EC Number | Application | Comment | Organism |
---|---|---|---|
1.1.1.27 | synthesis | coexpression of enzyme and glucose dehydrogenase gene in Escherichia coli efficiently reduces 3,4-dihydroxyphenylpyruvate to L-3,4-dihydroxyphenyllactate with 95.45% isolation yield | Limosilactobacillus fermentum |
EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
1.1.1.27 | Cu2+ | 6 h, 60% decrease in activity | Limosilactobacillus fermentum | |
1.1.1.27 | Fe2+ | 6 h, 70% decrease in activity | Limosilactobacillus fermentum |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
1.1.1.27 | 0.123 | - |
NADH | pH 6, 25°C | Limosilactobacillus fermentum | |
1.1.1.27 | 0.197 | - |
NADPH | pH 6, 25°C | Limosilactobacillus fermentum | |
1.1.1.27 | 2.47 | - |
pyruvate | pH 6, 25°C | Limosilactobacillus fermentum | |
1.1.1.27 | 11.37 | - |
3,4-Dihydroxyphenylpyruvate | pH 6, 25°C | Limosilactobacillus fermentum |
EC Number | Metals/Ions | Comment | Organism | Structure |
---|---|---|---|---|
1.1.1.27 | K+ | 6 h, 20% increase in activity | Limosilactobacillus fermentum |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
1.1.1.27 | Limosilactobacillus fermentum | A0A2K9Y5T7 | - |
- |
EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|---|
1.1.1.27 | 16.48 | - |
substrate 3,4-dihydroxyphenylpyruvate, pH 6, 25°C | Limosilactobacillus fermentum |
1.1.1.27 | 144.8 | - |
substrate pyruvate, pH 6, 25°C | Limosilactobacillus fermentum |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.1.1.27 | 2-oxobutyrate + NADH + H+ | 11.5% of the activity with pyruvate | Limosilactobacillus fermentum | 2-hydroxybutyrate + NAD+ | - |
? | |
1.1.1.27 | 2-oxoglutarate + NADH + H+ | 14.6% of the activity with pyruvate | Limosilactobacillus fermentum | 2-hydroxyglutarate + NAD+ | - |
? | |
1.1.1.27 | 3,4-dihydroxyphenylpyruvate + NADH + H+ | 8.5% of the activity with pyruvate | Limosilactobacillus fermentum | L-3,4-dihydroxyphenyllactate + NAD+ | - |
? | |
1.1.1.27 | 4-hydroxyphenylpyruvate + NADH + H+ | 11.1% of the activity with pyruvate | Limosilactobacillus fermentum | L-4-hydroxyphenyllactate + NAD+ | - |
? | |
1.1.1.27 | 4-methyl-2-oxopentanoate + NADH + H+ | 25.9% of the activity with pyruvate | Limosilactobacillus fermentum | 2-hydroxy-4-methylpentanoate + NAD+ | - |
? | |
1.1.1.27 | benzoylformate + NADH + H+ | 20.5% of the activity with pyruvate | Limosilactobacillus fermentum | L-hydroxy(phenyl)acetate + NAD+ | - |
? | |
1.1.1.27 | phenylpyruvate + NADH + H+ | 47.4% of the activity with pyruvate | Limosilactobacillus fermentum | L-phenyllactate + NAD+ | - |
? | |
1.1.1.27 | pyruvate + NADPH + H+ | - |
Limosilactobacillus fermentum | L-lactate + NADP+ | - |
? |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
1.1.1.27 | ? | x * 33650, calcuated from sequence | Limosilactobacillus fermentum |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
1.1.1.27 | L-Ldh0845 | - |
Limosilactobacillus fermentum |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
1.1.1.27 | 25 | - |
- |
Limosilactobacillus fermentum |
EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
1.1.1.27 | 0.293 | - |
3,4-Dihydroxyphenylpyruvate | pH 6, 25°C | Limosilactobacillus fermentum | |
1.1.1.27 | 10.37 | - |
NADPH | pH 6, 25°C | Limosilactobacillus fermentum | |
1.1.1.27 | 12.45 | - |
pyruvate | pH 6, 25°C | Limosilactobacillus fermentum | |
1.1.1.27 | 64.42 | - |
NADH | pH 6, 25°C | Limosilactobacillus fermentum |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
1.1.1.27 | 6 | - |
- |
Limosilactobacillus fermentum |
EC Number | pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|---|
1.1.1.27 | 6 | - |
stable | Limosilactobacillus fermentum |
EC Number | Cofactor | Comment | Organism | Structure |
---|---|---|---|---|
1.1.1.27 | NADH | NADH is 10fold preferred over NADPH | Limosilactobacillus fermentum | |
1.1.1.27 | NADPH | NADH is 10fold preferred over NADPH | Limosilactobacillus fermentum |
EC Number | kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
1.1.1.27 | 0.0258 | - |
3,4-Dihydroxyphenylpyruvate | pH 6, 25°C | Limosilactobacillus fermentum | |
1.1.1.27 | 5.05 | - |
pyruvate | pH 6, 25°C | Limosilactobacillus fermentum | |
1.1.1.27 | 52.52 | - |
NADPH | pH 6, 25°C | Limosilactobacillus fermentum | |
1.1.1.27 | 521.9 | - |
NADH | pH 6, 25°C | Limosilactobacillus fermentum |