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Literature summary extracted from

  • Knaus, T.; Cariati, L.; Masman, M.F.; Mutti, F.G.
    In vitro biocatalytic pathway design orthogonal network for the quantitative and stereospecific amination of alcohols (2017), Org. Biomol. Chem., 15, 8313-8325 .
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.1.1.B3 expressed in Escherichia coli C43 cells Thermoanaerobacter ethanolicus

Protein Variants

EC Number Protein Variants Comment Organism
1.1.1.B3 I86A the mutant shows reversed stereoselectivity (R) compared to the wild type enzyme Thermoanaerobacter ethanolicus
1.1.1.B3 W110A the mutant shows imperfect wild type stereoselectivity (S) Thermoanaerobacter ethanolicus
1.1.1.B3 W110A/I86A the mutant enzyme is completely non-stereoselective for the reduction of 2-hexanone.Additionally, an extremely low level of stereoselectivity (i.e. from 10% to 24%) is observed for the reduction of aryl-aliphatic ketones and aliphatic ketones Thermoanaerobacter ethanolicus

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.1.1.B3 Zn2+ contains zinc Thermoanaerobacter ethanolicus

Organism

EC Number Organism UniProt Comment Textmining
1.1.1.B3 Thermoanaerobacter ethanolicus
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.1.1.B3 Strep-Tactin column chromatography Thermoanaerobacter ethanolicus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.1.B3 (S)-1-phenyl 2-propanol + NADP+
-
 Thermoanaerobacter ethanolicus phenylacetone + NADPH + H+
-
 ?
1.1.1.B3 1-(3-fluorophenyl)ethan-1-ol + NADP+
-
 Thermoanaerobacter ethanolicus 1-(3-fluorophenyl)ethan-1-one + NADPH + H+
-
 ?
1.1.1.B3 1-(3-methoxyphenyl)propan-2-ol + NADP+
-
 Thermoanaerobacter ethanolicus 1-(3-methoxyphenyl)propan-2-one + NADPH + H+
-
 ?
1.1.1.B3 1-(3-methylphenyl)ethan-1-ol + NADP+
-
 Thermoanaerobacter ethanolicus 1-(3-methylphenyl)ethan-1-one + NADPH + H+
-
 ?
1.1.1.B3 1-(4-fluorophenyl)ethan-1-ol + NADP+
-
 Thermoanaerobacter ethanolicus 1-(4-fluorophenyl)ethan-1-one + NADPH + H+
-
 ?
1.1.1.B3 1-(4-fluorophenyl)propan-2-ol + NADP+
-
 Thermoanaerobacter ethanolicus 1-(4-fluorophenyl)propan-2-one + NADPH + H+
-
 ?
1.1.1.B3 1-(4-methylphenyl)ethan-1-ol + NADP+
-
 Thermoanaerobacter ethanolicus 1-(4-methylphenyl)ethan-1-one + NADPH + H+
-
 ?
1.1.1.B3 1-(4-methylphenyl)propan-2-ol + NADP+
-
 Thermoanaerobacter ethanolicus 1-(4-methylphenyl)propan-2-one + NADPH + H+
-
 ?
1.1.1.B3 1-phenoxypropan-2-ol + NADP+
-
 Thermoanaerobacter ethanolicus 1-phenoxypropan-2-one + NADPH + H+
-
 ?
1.1.1.B3 1-phenylbutan-2-ol + NADP+
-
 Thermoanaerobacter ethanolicus 1-phenylbutan-2-one + NADPH + H+
-
 ?
1.1.1.B3 1-phenylpropan-2-ol + NADP+
-
 Thermoanaerobacter ethanolicus 1-phenylpropan-2-one + NADPH + H+
-
 ?
1.1.1.B3 4-methylpentan-2-ol + NADP+
-
 Thermoanaerobacter ethanolicus 4-methylpentan-2-one + NADPH + H+
-
 ?
1.1.1.B3 4-phenylbutan-2-ol + NADP+
-
 Thermoanaerobacter ethanolicus 4-phenylbutan-2-one + NADPH + H+
-
 ?
1.1.1.B3 heptan-1-ol + NADP+
-
 Thermoanaerobacter ethanolicus heptanal + NADPH + H+
-
 ?
1.1.1.B3 heptan-2-ol + NADP+
-
 Thermoanaerobacter ethanolicus heptan-2-one + NADPH + H+
-
 ?
1.1.1.B3 hexan-1-ol + NADP+
-
 Thermoanaerobacter ethanolicus hexanal + NADPH + H+
-
 ?
1.1.1.B3 hexan-2-ol + NADP+
-
 Thermoanaerobacter ethanolicus hexan-2-one + NADPH + H+
-
 ?
1.1.1.B3 octan-1-ol + NADP+
-
 Thermoanaerobacter ethanolicus octanal + NADPH + H+
-
 ?
1.1.1.B3 octan-2-ol + NADP+
-
 Thermoanaerobacter ethanolicus octan-2-one + NADPH + H+
-
 ?
1.1.1.B3 pentan-1-ol + NADP+
-
 Thermoanaerobacter ethanolicus pentanal + NADPH + H+
-
 ?
1.1.1.B3 pentan-2-ol + NADP+
-
 Thermoanaerobacter ethanolicus pentan-2-one + NADPH + H+
-
 ?

Synonyms

EC Number Synonyms Comment Organism
1.1.1.B3 S-ADH
-
 Thermoanaerobacter ethanolicus

Cofactor

EC Number Cofactor Comment Organism Structure
1.1.1.B3 NADP+
-
 Thermoanaerobacter ethanolicus