Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Burke, C.R.; Luptak, A.
    DNA synthesis from diphosphate substrates by DNA polymerases (2018), Proc. Natl. Acad. Sci. USA, 115, 980-985 .
    View publication on PubMedView publication on EuropePMC

Application

EC Number Application Comment Organism
2.7.7.7 biotechnology DNA polymerase plays prominent roles in numerous biotechnologies. The use of diphosphate substrates has the potential to make practical the incorporation of expensive analogs, such as isotopically labeled or chemically modified nucleotides, eliminating the need for challenging triphosphate syntheses. This feature of DNA polymerases may also provide a method for detecting nucleotides used in high-throughput DNA sequencing Thermus aquaticus
2.7.7.7 biotechnology DNA polymerase plays prominent roles in numerous biotechnologies. The use of diphosphate substrates has the potential to make practical the incorporation of expensive analogs, such as isotopically labeled or chemically modified nucleotides, eliminating the need for challenging triphosphate syntheses. This feature of DNA polymerases may also provide a method for detecting nucleotides used in high-throughput DNA sequencing Pyrococcus furiosus
2.7.7.7 biotechnology DNA polymerase plays prominent roles in numerous biotechnologies. The use of diphosphate substrates has the potential to make practical the incorporation of expensive analogs, such as isotopically labeled or chemically modified nucleotides, eliminating the need for challenging triphosphate syntheses. This feature of DNA polymerases may also provide a method for detecting nucleotides used in high-throughput DNA sequencing Thermococcus litoralis
2.7.7.7 biotechnology DNA polymerase plays prominent roles in numerous biotechnologies. The use of diphosphate substrates has the potential to make practical the incorporation of expensive analogs, such as isotopically labeled or chemically modified nucleotides, eliminating the need for challenging triphosphate syntheses. This feature of DNA polymerases may also provide a method for detecting nucleotides used in high-throughput DNA sequencing Bacillus subtilis
2.7.7.7 biotechnology DNA polymerase plays prominent roles in numerous biotechnologies. The use of diphosphate substrates has the potential to make practical the incorporation of expensive analogs, such as isotopically labeled or chemically modified nucleotides, eliminating the need for challenging triphosphate syntheses. This feature of DNA polymerases may also provide a method for detecting nucleotides used in high-throughput DNA sequencing Geobacillus stearothermophilus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.7.7.7 a 2'-deoxyribonucleoside 5'-triphosphate + DNAn Thermus aquaticus DNA replication can be accomplished using dNDPs as substrates. In thermophiles, genome replication may be less sensitive to the energy charge of the cell than in mesophiles because thermostable polymerases can accept the diphosphorylated as well as the triphosphorylated substrates. DNA replication is thus less affected by the intracellular ATP/ADP ratio, and the relatively high efficiency with which DNA is synthesized at elevated temperatures suggests that thermophiles may be able to dispense with the triphosphorylated substrates entirely diphosphate + DNAn+1
-
 ?
2.7.7.7 a 2'-deoxyribonucleoside 5'-triphosphate + DNAn Pyrococcus furiosus DNA replication can be accomplished using dNDPs as substrates. In thermophiles, genome replication may be less sensitive to the energy charge of the cell than in mesophiles because thermostable polymerases can accept the diphosphorylated as well as the triphosphorylated substrates. DNA replication is thus less affected by the intracellular ATP/ADP ratio, and the relatively high efficiency with which DNA is synthesized at elevated temperatures suggests that thermophiles may be able to dispense with the triphosphorylated substrates entirely diphosphate + DNAn+1
-
 ?
2.7.7.7 a 2'-deoxyribonucleoside 5'-triphosphate + DNAn Thermococcus litoralis DNA replication can be accomplished using dNDPs as substrates. In thermophiles, genome replication may be less sensitive to the energy charge of the cell than in mesophiles because thermostable polymerases can accept the diphosphorylated as well as the triphosphorylated substrates. DNA replication is thus less affected by the intracellular ATP/ADP ratio, and the relatively high efficiency with which DNA is synthesized at elevated temperatures suggests that thermophiles may be able to dispense with the triphosphorylated substrates entirely diphosphate + DNAn+1
-
 ?
2.7.7.7 a 2'-deoxyribonucleoside 5'-triphosphate + DNAn Geobacillus stearothermophilus DNA replication can be accomplished using dNDPs as substrates. In thermophiles, genome replication may be less sensitive to the energy charge of the cell than in mesophiles because thermostable polymerases can accept the diphosphorylated as well as the triphosphorylated substrates. DNA replication is thus less affected by the intracellular ATP/ADP ratio, and the relatively high efficiency with which DNA is synthesized at elevated temperatures suggests that thermophiles may be able to dispense with the triphosphorylated substrates entirely diphosphate + DNAn+1
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.7.7.7 Bacillus subtilis P94544
-
-
2.7.7.7 Bacillus subtilis 168 P94544
-
-
2.7.7.7 Geobacillus stearothermophilus P52026
-
-
2.7.7.7 Pyrococcus furiosus P61875
-
-
2.7.7.7 Thermococcus litoralis P30317
-
-
2.7.7.7 Thermus aquaticus P19821
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.7.7 a 2'-deoxyribonucleoside 5'-triphosphate + DNAn
-
 Pyrococcus furiosus diphosphate + DNAn+1
-
 ?
2.7.7.7 a 2'-deoxyribonucleoside 5'-triphosphate + DNAn
-
 Thermococcus litoralis diphosphate + DNAn+1
-
 ?
2.7.7.7 a 2'-deoxyribonucleoside 5'-triphosphate + DNAn
-
 Bacillus subtilis diphosphate + DNAn+1
-
 ?
2.7.7.7 a 2'-deoxyribonucleoside 5'-triphosphate + DNAn
-
 Geobacillus stearothermophilus diphosphate + DNAn+1
-
 ?
2.7.7.7 a 2'-deoxyribonucleoside 5'-triphosphate + DNAn DNA replication can be accomplished using dNDPs as substrates. In thermophiles, genome replication may be less sensitive to the energy charge of the cell than in mesophiles because thermostable polymerases can accept the diphosphorylated as well as the triphosphorylated substrates. DNA replication is thus less affected by the intracellular ATP/ADP ratio, and the relatively high efficiency with which DNA is synthesized at elevated temperatures suggests that thermophiles may be able to dispense with the triphosphorylated substrates entirely Thermus aquaticus diphosphate + DNAn+1
-
 ?
2.7.7.7 a 2'-deoxyribonucleoside 5'-triphosphate + DNAn DNA replication can be accomplished using dNDPs as substrates. In thermophiles, genome replication may be less sensitive to the energy charge of the cell than in mesophiles because thermostable polymerases can accept the diphosphorylated as well as the triphosphorylated substrates. DNA replication is thus less affected by the intracellular ATP/ADP ratio, and the relatively high efficiency with which DNA is synthesized at elevated temperatures suggests that thermophiles may be able to dispense with the triphosphorylated substrates entirely Pyrococcus furiosus diphosphate + DNAn+1
-
 ?
2.7.7.7 a 2'-deoxyribonucleoside 5'-triphosphate + DNAn DNA replication can be accomplished using dNDPs as substrates. In thermophiles, genome replication may be less sensitive to the energy charge of the cell than in mesophiles because thermostable polymerases can accept the diphosphorylated as well as the triphosphorylated substrates. DNA replication is thus less affected by the intracellular ATP/ADP ratio, and the relatively high efficiency with which DNA is synthesized at elevated temperatures suggests that thermophiles may be able to dispense with the triphosphorylated substrates entirely Thermococcus litoralis diphosphate + DNAn+1
-
 ?
2.7.7.7 a 2'-deoxyribonucleoside 5'-triphosphate + DNAn DNA replication can be accomplished using dNDPs as substrates. In thermophiles, genome replication may be less sensitive to the energy charge of the cell than in mesophiles because thermostable polymerases can accept the diphosphorylated as well as the triphosphorylated substrates. DNA replication is thus less affected by the intracellular ATP/ADP ratio, and the relatively high efficiency with which DNA is synthesized at elevated temperatures suggests that thermophiles may be able to dispense with the triphosphorylated substrates entirely Geobacillus stearothermophilus diphosphate + DNAn+1
-
 ?
2.7.7.7 a 2'-deoxyribonucleoside 5'-triphosphate + DNAn activation energy analysis of the forward (DNA synthesis) and reverse (phosphorolysis of DNA) reactions catalyzed by the Taq DNA polymerase shows that DNA synthesis is strongly favored, allowing robust replication from low-energy substrates Thermus aquaticus diphosphate + DNAn+1
-
 ?
2.7.7.7 a 2'-deoxyribonucleoside 5'-triphosphate + DNAn
-
 Bacillus subtilis 168 diphosphate + DNAn+1
-
 ?
2.7.7.7 dADP + DNAn activation energy analysis of the forward (DNA synthesis) and reverse (phosphorolysis of DNA) reactions catalyzed by the Taq DNA polymerase shows that DNA synthesis is strongly favored, allowing robust replication from low-energy substrates Thermus aquaticus phosphate + DNAn+1
-
 ?

Synonyms

EC Number Synonyms Comment Organism
2.7.7.7 DeepVent DNA polymerase
-
 Thermococcus litoralis
2.7.7.7 Taq DNA polymerase
-
 Thermus aquaticus

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2.7.7.7 additional information
-
 additional information the average speed of utilization of dNTPs by the Taq polymerase is kcat: 47/s Thermus aquaticus
2.7.7.7 6
-
 dADP pH and temperature not specified in the publication Thermus aquaticus

General Information

EC Number General Information Comment Organism
2.7.7.7 metabolism DNA replication can be accomplished using dNDPs as substrates. In thermophiles, genome replication may be less sensitive to the energy charge of the cell than in mesophiles because thermostable polymerases can accept the diphosphorylated as well as the triphosphorylated substrates. DNA replication is thus less affected by the intracellular ATP/ADP ratio, and the relatively high efficiency with which DNA is synthesized at elevated temperatures suggests that thermophiles may be able to dispense with the triphosphorylated substrates entirely Thermus aquaticus
2.7.7.7 metabolism DNA replication can be accomplished using dNDPs as substrates. In thermophiles, genome replication may be less sensitive to the energy charge of the cell than in mesophiles because thermostable polymerases can accept the diphosphorylated as well as the triphosphorylated substrates. DNA replication is thus less affected by the intracellular ATP/ADP ratio, and the relatively high efficiency with which DNA is synthesized at elevated temperatures suggests that thermophiles may be able to dispense with the triphosphorylated substrates entirely Pyrococcus furiosus
2.7.7.7 metabolism DNA replication can be accomplished using dNDPs as substrates. In thermophiles, genome replication may be less sensitive to the energy charge of the cell than in mesophiles because thermostable polymerases can accept the diphosphorylated as well as the triphosphorylated substrates. DNA replication is thus less affected by the intracellular ATP/ADP ratio, and the relatively high efficiency with which DNA is synthesized at elevated temperatures suggests that thermophiles may be able to dispense with the triphosphorylated substrates entirely Thermococcus litoralis
2.7.7.7 metabolism DNA replication can be accomplished using dNDPs as substrates. In thermophiles, genome replication may be less sensitive to the energy charge of the cell than in mesophiles because thermostable polymerases can accept the diphosphorylated as well as the triphosphorylated substrates. DNA replication is thus less affected by the intracellular ATP/ADP ratio, and the relatively high efficiency with which DNA is synthesized at elevated temperatures suggests that thermophiles may be able to dispense with the triphosphorylated substrates entirely Geobacillus stearothermophilus

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
2.7.7.7 additional information
-
 additional information DNA synthesis from dNDPs is a little over an order of magnitude lower than from dNTPs and that Vmax/KM is about 400 times lower for dNDPs than for dNTPs Thermus aquaticus