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Literature summary extracted from
- NAD+-dependent synthesis of a 5-phospho-ADP-ribosylated RNA/DNA cap by RNA 2-phosphotransferase Tpt1 (2018), Nucleic Acids Res., 46, 9617-9624 .
Cloned(Commentary)
| EC Number | Cloned (Comment) | Organism |
|---|---|---|
| 2.7.1.160 | gene CTHT_0025470, recombinant expression of His6-tagged enzyme in Escherichia coli strain BL21(DE3) | Thermochaetoides thermophila |
| 2.7.1.160 | gene kptA, recombinant expression of His6-tagged enzyme in Escherichia coli strain BL21(DE3) | Acetivibrio thermocellus |
| 2.7.1.160 | gene kptA, recombinant expression of His6-tagged enzyme in Escherichia coli strain BL21(DE3) | Aeropyrum pernix |
| 2.7.1.160 | gene kptA, recombinant expression of His6-tagged enzyme in Escherichia coli strain BL21(DE3) | Pyrococcus horikoshii |
| 2.7.1.160 | gene kptA, recombinant expression of His6-tagged enzyme in Escherichia coli strain BL21(DE3) | Archaeoglobus fulgidus |
| 2.7.1.160 | gene TPT1, recombinant expression of His6-tagged enzyme in Escherichia coli strain BL21(DE3) | Homo sapiens |
| 2.7.1.160 | recombinant expression of His6-tagged wild-type and mutant enzymes in Escherichia coli strain BL21(DE3) | Runella slithyformis |
Protein Variants
| EC Number | Protein Variants | Comment | Organism |
|---|---|---|---|
| 2.7.1.160 | R64A | inactive step 2-defective RslTpt1-R64A mutant | Runella slithyformis |
Inhibitors
| EC Number | Inhibitors | Comment | Organism | Structure |
|---|---|---|---|---|
| 2.7.1.160 | EDTA | - |
Acetivibrio thermocellus |  |
| 2.7.1.160 | EDTA | - |
Aeropyrum pernix | |
| 2.7.1.160 | EDTA | - |
Archaeoglobus fulgidus | |
| 2.7.1.160 | EDTA | - |
Homo sapiens | |
| 2.7.1.160 | EDTA | - |
Pyrococcus horikoshii | |
| 2.7.1.160 | EDTA | - |
Runella slithyformis | |
| 2.7.1.160 | EDTA | - |
Saccharomyces cerevisiae | |
| 2.7.1.160 | EDTA | - |
Thermochaetoides thermophila | |
| 2.7.1.160 | Formamide | - |
Acetivibrio thermocellus | |
| 2.7.1.160 | Formamide | - |
Aeropyrum pernix | |
| 2.7.1.160 | Formamide | - |
Archaeoglobus fulgidus | |
| 2.7.1.160 | Formamide | - |
Homo sapiens | |
| 2.7.1.160 | Formamide | - |
Pyrococcus horikoshii | |
| 2.7.1.160 | Formamide | - |
Runella slithyformis | |
| 2.7.1.160 | Formamide | - |
Saccharomyces cerevisiae | |
| 2.7.1.160 | Formamide | - |
Thermochaetoides thermophila | |
KM Value [mM]
| EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 2.7.1.160 | additional information | - |
additional information | kinetic profile | Aeropyrum pernix |
Natural Substrates/ Products (Substrates)
| EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 2.7.1.160 | 2'-phospho-[ligated tRNA] + NAD+ | Homo sapiens | - |
mature tRNA + ADP-ribose 1'',2''-phosphate + nicotinamide | - |
? | |
| 2.7.1.160 | 2'-phospho-[ligated tRNA] + NAD+ | Saccharomyces cerevisiae | - |
mature tRNA + ADP-ribose 1'',2''-phosphate + nicotinamide | - |
? | |
| 2.7.1.160 | 2'-phospho-[ligated tRNA] + NAD+ | Runella slithyformis | - |
mature tRNA + ADP-ribose 1'',2''-phosphate + nicotinamide | - |
? | |
| 2.7.1.160 | 2'-phospho-[ligated tRNA] + NAD+ | Acetivibrio thermocellus | - |
mature tRNA + ADP-ribose 1'',2''-phosphate + nicotinamide | - |
? | |
| 2.7.1.160 | 2'-phospho-[ligated tRNA] + NAD+ | Thermochaetoides thermophila | - |
mature tRNA + ADP-ribose 1'',2''-phosphate + nicotinamide | - |
? | |
| 2.7.1.160 | 2'-phospho-[ligated tRNA] + NAD+ | Aeropyrum pernix | - |
mature tRNA + ADP-ribose 1'',2''-phosphate + nicotinamide | - |
? | |
| 2.7.1.160 | 2'-phospho-[ligated tRNA] + NAD+ | Pyrococcus horikoshii | - |
mature tRNA + ADP-ribose 1'',2''-phosphate + nicotinamide | - |
? | |
| 2.7.1.160 | 2'-phospho-[ligated tRNA] + NAD+ | Archaeoglobus fulgidus | - |
mature tRNA + ADP-ribose 1'',2''-phosphate + nicotinamide | - |
? | |
| 2.7.1.160 | 2'-phospho-[ligated tRNA] + NAD+ | Aeropyrum pernix ATCC 700893 | - |
mature tRNA + ADP-ribose 1'',2''-phosphate + nicotinamide | - |
? | |
| 2.7.1.160 | 2'-phospho-[ligated tRNA] + NAD+ | Aeropyrum pernix DSM 11879 | - |
mature tRNA + ADP-ribose 1'',2''-phosphate + nicotinamide | - |
? | |
| 2.7.1.160 | 2'-phospho-[ligated tRNA] + NAD+ | Thermochaetoides thermophila IMI 039719 | - |
mature tRNA + ADP-ribose 1'',2''-phosphate + nicotinamide | - |
? | |
| 2.7.1.160 | 2'-phospho-[ligated tRNA] + NAD+ | Acetivibrio thermocellus DSM 1237 | - |
mature tRNA + ADP-ribose 1'',2''-phosphate + nicotinamide | - |
? | |
| 2.7.1.160 | 2'-phospho-[ligated tRNA] + NAD+ | Archaeoglobus fulgidus NBRC 100126 | - |
mature tRNA + ADP-ribose 1'',2''-phosphate + nicotinamide | - |
? | |
| 2.7.1.160 | 2'-phospho-[ligated tRNA] + NAD+ | Archaeoglobus fulgidus ATCC 49558 | - |
mature tRNA + ADP-ribose 1'',2''-phosphate + nicotinamide | - |
? | |
| 2.7.1.160 | 2'-phospho-[ligated tRNA] + NAD+ | Thermochaetoides thermophila DSM 1495 | - |
mature tRNA + ADP-ribose 1'',2''-phosphate + nicotinamide | - |
? | |
| 2.7.1.160 | 2'-phospho-[ligated tRNA] + NAD+ | Pyrococcus horikoshii DSM 12428 | - |
mature tRNA + ADP-ribose 1'',2''-phosphate + nicotinamide | - |
? | |
| 2.7.1.160 | 2'-phospho-[ligated tRNA] + NAD+ | Acetivibrio thermocellus JCM 9322 | - |
mature tRNA + ADP-ribose 1'',2''-phosphate + nicotinamide | - |
? | |
| 2.7.1.160 | 2'-phospho-[ligated tRNA] + NAD+ | Acetivibrio thermocellus NBRC 103400 | - |
mature tRNA + ADP-ribose 1'',2''-phosphate + nicotinamide | - |
? | |
| 2.7.1.160 | 2'-phospho-[ligated tRNA] + NAD+ | Aeropyrum pernix JCM 9820 | - |
mature tRNA + ADP-ribose 1'',2''-phosphate + nicotinamide | - |
? | |
| 2.7.1.160 | 2'-phospho-[ligated tRNA] + NAD+ | Pyrococcus horikoshii NBRC 100139 | - |
mature tRNA + ADP-ribose 1'',2''-phosphate + nicotinamide | - |
? | |
| 2.7.1.160 | 2'-phospho-[ligated tRNA] + NAD+ | Pyrococcus horikoshii JCM 9974 | - |
mature tRNA + ADP-ribose 1'',2''-phosphate + nicotinamide | - |
? | |
| 2.7.1.160 | 2'-phospho-[ligated tRNA] + NAD+ | Acetivibrio thermocellus ATCC 27405 | - |
mature tRNA + ADP-ribose 1'',2''-phosphate + nicotinamide | - |
? | |
| 2.7.1.160 | 2'-phospho-[ligated tRNA] + NAD+ | Acetivibrio thermocellus VPI 7372 | - |
mature tRNA + ADP-ribose 1'',2''-phosphate + nicotinamide | - |
? | |
| 2.7.1.160 | 2'-phospho-[ligated tRNA] + NAD+ | Archaeoglobus fulgidus VC-16 | - |
mature tRNA + ADP-ribose 1'',2''-phosphate + nicotinamide | - |
? | |
| 2.7.1.160 | 2'-phospho-[ligated tRNA] + NAD+ | Thermochaetoides thermophila CBS 144.50 | - |
mature tRNA + ADP-ribose 1'',2''-phosphate + nicotinamide | - |
? | |
| 2.7.1.160 | 2'-phospho-[ligated tRNA] + NAD+ | Aeropyrum pernix NBRC 100138 | - |
mature tRNA + ADP-ribose 1'',2''-phosphate + nicotinamide | - |
? | |
| 2.7.1.160 | 2'-phospho-[ligated tRNA] + NAD+ | Pyrococcus horikoshii ATCC 700860 | - |
mature tRNA + ADP-ribose 1'',2''-phosphate + nicotinamide | - |
? | |
| 2.7.1.160 | 2'-phospho-[ligated tRNA] + NAD+ | Acetivibrio thermocellus NCIMB 10682 | - |
mature tRNA + ADP-ribose 1'',2''-phosphate + nicotinamide | - |
? | |
| 2.7.1.160 | 2'-phospho-[ligated tRNA] + NAD+ | Pyrococcus horikoshii OT-3 | - |
mature tRNA + ADP-ribose 1'',2''-phosphate + nicotinamide | - |
? | |
| 2.7.1.160 | 2'-phospho-[ligated tRNA] + NAD+ | Acetivibrio thermocellus NRRL B-4536 | - |
mature tRNA + ADP-ribose 1'',2''-phosphate + nicotinamide | - |
? | |
| 2.7.1.160 | 2'-phospho-[ligated tRNA] + NAD+ | Archaeoglobus fulgidus JCM 9628 | - |
mature tRNA + ADP-ribose 1'',2''-phosphate + nicotinamide | - |
? | |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 2.7.1.160 | Acetivibrio thermocellus | A3DJX6 | Clostridium thermocellum | - |
| 2.7.1.160 | Acetivibrio thermocellus ATCC 27405 | A3DJX6 | Clostridium thermocellum | - |
| 2.7.1.160 | Acetivibrio thermocellus DSM 1237 | A3DJX6 | Clostridium thermocellum | - |
| 2.7.1.160 | Acetivibrio thermocellus JCM 9322 | A3DJX6 | Clostridium thermocellum | - |
| 2.7.1.160 | Acetivibrio thermocellus NBRC 103400 | A3DJX6 | Clostridium thermocellum | - |
| 2.7.1.160 | Acetivibrio thermocellus NCIMB 10682 | A3DJX6 | Clostridium thermocellum | - |
| 2.7.1.160 | Acetivibrio thermocellus NRRL B-4536 | A3DJX6 | Clostridium thermocellum | - |
| 2.7.1.160 | Acetivibrio thermocellus VPI 7372 | A3DJX6 | Clostridium thermocellum | - |
| 2.7.1.160 | Aeropyrum pernix | Q9YFP5 | - |
- |
| 2.7.1.160 | Aeropyrum pernix ATCC 700893 | Q9YFP5 | - |
- |
| 2.7.1.160 | Aeropyrum pernix DSM 11879 | Q9YFP5 | - |
- |
| 2.7.1.160 | Aeropyrum pernix JCM 9820 | Q9YFP5 | - |
- |
| 2.7.1.160 | Aeropyrum pernix NBRC 100138 | Q9YFP5 | - |
- |
| 2.7.1.160 | Archaeoglobus fulgidus | O29841 | - |
- |
| 2.7.1.160 | Archaeoglobus fulgidus ATCC 49558 | O29841 | - |
- |
| 2.7.1.160 | Archaeoglobus fulgidus JCM 9628 | O29841 | - |
- |
| 2.7.1.160 | Archaeoglobus fulgidus NBRC 100126 | O29841 | - |
- |
| 2.7.1.160 | Archaeoglobus fulgidus VC-16 | O29841 | - |
- |
| 2.7.1.160 | Homo sapiens | Q86TN4 | - |
- |
| 2.7.1.160 | Pyrococcus horikoshii | O57899 | - |
- |
| 2.7.1.160 | Pyrococcus horikoshii ATCC 700860 | O57899 | - |
- |
| 2.7.1.160 | Pyrococcus horikoshii DSM 12428 | O57899 | - |
- |
| 2.7.1.160 | Pyrococcus horikoshii JCM 9974 | O57899 | - |
- |
| 2.7.1.160 | Pyrococcus horikoshii NBRC 100139 | O57899 | - |
- |
| 2.7.1.160 | Pyrococcus horikoshii OT-3 | O57899 | - |
- |
| 2.7.1.160 | Runella slithyformis | - |
- |
- |
| 2.7.1.160 | Saccharomyces cerevisiae | Q12272 | - |
- |
| 2.7.1.160 | Thermochaetoides thermophila | G0S5Z5 | - |
- |
| 2.7.1.160 | Thermochaetoides thermophila CBS 144.50 | G0S5Z5 | - |
- |
| 2.7.1.160 | Thermochaetoides thermophila DSM 1495 | G0S5Z5 | - |
- |
| 2.7.1.160 | Thermochaetoides thermophila IMI 039719 | G0S5Z5 | - |
- |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 2.7.1.160 | recombinant His6-tagged enzyme from Escherichia coli strain BL21(DE3) by nickel affinity chromatography | Homo sapiens |
| 2.7.1.160 | recombinant His6-tagged enzyme from Escherichia coli strain BL21(DE3) by nickel affinity chromatography | Acetivibrio thermocellus |
| 2.7.1.160 | recombinant His6-tagged enzyme from Escherichia coli strain BL21(DE3) by nickel affinity chromatography | Thermochaetoides thermophila |
| 2.7.1.160 | recombinant His6-tagged enzyme from Escherichia coli strain BL21(DE3) by nickel affinity chromatography | Archaeoglobus fulgidus |
| 2.7.1.160 | recombinant His6-tagged enzyme from Escherichia coli strain BL21(DE3) by nickel affinity chromatography and dialysis | Aeropyrum pernix |
| 2.7.1.160 | recombinant His6-tagged enzyme from Escherichia coli strain BL21(DE3) by nickel affinity chromatography and dialysis | Pyrococcus horikoshii |
| 2.7.1.160 | recombinant wild-type and mutant enzymes from Escherichia coli strain BL21(DE3) by nickel affinity chromatography | Runella slithyformis |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 2.7.1.160 | 2'-phospho-[ligated tRNA] + NAD+ | - |
Homo sapiens | mature tRNA + ADP-ribose 1'',2''-phosphate + nicotinamide | - |
? | |
| 2.7.1.160 | 2'-phospho-[ligated tRNA] + NAD+ | - |
Saccharomyces cerevisiae | mature tRNA + ADP-ribose 1'',2''-phosphate + nicotinamide | - |
? | |
| 2.7.1.160 | 2'-phospho-[ligated tRNA] + NAD+ | - |
Runella slithyformis | mature tRNA + ADP-ribose 1'',2''-phosphate + nicotinamide | - |
? | |
| 2.7.1.160 | 2'-phospho-[ligated tRNA] + NAD+ | - |
Acetivibrio thermocellus | mature tRNA + ADP-ribose 1'',2''-phosphate + nicotinamide | - |
? | |
| 2.7.1.160 | 2'-phospho-[ligated tRNA] + NAD+ | - |
Thermochaetoides thermophila | mature tRNA + ADP-ribose 1'',2''-phosphate + nicotinamide | - |
? | |
| 2.7.1.160 | 2'-phospho-[ligated tRNA] + NAD+ | - |
Aeropyrum pernix | mature tRNA + ADP-ribose 1'',2''-phosphate + nicotinamide | - |
? | |
| 2.7.1.160 | 2'-phospho-[ligated tRNA] + NAD+ | - |
Pyrococcus horikoshii | mature tRNA + ADP-ribose 1'',2''-phosphate + nicotinamide | - |
? | |
| 2.7.1.160 | 2'-phospho-[ligated tRNA] + NAD+ | - |
Archaeoglobus fulgidus | mature tRNA + ADP-ribose 1'',2''-phosphate + nicotinamide | - |
? | |
| 2.7.1.160 | 2'-phospho-[ligated tRNA] + NAD+ | 13% conversion by the Archaeoglobus fulgidus enzyme | Archaeoglobus fulgidus | mature tRNA + ADP-ribose 1'',2''-phosphate + nicotinamide | - |
? | |
| 2.7.1.160 | 2'-phospho-[ligated tRNA] + NAD+ | 20% conversion by the Pyrococcus horikoshii enzyme | Pyrococcus horikoshii | mature tRNA + ADP-ribose 1'',2''-phosphate + nicotinamide | - |
? | |
| 2.7.1.160 | 2'-phospho-[ligated tRNA] + NAD+ | 21% conversion by the Chaetomium thermophilum enzyme | Thermochaetoides thermophila | mature tRNA + ADP-ribose 1'',2''-phosphate + nicotinamide | - |
? | |
| 2.7.1.160 | 2'-phospho-[ligated tRNA] + NAD+ | 98% conversion by ApeTpt1 | Aeropyrum pernix | mature tRNA + ADP-ribose 1'',2''-phosphate + nicotinamide | - |
? | |
| 2.7.1.160 | 2'-phospho-[ligated tRNA] + NAD+ | - |
Aeropyrum pernix ATCC 700893 | mature tRNA + ADP-ribose 1'',2''-phosphate + nicotinamide | - |
? | |
| 2.7.1.160 | 2'-phospho-[ligated tRNA] + NAD+ | 98% conversion by ApeTpt1 | Aeropyrum pernix ATCC 700893 | mature tRNA + ADP-ribose 1'',2''-phosphate + nicotinamide | - |
? | |
| 2.7.1.160 | 2'-phospho-[ligated tRNA] + NAD+ | - |
Aeropyrum pernix DSM 11879 | mature tRNA + ADP-ribose 1'',2''-phosphate + nicotinamide | - |
? | |
| 2.7.1.160 | 2'-phospho-[ligated tRNA] + NAD+ | 98% conversion by ApeTpt1 | Aeropyrum pernix DSM 11879 | mature tRNA + ADP-ribose 1'',2''-phosphate + nicotinamide | - |
? | |
| 2.7.1.160 | 2'-phospho-[ligated tRNA] + NAD+ | - |
Thermochaetoides thermophila IMI 039719 | mature tRNA + ADP-ribose 1'',2''-phosphate + nicotinamide | - |
? | |
| 2.7.1.160 | 2'-phospho-[ligated tRNA] + NAD+ | 21% conversion by the Chaetomium thermophilum enzyme | Thermochaetoides thermophila IMI 039719 | mature tRNA + ADP-ribose 1'',2''-phosphate + nicotinamide | - |
? | |
| 2.7.1.160 | 2'-phospho-[ligated tRNA] + NAD+ | - |
Acetivibrio thermocellus DSM 1237 | mature tRNA + ADP-ribose 1'',2''-phosphate + nicotinamide | - |
? | |
| 2.7.1.160 | 2'-phospho-[ligated tRNA] + NAD+ | - |
Archaeoglobus fulgidus NBRC 100126 | mature tRNA + ADP-ribose 1'',2''-phosphate + nicotinamide | - |
? | |
| 2.7.1.160 | 2'-phospho-[ligated tRNA] + NAD+ | 13% conversion by the Archaeoglobus fulgidus enzyme | Archaeoglobus fulgidus NBRC 100126 | mature tRNA + ADP-ribose 1'',2''-phosphate + nicotinamide | - |
? | |
| 2.7.1.160 | 2'-phospho-[ligated tRNA] + NAD+ | - |
Archaeoglobus fulgidus ATCC 49558 | mature tRNA + ADP-ribose 1'',2''-phosphate + nicotinamide | - |
? | |
| 2.7.1.160 | 2'-phospho-[ligated tRNA] + NAD+ | 13% conversion by the Archaeoglobus fulgidus enzyme | Archaeoglobus fulgidus ATCC 49558 | mature tRNA + ADP-ribose 1'',2''-phosphate + nicotinamide | - |
? | |
| 2.7.1.160 | 2'-phospho-[ligated tRNA] + NAD+ | - |
Thermochaetoides thermophila DSM 1495 | mature tRNA + ADP-ribose 1'',2''-phosphate + nicotinamide | - |
? | |
| 2.7.1.160 | 2'-phospho-[ligated tRNA] + NAD+ | 21% conversion by the Chaetomium thermophilum enzyme | Thermochaetoides thermophila DSM 1495 | mature tRNA + ADP-ribose 1'',2''-phosphate + nicotinamide | - |
? | |
| 2.7.1.160 | 2'-phospho-[ligated tRNA] + NAD+ | - |
Pyrococcus horikoshii DSM 12428 | mature tRNA + ADP-ribose 1'',2''-phosphate + nicotinamide | - |
? | |
| 2.7.1.160 | 2'-phospho-[ligated tRNA] + NAD+ | 20% conversion by the Pyrococcus horikoshii enzyme | Pyrococcus horikoshii DSM 12428 | mature tRNA + ADP-ribose 1'',2''-phosphate + nicotinamide | - |
? | |
| 2.7.1.160 | 2'-phospho-[ligated tRNA] + NAD+ | - |
Acetivibrio thermocellus JCM 9322 | mature tRNA + ADP-ribose 1'',2''-phosphate + nicotinamide | - |
? | |
| 2.7.1.160 | 2'-phospho-[ligated tRNA] + NAD+ | - |
Acetivibrio thermocellus NBRC 103400 | mature tRNA + ADP-ribose 1'',2''-phosphate + nicotinamide | - |
? | |
| 2.7.1.160 | 2'-phospho-[ligated tRNA] + NAD+ | - |
Aeropyrum pernix JCM 9820 | mature tRNA + ADP-ribose 1'',2''-phosphate + nicotinamide | - |
? | |
| 2.7.1.160 | 2'-phospho-[ligated tRNA] + NAD+ | 98% conversion by ApeTpt1 | Aeropyrum pernix JCM 9820 | mature tRNA + ADP-ribose 1'',2''-phosphate + nicotinamide | - |
? | |
| 2.7.1.160 | 2'-phospho-[ligated tRNA] + NAD+ | - |
Pyrococcus horikoshii NBRC 100139 | mature tRNA + ADP-ribose 1'',2''-phosphate + nicotinamide | - |
? | |
| 2.7.1.160 | 2'-phospho-[ligated tRNA] + NAD+ | 20% conversion by the Pyrococcus horikoshii enzyme | Pyrococcus horikoshii NBRC 100139 | mature tRNA + ADP-ribose 1'',2''-phosphate + nicotinamide | - |
? | |
| 2.7.1.160 | 2'-phospho-[ligated tRNA] + NAD+ | - |
Pyrococcus horikoshii JCM 9974 | mature tRNA + ADP-ribose 1'',2''-phosphate + nicotinamide | - |
? | |
| 2.7.1.160 | 2'-phospho-[ligated tRNA] + NAD+ | 20% conversion by the Pyrococcus horikoshii enzyme | Pyrococcus horikoshii JCM 9974 | mature tRNA + ADP-ribose 1'',2''-phosphate + nicotinamide | - |
? | |
| 2.7.1.160 | 2'-phospho-[ligated tRNA] + NAD+ | - |
Acetivibrio thermocellus ATCC 27405 | mature tRNA + ADP-ribose 1'',2''-phosphate + nicotinamide | - |
? | |
| 2.7.1.160 | 2'-phospho-[ligated tRNA] + NAD+ | - |
Acetivibrio thermocellus VPI 7372 | mature tRNA + ADP-ribose 1'',2''-phosphate + nicotinamide | - |
? | |
| 2.7.1.160 | 2'-phospho-[ligated tRNA] + NAD+ | - |
Archaeoglobus fulgidus VC-16 | mature tRNA + ADP-ribose 1'',2''-phosphate + nicotinamide | - |
? | |
| 2.7.1.160 | 2'-phospho-[ligated tRNA] + NAD+ | 13% conversion by the Archaeoglobus fulgidus enzyme | Archaeoglobus fulgidus VC-16 | mature tRNA + ADP-ribose 1'',2''-phosphate + nicotinamide | - |
? | |
| 2.7.1.160 | 2'-phospho-[ligated tRNA] + NAD+ | - |
Thermochaetoides thermophila CBS 144.50 | mature tRNA + ADP-ribose 1'',2''-phosphate + nicotinamide | - |
? | |
| 2.7.1.160 | 2'-phospho-[ligated tRNA] + NAD+ | 21% conversion by the Chaetomium thermophilum enzyme | Thermochaetoides thermophila CBS 144.50 | mature tRNA + ADP-ribose 1'',2''-phosphate + nicotinamide | - |
? | |
| 2.7.1.160 | 2'-phospho-[ligated tRNA] + NAD+ | - |
Aeropyrum pernix NBRC 100138 | mature tRNA + ADP-ribose 1'',2''-phosphate + nicotinamide | - |
? | |
| 2.7.1.160 | 2'-phospho-[ligated tRNA] + NAD+ | 98% conversion by ApeTpt1 | Aeropyrum pernix NBRC 100138 | mature tRNA + ADP-ribose 1'',2''-phosphate + nicotinamide | - |
? | |
| 2.7.1.160 | 2'-phospho-[ligated tRNA] + NAD+ | - |
Pyrococcus horikoshii ATCC 700860 | mature tRNA + ADP-ribose 1'',2''-phosphate + nicotinamide | - |
? | |
| 2.7.1.160 | 2'-phospho-[ligated tRNA] + NAD+ | 20% conversion by the Pyrococcus horikoshii enzyme | Pyrococcus horikoshii ATCC 700860 | mature tRNA + ADP-ribose 1'',2''-phosphate + nicotinamide | - |
? | |
| 2.7.1.160 | 2'-phospho-[ligated tRNA] + NAD+ | - |
Acetivibrio thermocellus NCIMB 10682 | mature tRNA + ADP-ribose 1'',2''-phosphate + nicotinamide | - |
? | |
| 2.7.1.160 | 2'-phospho-[ligated tRNA] + NAD+ | - |
Pyrococcus horikoshii OT-3 | mature tRNA + ADP-ribose 1'',2''-phosphate + nicotinamide | - |
? | |
| 2.7.1.160 | 2'-phospho-[ligated tRNA] + NAD+ | 20% conversion by the Pyrococcus horikoshii enzyme | Pyrococcus horikoshii OT-3 | mature tRNA + ADP-ribose 1'',2''-phosphate + nicotinamide | - |
? | |
| 2.7.1.160 | 2'-phospho-[ligated tRNA] + NAD+ | - |
Acetivibrio thermocellus NRRL B-4536 | mature tRNA + ADP-ribose 1'',2''-phosphate + nicotinamide | - |
? | |
| 2.7.1.160 | 2'-phospho-[ligated tRNA] + NAD+ | - |
Archaeoglobus fulgidus JCM 9628 | mature tRNA + ADP-ribose 1'',2''-phosphate + nicotinamide | - |
? | |
| 2.7.1.160 | 2'-phospho-[ligated tRNA] + NAD+ | 13% conversion by the Archaeoglobus fulgidus enzyme | Archaeoglobus fulgidus JCM 9628 | mature tRNA + ADP-ribose 1'',2''-phosphate + nicotinamide | - |
? | |
| 2.7.1.160 | additional information | enzyme ApeTpt1 catalyzes a one-step synthesis of a 5'-phospho-ADP-ribosylated cap structure (with no subsequent 5'-phosphotransferase step). ApeTpt1 caps RNA or DNA 5'-phosphate ends with ADP-ribose. pDNA and pRNA substrates are capped quantitatively at saturating enzyme in preence of NAD+. ApeTpt1 with 200 nM 10mer 5' 32P-labeled RNA or DNA oligonucleotides of identical nucleotide sequence (excepting T for U in DNA versus RNA) in the presence of 1 mM NAD+ resulted in the conversion of the substrates into RNA and DNA products of slower electrophoretic mobility. The 32P-labeled capped oligonucleotides are gel-purified and reacted with ApeTpt1 in the presence of 1 mM nicotinamide. This results in decapping of 20% of the ADPR-pDNA substrate to regenerate the pDNA strand | Aeropyrum pernix | ? | - |
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| 2.7.1.160 | additional information | enzyme ApeTpt1 catalyzes a one-step synthesis of a 5'-phospho-ADP-ribosylated cap structure (with no subsequent 5'-phosphotransferase step). ApeTpt1 caps RNA or DNA 5'-phosphate ends with ADP-ribose. pDNA and pRNA substrates are capped quantitatively at saturating enzyme in preence of NAD+. ApeTpt1 with 200 nM 10mer 5' 32P-labeled RNA or DNA oligonucleotides of identical nucleotide sequence (excepting T for U in DNA versus RNA) in the presence of 1 mM NAD+ resulted in the conversion of the substrates into RNA and DNA products of slower electrophoretic mobility. The 32P-labeled capped oligonucleotides are gel-purified and reacted with ApeTpt1 in the presence of 1 mM nicotinamide. This results in decapping of 20% of the ADPR-pDNA substrate to regenerate the pDNA strand | Aeropyrum pernix ATCC 700893 | ? | - |
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| 2.7.1.160 | additional information | enzyme ApeTpt1 catalyzes a one-step synthesis of a 5'-phospho-ADP-ribosylated cap structure (with no subsequent 5'-phosphotransferase step). ApeTpt1 caps RNA or DNA 5'-phosphate ends with ADP-ribose. pDNA and pRNA substrates are capped quantitatively at saturating enzyme in preence of NAD+. ApeTpt1 with 200 nM 10mer 5' 32P-labeled RNA or DNA oligonucleotides of identical nucleotide sequence (excepting T for U in DNA versus RNA) in the presence of 1 mM NAD+ resulted in the conversion of the substrates into RNA and DNA products of slower electrophoretic mobility. The 32P-labeled capped oligonucleotides are gel-purified and reacted with ApeTpt1 in the presence of 1 mM nicotinamide. This results in decapping of 20% of the ADPR-pDNA substrate to regenerate the pDNA strand | Aeropyrum pernix DSM 11879 | ? | - |
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| 2.7.1.160 | additional information | enzyme ApeTpt1 catalyzes a one-step synthesis of a 5'-phospho-ADP-ribosylated cap structure (with no subsequent 5'-phosphotransferase step). ApeTpt1 caps RNA or DNA 5'-phosphate ends with ADP-ribose. pDNA and pRNA substrates are capped quantitatively at saturating enzyme in preence of NAD+. ApeTpt1 with 200 nM 10mer 5' 32P-labeled RNA or DNA oligonucleotides of identical nucleotide sequence (excepting T for U in DNA versus RNA) in the presence of 1 mM NAD+ resulted in the conversion of the substrates into RNA and DNA products of slower electrophoretic mobility. The 32P-labeled capped oligonucleotides are gel-purified and reacted with ApeTpt1 in the presence of 1 mM nicotinamide. This results in decapping of 20% of the ADPR-pDNA substrate to regenerate the pDNA strand | Aeropyrum pernix JCM 9820 | ? | - |
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| 2.7.1.160 | additional information | enzyme ApeTpt1 catalyzes a one-step synthesis of a 5'-phospho-ADP-ribosylated cap structure (with no subsequent 5'-phosphotransferase step). ApeTpt1 caps RNA or DNA 5'-phosphate ends with ADP-ribose. pDNA and pRNA substrates are capped quantitatively at saturating enzyme in preence of NAD+. ApeTpt1 with 200 nM 10mer 5' 32P-labeled RNA or DNA oligonucleotides of identical nucleotide sequence (excepting T for U in DNA versus RNA) in the presence of 1 mM NAD+ resulted in the conversion of the substrates into RNA and DNA products of slower electrophoretic mobility. The 32P-labeled capped oligonucleotides are gel-purified and reacted with ApeTpt1 in the presence of 1 mM nicotinamide. This results in decapping of 20% of the ADPR-pDNA substrate to regenerate the pDNA strand | Aeropyrum pernix NBRC 100138 | ? | - |
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Synonyms
| EC Number | Synonyms | Comment | Organism |
|---|---|---|---|
| 2.7.1.160 | ApeTpt1 | - |
Aeropyrum pernix |
| 2.7.1.160 | CTHT_0025470 | - |
Thermochaetoides thermophila |
| 2.7.1.160 | KptA | - |
Acetivibrio thermocellus |
| 2.7.1.160 | KptA | - |
Aeropyrum pernix |
| 2.7.1.160 | KptA | - |
Pyrococcus horikoshii |
| 2.7.1.160 | kptA1 | - |
Archaeoglobus fulgidus |
| 2.7.1.160 | RNA 2'-phosphotransferase | - |
Homo sapiens |
| 2.7.1.160 | RNA 2'-phosphotransferase | - |
Saccharomyces cerevisiae |
| 2.7.1.160 | RNA 2'-phosphotransferase | - |
Runella slithyformis |
| 2.7.1.160 | RNA 2'-phosphotransferase | - |
Acetivibrio thermocellus |
| 2.7.1.160 | RNA 2'-phosphotransferase | - |
Thermochaetoides thermophila |
| 2.7.1.160 | RNA 2'-phosphotransferase | - |
Aeropyrum pernix |
| 2.7.1.160 | RNA 2'-phosphotransferase | - |
Pyrococcus horikoshii |
| 2.7.1.160 | RNA 2'-phosphotransferase | - |
Archaeoglobus fulgidus |
| 2.7.1.160 | RslTpt1 | - |
Runella slithyformis |
| 2.7.1.160 | Tpt1 | - |
Homo sapiens |
| 2.7.1.160 | Tpt1 | - |
Saccharomyces cerevisiae |
| 2.7.1.160 | Tpt1 | - |
Runella slithyformis |
| 2.7.1.160 | Tpt1 | - |
Acetivibrio thermocellus |
| 2.7.1.160 | Tpt1 | - |
Thermochaetoides thermophila |
| 2.7.1.160 | Tpt1 | - |
Aeropyrum pernix |
| 2.7.1.160 | Tpt1 | - |
Pyrococcus horikoshii |
| 2.7.1.160 | Tpt1 | - |
Archaeoglobus fulgidus |
Temperature Optimum [°C]
| EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 2.7.1.160 | 37 | - |
assay at | Homo sapiens |
| 2.7.1.160 | 37 | - |
assay at | Saccharomyces cerevisiae |
| 2.7.1.160 | 37 | - |
assay at | Runella slithyformis |
| 2.7.1.160 | 37 | - |
assay at | Acetivibrio thermocellus |
| 2.7.1.160 | 37 | - |
assay at | Thermochaetoides thermophila |
| 2.7.1.160 | 37 | - |
assay at | Aeropyrum pernix |
| 2.7.1.160 | 37 | - |
assay at | Pyrococcus horikoshii |
| 2.7.1.160 | 37 | - |
assay at | Archaeoglobus fulgidus |
Temperature Range [°C]
| EC Number | Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 2.7.1.160 | 37 | 95 | ApeTpt1 caps the pDNA substrate with high yield (94-100%) at temperatures ranging from 37°C to 65°C and with progressively lower yields at 75°C (68%), 85°C (32%), and 95°C (21%) | Aeropyrum pernix |
pH Optimum
| EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|---|
| 2.7.1.160 | 7.5 | - |
assay at | Homo sapiens |
| 2.7.1.160 | 7.5 | - |
assay at | Saccharomyces cerevisiae |
| 2.7.1.160 | 7.5 | - |
assay at | Runella slithyformis |
| 2.7.1.160 | 7.5 | - |
assay at | Acetivibrio thermocellus |
| 2.7.1.160 | 7.5 | - |
assay at | Thermochaetoides thermophila |
| 2.7.1.160 | 7.5 | - |
assay at | Aeropyrum pernix |
| 2.7.1.160 | 7.5 | - |
assay at | Pyrococcus horikoshii |
| 2.7.1.160 | 7.5 | - |
assay at | Archaeoglobus fulgidus |
Cofactor
| EC Number | Cofactor | Comment | Organism | Structure |
|---|---|---|---|---|
| 2.7.1.160 | NAD+ | - |
Homo sapiens | |
| 2.7.1.160 | NAD+ | - |
Saccharomyces cerevisiae | |
| 2.7.1.160 | NAD+ | - |
Runella slithyformis | |
| 2.7.1.160 | NAD+ | - |
Acetivibrio thermocellus | |
| 2.7.1.160 | NAD+ | - |
Thermochaetoides thermophila | |
| 2.7.1.160 | NAD+ | - |
Aeropyrum pernix | |
| 2.7.1.160 | NAD+ | - |
Pyrococcus horikoshii | |
| 2.7.1.160 | NAD+ | - |
Archaeoglobus fulgidus | |
General Information
| EC Number | General Information | Comment | Organism |
|---|---|---|---|
| 2.7.1.160 | evolution | wide distribution of Tpt1 enzymes in taxa that have no fungal-type RNA ligase. Whereas all of the Tpt1 enzymes are capable of NAD+-dependent conversion of an internal RNA 2'-PO4 to a 2'-OH (the canonical Tpt1 reaction), a subset of Tpt1 enzymes also catalyze NAD+-dependent ADP-ribosylation of an RNA or DNA 5'-phosphate terminus | Homo sapiens |
| 2.7.1.160 | evolution | wide distribution of Tpt1 enzymes in taxa that have no fungal-type RNA ligase. Whereas all of the Tpt1 enzymes are capable of NAD+-dependent conversion of an internal RNA 2'-PO4 to a 2'-OH (the canonical Tpt1 reaction), a subset of Tpt1 enzymes also catalyze NAD+-dependent ADP-ribosylation of an RNA or DNA 5'-phosphate terminus | Saccharomyces cerevisiae |
| 2.7.1.160 | evolution | wide distribution of Tpt1 enzymes in taxa that have no fungal-type RNA ligase. Whereas all of the Tpt1 enzymes are capable of NAD+-dependent conversion of an internal RNA 2'-PO4 to a 2'-OH (the canonical Tpt1 reaction), a subset of Tpt1 enzymes also catalyze NAD+-dependent ADP-ribosylation of an RNA or DNA 5'-phosphate terminus | Runella slithyformis |
| 2.7.1.160 | evolution | wide distribution of Tpt1 enzymes in taxa that have no fungal-type RNA ligase. Whereas all of the Tpt1 enzymes are capable of NAD+-dependent conversion of an internal RNA 2'-PO4 to a 2'-OH (the canonical Tpt1 reaction), a subset of Tpt1 enzymes also catalyze NAD+-dependent ADP-ribosylation of an RNA or DNA 5'-phosphate terminus | Acetivibrio thermocellus |
| 2.7.1.160 | evolution | wide distribution of Tpt1 enzymes in taxa that have no fungal-type RNA ligase. Whereas all of the Tpt1 enzymes are capable of NAD+-dependent conversion of an internal RNA 2'-PO4 to a 2'-OH (the canonical Tpt1 reaction), a subset of Tpt1 enzymes also catalyze NAD+-dependent ADP-ribosylation of an RNA or DNA 5'-phosphate terminus | Thermochaetoides thermophila |
| 2.7.1.160 | evolution | wide distribution of Tpt1 enzymes in taxa that have no fungal-type RNA ligase. Whereas all of the Tpt1 enzymes are capable of NAD+-dependent conversion of an internal RNA 2'-PO4 to a 2'-OH (the canonical Tpt1 reaction), a subset of Tpt1 enzymes also catalyze NAD+-dependent ADP-ribosylation of an RNA or DNA 5'-phosphate terminus | Pyrococcus horikoshii |
| 2.7.1.160 | evolution | wide distribution of Tpt1 enzymes in taxa that have no fungal-type RNA ligase. Whereas all of the Tpt1 enzymes are capable of NAD+-dependent conversion of an internal RNA 2'-PO4 to a 2'-OH (the canonical Tpt1 reaction), a subset of Tpt1 enzymes also catalyze NAD+-dependent ADP-ribosylation of an RNA or DNA 5'-phosphate terminus | Archaeoglobus fulgidus |
| 2.7.1.160 | evolution | wide distribution of Tpt1 enzymes in taxa that have no fungal-type RNA ligase. Whereas all of the Tpt1 enzymes are capable of NAD+-dependent conversion of an internal RNA 2'-PO4 to a 2'-OH (the canonical Tpt1 reaction), a subset of Tpt1 enzymes also catalyze NAD+-dependent ADP-ribosylation of an RNA or DNA 5'-phosphate terminus. Aeropyrum pernix Tpt1 (ApeTpt1) is particularly adept in this respect | Aeropyrum pernix |
| 2.7.1.160 | physiological function | the RNA 2'-phosphotransferase Tpt1 converts an internal RNA 2'-phosphate to a 2'-OH via a two-step NAD+-dependent mechanism in which: (i) the 2'-phosphate attacks the C1'' of NAD+ to expel nicotinamide and form a 2'-phospho-ADP-ribosylated RNA intermediate, and (ii) the ADP-ribose O2'' attacks the phosphate of the RNA 2'-phospho-ADPR intermediate to expel the RNA 2'-OH and generate ADP-ribose 1''-2'' cyclic phosphate. Tpt1 is an essential component of the fungal tRNA splicing pathway that generates a unique 2'-phosphate, 3'-5' phosphodiester splice junction during tRNA ligation. Tpt1 can catalyze reactions other than RNA 2'-phosphate removal. Whereas all of the Tpt1 enzymes are capable of NAD+-dependent conversion of an internal RNA 2'-phosphate to a 2'-hydroxy (the canonical Tpt1 reaction), a subset of Tpt1 enzymes also catalyze NAD+-dependent ADP-ribosylation of an RNA or DNA 5'-phosphate terminus. Aeropyrum pernix Tpt1 (ApeTpt1) is particularly adept in this respect | Aeropyrum pernix |
| 2.7.1.160 | physiological function | the RNA 2'-phosphotransferase Tpt1 converts an internal RNA 2'-phosphate to a 2'-OH via a two-step NAD+-dependent mechanism in which: (i) the 2'-phosphate attacks the C1'' of NAD+ to expel nicotinamide and form a 2'-phospho-ADP-ribosylated RNA intermediate, and (ii) the ADP-ribose O2'' attacks the phosphate of the RNA 2'-phospho-ADPR intermediate to expel the RNA 2'-OH and generate ADP-ribose 1''-2'' cyclic phosphate. Tpt1 is an essential component of the fungal tRNA splicing pathway that generates a unique 2'-phosphate, 3'-5'-phosphodiester splice junction during tRNA ligation. Tpt1 can catalyze reactions other than RNA 2'-phosphate removal. Whereas all of the Tpt1 enzymes are capable of NAD+-dependent conversion of an internal RNA 2'-phosphate to a 2'-hydroxy (the canonical Tpt1 reaction), a subset of Tpt1 enzymes also catalyze NAD+-dependent ADP-ribosylation of an RNA or DNA 5'-phosphate terminus | Homo sapiens |
| 2.7.1.160 | physiological function | the RNA 2'-phosphotransferase Tpt1 converts an internal RNA 2'-phosphate to a 2'-OH via a two-step NAD+-dependent mechanism in which: (i) the 2'-phosphate attacks the C1'' of NAD+ to expel nicotinamide and form a 2'-phospho-ADP-ribosylated RNA intermediate, and (ii) the ADP-ribose O2'' attacks the phosphate of the RNA 2'-phospho-ADPR intermediate to expel the RNA 2'-OH and generate ADP-ribose 1''-2'' cyclic phosphate. Tpt1 is an essential component of the fungal tRNA splicing pathway that generates a unique 2'-phosphate, 3'-5'-phosphodiester splice junction during tRNA ligation. Tpt1 can catalyze reactions other than RNA 2'-phosphate removal. Whereas all of the Tpt1 enzymes are capable of NAD+-dependent conversion of an internal RNA 2'-phosphate to a 2'-hydroxy (the canonical Tpt1 reaction), a subset of Tpt1 enzymes also catalyze NAD+-dependent ADP-ribosylation of an RNA or DNA 5'-phosphate terminus | Saccharomyces cerevisiae |
| 2.7.1.160 | physiological function | the RNA 2'-phosphotransferase Tpt1 converts an internal RNA 2'-phosphate to a 2'-OH via a two-step NAD+-dependent mechanism in which: (i) the 2'-phosphate attacks the C1'' of NAD+ to expel nicotinamide and form a 2'-phospho-ADP-ribosylated RNA intermediate, and (ii) the ADP-ribose O2'' attacks the phosphate of the RNA 2'-phospho-ADPR intermediate to expel the RNA 2'-OH and generate ADP-ribose 1''-2'' cyclic phosphate. Tpt1 is an essential component of the fungal tRNA splicing pathway that generates a unique 2'-phosphate, 3'-5'-phosphodiester splice junction during tRNA ligation. Tpt1 can catalyze reactions other than RNA 2'-phosphate removal. Whereas all of the Tpt1 enzymes are capable of NAD+-dependent conversion of an internal RNA 2'-phosphate to a 2'-hydroxy (the canonical Tpt1 reaction), a subset of Tpt1 enzymes also catalyze NAD+-dependent ADP-ribosylation of an RNA or DNA 5'-phosphate terminus | Runella slithyformis |
| 2.7.1.160 | physiological function | the RNA 2'-phosphotransferase Tpt1 converts an internal RNA 2'-phosphate to a 2'-OH via a two-step NAD+-dependent mechanism in which: (i) the 2'-phosphate attacks the C1'' of NAD+ to expel nicotinamide and form a 2'-phospho-ADP-ribosylated RNA intermediate, and (ii) the ADP-ribose O2'' attacks the phosphate of the RNA 2'-phospho-ADPR intermediate to expel the RNA 2'-OH and generate ADP-ribose 1''-2'' cyclic phosphate. Tpt1 is an essential component of the fungal tRNA splicing pathway that generates a unique 2'-phosphate, 3'-5'-phosphodiester splice junction during tRNA ligation. Tpt1 can catalyze reactions other than RNA 2'-phosphate removal. Whereas all of the Tpt1 enzymes are capable of NAD+-dependent conversion of an internal RNA 2'-phosphate to a 2'-hydroxy (the canonical Tpt1 reaction), a subset of Tpt1 enzymes also catalyze NAD+-dependent ADP-ribosylation of an RNA or DNA 5'-phosphate terminus | Acetivibrio thermocellus |
| 2.7.1.160 | physiological function | the RNA 2'-phosphotransferase Tpt1 converts an internal RNA 2'-phosphate to a 2'-OH via a two-step NAD+-dependent mechanism in which: (i) the 2'-phosphate attacks the C1'' of NAD+ to expel nicotinamide and form a 2'-phospho-ADP-ribosylated RNA intermediate, and (ii) the ADP-ribose O2'' attacks the phosphate of the RNA 2'-phospho-ADPR intermediate to expel the RNA 2'-OH and generate ADP-ribose 1''-2'' cyclic phosphate. Tpt1 is an essential component of the fungal tRNA splicing pathway that generates a unique 2'-phosphate, 3'-5'-phosphodiester splice junction during tRNA ligation. Tpt1 can catalyze reactions other than RNA 2'-phosphate removal. Whereas all of the Tpt1 enzymes are capable of NAD+-dependent conversion of an internal RNA 2'-phosphate to a 2'-hydroxy (the canonical Tpt1 reaction), a subset of Tpt1 enzymes also catalyze NAD+-dependent ADP-ribosylation of an RNA or DNA 5'-phosphate terminus | Thermochaetoides thermophila |
| 2.7.1.160 | physiological function | the RNA 2'-phosphotransferase Tpt1 converts an internal RNA 2'-phosphate to a 2'-OH via a two-step NAD+-dependent mechanism in which: (i) the 2'-phosphate attacks the C1'' of NAD+ to expel nicotinamide and form a 2'-phospho-ADP-ribosylated RNA intermediate, and (ii) the ADP-ribose O2'' attacks the phosphate of the RNA 2'-phospho-ADPR intermediate to expel the RNA 2'-OH and generate ADP-ribose 1''-2'' cyclic phosphate. Tpt1 is an essential component of the fungal tRNA splicing pathway that generates a unique 2'-phosphate, 3'-5'-phosphodiester splice junction during tRNA ligation. Tpt1 can catalyze reactions other than RNA 2'-phosphate removal. Whereas all of the Tpt1 enzymes are capable of NAD+-dependent conversion of an internal RNA 2'-phosphate to a 2'-hydroxy (the canonical Tpt1 reaction), a subset of Tpt1 enzymes also catalyze NAD+-dependent ADP-ribosylation of an RNA or DNA 5'-phosphate terminus | Pyrococcus horikoshii |
| 2.7.1.160 | physiological function | the RNA 2'-phosphotransferase Tpt1 converts an internal RNA 2'-phosphate to a 2'-OH via a two-step NAD+-dependent mechanism in which: (i) the 2'-phosphate attacks the C1'' of NAD+ to expel nicotinamide and form a 2'-phospho-ADP-ribosylated RNA intermediate, and (ii) the ADP-ribose O2'' attacks the phosphate of the RNA 2'-phospho-ADPR intermediate to expel the RNA 2'-OH and generate ADP-ribose 1''-2'' cyclic phosphate. Tpt1 is an essential component of the fungal tRNA splicing pathway that generates a unique 2'-phosphate, 3'-5'-phosphodiester splice junction during tRNA ligation. Tpt1 can catalyze reactions other than RNA 2'-phosphate removal. Whereas all of the Tpt1 enzymes are capable of NAD+-dependent conversion of an internal RNA 2'-phosphate to a 2'-hydroxy (the canonical Tpt1 reaction), a subset of Tpt1 enzymes also catalyze NAD+-dependent ADP-ribosylation of an RNA or DNA 5'-phosphate terminus | Archaeoglobus fulgidus |
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