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Literature summary extracted from

  • Ehmann, D.; Shaw-Reid, C.; Losey, H.; Walsh, C.
    The EntF and EntE adenylation domains of Escherichia coli enterobactin synthetase Sequestration and selectivity in acyl-AMP transfers to thiolation domain cosubstrates (2000), Proc. Natl. Acad. Sci. USA, 97, 2509-2514 .
    View publication on PubMed

Organism

EC Number Organism UniProt Comment Textmining
6.2.1.71 Escherichia coli P10378
-
-
6.2.1.72 Escherichia coli P11454
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
6.2.1.71 ATP + 2,3-dihydroxybenzoate + [aryl-carrier protein domain of EntB]
-
 Escherichia coli AMP + diphosphate + 2,3-dihydroxybenzoyl-[aryl-carrier protein domain of EntB]
-
 ?
6.2.1.71 ATP + salicylate + holo-[aryl-carrier protein domain of EntB]
-
 Escherichia coli AMP + diphosphate + salicyl-[aryl-carrier protein domain of EntB]
-
 ?
6.2.1.71 ATP + salicylate + holo-[aryl-carrier protein domain of SrfB1] SrfB1, i.e. peptidyl carrier protein of surfactin synthetase Escherichia coli AMP + diphosphate + salicyl-[aryl-carrier protein domain of SrfB1]
-
 ?
6.2.1.72 (L-seryl)adenylate + holo-[L-seryl-carrier protein]
-
 Escherichia coli AMP + L-seryl-[L-seryl-carrier protein]
-
 ?
6.2.1.72 ATP + L-serine
-
 Escherichia coli diphosphate + (L-seryl)adenylate
-
 ?
6.2.1.72 ATP + L-serine + holo-[L-seryl-carrier protein domain of EntF]
-
 Escherichia coli AMP + diphosphate + L-seryl-[L-seryl-carrier protein domain of EntF]
-
 ?
6.2.1.72 ATP + L-serine + holo-[L-seryl-carrier protein domain of SrfB1]
-
 Escherichia coli AMP + diphosphate + L-seryl-[L-seryl-carrier protein domain of SrfB1]
-
 ?
6.2.1.72 ATP + L-serine + holo-[L-seryl-carrier protein domain of Ybt]
-
 Escherichia coli AMP + diphosphate + L-seryl-[L-seryl-carrier protein domain of Ybt]
-
 ?

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
6.2.1.71 0.008
-
 salicylate acceptor aryl-carrier protein domain of SrfB1, pH 7.5, 37°C Escherichia coli
6.2.1.71 2.15
-
 salicylate acceptor aryl-carrier protein domain of EntB, pH 7.5, 37°C Escherichia coli
6.2.1.72 0.00167
-
 L-serine acceptor holo-[L-seryl-carrier protein domain of Ybt], pH 7.5, 37°C Escherichia coli
6.2.1.72 0.0083
-
 L-serine acceptor holo-[L-seryl-carrier protein domain of SrfB1], pH 7.5, 37°C Escherichia coli

General Information

EC Number General Information Comment Organism
6.2.1.71 physiological function enterobactin, the tris-(N-(2,3-dihydroxybenzoyl)serine) trilactone siderophore of Escherichia coli, is synthesized by a three-protein (EntE, B, F) six-module nonribosomal peptide synthetase Escherichia coli
6.2.1.72 physiological function 142-kDa four-domain protein EntF is bisected into two double-domain fragments: a 108-kDa condensation and adenylation construct, EntF C-A, and a 37-kDa peptidyl carrier protein (PCP) and thioesterase protein, EntF PCP-TE. The adenylation domain activity of EntF C-A forms seryl-AMP but has lost the ability to transfer the seryl moiety to the cognate EntF PCP-TE in trans. Seryl transfer to heterologous PCP protein fragments, the SrfB1 PCP from surfactin synthetase and Ybt PCP1 from yersiniabactin synthetase, is observed at rates of 0.5 per min and 0.01 per min, respectively. The holo SrfB1 PCP protein as well as Ybt PCP1 do not stimulate an increase in diphosphate release from EntF C-A or EntE Escherichia coli