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Literature summary extracted from

  • Hartman, M.; Figueroa, C.; Arias, D.; Iglesias, A.
    Inhibition of recombinant aldose-6-phosphate reductase from peach leaves by hexose-phosphates, inorganic phosphate and oxidants (2017), Plant Cell Physiol., 58, 145-155 .
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.1.1.200 expressed in Escherichia coli BL21 Star (DE3) cells Prunus persica

General Stability

EC Number General Stability Organism
1.1.1.200 enzyme thiolation with oxidized glutathione protects the enzyme from insolubilization caused by diamide, while incubation with NADP+ completely prevents enzyme precipitation Prunus persica

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.1.1.200 D-fructose 1,6-bisphosphate about 40% inhibition at 20 mM Prunus persica
1.1.1.200 D-fructose 6-phosphate about 40% inhibition at 20 mM Prunus persica
1.1.1.200 D-galactose 1-phosphate about 40% inhibition at 20 mM Prunus persica
1.1.1.200 D-glucose 1-phosphate about 55% inhibition at 20 mM Prunus persica
1.1.1.200 D-ribose 5-phosphate about 40% inhibition at 20 mM Prunus persica
1.1.1.200 Diamide enzymatic activity decreases to approximately 7% at 500 mM diamide Prunus persica
1.1.1.200 H2O2 H2O2 oxidation causes enzyme inactivation in a time- and concentration-dependent manner Prunus persica
1.1.1.200 mannose 1-phosphate about 40% inhibition at 20 mM Prunus persica
1.1.1.200 mannose 6-phosphate about 30% inhibition at 20 mM Prunus persica
1.1.1.200 additional information no inhibition by D-trehalose 6-phosphate, D-fructose 2,6-bisphosphate, D-xylitol, D-glucose and D-fructose. Not affected by 5 mM GSSG Prunus persica
1.1.1.200 phosphate competitive inhibitor Prunus persica

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.1.1.200 0.031
-
 NADPH at pH 8.0 and 25°C Prunus persica
1.1.1.200 0.059
-
 NADP+ at pH 8.0 and 25°C Prunus persica
1.1.1.200 3.5
-
 D-glucose 6-phosphate at pH 8.0 and 25°C Prunus persica
1.1.1.200 11
-
 D-glucose 6-phosphate at pH 8.0 and 25°C Prunus persica

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.1.1.200 additional information no significant effects are observed when the activity of the enzyme is assayed in the presence of 5 mM Mg2+ or Ca2+ Prunus persica

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.1.1.200 67000
-
 gel filtration Prunus persica

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.1.1.200 D-glucose 6-phosphate + NADPH + H+ Prunus persica
-
 D-sorbitol 6-phosphate + NADP+
-
 r
1.1.1.200 D-sorbitol 6-phosphate + NADP+ Prunus persica
-
 D-glucose 6-phosphate + NADPH + H+
-
 r

Organism

EC Number Organism UniProt Comment Textmining
1.1.1.200 Prunus persica A5JUQ9 cultivar Flordakin
-

Oxidation Stability

EC Number Oxidation Stability Organism
1.1.1.200 NADP+ protects the enzyme from oxidative insolubilization Prunus persica

Purification (Commentary)

EC Number Purification (Comment) Organism
1.1.1.200 HisTrap column chromatography Prunus persica

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.1.1.200 leaf
-
 Prunus persica
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.1.200 D-glucose 6-phosphate + NADPH + H+
-
 Prunus persica D-sorbitol 6-phosphate + NADP+
-
 r
1.1.1.200 D-sorbitol 6-phosphate + NADP+
-
 Prunus persica D-glucose 6-phosphate + NADPH + H+
-
 r
1.1.1.200 additional information almost no activity with mannose 6-phosphate or ribose 5-phosphate, no activity with NADH and NAD+ Prunus persica ?
-
-

Subunits

EC Number Subunits Comment Organism
1.1.1.200 homodimer 2 * 35000, SDS-PAGE Prunus persica
1.1.1.200 homodimer 2 * 34800, calculated from amino acid sequence Prunus persica

Synonyms

EC Number Synonyms Comment Organism
1.1.1.200 Ald6PRase
-
 Prunus persica

Cofactor

EC Number Cofactor Comment Organism Structure
1.1.1.200 NADP+
-
 Prunus persica
1.1.1.200 NADPH
-
 Prunus persica

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
1.1.1.200 9.07
-
 D-glucose 1-phosphate at pH 8.0 and 25°C Prunus persica
1.1.1.200 26
-
 D-fructose 1,6-bisphosphate at pH 8.0 and 25°C Prunus persica
1.1.1.200 29.6
-
 phosphate at pH 8.0 and 25°C Prunus persica
1.1.1.200 55.4
-
 D-fructose 6-phosphate at pH 8.0 and 25°C Prunus persica

pI Value

EC Number Organism Comment pI Value Maximum pI Value
1.1.1.200 Prunus persica calculated from amino acid sequence
-
 7.9