EC Number | Cloned (Comment) | Organism |
---|---|---|
1.1.1.24 | gene EcDQD/SDH4a, DNA and amino acid sequence determination and analysis, phylogenetic analysis, recombinant expression of GST-tagged enzyme in Escherichia coli strain BL21-CodonPlus(DE3)-RIL, quantitative real-time RT-PCR enzyme expression analysis | Eucalyptus camaldulensis |
1.1.1.24 | gene EcDQD/SDH4b, DNA and amino acid sequence determination and analysis, phylogenetic analysis, recombinant expression of GST-tagged enzyme in Escherichia coli strain BL21-CodonPlus(DE3)-RIL, quantitative real-time RT-PCR enzyme expression analysis | Eucalyptus camaldulensis |
1.1.1.25 | expression in Escherichia coli as recombinant N-terminal GST-tagged protein | Eucalyptus camaldulensis |
1.1.1.25 | gene EcDQD/SDH1, DNA and amino acid sequence determination and analysis, phylogenetic analysis, recombinant expression of GST-tagged enzyme in Escherichia coli strain BL21-CodonPlus(DE3)-RIL, quantitative real-time RT-PCR enzyme expression analysis | Eucalyptus camaldulensis |
1.1.1.25 | gene EcDQD/SDH2, DNA and amino acid sequence determination and analysis, phylogenetic analysis, recombinant expression of GST-tagged enzyme in Escherichia coli strain BL21-CodonPlus(DE3)-RIL, quantitative real-time RT-PCR enzyme expression analysis | Eucalyptus camaldulensis |
1.1.1.25 | gene EcDQD/SDH3, DNA and amino acid sequence determination and analysis, phylogenetic analysis, recombinant expression of GST-tagged enzyme in Escherichia coli strain BL21-CodonPlus(DE3)-RIL, quantitative real-time RT-PCR enzyme expression analysis | Eucalyptus camaldulensis |
4.2.1.10 | expression in Escherichia coli as recombinant N-terminal GST-tagged protein | Eucalyptus camaldulensis |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
1.1.1.24 | 0.0537 | - |
NAD+ | pH 9.0, 30°C, EcDQD/SDH4a, quinate oxidation | Eucalyptus camaldulensis | |
1.1.1.24 | 0.102 | - |
NADH | pH 7.5, 30°C, EcDQD/SDH4a, quinate formation | Eucalyptus camaldulensis | |
1.1.1.24 | 0.164 | - |
L-quinate | pH 9.0, 30°C, EcDQD/SDH4a, quinate oxidation | Eucalyptus camaldulensis | |
1.1.1.24 | 1.4 | - |
3-dehydroquinate | pH 7.5, 30°C, EcDQD/SDH4a, quinate formation | Eucalyptus camaldulensis | |
1.1.1.25 | 0.00438 | - |
NADPH | pH 8.5, temperature not specified in the publication | Eucalyptus camaldulensis | |
1.1.1.25 | 0.00438 | - |
NADPH | pH 8.5, 30°C, EcDQD/SDH2, shikimate formation | Eucalyptus camaldulensis | |
1.1.1.25 | 0.0231 | - |
NADPH | pH 6.5, 30°C, EcDQD/SDH3, shikimate formation | Eucalyptus camaldulensis | |
1.1.1.25 | 0.0326 | - |
shikimate | pH 9.0, temperature not specified in the publication | Eucalyptus camaldulensis | |
1.1.1.25 | 0.0326 | - |
shikimate | pH 9.0, 30°C, EcDQD/SDH1, shikimate oxidation | Eucalyptus camaldulensis | |
1.1.1.25 | 0.0415 | - |
NADP+ | pH 9.0, temperature not specified in the publication | Eucalyptus camaldulensis | |
1.1.1.25 | 0.0415 | - |
NADP+ | pH 9.0, 30°C, EcDQD/SDH3, shikimate oxidation | Eucalyptus camaldulensis | |
1.1.1.25 | 0.0495 | - |
NADPH | pH 6.5, temperature not specified in the publication | Eucalyptus camaldulensis | |
1.1.1.25 | 0.0495 | - |
NADPH | pH 6.5, 30°C, EcDQD/SDH1, shikimate formation | Eucalyptus camaldulensis | |
1.1.1.25 | 0.0565 | - |
NADP+ | pH 9.0, temperature not specified in the publication | Eucalyptus camaldulensis | |
1.1.1.25 | 0.0565 | - |
NADP+ | pH 9.0, 30°C, EcDQD/SDH1, shikimate oxidation | Eucalyptus camaldulensis | |
1.1.1.25 | 0.0652 | - |
NADP+ | pH 9.0, temperature not specified in the publication | Eucalyptus camaldulensis | |
1.1.1.25 | 0.0652 | - |
NADP+ | pH 9.0, 30°C, EcDQD/SDH2, shikimate oxidation | Eucalyptus camaldulensis | |
1.1.1.25 | 0.322 | - |
3-dehydroshikimate | pH 7.0, 30°C, EcDQD/SDH2, DQD activity | Eucalyptus camaldulensis | |
1.1.1.25 | 0.381 | - |
3-dehydroshikimate | pH 6.5, temperature not specified in the publication | Eucalyptus camaldulensis | |
1.1.1.25 | 0.381 | - |
3-dehydroshikimate | pH 6.5, 30°C, EcDQD/SDH1, shikimate formation | Eucalyptus camaldulensis | |
1.1.1.25 | 0.449 | - |
3-dehydroshikimate | pH 7.0, 30°C, EcDQD/SDH1, DQD activity | Eucalyptus camaldulensis | |
1.1.1.25 | 0.878 | - |
3-dehydroshikimate | pH 6.5, temperature not specified in the publication | Eucalyptus camaldulensis | |
1.1.1.25 | 0.878 | - |
3-dehydroshikimate | pH 6.5, 30°C, EcDQD/SDH3, shikimate formation | Eucalyptus camaldulensis | |
1.1.1.25 | 0.882 | - |
3-dehydroshikimate | pH 7.0, 30°C, EcDQD/SDH3, DQD activity | Eucalyptus camaldulensis | |
1.1.1.25 | 2.05 | - |
shikimate | pH 9.0, temperature not specified in the publication | Eucalyptus camaldulensis | |
1.1.1.25 | 2.05 | - |
shikimate | pH 9.0, 30°C, EcDQD/SDH2, shikimate oxidation | Eucalyptus camaldulensis | |
1.1.1.25 | 3.64 | - |
shikimate | pH 9.0, temperature not specified in the publication | Eucalyptus camaldulensis | |
1.1.1.25 | 3.64 | - |
shikimate | pH 9.0, 30°C, EcDQD/SDH3, shikimate oxidation | Eucalyptus camaldulensis | |
1.1.1.25 | 8.83 | - |
3-dehydroshikimate | pH 8.5, temperature not specified in the publication | Eucalyptus camaldulensis | |
1.1.1.25 | 8.83 | - |
3-dehydroshikimate | pH 8.5, 30°C, EcDQD/SDH2, shikimate formation | Eucalyptus camaldulensis | |
1.1.1.25 | 9.44 | - |
3-dehydroshikimate | pH 10.5, 30°C, EcDQD/SDH2, gallate formation | Eucalyptus camaldulensis | |
1.1.1.25 | 23.1 | - |
NADPH | pH 6.5, temperature not specified in the publication | Eucalyptus camaldulensis | |
1.1.1.25 | 30.6 | - |
3-dehydroshikimate | pH 10.5, 30°C, EcDQD/SDH2, gallate formation | Eucalyptus camaldulensis |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.1.1.24 | L-quinate + NAD+ | Eucalyptus camaldulensis | - |
3-dehydroquinate + NADH + H+ | - |
r | |
1.1.1.25 | 3-dehydroshikimate + NADP+ | Eucalyptus camaldulensis | - |
gallate + NADPH + H+ | - |
r | |
1.1.1.25 | shikimate + NADP+ | Eucalyptus camaldulensis | - |
3-dehydroshikimate + NADPH + H+ | - |
r |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
1.1.1.24 | Eucalyptus camaldulensis | A0A5H2WXM2 | - |
- |
1.1.1.24 | Eucalyptus camaldulensis | A0A5H2X0F8 | - |
- |
1.1.1.25 | Eucalyptus camaldulensis | A0A5H2WVH6 | - |
- |
1.1.1.25 | Eucalyptus camaldulensis | A0A5H2WZU5 | - |
- |
1.1.1.25 | Eucalyptus camaldulensis | A0A5H2X4C4 | - |
- |
4.2.1.10 | Eucalyptus camaldulensis | A0A5H2WZU5 | cf. EC 1.1.1.25 | - |
4.2.1.10 | Eucalyptus camaldulensis | A0A5H2X4C4 | cf. EC 1.1.1.25 | - |
EC Number | Purification (Comment) | Organism |
---|---|---|
1.1.1.24 | recombinant GST-tagged enzyme from Escherichia coli strain BL21-CodonPlus(DE3)-RIL by glutathione affinity chromatography and tag cleavage through thrombin | Eucalyptus camaldulensis |
1.1.1.25 | - |
Eucalyptus camaldulensis |
1.1.1.25 | recombinant GST-tagged enzyme from Escherichia coli strain BL21-CodonPlus(DE3)-RIL by glutathione affinity chromatography and tag cleavage through thrombin | Eucalyptus camaldulensis |
4.2.1.10 | - |
Eucalyptus camaldulensis |
EC Number | Source Tissue | Comment | Organism | Textmining |
---|---|---|---|---|
1.1.1.24 | leaf | highest expression level | Eucalyptus camaldulensis | - |
1.1.1.24 | additional information | comparison of EcDQD/SDH, UGT84A25a/b, and UGT84A26a/b expression patterns in Eucalyptus camaldulensis, relative EcDQD/SDH mRNA levels in the leaves, stems, and roots are plotted against the relative UGT84A25a/b and UGT84A26a/b mRNA levels in the same samples, and determination of concentrations of the metabolites in the different tissues, overview | Eucalyptus camaldulensis | - |
1.1.1.24 | root | - |
Eucalyptus camaldulensis | - |
1.1.1.24 | stem | lowest expression level | Eucalyptus camaldulensis | - |
1.1.1.25 | leaf | - |
Eucalyptus camaldulensis | - |
1.1.1.25 | leaf | highest expression level | Eucalyptus camaldulensis | - |
1.1.1.25 | additional information | comparison of EcDQD/SDH, UGT84A25a/b, and UGT84A26a/b expression patterns in Eucalyptus camaldulensis, relative EcDQD/SDH mRNA levels in the leaves, stems, and roots are plotted against the relative UGT84A25a/b and UGT84A26a/b mRNA levels in the same samples, and determination of concentrations of the metabolites in the different tissues, overview | Eucalyptus camaldulensis | - |
1.1.1.25 | root | - |
Eucalyptus camaldulensis | - |
1.1.1.25 | root | lowest expression level | Eucalyptus camaldulensis | - |
1.1.1.25 | stem | highest expression level | Eucalyptus camaldulensis | - |
1.1.1.25 | stem | lowest expression level | Eucalyptus camaldulensis | - |
4.2.1.10 | root | - |
Eucalyptus camaldulensis | - |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.1.1.24 | L-quinate + NAD+ | - |
Eucalyptus camaldulensis | 3-dehydroquinate + NADH + H+ | - |
r | |
1.1.1.24 | L-quinate + NADP+ | low activity | Eucalyptus camaldulensis | 3-dehydroquinate + NADPH + H+ | - |
r | |
1.1.1.24 | additional information | product analysis by GC-MS | Eucalyptus camaldulensis | ? | - |
- |
|
1.1.1.25 | 3-dehydroshikimate + NADP+ | - |
Eucalyptus camaldulensis | gallate + NADPH + H+ | - |
r | |
1.1.1.25 | 3-dehydroshikimate + NADPH + H+ | - |
Eucalyptus camaldulensis | shikimate + NADP+ | - |
r | |
1.1.1.25 | additional information | product analysis by GC-MS. No or poor quinate dehydrogenase activity | Eucalyptus camaldulensis | ? | - |
- |
|
1.1.1.25 | shikimate + NADP+ | - |
Eucalyptus camaldulensis | 3-dehydroshikimate + NADPH + H+ | - |
r | |
4.2.1.10 | 3-dehydroquinate | - |
Eucalyptus camaldulensis | 3-dehydroshikimate + H2O | - |
? |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
1.1.1.24 | QDH | - |
Eucalyptus camaldulensis |
1.1.1.24 | quinate dehydrogenase | - |
Eucalyptus camaldulensis |
1.1.1.25 | dehydroquinate dehydratase/shikimate dehydrogenase | - |
Eucalyptus camaldulensis |
1.1.1.25 | EcDQD | - |
Eucalyptus camaldulensis |
1.1.1.25 | EcDQD/SDH | - |
Eucalyptus camaldulensis |
1.1.1.25 | EcDQD/SDH1 | - |
Eucalyptus camaldulensis |
1.1.1.25 | EcDQD/SDH2 | - |
Eucalyptus camaldulensis |
1.1.1.25 | EcDQD/SDH3 | - |
Eucalyptus camaldulensis |
1.1.1.25 | More | cf. EC 4.2.1.10 | Eucalyptus camaldulensis |
4.2.1.10 | EcDQD/SDH1 | - |
Eucalyptus camaldulensis |
4.2.1.10 | EcDQD/SDH2 | - |
Eucalyptus camaldulensis |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
1.1.1.24 | 30 | - |
assay at | Eucalyptus camaldulensis |
1.1.1.25 | 30 | - |
assay at | Eucalyptus camaldulensis |
EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
1.1.1.24 | 127 | - |
L-quinate | pH 9.0, 30°C, EcDQD/SDH4a, quinate oxidation | Eucalyptus camaldulensis | |
1.1.1.24 | 139 | - |
NAD+ | pH 9.0, 30°C, EcDQD/SDH4a, quinate oxidation | Eucalyptus camaldulensis | |
1.1.1.24 | 281 | - |
3-dehydroquinate | pH 7.5, 30°C, EcDQD/SDH4a, quinate formation | Eucalyptus camaldulensis | |
1.1.1.24 | 298 | - |
NADH | pH 7.5, 30°C, EcDQD/SDH4a, quinate formation | Eucalyptus camaldulensis | |
1.1.1.25 | 2.14 | - |
3-dehydroshikimate | pH 10.5, 30°C, EcDQD/SDH2, gallate formation | Eucalyptus camaldulensis | |
1.1.1.25 | 2.87 | - |
3-dehydroshikimate | pH 10.5, 30°C, EcDQD/SDH2, gallate formation | Eucalyptus camaldulensis | |
1.1.1.25 | 3.67 | - |
3-dehydroshikimate | pH 7.0, 30°C, EcDQD/SDH1, DQD activity | Eucalyptus camaldulensis | |
1.1.1.25 | 27.6 | - |
NADPH | pH 8.5, temperature not specified in the publication | Eucalyptus camaldulensis | |
1.1.1.25 | 27.6 | - |
NADPH | pH 8.5, 30°C, EcDQD/SDH1, shikimate formation | Eucalyptus camaldulensis | |
1.1.1.25 | 30.9 | - |
3-dehydroshikimate | pH 7.0, 30°C, EcDQD/SDH1, DQD activity | Eucalyptus camaldulensis | |
1.1.1.25 | 47 | - |
3-dehydroshikimate | pH 8.5, temperature not specified in the publication | Eucalyptus camaldulensis | |
1.1.1.25 | 47 | - |
3-dehydroshikimate | pH 8.5, 30°C, EcDQD/SDH1, shikimate formation | Eucalyptus camaldulensis | |
1.1.1.25 | 57.6 | - |
3-dehydroshikimate | pH 6.5, temperature not specified in the publication | Eucalyptus camaldulensis | |
1.1.1.25 | 57.6 | - |
3-dehydroshikimate | pH 6.5, 30°C, EcDQD/SDH1, shikimate formation | Eucalyptus camaldulensis | |
1.1.1.25 | 60.4 | - |
3-dehydroshikimate | pH 7.0, 30°C, EcDQD/SDH1, DQD activity | Eucalyptus camaldulensis | |
1.1.1.25 | 70.2 | - |
NADP+ | pH 9.0, temperature not specified in the publication | Eucalyptus camaldulensis | |
1.1.1.25 | 70.2 | - |
NADP+ | pH 9.0, 30°C, EcDQD/SDH1, shikimate oxidation | Eucalyptus camaldulensis | |
1.1.1.25 | 74.1 | - |
NADPH | pH 6.5, temperature not specified in the publication | Eucalyptus camaldulensis | |
1.1.1.25 | 74.1 | - |
NADPH | pH 6.5, 30°C, EcDQD/SDH1, shikimate formation | Eucalyptus camaldulensis | |
1.1.1.25 | 80.7 | - |
shikimate | pH 9.0, temperature not specified in the publication | Eucalyptus camaldulensis | |
1.1.1.25 | 80.7 | - |
shikimate | pH 9.0, 30°C, EcDQD/SDH1, shikimate oxidation | Eucalyptus camaldulensis | |
1.1.1.25 | 135 | - |
NADP+ | pH 9.0, temperature not specified in the publication | Eucalyptus camaldulensis | |
1.1.1.25 | 135 | - |
NADP+ | pH 9.0, 30°C, EcDQD/SDH1, shikimate oxidation | Eucalyptus camaldulensis | |
1.1.1.25 | 146 | - |
shikimate | pH 9.0, temperature not specified in the publication | Eucalyptus camaldulensis | |
1.1.1.25 | 146 | - |
shikimate | pH 9.0, 30°C, EcDQD/SDH1, shikimate oxidation | Eucalyptus camaldulensis | |
1.1.1.25 | 307 | - |
3-dehydroshikimate | pH 6.5, temperature not specified in the publication | Eucalyptus camaldulensis | |
1.1.1.25 | 307 | - |
3-dehydroshikimate | pH 6.5, 30°C, EcDQD/SDH1, shikimate formation | Eucalyptus camaldulensis | |
1.1.1.25 | 389 | - |
NADPH | pH 6.5, temperature not specified in the publication | Eucalyptus camaldulensis | |
1.1.1.25 | 389 | - |
NADPH | pH 6.5, 30°C, EcDQD/SDH1, shikimate formation | Eucalyptus camaldulensis | |
1.1.1.25 | 755 | - |
shikimate | pH 9.0, temperature not specified in the publication | Eucalyptus camaldulensis | |
1.1.1.25 | 755 | - |
shikimate | pH 9.0, 30°C, EcDQD/SDH1, shikimate oxidation | Eucalyptus camaldulensis | |
1.1.1.25 | 792 | - |
NADP+ | pH 9.0, temperature not specified in the publication | Eucalyptus camaldulensis | |
1.1.1.25 | 792 | - |
NADP+ | pH 9.0, 30°C, EcDQD/SDH1, shikimate oxidation | Eucalyptus camaldulensis |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
1.1.1.24 | 7.5 | - |
quinate formation activity | Eucalyptus camaldulensis |
1.1.1.24 | 9 | - |
quinate oxidation | Eucalyptus camaldulensis |
1.1.1.25 | 6.5 | - |
shikimate formation activity | Eucalyptus camaldulensis |
1.1.1.25 | 7 | - |
DQD activity | Eucalyptus camaldulensis |
1.1.1.25 | 7.5 | - |
quinate formation activity | Eucalyptus camaldulensis |
1.1.1.25 | 8.5 | - |
shikimate formation activity | Eucalyptus camaldulensis |
1.1.1.25 | 9 | - |
shikimate and quinate oxidation | Eucalyptus camaldulensis |
1.1.1.25 | 10.5 | - |
gallate formation activity | Eucalyptus camaldulensis |
EC Number | Cofactor | Comment | Organism | Structure |
---|---|---|---|---|
1.1.1.24 | NAD+ | - |
Eucalyptus camaldulensis | |
1.1.1.24 | NADH | - |
Eucalyptus camaldulensis | |
1.1.1.25 | NADP+ | - |
Eucalyptus camaldulensis | |
1.1.1.25 | NADP+ | the enzyme shows a clear preference for NADPH/NADP+ over NADH/ NAD+ | Eucalyptus camaldulensis | |
1.1.1.25 | NADPH | - |
Eucalyptus camaldulensis | |
1.1.1.25 | NADPH | the enzyme shows a clear preference for NADPH/NADP+ over NADH/ NAD+ | Eucalyptus camaldulensis |
EC Number | General Information | Comment | Organism |
---|---|---|---|
1.1.1.24 | evolution | plant SDH enzymes are fused to dehydroquinate dehydratases (DQDs, EC 4.2.1.10) to form bifunctional DQD/SDH enzymes. The DQD activity is observed for EcDQD/SDH1, 2, and 3, but not for EcDQD/SDH4a. Among the active enzymes, EcDQD/SDH1 exhibits the highest DQD activity, followed by EcDQD/SDH2 (about 50% of the EcDQD/SDH1 activity) and EcDQD/SDH3 (about 5% of the EcDQD/SDH1 activity). For shikimate formation from 3-DHS as well as shikimate oxidation to 3-DHS, measurable catalytic activities are detected for EcDQD/SDH1-3, but the activities of EcDQD/SDH2 and 3 are less than 20% of those of EcDQD/SDH1. Regarding the cofactor, EcDQD/SDH1-3 have a clear preference for NADPH/NADP+ over NADH/ NAD+. In contrast, EcDQD/SDH4a and b lack shikimate formation activity. For the reverse reaction, the conversion of shikimate to 3-DHS, EcDQD/SDH4a and b display low enzymatic activity with a preference for NAD+ as the cofactor. Both EcDQD/SDH2 and 3 exhibit relatively high gallate formation activity, in contrast to the low activity of EcDQD/SDH1. The preferred cofactor in this reaction is NADP+. The reversible quinate formation from 3-DHQ is catalyzed only by EcDQD/SDH4a/b, with NADH/NAD+ as the preferred cofactor. The reaction specificity of EcDQD/SDH4a confirms the sequence-based prediction that EcDQD/SDH4a is a functional QDH enzyme. This enzyme should be renamed EcQDHa and its closest relative, EcDQD/SDH4b, should be renamed EcQDHb. The EcDQD/SDH4a and EcDQD/SDH4b genes may represent allelic variants encoding enzymes with 99.2% amino acid identity | Eucalyptus camaldulensis |
1.1.1.25 | evolution | plant SDH enzymes are fused to dehydroquinate dehydratases (DQDs, EC 4.2.1.10) to form bifunctional DQD/SDH enzymes. The DQD activity is observed for EcDQD/SDH1, 2, and 3, but not for EcDQD/SDH4a. Among the active enzymes, EcDQD/SDH1 exhibits the highest DQD activity, followed by EcDQD/SDH2 (about 50% of the EcDQD/SDH1 activity) and EcDQD/SDH3 (about 5% of the EcDQD/SDH1 activity). For shikimate formation from 3-DHS as well as shikimate oxidation to 3-DHS, measurable catalytic activities are detected for EcDQD/SDH1-3, but the activities of EcDQD/SDH2 and 3 are less than 20% of those of EcDQD/SDH1. Regarding the cofactor, EcDQD/SDH1-3 have a clear preference for NADPH/NADP+ over NADH/ NAD+. In contrast, EcDQD/SDH4a and b lack shikimate formation activity. For the reverse reaction, the conversion of shikimate to 3-DHS, EcDQD/SDH4a and b display low enzymatic activity with a preference for NAD+ as the cofactor. Both EcDQD/SDH2 and 3 exhibit relatively high gallate formation activity, in contrast to the low activity of EcDQD/SDH1. The preferred cofactor in this reaction is NADP+ | Eucalyptus camaldulensis |
1.1.1.25 | metabolism | shikimate, quinate, and gallate biosynthesis catalyzed by DQD/SDH family enzymes, overview. Plant DQD/SDHs are proposed to link the shikimate pathway to gallate and quinate metabolism | Eucalyptus camaldulensis |
1.1.1.25 | metabolism | shikimate, quinate, and gallate biosynthesis catalyzed by DQD/SDH family enzymes, overview. Plant DQD/SDHs are proposed to link the shikimate pathway to gallate and quinate metabolism. Isozymes EcDQD/SDH2 and 3 from Eucalyptus camaldulensis catalyze the NADP+-dependent oxidation of 3-dehydroshikimate to produce gallate. In Eucalyptus camaldulensis, EcDQD/SDH2 and 3 are co-expressed with UGT84A25a/b and UGT84A26a/b involved in hydrolyzable tannin biosynthesis, catalyze the synthesis of beta-glucogallin | Eucalyptus camaldulensis |
1.1.1.25 | metabolism | shikimate, quinate, and gallate biosynthesis catalyzed by DQD/SDH family enzymes, overview. Plant DQD/SDHs are proposed to link the shikimate pathway to gallate and quinate metabolism. Isozymes EcDQD/SDH2 and 3 from Eucalyptus camaldulensis catalyze the NADP+-dependent oxidation of 3-dehydroshikimate to produce gallate. In Eucalyptus camaldulensis, EcDQD/SDH2 and 3 are co-expressed with UGT84A25a/b and UGT84A26a/b involved in hydrolyzable tannin biosynthesis, they catalyze the synthesis of beta-glucogallin | Eucalyptus camaldulensis |
1.1.1.25 | additional information | EcDQD/SDH1 enzyme structure homology modeling using the structure of Arabidopsis thaliana AtDQD/SDH (Uniprot ID Q9SQT8) as template, docking of 3-dehydroshikimate into the active site of the models as well as the crystal structure of PDB ID 2O7S, molecular dynamics simulation of protein-ligand interaction | Eucalyptus camaldulensis |
1.1.1.25 | additional information | EcDQD/SDH2 enzyme structure homology modeling using the structure of Arabidopsis thaliana AtDQD/SDH (Uniprot ID Q9SQT8) as template, docking of 3-dehydroshikimate into the active site of the models as well as the crystal structure of PDB ID 2O7S, molecular dynamics simulation of protein-ligand interaction | Eucalyptus camaldulensis |
1.1.1.25 | additional information | EcDQD/SDH3 enzyme structure homology modeling using the structure of Arabidopsis thaliana AtDQD/SDH (Uniprot ID Q9SQT8) as template, docking of 3-dehydroshikimate into the active site of the models as well as the crystal structure of PDB ID 2O7S, molecular dynamics simulation of protein-ligand interaction | Eucalyptus camaldulensis |
1.1.1.25 | physiological function | the tree species Eucalyptus camaldulensis shows exceptionally high tolerance against aluminum, a widespread toxic metal in acidic soils. In the roots of Eucalyptus camaldulensis, aluminum is detoxified via the complexation with oenothein B, a hydrolyzable tannin. The biosynthesis of oenothein B involves dehydroquinate dehydratase/shikimate dehydrogenases (EcDQD/SDHs) which catalyzes the formation of gallate, the phenolic constituent of hydrolyzable tannins | Eucalyptus camaldulensis |
1.1.1.25 | physiological function | the tree species Eucalyptus camaldulensis shows exceptionally high tolerance against aluminum, a widespread toxic metal in acidic soils. In the roots of Eucalyptus camaldulensis, aluminum is detoxified via the complexation with oenothein B, a hydrolyzable tannin. The biosynthesis of oenothein B involves dehydroquinate dehydratase/shikimate dehydrogenases (EcDQD/SDHs) which catalyzes the formation of gallate, the phenolic constituent of hydrolyzable tannins. Two enzymes, EcDQD/SDH2 and 3, in Eucalyptus camaldulensis catalyze the NADP+-dependent oxidation of 3-dehydroshikimate to produce gallate. EcDQD/SDH2 and 3 maintain DQD and SDH activities, resulting in a 3-dehydroshikimate supply for gallate formation | Eucalyptus camaldulensis |
EC Number | kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
1.1.1.24 | 200.7 | - |
3-dehydroquinate | pH 7.5, 30°C, EcDQD/SDH4a, quinate formation | Eucalyptus camaldulensis | |
1.1.1.24 | 774.4 | - |
L-quinate | pH 9.0, 30°C, EcDQD/SDH4a, quinate oxidation | Eucalyptus camaldulensis | |
1.1.1.24 | 2588.5 | - |
NAD+ | pH 9.0, 30°C, EcDQD/SDH4a, quinate oxidation | Eucalyptus camaldulensis | |
1.1.1.24 | 2921.6 | - |
NADH | pH 7.5, 30°C, EcDQD/SDH4a, quinate formation | Eucalyptus camaldulensis | |
1.1.1.25 | 0.07 | - |
3-dehydroshikimate | pH 10.5, 30°C, EcDQD/SDH2, gallate formation | Eucalyptus camaldulensis | |
1.1.1.25 | 0.304 | - |
3-dehydroshikimate | pH 10.5, 30°C, EcDQD/SDH2, gallate formation | Eucalyptus camaldulensis | |
1.1.1.25 | 4.16 | - |
3-dehydroshikimate | pH 7.0, 30°C, EcDQD/SDH3, DQD activity | Eucalyptus camaldulensis | |
1.1.1.25 | 5.32 | - |
3-dehydroshikimate | pH 8.5, temperature not specified in the publication | Eucalyptus camaldulensis | |
1.1.1.25 | 5.32 | - |
3-dehydroshikimate | pH 8.5, 30°C, EcDQD/SDH2, shikimate formation | Eucalyptus camaldulensis | |
1.1.1.25 | 22.17 | - |
shikimate | pH 9.0, 30°C, EcDQD/SDH3, shikimate oxidation | Eucalyptus camaldulensis | |
1.1.1.25 | 22.2 | - |
shikimate | pH 9.0, temperature not specified in the publication | Eucalyptus camaldulensis | |
1.1.1.25 | 65.6 | - |
3-dehydroshikimate | pH 6.5, 30°C, EcDQD/SDH3, shikimate formation | Eucalyptus camaldulensis | |
1.1.1.25 | 65.6 | - |
3-dehydroshikimate | pH 6.5, temperature not specified in the publication | Eucalyptus camaldulensis | |
1.1.1.25 | 71.2 | - |
shikimate | pH 9.0, 30°C, EcDQD/SDH2, shikimate oxidation | Eucalyptus camaldulensis | |
1.1.1.25 | 71.3 | - |
shikimate | pH 9.0, temperature not specified in the publication | Eucalyptus camaldulensis | |
1.1.1.25 | 96 | - |
3-dehydroshikimate | pH 7.0, 30°C, EcDQD/SDH2, DQD activity | Eucalyptus camaldulensis | |
1.1.1.25 | 134.5 | - |
3-dehydroshikimate | pH 7.0, 30°C, EcDQD/SDH1, DQD activity | Eucalyptus camaldulensis | |
1.1.1.25 | 805 | - |
3-dehydroshikimate | pH 6.5, temperature not specified in the publication | Eucalyptus camaldulensis | |
1.1.1.25 | 805.8 | - |
3-dehydroshikimate | pH 6.5, 30°C, EcDQD/SDH1, shikimate formation | Eucalyptus camaldulensis | |
1.1.1.25 | 1690 | - |
NADP+ | pH 9.0, temperature not specified in the publication | Eucalyptus camaldulensis | |
1.1.1.25 | 1691.6 | - |
NADP+ | pH 9.0, 30°C, EcDQD/SDH3, shikimate oxidation | Eucalyptus camaldulensis | |
1.1.1.25 | 2070 | - |
NADP+ | pH 9.0, temperature not specified in the publication | Eucalyptus camaldulensis | |
1.1.1.25 | 2070.6 | - |
NADP+ | pH 9.0, 30°C, EcDQD/SDH2, shikimate oxidation | Eucalyptus camaldulensis | |
1.1.1.25 | 3207.8 | - |
NADPH | pH 6.5, 30°C, EcDQD/SDH3, shikimate formation | Eucalyptus camaldulensis | |
1.1.1.25 | 3210 | - |
NADPH | pH 6.5, temperature not specified in the publication | Eucalyptus camaldulensis | |
1.1.1.25 | 6300 | - |
NADPH | pH 8.5, temperature not specified in the publication | Eucalyptus camaldulensis | |
1.1.1.25 | 6301.4 | - |
NADPH | pH 8.5, 30°C, EcDQD/SDH2, shikimate formation | Eucalyptus camaldulensis | |
1.1.1.25 | 7858.6 | - |
NADPH | pH 6.5, 30°C, EcDQD/SDH1, shikimate formation | Eucalyptus camaldulensis | |
1.1.1.25 | 7860 | - |
NADPH | pH 6.5, temperature not specified in the publication | Eucalyptus camaldulensis | |
1.1.1.25 | 14000 | - |
NADP+ | pH 9.0, temperature not specified in the publication | Eucalyptus camaldulensis | |
1.1.1.25 | 14017.7 | - |
NADP+ | pH 9.0, 30°C, EcDQD/SDH1, shikimate oxidation | Eucalyptus camaldulensis | |
1.1.1.25 | 23159.5 | - |
shikimate | pH 9.0, 30°C, EcDQD/SDH1, shikimate oxidation | Eucalyptus camaldulensis | |
1.1.1.25 | 23200 | - |
shikimate | pH 9.0, temperature not specified in the publication | Eucalyptus camaldulensis |