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Literature summary extracted from

  • Kuo, H.; Hsu, Y.; Lin, W.; Chen, K.; Munnik, T.; Brearley, C.; Chiou, T.
    Arabidopsis inositol phosphate kinases IPK1 and ITPK1 constitute a metabolic pathway in maintaining phosphate homeostasis (2018), Plant J., 95, 613-630 .
    View publication on PubMed

Protein Variants

EC Number Protein Variants Comment Organism
2.7.1.134 additional information generation of itpk1-1 mutants, phenotype, overview. Genetic interaction analysis of bifunctional ITPK1 (EC 2.7.1.159/EC 2.7.1.134) and IPK1 (EC 2.7.1.158) with a genetic cross between ipk1-1 and itpk1 mutants. The ipk1-1 itpk1 double mutants exhibit more severe growth defects than single mutants and those that proceed to the reproductive stage bore aborted seeds. Common elevation of D/L-Ins(3,4,5,6)P4 in itpk1 and ipk1-1 mutants Arabidopsis thaliana
2.7.1.158 D368A site-directed mutagenesis, mutant IPK1D368A shows loss of kinase activity in vitro Arabidopsis thaliana
2.7.1.158 K168A site-directed mutagenesis, mutant IPK1K168AD shows loss of kinase activity in vitro Arabidopsis thaliana
2.7.1.158 additional information generation of ipk1-1 mutants, phenotype, overview. Genetic interaction analysis of ITPK1 (EC 2.7.1.134) and IPK1 (EC 2.7.1.158) with a genetic cross between ipk1-1 and itpk1 mutants. The ipk1-1 itpk1 double mutants exhibit more severe growth defects than single mutants and those that, proceeded to the reproductive stage, bore aborted seeds. Common elevation of D/L-Ins(3,4,5,6)P4 in itpk1 and ipk1-1 mutants Arabidopsis thaliana
2.7.1.159 additional information generation of itpk1-1 mutants, phenotype, overview. Genetic interaction analysis of bifunctional ITPK1 (EC 2.7.1.159/EC 2.7.1.134) and IPK1 (EC 2.7.1.158) with a genetic cross between ipk1-1 and itpk1 mutants. The ipk1-1 itpk1 double mutants exhibit more severe growth defects than single mutants and those that proceeded to the reproductive stage bore aborted seeds. Common elevation of D/L-Ins(3,4,5,6)P4 in itpk1 and ipk1-1 mutants Arabidopsis thaliana

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.7.1.134 Mg2+ required Arabidopsis thaliana
2.7.1.158 Mg2+ required Arabidopsis thaliana
2.7.1.159 Mg2+ required Arabidopsis thaliana

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.7.1.134 ATP + 1D-myo-inositol 3,4,5,6-tetrakisphosphate Arabidopsis thaliana
-
 ADP + 1D-myo-inositol 1,3,4,5,6-pentakisphosphate
-
 ?
2.7.1.158 ATP + 1D-myo-inositol 1,3,4,5,6-pentakisphosphate Arabidopsis thaliana
-
 ADP + 1D-myo-inositol hexakisphosphate
-
 ?
2.7.1.159 ATP + 1D-myo-inositol 1,3,4-trisphosphate Arabidopsis thaliana
-
 ADP + 1D-myo-inositol 1,3,4,5-tetrakisphosphate
-
 ?
2.7.1.159 ATP + 1D-myo-inositol 1,3,4-trisphosphate Arabidopsis thaliana
-
 ADP + 1D-myo-inositol 1,3,4,6-tetrakisphosphate
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.7.1.134 Arabidopsis thaliana Q9SBA5
-
-
2.7.1.158 Arabidopsis thaliana Q93YN9
-
-
2.7.1.159 Arabidopsis thaliana Q9SBA5
-
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.7.1.134 seed
-
 Arabidopsis thaliana
-
2.7.1.159 seed
-
 Arabidopsis thaliana
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.1.134 ATP + 1D-myo-inositol 3,4,5,6-tetrakisphosphate
-
 Arabidopsis thaliana ADP + 1D-myo-inositol 1,3,4,5,6-pentakisphosphate
-
 ?
2.7.1.158 ATP + 1D-myo-inositol 1,3,4,5,6-pentakisphosphate
-
 Arabidopsis thaliana ADP + 1D-myo-inositol hexakisphosphate
-
 ?
2.7.1.159 ATP + 1D-myo-inositol 1,3,4-trisphosphate
-
 Arabidopsis thaliana ADP + 1D-myo-inositol 1,3,4,5-tetrakisphosphate
-
 ?
2.7.1.159 ATP + 1D-myo-inositol 1,3,4-trisphosphate
-
 Arabidopsis thaliana ADP + 1D-myo-inositol 1,3,4,6-tetrakisphosphate
-
 ?

Synonyms

EC Number Synonyms Comment Organism
2.7.1.134 inositol 1,3,4-trisphosphate 5/6-kinase 1
-
 Arabidopsis thaliana
2.7.1.134 ITPK1
-
 Arabidopsis thaliana
2.7.1.134 additional information see also EC 2.7.1.159 Arabidopsis thaliana
2.7.1.158 inositol pentakisphosphate 2-kinase
-
 Arabidopsis thaliana
2.7.1.158 Ipk1
-
 Arabidopsis thaliana
2.7.1.159 inositol 1,3,4-trisphosphate 5/6-kinase 1
-
 Arabidopsis thaliana
2.7.1.159 ITPK1
-
 Arabidopsis thaliana
2.7.1.159 additional information see also EC 2.7.1.134 Arabidopsis thaliana

Cofactor

EC Number Cofactor Comment Organism Structure
2.7.1.134 ATP
-
 Arabidopsis thaliana
2.7.1.158 ATP
-
 Arabidopsis thaliana
2.7.1.159 ATP
-
 Arabidopsis thaliana

General Information

EC Number General Information Comment Organism
2.7.1.134 malfunction although both ipk1-1 and itpk1 mutants exhibit decreased levels of InsP6 (phytate) and diphosphoinositol pentakisphosphate (PP-InsP5, InsP7), disruption of another ITPK family enzyme, ITPK4, which correspondingly causes depletion of InsP6 and InsP7, does not display similar phosphate-related phenotypes, which precludes these InsP species from being effectors. Notably, the level of D/L-Ins(3,4,5,6)P4 is concurrently elevated in both ipk1-1 and itpk1 mutants, which demonstrates a specific correlation with the misregulated phosphate phenotypes. The level of D/L-Ins(3,4,5,6)P4 is not responsive to phosphate starvation that instead manifests a shoot-specific increase in the InsP7 level. ITPK1 overexpression significantly decreases phosphate uptake activity, in contrast to the elevated uptake activity shown by itpk1 mutants. In addition, several PSR genes are downregulated in ITPK1-overexpressing lines compared with the wild-type (e.g. PHT1:2, SPX1, AT4, IPS1 and PAP17) Arabidopsis thaliana
2.7.1.134 metabolism the kinase activity of inositol pentakisphosphate 2-kinase (IPK1) is required for phytate (inositol hexakisphosphate, InsP6) synthesis, and is indispensable for maintaining phosphate homeostasis under phosphate-replete conditions. Inositol 1,3,4-trisphosphate 5/6-kinase 1 (ITPK1) plays an equivalent role. Genetic dissection of the roles for InsP and PP-InsP biosynthesis enzymes in regulation of phosphate homeostasis, overview Arabidopsis thaliana
2.7.1.134 physiological function the enzyme is required for phytate (inositol hexakisphosphate, InsP6) synthesis and involved in maintaining phosphate homeostasis under phosphate-replete conditions Arabidopsis thaliana
2.7.1.158 malfunction in contrast to wild-type IPK1, which is able to restore the phosphate content of the ipk1-1 mutant to wild-type level, both kinase-inactive IPK1 forms fail to complement excessive phosphate accumulation and PSR gene activation in ipk1-1. Although both ipk1-1 and itpk1 mutants exhibit decreased levels of InsP6 (phytate) and diphosphoinositol pentakisphosphate (PP-InsP5; InsP7), disruption of another ITPK family enzyme, ITPK4, which correspondingly causes depletion of InsP6 and InsP7, does not display similar phosphate-related phenotypes, which precludes these InsP species from being effectors. Notably, the level of D/L-Ins(3,4,5,6)P4 is concurrently elevated in both ipk1-1 and itpk1 mutants, which demonstrates a specific correlation with the misregulated phosphate phenotypes. The level of D/L-Ins(3,4,5,6)P4 is not responsive to phosphate starvation that instead manifests a shoot-specific increase in the InsP7 level. Misregulation of phosphate homeostasis in ipk1-1 is not caused by defective InsP6-mediated mRNA export. Neither of the kinase-inactive IPK1 mutants K168A and D368A complement the PSR-like RSA phenotypes (i.e. reduced primary root and enhanced lateral root growth) of ipk1-1 mutant. In addition to the decreased InsP6 level, levels of InsP7 and InsP8 are also reduced in ipk1-1 mutants Arabidopsis thaliana
2.7.1.158 metabolism genetic dissection of the roles for InsP and PP-InsP biosynthesis enzymes in regulation of phosphate homeostasis, overview Arabidopsis thaliana
2.7.1.158 physiological function the kinase activity of inositol pentakisphosphate 2-kinase (IPK1) is required for phytate (inositol hexakisphosphate, InsP6) synthesis, and is indispensable for maintaining phosphate homeostasis under phosphate-replete conditions. Inositol 1,3,4-trisphosphate 5/6-kinase 1 (ITPK1) plays an equivalent role. In addition to regulating the phosphate content, the kinase activity of IPK1 is also required for root system architecture Arabidopsis thaliana
2.7.1.159 malfunction although both ipk1-1 and itpk1 mutants exhibit decreased levels of InsP6 (phytate) and diphosphoinositol pentakisphosphate (PP-InsP5, InsP7), disruption of another ITPK family enzyme, ITPK4, which correspondingly causes depletion of InsP6 and InsP7, does not display similar phosphate-related phenotypes, which precludes these InsP species from being effectors. Notably, the level of D/L-Ins(3,4,5,6)P4 is concurrently elevated in both ipk1-1 and itpk1 mutants, which demonstrates a specific correlation with the misregulated phosphate phenotypes. The level of D/L-Ins(3,4,5,6)P4 is not responsive to phosphate starvation that instead manifests a shoot-specific increase in the InsP7 level. ITPK1 overexpression significantly decreases phosphate uptake activity, in contrast to the elevated uptake activity shown by itpk1 mutants. In addition, several PSR genes are downregulated in ITPK1-overexpressing lines compared with the wild-type (e.g. PHT1:2, SPX1, AT4, IPS1 and PAP17) Arabidopsis thaliana
2.7.1.159 metabolism the kinase activity of inositol pentakisphosphate 2-kinase (IPK1) is required for phytate (inositol hexakisphosphate, InsP6) synthesis, and is indispensable for maintaining phosphate homeostasis under phosphate-replete conditions. Inositol 1,3,4-trisphosphate 5/6-kinase 1 (ITPK1) plays an equivalent role. Genetic dissection of the roles for InsP and PP-InsP biosynthesis enzymes in regulation of phosphate homeostasis, overview Arabidopsis thaliana
2.7.1.159 physiological function the enzyme is required for phytate (inositol hexakisphosphate, InsP6) synthesis and involved in maintaining phosphate homeostasis under phosphate-replete conditions Arabidopsis thaliana