Pseudomonas sp. TCU-HL1 |
| 1.1.1.198 | production of enzyme in the form of inclusion body in Escherichia coli. The refolded BDH1 tends to precipitate. Insoluble recombinant BDH1 is converted into a soluble form by adding glycerol in LB medium | Pseudomonas sp. TCU-HL1 |
| 1.1.1.227 | production of enzyme in the form of inclusion body in Escherichia coli. The refolded BDH1 tends to precipitate. Insoluble recombinant BDH1 is converted into a soluble form by adding glycerol in LB medium | Pseudomonas sp. TCU-HL1 |
Renatured (Commentary)
| EC Number | Renatured (Comment) | Organism |
|---|---|---|
| 1.1.1.198 | - |
Pseudomonas sp. TCU-HL1 |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 1.1.1.198 | (+)-borneol + NAD+ | - |
Pseudomonas sp. TCU-HL1 | (+)-camphor + NADH + H+ | - |
? | |
| 1.1.1.198 | (+)-borneol + NAD+ | the enzyme prefers to degrade (+)-borneol, rather than (-)-borneol | Pseudomonas sp. TCU-HL1 | (+)-camphor + NADH + H+ | - |
? | |
| 1.1.1.198 | (-)-borneol + NAD+ | - |
Pseudomonas sp. TCU-HL1 | (-)-camphor + NADH + H+ | - |
? | |
| 1.1.1.198 | additional information | enzyme additionally catalyzes the reaction with (-)-borneol, reaction of EC 1.1.1.227. (+)-Borneol is the preferred substrate | Pseudomonas sp. TCU-HL1 | ? | - |
- |
|
| 1.1.1.227 | (-)-borneol + NAD+ | - |
Pseudomonas sp. TCU-HL1 | (-)-camphor + NADH + H+ | - |
? | |
| 1.1.1.227 | additional information | (+)-borneol is the preferred substrate, reaction of EC 1.1.1.198 | Pseudomonas sp. TCU-HL1 | ? | - |
- |
|
Subunits
| EC Number | Subunits | Comment | Organism |
|---|---|---|---|
| 1.1.1.198 | ? | x * about 30000, isoform BDH1, SDS-PAGE | Pseudomonas sp. TCU-HL1 |
| 1.1.1.198 | ? | x * about 35000, isoform BDH2, SDS-PAGE | Pseudomonas sp. TCU-HL1 |
Synonyms
| EC Number | Synonyms | Comment | Organism |
|---|---|---|---|
| 1.1.1.198 | BDH1 | - |
Pseudomonas sp. TCU-HL1 |
| 1.1.1.198 | BDH1 | isoform | Pseudomonas sp. TCU-HL1 |
| 1.1.1.198 | BDH2 | isoform | Pseudomonas sp. TCU-HL1 |
| 1.1.1.198 | THL1_4180 | - |
Pseudomonas sp. TCU-HL1 |
| 1.1.1.227 | BDH1 | cf. EC 1.1.1.198 | Pseudomonas sp. TCU-HL1 |
| 1.1.1.227 | THL1_4180 | - |
Pseudomonas sp. TCU-HL1 |
Turnover Number [1/s]
| EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 1.1.1.198 | 0.38 | - |
(-)-borneol | refolded isoform BDH1, at pH 7.0, temperature not specified in the publication | Pseudomonas sp. TCU-HL1 | |
| 1.1.1.198 | 0.5 | - |
(+)-borneol | refolded isoform BDH1, at pH 7.0, temperature not specified in the publication | Pseudomonas sp. TCU-HL1 | |
| 1.1.1.198 | 0.5 | - |
(+)-borneol | refolded recombinant protein, pH 7, 25°C | Pseudomonas sp. TCU-HL1 | |
| 1.1.1.198 | 12.4 | - |
(-)-borneol | soluble isoform BDH1, at pH 7.0, temperature not specified in the publication | Pseudomonas sp. TCU-HL1 | |
| 1.1.1.198 | 17 | - |
(+)-borneol | soluble isoform BDH1, at pH 7.0, temperature not specified in the publication | Pseudomonas sp. TCU-HL1 | |
| 1.1.1.198 | 17 | - |
(+)-borneol | soluble recombinant protein, pH 7, 25°C | Pseudomonas sp. TCU-HL1 | |
| 1.1.1.198 | 18.5 | - |
(-)-borneol | isoform BDH2, at pH 7.0, temperature not specified in the publication | Pseudomonas sp. TCU-HL1 | |
| 1.1.1.198 | 27.8 | - |
(+)-borneol | isoform BDH2, at pH 7.0, temperature not specified in the publication | Pseudomonas sp. TCU-HL1 | |
| 1.1.1.227 | 0.38 | - |
(-)-borneol | refolded recombinant protein, pH 7, 25°C | Pseudomonas sp. TCU-HL1 | |
| 1.1.1.227 | 12.4 | - |
(-)-borneol | soluble recombinant protein, pH 7, 25°C | Pseudomonas sp. TCU-HL1 | |
Cofactor
| EC Number | Cofactor | Comment | Organism | Structure |
|---|---|---|---|---|
| 1.1.1.198 | NAD+ | - |
Pseudomonas sp. TCU-HL1 | |
Ki Value [mM]
| EC Number | Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 1.1.1.198 | 0.07 | - |
(+)-Camphor | soluble isoform BDH1, at pH 7.0, temperature not specified in the publication | Pseudomonas sp. TCU-HL1 | |
| 1.1.1.198 | 0.07 | - |
(+)-Camphor | soluble recombinant protein, pH 7, 25°C | Pseudomonas sp. TCU-HL1 | |
| 1.1.1.198 | 0.08 | - |
(-)-Camphor | soluble isoform BDH1, at pH 7.0, temperature not specified in the publication | Pseudomonas sp. TCU-HL1 | |
| 1.1.1.198 | 0.16 | - |
(+)-Camphor | isoform BDH2, at pH 7.0, temperature not specified in the publication | Pseudomonas sp. TCU-HL1 | |
| 1.1.1.198 | 0.16 | - |
(+)-Camphor | refolded recombinant protein, pH 7, 25°C | Pseudomonas sp. TCU-HL1 | |
| 1.1.1.198 | 0.52 | - |
(-)-Camphor | isoform BDH2, at pH 7.0, temperature not specified in the publication | Pseudomonas sp. TCU-HL1 | |
| 1.1.1.227 | 0.08 | - |
(-)-Camphor | soluble recombinant protein, pH 7, 25°C | Pseudomonas sp. TCU-HL1 | |
| 1.1.1.227 | 0.52 | - |
(-)-Camphor | refolded recombinant protein, pH 7, 25°C | Pseudomonas sp. TCU-HL1 | |
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