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Literature summary extracted from

  • Guo, J.; Li, L.; Zhou, S.; Su, Y.; Li, X.; Sun, J.; Ge, R.S.
    Butylated hydroxyanisole alters rat 5alpha-reductase and 3alpha-hydroxysteroid dehydrogenase Implications for influences of neurosteroidogenesis (2017), Neurosci. Lett., 653, 132-138 .
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.1.1.50 expressed in COS1 cells Rattus norvegicus
1.3.1.22 expressed in COS1 cells Rattus norvegicus

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.1.1.50 tert-butyl-4-hydroxyanisole a mixture of two isomers of tert-butyl-4-hydroxyanisole. Competitive inhibitor Rattus norvegicus
1.3.1.22 tert-butyl-4-hydroxyanisole a mixture of two isomers of tert-butyl-4-hydroxyanisole. Competitive inhibitor Rattus norvegicus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.1.1.50 0.00315
-
 dihydrotestosterone 37°C, pH not specified in the publication Rattus norvegicus
1.3.1.22 0.0014
-
 dihydrotestosterone 37°C, pH not specified in the publication Rattus norvegicus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.1.1.50 dihydrotestosterone + NADPH Rattus norvegicus
-
 androstanediol + NADP+ + H+
-
 ?
1.3.1.22 testosterone + NADPH + H+ Rattus norvegicus
-
 dihydrotestosterone + NADP+
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.1.1.50 Rattus norvegicus P23457
-
-
1.3.1.22 Rattus norvegicus P24008
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.1.50 dihydrotestosterone + NADPH
-
 Rattus norvegicus androstanediol + NADP+ + H+
-
 ?
1.3.1.22 testosterone + NADPH + H+
-
 Rattus norvegicus dihydrotestosterone + NADP+
-
 ?

Synonyms

EC Number Synonyms Comment Organism
1.1.1.50 3alpha-hydroxysteroid dehydrogenase
-
 Rattus norvegicus
1.1.1.50 AKR1C14
-
 Rattus norvegicus
1.1.1.50 AKR1C9
-
 Rattus norvegicus
1.3.1.22 brainneurosteroidogenic 5x02-reductase 1
-
 Rattus norvegicus
1.3.1.22 SRD5A1
-
 Rattus norvegicus

IC50 Value

EC Number IC50 Value IC50 Value Maximum Comment Organism Inhibitor Structure
1.1.1.50 0.0058
-
 37°C, pH not specified in the publication Rattus norvegicus tert-butyl-4-hydroxyanisole
1.3.1.22 0.0047
-
 37°C, pH not specified in the publication Rattus norvegicus tert-butyl-4-hydroxyanisole