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Literature summary extracted from

  • Liu, Y.; Guerard, F.; Hodges, M.; Jossier, M.
    Phosphomimetic T335D mutation of hydroxypyruvate reductase 1 modifies cofactor specificity and impacts Arabidopsis growth in air (2020), Plant Physiol., 183, 194-205 .
    View publication on PubMed

Protein Variants

EC Number Protein Variants Comment Organism
1.1.1.26 T335A nonphosphorylatable variant Arabidopsis thaliana
1.1.1.26 T335D phosphomimetic variant, exhibits reduced NADH-dependent hydroxypyruvate reductase activity while showing improved NADPH-dependent activity Arabidopsis thaliana
1.1.1.29 T335A nonphosphorylatable variant Arabidopsis thaliana
1.1.1.29 T335D phosphomimetic variant, exhibits reduced NADH-dependent hydroxypyruvate reductase activity while showing improved NADPH-dependent activity Arabidopsis thaliana

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.1.1.26 0.0058
-
 NADH mutant T335D, pH 6.2, 25°C Arabidopsis thaliana
1.1.1.26 0.01
-
 NADH mutant T335A, pH 6.2, 25°C Arabidopsis thaliana
1.1.1.26 0.01
-
 NADH wild-type, pH 6.2, 25°C Arabidopsis thaliana
1.1.1.26 0.271
-
 NADPH mutant T335D, pH 6.2, 25°C Arabidopsis thaliana
1.1.1.26 0.353
-
 NADPH wild-type, pH 6.2, 25°C Arabidopsis thaliana
1.1.1.26 0.408
-
 NADPH mutant T335A, pH 6.2, 25°C Arabidopsis thaliana
1.1.1.26 9.727
-
 glyoxylate mutant T335D, cosubstrate NADH, pH 6.2, 25°C Arabidopsis thaliana
1.1.1.26 15.195
-
 glyoxylate mutant T335A, cosubstrate NADH, pH 6.2, 25°C Arabidopsis thaliana
1.1.1.26 16.589
-
 glyoxylate mutant T335A, cosubstrate NADPH, pH 6.2, 25°C Arabidopsis thaliana
1.1.1.26 17.366
-
 glyoxylate wild-type, cosubstrate NADH, pH 6.2, 25°C Arabidopsis thaliana
1.1.1.26 17.996
-
 glyoxylate wild-type, cosubstrate NADPH, pH 6.2, 25°C Arabidopsis thaliana
1.1.1.26 18.011
-
 glyoxylate mutant T335D, cosubstrate NADPH, pH 6.2, 25°C Arabidopsis thaliana
1.1.1.29 0.018
-
 NADH mutant T335A, pH 6.2, 25°C Arabidopsis thaliana
1.1.1.29 0.018
-
 NADH wild-type, pH 6.2, 25°C Arabidopsis thaliana
1.1.1.29 0.028
-
 NADH mutant T335D, pH 6.2, 25°C Arabidopsis thaliana
1.1.1.29 0.102
-
 NADPH mutant T335D, pH 6.2, 25°C Arabidopsis thaliana
1.1.1.29 0.135
-
 NADPH mutant T335A, pH 6.2, 25°C Arabidopsis thaliana
1.1.1.29 0.147
-
 NADPH wild-type, pH 6.2, 25°C Arabidopsis thaliana
1.1.1.29 0.167
-
 Hydroxypyruvate mutant T335D, cosubstrate NADH, pH 6.2, 25°C Arabidopsis thaliana
1.1.1.29 0.173
-
 Hydroxypyruvate wild-type, cosubstrate NADH, pH 6.2, 25°C Arabidopsis thaliana
1.1.1.29 0.22
-
 Hydroxypyruvate mutant T335A, cosubstrate NADH, pH 6.2, 25°C Arabidopsis thaliana
1.1.1.29 0.995
-
 Hydroxypyruvate mutant T335D, cosubstrate NADPH, pH 6.2, 25°C Arabidopsis thaliana
1.1.1.29 1.428
-
 Hydroxypyruvate wild-type, cosubstrate NADPH, pH 6.2, 25°C Arabidopsis thaliana
1.1.1.29 1.579
-
 Hydroxypyruvate mutant T335A, cosubstrate NADPH, pH 6.2, 25°C Arabidopsis thaliana

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
1.1.1.26 peroxisome
-
 Arabidopsis thaliana 5777
-
1.1.1.29 peroxisome
-
 Arabidopsis thaliana 5777
-

Organism

EC Number Organism UniProt Comment Textmining
1.1.1.26 Arabidopsis thaliana Q9C9W5 catalyzes reactions of EC 1.1.1.29 and EC 1.1.1.26
-
1.1.1.29 Arabidopsis thaliana Q9C9W5 catalyzes reactions of EC 1.1.1.29 and EC 1.1.1.26
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.1.1.26 leaf
-
 Arabidopsis thaliana
-
1.1.1.29 leaf
-
 Arabidopsis thaliana
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.1.26 glyoxylate + NADH + H+
-
 Arabidopsis thaliana glycolate + NAD+
-
 ?
1.1.1.26 hydroxypyruvate + NADH + H+ reaction of EC 1.1.1.29 Arabidopsis thaliana D-glycerate + NAD+
-
 ?
1.1.1.26 hydroxypyruvate + NADPH + H+ reaction of EC 1.1.1.29, NADPH is a poor cofactor for wild-type Arabidopsis thaliana D-glycerate + NADP+
-
 ?
1.1.1.29 glyoxylate + NADH + H+ reaction of EC 1.1.1.26 Arabidopsis thaliana glycolate + NAD+
-
 ?
1.1.1.29 hydroxypyruvate + NADH + H+
-
 Arabidopsis thaliana D-glycerate + NAD+
-
 ?
1.1.1.29 hydroxypyruvate + NADPH + H+ NADPH is a poor cofactor for wild-type Arabidopsis thaliana D-glycerate + NADP+
-
 ?

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.1.1.26 0.52
-
 glyoxylate mutant T335A, cosubstrate NADPH, pH 6.2, 25°C Arabidopsis thaliana
1.1.1.26 0.56
-
 glyoxylate wild-type, cosubstrate NADPH, pH 6.2, 25°C Arabidopsis thaliana
1.1.1.26 0.56
-
 NADPH wild-type, pH 6.2, 25°C Arabidopsis thaliana
1.1.1.26 0.62
-
 NADPH mutant T335A, pH 6.2, 25°C Arabidopsis thaliana
1.1.1.26 0.85
-
 NADPH mutant T335D, pH 6.2, 25°C Arabidopsis thaliana
1.1.1.26 0.92
-
 glyoxylate mutant T335D, cosubstrate NADPH, pH 6.2, 25°C Arabidopsis thaliana
1.1.1.26 59
-
 glyoxylate mutant T335A, cosubstrate NADH, pH 6.2, 25°C Arabidopsis thaliana
1.1.1.26 60
-
 NADH mutant T335A, pH 6.2, 25°C Arabidopsis thaliana
1.1.1.26 62
-
 NADH wild-type, pH 6.2, 25°C Arabidopsis thaliana
1.1.1.26 68
-
 glyoxylate wild-type, cosubstrate NADH, pH 6.2, 25°C Arabidopsis thaliana
1.1.1.26 75
-
 glyoxylate mutant T335D, cosubstrate NADH, pH 6.2, 25°C Arabidopsis thaliana
1.1.1.26 91
-
 NADH mutant T335D, pH 6.2, 25°C Arabidopsis thaliana
1.1.1.29 139
-
 Hydroxypyruvate mutant T335D, cosubstrate NADH, pH 6.2, 25°C Arabidopsis thaliana
1.1.1.29 144
-
 NADH mutant T335D, pH 6.2, 25°C Arabidopsis thaliana
1.1.1.29 148
-
 NADPH mutant T335A, pH 6.2, 25°C Arabidopsis thaliana
1.1.1.29 150
-
 Hydroxypyruvate mutant T335A, cosubstrate NADPH, pH 6.2, 25°C Arabidopsis thaliana
1.1.1.29 160
-
 Hydroxypyruvate wild-type, cosubstrate NADPH, pH 6.2, 25°C Arabidopsis thaliana
1.1.1.29 162
-
 NADPH wild-type, pH 6.2, 25°C Arabidopsis thaliana
1.1.1.29 206
-
 Hydroxypyruvate mutant T335D, cosubstrate NADPH, pH 6.2, 25°C Arabidopsis thaliana
1.1.1.29 218
-
 NADH mutant T335A, pH 6.2, 25°C Arabidopsis thaliana
1.1.1.29 230
-
 NADPH mutant T335D, pH 6.2, 25°C Arabidopsis thaliana
1.1.1.29 248
-
 NADH wild-type, pH 6.2, 25°C Arabidopsis thaliana
1.1.1.29 253
-
 Hydroxypyruvate mutant T335A, cosubstrate NADH, pH 6.2, 25°C Arabidopsis thaliana
1.1.1.29 284
-
 Hydroxypyruvate wild-type, cosubstrate NADH, pH 6.2, 25°C Arabidopsis thaliana

Cofactor

EC Number Cofactor Comment Organism Structure
1.1.1.26 NADH
-
 Arabidopsis thaliana
1.1.1.26 NADPH NADPH is a poor cofactor for wild-type Arabidopsis thaliana
1.1.1.29 NADH
-
 Arabidopsis thaliana
1.1.1.29 NADPH NADPH is a poor cofactor for wild-type Arabidopsis thaliana

General Information

EC Number General Information Comment Organism
1.1.1.26 metabolism HPR1 phosphomimetic variant T335D exhibits reduced NADH-dependent hydroxypyruvate reductase activity but improved NADPH-dependent activity. Complementation of the Arabidopsis HPR1 mutant by either wild-type HPR1 or HPR1 nonphosphorylatable mutant T335A fully complements the photorespiratory growth phenotype of the HPR1 mutant in ambient air, whereas HPR1 T335D-containing HPR1 mutant plants remain smaller and have lower photosynthetic CO2 assimilation rates. These phenotypes were associated with subtle perturbations in the photorespiratory cycle of HPR1 T335D-complemented HPR1 mutant rosettes compared to all other HPR1-containing lines Arabidopsis thaliana
1.1.1.29 metabolism HPR1 phosphomimetic variant T335D exhibits reduced NADH-dependent hydroxypyruvate reductase activity but improved NADPH-dependent activity. Complementation of the Arabidopsis HPR1 mutant by either wild-type HPR1 or HPR1 nonphosphorylatable mutant T335A fully complements the photorespiratory growth phenotype of the HPR1 mutant in ambient air, whereas HPR1 T335D-containing HPR1 mutant plants remain smaller and have lower photosynthetic CO2 assimilation rates. These phenotypes were associated with subtle perturbations in the photorespiratory cycle of HPR1 T335D-complemented HPR1 mutant rosettes compared to all other HPR1-containing lines Arabidopsis thaliana