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Literature summary extracted from

  • Marathe, A.; Krishnan, V.; Vinutha, T.; Dahuja, A.; Jolly, M.; Sachdev, A.
    Exploring the role of inositol 1,3,4-trisphosphate 5/6 kinase-2 (GmITPK2) as a dehydration and salinity stress regulator in Glycine max (L.) Merr. through heterologous expression in E. coli (2018), Plant Physiol. Biochem., 123, 331-341 .
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.7.1.159 gene Itpk2, genetic organization and gene structure, isozyme sequence comparisons, in-silico analysis of the promoter region of ITPKs, the promoter region of GmITPK2 shows the presence of two MYB binding elements for drought inducibility and one for abscisic acid response, quantitative RT-PCR enzyme expression analysis. The His5-tagged GmITPK2-expressing recombinant Escherichia coli strain BL21(DE3) cells show tolerance to drought and salinity and thus demonstrate the functional redundancy of the gene across taxa Glycine max

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.7.1.159 cytosol
-
 Glycine max 5829
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.7.1.159 Mg2+ required Glycine max

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.7.1.159 ATP + 1D-myo-inositol 1,3,4-trisphosphate Glycine max
-
 ADP + 1D-myo-inositol 1,3,4,5-tetrakisphosphate
-
 ?
2.7.1.159 ATP + 1D-myo-inositol 1,3,4-trisphosphate Glycine max
-
 ADP + 1D-myo-inositol 1,3,4,6-tetrakisphosphate
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.7.1.159 Glycine max A7X665 cv. Pusa-16, grown in the fields of Division of Genetics, I.A.R.I, New Delhi
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.7.1.159 recombinant His5-tagged GmITPK2 from Escherichia coli strain BL21(DE3) by nickel affinity chromatography Glycine max

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.7.1.159 seed
-
 Glycine max
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.1.159 ATP + 1D-myo-inositol 1,3,4-trisphosphate
-
 Glycine max ADP + 1D-myo-inositol 1,3,4,5-tetrakisphosphate
-
 ?
2.7.1.159 ATP + 1D-myo-inositol 1,3,4-trisphosphate
-
 Glycine max ADP + 1D-myo-inositol 1,3,4,6-tetrakisphosphate
-
 ?

Subunits

EC Number Subunits Comment Organism
2.7.1.159 ? x * 35000, recombinant His5-tagged enzyme, SDS-PAGE Glycine max

Synonyms

EC Number Synonyms Comment Organism
2.7.1.159 GmItpk2
-
 Glycine max
2.7.1.159 inositol 1,3,4-trisphosphate 5/6 kinase-2
-
 Glycine max

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.7.1.159 37
-
 in vivo assay at Glycine max

Cofactor

EC Number Cofactor Comment Organism Structure
2.7.1.159 ATP
-
 Glycine max

Expression

EC Number Organism Comment Expression
2.7.1.159 Glycine max expression profiling of the GmITPK transcripts under different experimental conditions of drought and salinity stress. Plants treated with 200 mM NaCl, the transcript expression of GmITPK2 is maximal 1 h after treatment i.e. 7.09fold and gradually reduced to 2.82fold 8 h after treatment. A steady upregulation is observed in plants treated with 0.1 mM abscisic acid as the transcript accumulation increased gradually with time ranging from 1.0 to 14fold. In PEG-induced drought stress the transcript accumulation is maximal (6.6fold) 1 h after treatment and steadily decreases to 3.4 8 h after treatment. The transcript profiles show a higher fold change in the expression GmITPK2 alone, compared to remaining members of GmITPK gene family additional information

General Information

EC Number General Information Comment Organism
2.7.1.159 physiological function inositol 1,3,4, trisphosphate 5/6 kinase (ITPK), a polyphosphate kinase that converts inositol 1,3,4-trisphosphate to inositol 1,3,4,5/6-tetraphosphate, averting the inositol phosphate pool towards phytic acid (PA) biosynthesis, is a key regulator that exists in four different isoforms in soybean. Role of inositol 1,3,4-trisphosphate 5/6 kinase-2 (GmITPK2) as a dehydration and salinity stress regulator in Glycine max. The significantly higher expression of GmITPK2 under drought and salinity stress suggests its role in providing tolerance mechanism against abiotic stress Glycine max