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Literature summary extracted from

  • Lu, H.; Bai, Y.; Fan, T.; Zhao, Y.; Zheng, X.; Cai, Y.
    Identification of a L-Lactate dehydrogenase with 3,4-dihydroxyphenylpyruvic reduction activity for L-danshensu production (2018), Process Biochem., 72, 119-123 .
No PubMed abstract available

Application

EC Number Application Comment Organism
1.1.1.27 synthesis coexpression of enzyme and glucose dehydrogenase gene in Escherichia coli efficiently reduces 3,4-dihydroxyphenylpyruvate to L-3,4-dihydroxyphenyllactate with 95.45% isolation yield Limosilactobacillus fermentum

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.1.1.27 Cu2+ 6 h, 60% decrease in activity Limosilactobacillus fermentum
1.1.1.27 Fe2+ 6 h, 70% decrease in activity Limosilactobacillus fermentum

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.1.1.27 0.123
-
NADH pH 6, 25°C Limosilactobacillus fermentum
1.1.1.27 0.197
-
NADPH pH 6, 25°C Limosilactobacillus fermentum
1.1.1.27 2.47
-
pyruvate pH 6, 25°C Limosilactobacillus fermentum
1.1.1.27 11.37
-
3,4-Dihydroxyphenylpyruvate pH 6, 25°C Limosilactobacillus fermentum

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.1.1.27 K+ 6 h, 20% increase in activity Limosilactobacillus fermentum

Organism

EC Number Organism UniProt Comment Textmining
1.1.1.27 Limosilactobacillus fermentum A0A2K9Y5T7
-
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.1.1.27 16.48
-
substrate 3,4-dihydroxyphenylpyruvate, pH 6, 25°C Limosilactobacillus fermentum
1.1.1.27 144.8
-
substrate pyruvate, pH 6, 25°C Limosilactobacillus fermentum

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.1.27 2-oxobutyrate + NADH + H+ 11.5% of the activity with pyruvate Limosilactobacillus fermentum 2-hydroxybutyrate + NAD+
-
?
1.1.1.27 2-oxoglutarate + NADH + H+ 14.6% of the activity with pyruvate Limosilactobacillus fermentum 2-hydroxyglutarate + NAD+
-
?
1.1.1.27 3,4-dihydroxyphenylpyruvate + NADH + H+ 8.5% of the activity with pyruvate Limosilactobacillus fermentum L-3,4-dihydroxyphenyllactate + NAD+
-
?
1.1.1.27 4-hydroxyphenylpyruvate + NADH + H+ 11.1% of the activity with pyruvate Limosilactobacillus fermentum L-4-hydroxyphenyllactate + NAD+
-
?
1.1.1.27 4-methyl-2-oxopentanoate + NADH + H+ 25.9% of the activity with pyruvate Limosilactobacillus fermentum 2-hydroxy-4-methylpentanoate + NAD+
-
?
1.1.1.27 benzoylformate + NADH + H+ 20.5% of the activity with pyruvate Limosilactobacillus fermentum L-hydroxy(phenyl)acetate + NAD+
-
?
1.1.1.27 phenylpyruvate + NADH + H+ 47.4% of the activity with pyruvate Limosilactobacillus fermentum L-phenyllactate + NAD+
-
?
1.1.1.27 pyruvate + NADPH + H+
-
Limosilactobacillus fermentum L-lactate + NADP+
-
?

Subunits

EC Number Subunits Comment Organism
1.1.1.27 ? x * 33650, calcuated from sequence Limosilactobacillus fermentum

Synonyms

EC Number Synonyms Comment Organism
1.1.1.27 L-Ldh0845
-
Limosilactobacillus fermentum

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.1.1.27 25
-
-
Limosilactobacillus fermentum

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.1.1.27 0.293
-
3,4-Dihydroxyphenylpyruvate pH 6, 25°C Limosilactobacillus fermentum
1.1.1.27 10.37
-
NADPH pH 6, 25°C Limosilactobacillus fermentum
1.1.1.27 12.45
-
pyruvate pH 6, 25°C Limosilactobacillus fermentum
1.1.1.27 64.42
-
NADH pH 6, 25°C Limosilactobacillus fermentum

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.1.1.27 6
-
-
Limosilactobacillus fermentum

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
1.1.1.27 6
-
stable Limosilactobacillus fermentum

Cofactor

EC Number Cofactor Comment Organism Structure
1.1.1.27 NADH NADH is 10fold preferred over NADPH Limosilactobacillus fermentum
1.1.1.27 NADPH NADH is 10fold preferred over NADPH Limosilactobacillus fermentum

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
1.1.1.27 0.0258
-
3,4-Dihydroxyphenylpyruvate pH 6, 25°C Limosilactobacillus fermentum
1.1.1.27 5.05
-
pyruvate pH 6, 25°C Limosilactobacillus fermentum
1.1.1.27 52.52
-
NADPH pH 6, 25°C Limosilactobacillus fermentum
1.1.1.27 521.9
-
NADH pH 6, 25°C Limosilactobacillus fermentum