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Literature summary extracted from

  • Tahara, K.; Nishiguchi, M.; Funke, E.; Miyazawa, S.I.; Miyama, T.; Milkowski, C.
    Dehydroquinate dehydratase/shikimate dehydrogenases involved in gallate biosynthesis of the aluminum-tolerant tree species Eucalyptus camaldulensis (2020), Planta, 253, 3 .
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.1.1.24 gene EcDQD/SDH4a, DNA and amino acid sequence determination and analysis, phylogenetic analysis, recombinant expression of GST-tagged enzyme in Escherichia coli strain BL21-CodonPlus(DE3)-RIL, quantitative real-time RT-PCR enzyme expression analysis Eucalyptus camaldulensis
1.1.1.24 gene EcDQD/SDH4b, DNA and amino acid sequence determination and analysis, phylogenetic analysis, recombinant expression of GST-tagged enzyme in Escherichia coli strain BL21-CodonPlus(DE3)-RIL, quantitative real-time RT-PCR enzyme expression analysis Eucalyptus camaldulensis
1.1.1.25 expression in Escherichia coli as recombinant N-terminal GST-tagged protein Eucalyptus camaldulensis
1.1.1.25 gene EcDQD/SDH1, DNA and amino acid sequence determination and analysis, phylogenetic analysis, recombinant expression of GST-tagged enzyme in Escherichia coli strain BL21-CodonPlus(DE3)-RIL, quantitative real-time RT-PCR enzyme expression analysis Eucalyptus camaldulensis
1.1.1.25 gene EcDQD/SDH2, DNA and amino acid sequence determination and analysis, phylogenetic analysis, recombinant expression of GST-tagged enzyme in Escherichia coli strain BL21-CodonPlus(DE3)-RIL, quantitative real-time RT-PCR enzyme expression analysis Eucalyptus camaldulensis
1.1.1.25 gene EcDQD/SDH3, DNA and amino acid sequence determination and analysis, phylogenetic analysis, recombinant expression of GST-tagged enzyme in Escherichia coli strain BL21-CodonPlus(DE3)-RIL, quantitative real-time RT-PCR enzyme expression analysis Eucalyptus camaldulensis
4.2.1.10 expression in Escherichia coli as recombinant N-terminal GST-tagged protein Eucalyptus camaldulensis

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.1.1.24 0.0537
-
NAD+ pH 9.0, 30°C, EcDQD/SDH4a, quinate oxidation Eucalyptus camaldulensis
1.1.1.24 0.102
-
NADH pH 7.5, 30°C, EcDQD/SDH4a, quinate formation Eucalyptus camaldulensis
1.1.1.24 0.164
-
L-quinate pH 9.0, 30°C, EcDQD/SDH4a, quinate oxidation Eucalyptus camaldulensis
1.1.1.24 1.4
-
3-dehydroquinate pH 7.5, 30°C, EcDQD/SDH4a, quinate formation Eucalyptus camaldulensis
1.1.1.25 0.00438
-
NADPH pH 8.5, temperature not specified in the publication Eucalyptus camaldulensis
1.1.1.25 0.00438
-
NADPH pH 8.5, 30°C, EcDQD/SDH2, shikimate formation Eucalyptus camaldulensis
1.1.1.25 0.0231
-
NADPH pH 6.5, 30°C, EcDQD/SDH3, shikimate formation Eucalyptus camaldulensis
1.1.1.25 0.0326
-
shikimate pH 9.0, temperature not specified in the publication Eucalyptus camaldulensis
1.1.1.25 0.0326
-
shikimate pH 9.0, 30°C, EcDQD/SDH1, shikimate oxidation Eucalyptus camaldulensis
1.1.1.25 0.0415
-
NADP+ pH 9.0, temperature not specified in the publication Eucalyptus camaldulensis
1.1.1.25 0.0415
-
NADP+ pH 9.0, 30°C, EcDQD/SDH3, shikimate oxidation Eucalyptus camaldulensis
1.1.1.25 0.0495
-
NADPH pH 6.5, temperature not specified in the publication Eucalyptus camaldulensis
1.1.1.25 0.0495
-
NADPH pH 6.5, 30°C, EcDQD/SDH1, shikimate formation Eucalyptus camaldulensis
1.1.1.25 0.0565
-
NADP+ pH 9.0, temperature not specified in the publication Eucalyptus camaldulensis
1.1.1.25 0.0565
-
NADP+ pH 9.0, 30°C, EcDQD/SDH1, shikimate oxidation Eucalyptus camaldulensis
1.1.1.25 0.0652
-
NADP+ pH 9.0, temperature not specified in the publication Eucalyptus camaldulensis
1.1.1.25 0.0652
-
NADP+ pH 9.0, 30°C, EcDQD/SDH2, shikimate oxidation Eucalyptus camaldulensis
1.1.1.25 0.322
-
3-dehydroshikimate pH 7.0, 30°C, EcDQD/SDH2, DQD activity Eucalyptus camaldulensis
1.1.1.25 0.381
-
3-dehydroshikimate pH 6.5, temperature not specified in the publication Eucalyptus camaldulensis
1.1.1.25 0.381
-
3-dehydroshikimate pH 6.5, 30°C, EcDQD/SDH1, shikimate formation Eucalyptus camaldulensis
1.1.1.25 0.449
-
3-dehydroshikimate pH 7.0, 30°C, EcDQD/SDH1, DQD activity Eucalyptus camaldulensis
1.1.1.25 0.878
-
3-dehydroshikimate pH 6.5, temperature not specified in the publication Eucalyptus camaldulensis
1.1.1.25 0.878
-
3-dehydroshikimate pH 6.5, 30°C, EcDQD/SDH3, shikimate formation Eucalyptus camaldulensis
1.1.1.25 0.882
-
3-dehydroshikimate pH 7.0, 30°C, EcDQD/SDH3, DQD activity Eucalyptus camaldulensis
1.1.1.25 2.05
-
shikimate pH 9.0, temperature not specified in the publication Eucalyptus camaldulensis
1.1.1.25 2.05
-
shikimate pH 9.0, 30°C, EcDQD/SDH2, shikimate oxidation Eucalyptus camaldulensis
1.1.1.25 3.64
-
shikimate pH 9.0, temperature not specified in the publication Eucalyptus camaldulensis
1.1.1.25 3.64
-
shikimate pH 9.0, 30°C, EcDQD/SDH3, shikimate oxidation Eucalyptus camaldulensis
1.1.1.25 8.83
-
3-dehydroshikimate pH 8.5, temperature not specified in the publication Eucalyptus camaldulensis
1.1.1.25 8.83
-
3-dehydroshikimate pH 8.5, 30°C, EcDQD/SDH2, shikimate formation Eucalyptus camaldulensis
1.1.1.25 9.44
-
3-dehydroshikimate pH 10.5, 30°C, EcDQD/SDH2, gallate formation Eucalyptus camaldulensis
1.1.1.25 23.1
-
NADPH pH 6.5, temperature not specified in the publication Eucalyptus camaldulensis
1.1.1.25 30.6
-
3-dehydroshikimate pH 10.5, 30°C, EcDQD/SDH2, gallate formation Eucalyptus camaldulensis

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.1.1.24 L-quinate + NAD+ Eucalyptus camaldulensis
-
3-dehydroquinate + NADH + H+
-
r
1.1.1.25 3-dehydroshikimate + NADP+ Eucalyptus camaldulensis
-
gallate + NADPH + H+
-
r
1.1.1.25 shikimate + NADP+ Eucalyptus camaldulensis
-
3-dehydroshikimate + NADPH + H+
-
r

Organism

EC Number Organism UniProt Comment Textmining
1.1.1.24 Eucalyptus camaldulensis A0A5H2WXM2
-
-
1.1.1.24 Eucalyptus camaldulensis A0A5H2X0F8
-
-
1.1.1.25 Eucalyptus camaldulensis A0A5H2WVH6
-
-
1.1.1.25 Eucalyptus camaldulensis A0A5H2WZU5
-
-
1.1.1.25 Eucalyptus camaldulensis A0A5H2X4C4
-
-
4.2.1.10 Eucalyptus camaldulensis A0A5H2WZU5 cf. EC 1.1.1.25
-
4.2.1.10 Eucalyptus camaldulensis A0A5H2X4C4 cf. EC 1.1.1.25
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.1.1.24 recombinant GST-tagged enzyme from Escherichia coli strain BL21-CodonPlus(DE3)-RIL by glutathione affinity chromatography and tag cleavage through thrombin Eucalyptus camaldulensis
1.1.1.25
-
Eucalyptus camaldulensis
1.1.1.25 recombinant GST-tagged enzyme from Escherichia coli strain BL21-CodonPlus(DE3)-RIL by glutathione affinity chromatography and tag cleavage through thrombin Eucalyptus camaldulensis
4.2.1.10
-
Eucalyptus camaldulensis

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.1.1.24 leaf highest expression level Eucalyptus camaldulensis
-
1.1.1.24 additional information comparison of EcDQD/SDH, UGT84A25a/b, and UGT84A26a/b expression patterns in Eucalyptus camaldulensis, relative EcDQD/SDH mRNA levels in the leaves, stems, and roots are plotted against the relative UGT84A25a/b and UGT84A26a/b mRNA levels in the same samples, and determination of concentrations of the metabolites in the different tissues, overview Eucalyptus camaldulensis
-
1.1.1.24 root
-
Eucalyptus camaldulensis
-
1.1.1.24 stem lowest expression level Eucalyptus camaldulensis
-
1.1.1.25 leaf
-
Eucalyptus camaldulensis
-
1.1.1.25 leaf highest expression level Eucalyptus camaldulensis
-
1.1.1.25 additional information comparison of EcDQD/SDH, UGT84A25a/b, and UGT84A26a/b expression patterns in Eucalyptus camaldulensis, relative EcDQD/SDH mRNA levels in the leaves, stems, and roots are plotted against the relative UGT84A25a/b and UGT84A26a/b mRNA levels in the same samples, and determination of concentrations of the metabolites in the different tissues, overview Eucalyptus camaldulensis
-
1.1.1.25 root
-
Eucalyptus camaldulensis
-
1.1.1.25 root lowest expression level Eucalyptus camaldulensis
-
1.1.1.25 stem highest expression level Eucalyptus camaldulensis
-
1.1.1.25 stem lowest expression level Eucalyptus camaldulensis
-
4.2.1.10 root
-
Eucalyptus camaldulensis
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.1.24 L-quinate + NAD+
-
Eucalyptus camaldulensis 3-dehydroquinate + NADH + H+
-
r
1.1.1.24 L-quinate + NADP+ low activity Eucalyptus camaldulensis 3-dehydroquinate + NADPH + H+
-
r
1.1.1.24 additional information product analysis by GC-MS Eucalyptus camaldulensis ?
-
-
1.1.1.25 3-dehydroshikimate + NADP+
-
Eucalyptus camaldulensis gallate + NADPH + H+
-
r
1.1.1.25 3-dehydroshikimate + NADPH + H+
-
Eucalyptus camaldulensis shikimate + NADP+
-
r
1.1.1.25 additional information product analysis by GC-MS. No or poor quinate dehydrogenase activity Eucalyptus camaldulensis ?
-
-
1.1.1.25 shikimate + NADP+
-
Eucalyptus camaldulensis 3-dehydroshikimate + NADPH + H+
-
r
4.2.1.10 3-dehydroquinate
-
Eucalyptus camaldulensis 3-dehydroshikimate + H2O
-
?

Synonyms

EC Number Synonyms Comment Organism
1.1.1.24 QDH
-
Eucalyptus camaldulensis
1.1.1.24 quinate dehydrogenase
-
Eucalyptus camaldulensis
1.1.1.25 dehydroquinate dehydratase/shikimate dehydrogenase
-
Eucalyptus camaldulensis
1.1.1.25 EcDQD
-
Eucalyptus camaldulensis
1.1.1.25 EcDQD/SDH
-
Eucalyptus camaldulensis
1.1.1.25 EcDQD/SDH1
-
Eucalyptus camaldulensis
1.1.1.25 EcDQD/SDH2
-
Eucalyptus camaldulensis
1.1.1.25 EcDQD/SDH3
-
Eucalyptus camaldulensis
1.1.1.25 More cf. EC 4.2.1.10 Eucalyptus camaldulensis
4.2.1.10 EcDQD/SDH1
-
Eucalyptus camaldulensis
4.2.1.10 EcDQD/SDH2
-
Eucalyptus camaldulensis

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.1.1.24 30
-
assay at Eucalyptus camaldulensis
1.1.1.25 30
-
assay at Eucalyptus camaldulensis

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.1.1.24 127
-
L-quinate pH 9.0, 30°C, EcDQD/SDH4a, quinate oxidation Eucalyptus camaldulensis
1.1.1.24 139
-
NAD+ pH 9.0, 30°C, EcDQD/SDH4a, quinate oxidation Eucalyptus camaldulensis
1.1.1.24 281
-
3-dehydroquinate pH 7.5, 30°C, EcDQD/SDH4a, quinate formation Eucalyptus camaldulensis
1.1.1.24 298
-
NADH pH 7.5, 30°C, EcDQD/SDH4a, quinate formation Eucalyptus camaldulensis
1.1.1.25 2.14
-
3-dehydroshikimate pH 10.5, 30°C, EcDQD/SDH2, gallate formation Eucalyptus camaldulensis
1.1.1.25 2.87
-
3-dehydroshikimate pH 10.5, 30°C, EcDQD/SDH2, gallate formation Eucalyptus camaldulensis
1.1.1.25 3.67
-
3-dehydroshikimate pH 7.0, 30°C, EcDQD/SDH1, DQD activity Eucalyptus camaldulensis
1.1.1.25 27.6
-
NADPH pH 8.5, temperature not specified in the publication Eucalyptus camaldulensis
1.1.1.25 27.6
-
NADPH pH 8.5, 30°C, EcDQD/SDH1, shikimate formation Eucalyptus camaldulensis
1.1.1.25 30.9
-
3-dehydroshikimate pH 7.0, 30°C, EcDQD/SDH1, DQD activity Eucalyptus camaldulensis
1.1.1.25 47
-
3-dehydroshikimate pH 8.5, temperature not specified in the publication Eucalyptus camaldulensis
1.1.1.25 47
-
3-dehydroshikimate pH 8.5, 30°C, EcDQD/SDH1, shikimate formation Eucalyptus camaldulensis
1.1.1.25 57.6
-
3-dehydroshikimate pH 6.5, temperature not specified in the publication Eucalyptus camaldulensis
1.1.1.25 57.6
-
3-dehydroshikimate pH 6.5, 30°C, EcDQD/SDH1, shikimate formation Eucalyptus camaldulensis
1.1.1.25 60.4
-
3-dehydroshikimate pH 7.0, 30°C, EcDQD/SDH1, DQD activity Eucalyptus camaldulensis
1.1.1.25 70.2
-
NADP+ pH 9.0, temperature not specified in the publication Eucalyptus camaldulensis
1.1.1.25 70.2
-
NADP+ pH 9.0, 30°C, EcDQD/SDH1, shikimate oxidation Eucalyptus camaldulensis
1.1.1.25 74.1
-
NADPH pH 6.5, temperature not specified in the publication Eucalyptus camaldulensis
1.1.1.25 74.1
-
NADPH pH 6.5, 30°C, EcDQD/SDH1, shikimate formation Eucalyptus camaldulensis
1.1.1.25 80.7
-
shikimate pH 9.0, temperature not specified in the publication Eucalyptus camaldulensis
1.1.1.25 80.7
-
shikimate pH 9.0, 30°C, EcDQD/SDH1, shikimate oxidation Eucalyptus camaldulensis
1.1.1.25 135
-
NADP+ pH 9.0, temperature not specified in the publication Eucalyptus camaldulensis
1.1.1.25 135
-
NADP+ pH 9.0, 30°C, EcDQD/SDH1, shikimate oxidation Eucalyptus camaldulensis
1.1.1.25 146
-
shikimate pH 9.0, temperature not specified in the publication Eucalyptus camaldulensis
1.1.1.25 146
-
shikimate pH 9.0, 30°C, EcDQD/SDH1, shikimate oxidation Eucalyptus camaldulensis
1.1.1.25 307
-
3-dehydroshikimate pH 6.5, temperature not specified in the publication Eucalyptus camaldulensis
1.1.1.25 307
-
3-dehydroshikimate pH 6.5, 30°C, EcDQD/SDH1, shikimate formation Eucalyptus camaldulensis
1.1.1.25 389
-
NADPH pH 6.5, temperature not specified in the publication Eucalyptus camaldulensis
1.1.1.25 389
-
NADPH pH 6.5, 30°C, EcDQD/SDH1, shikimate formation Eucalyptus camaldulensis
1.1.1.25 755
-
shikimate pH 9.0, temperature not specified in the publication Eucalyptus camaldulensis
1.1.1.25 755
-
shikimate pH 9.0, 30°C, EcDQD/SDH1, shikimate oxidation Eucalyptus camaldulensis
1.1.1.25 792
-
NADP+ pH 9.0, temperature not specified in the publication Eucalyptus camaldulensis
1.1.1.25 792
-
NADP+ pH 9.0, 30°C, EcDQD/SDH1, shikimate oxidation Eucalyptus camaldulensis

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.1.1.24 7.5
-
quinate formation activity Eucalyptus camaldulensis
1.1.1.24 9
-
quinate oxidation Eucalyptus camaldulensis
1.1.1.25 6.5
-
shikimate formation activity Eucalyptus camaldulensis
1.1.1.25 7
-
DQD activity Eucalyptus camaldulensis
1.1.1.25 7.5
-
quinate formation activity Eucalyptus camaldulensis
1.1.1.25 8.5
-
shikimate formation activity Eucalyptus camaldulensis
1.1.1.25 9
-
shikimate and quinate oxidation Eucalyptus camaldulensis
1.1.1.25 10.5
-
gallate formation activity Eucalyptus camaldulensis

Cofactor

EC Number Cofactor Comment Organism Structure
1.1.1.24 NAD+
-
Eucalyptus camaldulensis
1.1.1.24 NADH
-
Eucalyptus camaldulensis
1.1.1.25 NADP+
-
Eucalyptus camaldulensis
1.1.1.25 NADP+ the enzyme shows a clear preference for NADPH/NADP+ over NADH/ NAD+ Eucalyptus camaldulensis
1.1.1.25 NADPH
-
Eucalyptus camaldulensis
1.1.1.25 NADPH the enzyme shows a clear preference for NADPH/NADP+ over NADH/ NAD+ Eucalyptus camaldulensis

General Information

EC Number General Information Comment Organism
1.1.1.24 evolution plant SDH enzymes are fused to dehydroquinate dehydratases (DQDs, EC 4.2.1.10) to form bifunctional DQD/SDH enzymes. The DQD activity is observed for EcDQD/SDH1, 2, and 3, but not for EcDQD/SDH4a. Among the active enzymes, EcDQD/SDH1 exhibits the highest DQD activity, followed by EcDQD/SDH2 (about 50% of the EcDQD/SDH1 activity) and EcDQD/SDH3 (about 5% of the EcDQD/SDH1 activity). For shikimate formation from 3-DHS as well as shikimate oxidation to 3-DHS, measurable catalytic activities are detected for EcDQD/SDH1-3, but the activities of EcDQD/SDH2 and 3 are less than 20% of those of EcDQD/SDH1. Regarding the cofactor, EcDQD/SDH1-3 have a clear preference for NADPH/NADP+ over NADH/ NAD+. In contrast, EcDQD/SDH4a and b lack shikimate formation activity. For the reverse reaction, the conversion of shikimate to 3-DHS, EcDQD/SDH4a and b display low enzymatic activity with a preference for NAD+ as the cofactor. Both EcDQD/SDH2 and 3 exhibit relatively high gallate formation activity, in contrast to the low activity of EcDQD/SDH1. The preferred cofactor in this reaction is NADP+. The reversible quinate formation from 3-DHQ is catalyzed only by EcDQD/SDH4a/b, with NADH/NAD+ as the preferred cofactor. The reaction specificity of EcDQD/SDH4a confirms the sequence-based prediction that EcDQD/SDH4a is a functional QDH enzyme. This enzyme should be renamed EcQDHa and its closest relative, EcDQD/SDH4b, should be renamed EcQDHb. The EcDQD/SDH4a and EcDQD/SDH4b genes may represent allelic variants encoding enzymes with 99.2% amino acid identity Eucalyptus camaldulensis
1.1.1.25 evolution plant SDH enzymes are fused to dehydroquinate dehydratases (DQDs, EC 4.2.1.10) to form bifunctional DQD/SDH enzymes. The DQD activity is observed for EcDQD/SDH1, 2, and 3, but not for EcDQD/SDH4a. Among the active enzymes, EcDQD/SDH1 exhibits the highest DQD activity, followed by EcDQD/SDH2 (about 50% of the EcDQD/SDH1 activity) and EcDQD/SDH3 (about 5% of the EcDQD/SDH1 activity). For shikimate formation from 3-DHS as well as shikimate oxidation to 3-DHS, measurable catalytic activities are detected for EcDQD/SDH1-3, but the activities of EcDQD/SDH2 and 3 are less than 20% of those of EcDQD/SDH1. Regarding the cofactor, EcDQD/SDH1-3 have a clear preference for NADPH/NADP+ over NADH/ NAD+. In contrast, EcDQD/SDH4a and b lack shikimate formation activity. For the reverse reaction, the conversion of shikimate to 3-DHS, EcDQD/SDH4a and b display low enzymatic activity with a preference for NAD+ as the cofactor. Both EcDQD/SDH2 and 3 exhibit relatively high gallate formation activity, in contrast to the low activity of EcDQD/SDH1. The preferred cofactor in this reaction is NADP+ Eucalyptus camaldulensis
1.1.1.25 metabolism shikimate, quinate, and gallate biosynthesis catalyzed by DQD/SDH family enzymes, overview. Plant DQD/SDHs are proposed to link the shikimate pathway to gallate and quinate metabolism Eucalyptus camaldulensis
1.1.1.25 metabolism shikimate, quinate, and gallate biosynthesis catalyzed by DQD/SDH family enzymes, overview. Plant DQD/SDHs are proposed to link the shikimate pathway to gallate and quinate metabolism. Isozymes EcDQD/SDH2 and 3 from Eucalyptus camaldulensis catalyze the NADP+-dependent oxidation of 3-dehydroshikimate to produce gallate. In Eucalyptus camaldulensis, EcDQD/SDH2 and 3 are co-expressed with UGT84A25a/b and UGT84A26a/b involved in hydrolyzable tannin biosynthesis, catalyze the synthesis of beta-glucogallin Eucalyptus camaldulensis
1.1.1.25 metabolism shikimate, quinate, and gallate biosynthesis catalyzed by DQD/SDH family enzymes, overview. Plant DQD/SDHs are proposed to link the shikimate pathway to gallate and quinate metabolism. Isozymes EcDQD/SDH2 and 3 from Eucalyptus camaldulensis catalyze the NADP+-dependent oxidation of 3-dehydroshikimate to produce gallate. In Eucalyptus camaldulensis, EcDQD/SDH2 and 3 are co-expressed with UGT84A25a/b and UGT84A26a/b involved in hydrolyzable tannin biosynthesis, they catalyze the synthesis of beta-glucogallin Eucalyptus camaldulensis
1.1.1.25 additional information EcDQD/SDH1 enzyme structure homology modeling using the structure of Arabidopsis thaliana AtDQD/SDH (Uniprot ID Q9SQT8) as template, docking of 3-dehydroshikimate into the active site of the models as well as the crystal structure of PDB ID 2O7S, molecular dynamics simulation of protein-ligand interaction Eucalyptus camaldulensis
1.1.1.25 additional information EcDQD/SDH2 enzyme structure homology modeling using the structure of Arabidopsis thaliana AtDQD/SDH (Uniprot ID Q9SQT8) as template, docking of 3-dehydroshikimate into the active site of the models as well as the crystal structure of PDB ID 2O7S, molecular dynamics simulation of protein-ligand interaction Eucalyptus camaldulensis
1.1.1.25 additional information EcDQD/SDH3 enzyme structure homology modeling using the structure of Arabidopsis thaliana AtDQD/SDH (Uniprot ID Q9SQT8) as template, docking of 3-dehydroshikimate into the active site of the models as well as the crystal structure of PDB ID 2O7S, molecular dynamics simulation of protein-ligand interaction Eucalyptus camaldulensis
1.1.1.25 physiological function the tree species Eucalyptus camaldulensis shows exceptionally high tolerance against aluminum, a widespread toxic metal in acidic soils. In the roots of Eucalyptus camaldulensis, aluminum is detoxified via the complexation with oenothein B, a hydrolyzable tannin. The biosynthesis of oenothein B involves dehydroquinate dehydratase/shikimate dehydrogenases (EcDQD/SDHs) which catalyzes the formation of gallate, the phenolic constituent of hydrolyzable tannins Eucalyptus camaldulensis
1.1.1.25 physiological function the tree species Eucalyptus camaldulensis shows exceptionally high tolerance against aluminum, a widespread toxic metal in acidic soils. In the roots of Eucalyptus camaldulensis, aluminum is detoxified via the complexation with oenothein B, a hydrolyzable tannin. The biosynthesis of oenothein B involves dehydroquinate dehydratase/shikimate dehydrogenases (EcDQD/SDHs) which catalyzes the formation of gallate, the phenolic constituent of hydrolyzable tannins. Two enzymes, EcDQD/SDH2 and 3, in Eucalyptus camaldulensis catalyze the NADP+-dependent oxidation of 3-dehydroshikimate to produce gallate. EcDQD/SDH2 and 3 maintain DQD and SDH activities, resulting in a 3-dehydroshikimate supply for gallate formation Eucalyptus camaldulensis

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
1.1.1.24 200.7
-
3-dehydroquinate pH 7.5, 30°C, EcDQD/SDH4a, quinate formation Eucalyptus camaldulensis
1.1.1.24 774.4
-
L-quinate pH 9.0, 30°C, EcDQD/SDH4a, quinate oxidation Eucalyptus camaldulensis
1.1.1.24 2588.5
-
NAD+ pH 9.0, 30°C, EcDQD/SDH4a, quinate oxidation Eucalyptus camaldulensis
1.1.1.24 2921.6
-
NADH pH 7.5, 30°C, EcDQD/SDH4a, quinate formation Eucalyptus camaldulensis
1.1.1.25 0.07
-
3-dehydroshikimate pH 10.5, 30°C, EcDQD/SDH2, gallate formation Eucalyptus camaldulensis
1.1.1.25 0.304
-
3-dehydroshikimate pH 10.5, 30°C, EcDQD/SDH2, gallate formation Eucalyptus camaldulensis
1.1.1.25 4.16
-
3-dehydroshikimate pH 7.0, 30°C, EcDQD/SDH3, DQD activity Eucalyptus camaldulensis
1.1.1.25 5.32
-
3-dehydroshikimate pH 8.5, temperature not specified in the publication Eucalyptus camaldulensis
1.1.1.25 5.32
-
3-dehydroshikimate pH 8.5, 30°C, EcDQD/SDH2, shikimate formation Eucalyptus camaldulensis
1.1.1.25 22.17
-
shikimate pH 9.0, 30°C, EcDQD/SDH3, shikimate oxidation Eucalyptus camaldulensis
1.1.1.25 22.2
-
shikimate pH 9.0, temperature not specified in the publication Eucalyptus camaldulensis
1.1.1.25 65.6
-
3-dehydroshikimate pH 6.5, 30°C, EcDQD/SDH3, shikimate formation Eucalyptus camaldulensis
1.1.1.25 65.6
-
3-dehydroshikimate pH 6.5, temperature not specified in the publication Eucalyptus camaldulensis
1.1.1.25 71.2
-
shikimate pH 9.0, 30°C, EcDQD/SDH2, shikimate oxidation Eucalyptus camaldulensis
1.1.1.25 71.3
-
shikimate pH 9.0, temperature not specified in the publication Eucalyptus camaldulensis
1.1.1.25 96
-
3-dehydroshikimate pH 7.0, 30°C, EcDQD/SDH2, DQD activity Eucalyptus camaldulensis
1.1.1.25 134.5
-
3-dehydroshikimate pH 7.0, 30°C, EcDQD/SDH1, DQD activity Eucalyptus camaldulensis
1.1.1.25 805
-
3-dehydroshikimate pH 6.5, temperature not specified in the publication Eucalyptus camaldulensis
1.1.1.25 805.8
-
3-dehydroshikimate pH 6.5, 30°C, EcDQD/SDH1, shikimate formation Eucalyptus camaldulensis
1.1.1.25 1690
-
NADP+ pH 9.0, temperature not specified in the publication Eucalyptus camaldulensis
1.1.1.25 1691.6
-
NADP+ pH 9.0, 30°C, EcDQD/SDH3, shikimate oxidation Eucalyptus camaldulensis
1.1.1.25 2070
-
NADP+ pH 9.0, temperature not specified in the publication Eucalyptus camaldulensis
1.1.1.25 2070.6
-
NADP+ pH 9.0, 30°C, EcDQD/SDH2, shikimate oxidation Eucalyptus camaldulensis
1.1.1.25 3207.8
-
NADPH pH 6.5, 30°C, EcDQD/SDH3, shikimate formation Eucalyptus camaldulensis
1.1.1.25 3210
-
NADPH pH 6.5, temperature not specified in the publication Eucalyptus camaldulensis
1.1.1.25 6300
-
NADPH pH 8.5, temperature not specified in the publication Eucalyptus camaldulensis
1.1.1.25 6301.4
-
NADPH pH 8.5, 30°C, EcDQD/SDH2, shikimate formation Eucalyptus camaldulensis
1.1.1.25 7858.6
-
NADPH pH 6.5, 30°C, EcDQD/SDH1, shikimate formation Eucalyptus camaldulensis
1.1.1.25 7860
-
NADPH pH 6.5, temperature not specified in the publication Eucalyptus camaldulensis
1.1.1.25 14000
-
NADP+ pH 9.0, temperature not specified in the publication Eucalyptus camaldulensis
1.1.1.25 14017.7
-
NADP+ pH 9.0, 30°C, EcDQD/SDH1, shikimate oxidation Eucalyptus camaldulensis
1.1.1.25 23159.5
-
shikimate pH 9.0, 30°C, EcDQD/SDH1, shikimate oxidation Eucalyptus camaldulensis
1.1.1.25 23200
-
shikimate pH 9.0, temperature not specified in the publication Eucalyptus camaldulensis