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Literature summary extracted from

  • Moore, T.; Escalante-Semerena, J.
    The EutQ and EutP proteins are novel acetate kinases involved in ethanolamine catabolism Physiological implications for the function of the ethanolamine metabolosome in Salmonella enterica (2016), Mol. Microbiol., 99, 497-511 .
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.7.2.1 expressed in Escherichia coli BL21(lambdaDE3) cells Salmonella enterica subsp. enterica serovar Typhimurium

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.7.2.1 0.5
-
 ATP acetate kinase EutP, at 40°C, pH not specified in the publication Salmonella enterica subsp. enterica serovar Typhimurium
2.7.2.1 0.5
-
 ATP acetate kinase EutQ, at 40°C, pH not specified in the publication Salmonella enterica subsp. enterica serovar Typhimurium
2.7.2.1 0.7
-
 acetate acetate kinase EutP, at 40°C, pH not specified in the publication Salmonella enterica subsp. enterica serovar Typhimurium
2.7.2.1 0.7
-
 acetate acetate kinase EutQ, at 40°C, pH not specified in the publication Salmonella enterica subsp. enterica serovar Typhimurium

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.7.2.1 Mg2+ required for activity Salmonella enterica subsp. enterica serovar Typhimurium

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.7.2.1 ADP + acetyl phosphate Salmonella enterica subsp. enterica serovar Typhimurium
-
 ATP + acetate
-
 r
2.7.2.1 ATP + acetate Salmonella enterica subsp. enterica serovar Typhimurium
-
 ADP + acetyl phosphate
-
 r

Organism

EC Number Organism UniProt Comment Textmining
2.7.2.1 Salmonella enterica subsp. enterica serovar Typhimurium
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.7.2.1 HisTrap column chromatography Salmonella enterica subsp. enterica serovar Typhimurium

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.7.2.1 1750
-
 acetate kinase EutP, with GTP as cosubstrate, at 40°C, pH not specified in the publication Salmonella enterica subsp. enterica serovar Typhimurium
2.7.2.1 2400
-
 acetate kinase EutP, with ATP as cosubstrate, at 40°C, pH not specified in the publication Salmonella enterica subsp. enterica serovar Typhimurium

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.2.1 ADP + acetyl phosphate
-
 Salmonella enterica subsp. enterica serovar Typhimurium ATP + acetate
-
 r
2.7.2.1 ATP + acetate
-
 Salmonella enterica subsp. enterica serovar Typhimurium ADP + acetyl phosphate
-
 r
2.7.2.1 ATP + acetate highest activity Salmonella enterica subsp. enterica serovar Typhimurium ADP + acetyl phosphate
-
 r
2.7.2.1 GTP + acetate
-
 Salmonella enterica subsp. enterica serovar Typhimurium GDP + acetyl phosphate
-
 r
2.7.2.1 additional information no activity with AMP-PCP, CTP or TTP Salmonella enterica subsp. enterica serovar Typhimurium ?
-
-

Synonyms

EC Number Synonyms Comment Organism
2.7.2.1 EutP
-
 Salmonella enterica subsp. enterica serovar Typhimurium
2.7.2.1 EutQ
-
 Salmonella enterica subsp. enterica serovar Typhimurium

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2.7.2.1 318
-
 acetate acetate kinase EutQ, at 40°C, pH not specified in the publication Salmonella enterica subsp. enterica serovar Typhimurium
2.7.2.1 434
-
 ATP acetate kinase EutQ, at 40°C, pH not specified in the publication Salmonella enterica subsp. enterica serovar Typhimurium
2.7.2.1 545
-
 acetate acetate kinase EutP, at 40°C, pH not specified in the publication Salmonella enterica subsp. enterica serovar Typhimurium
2.7.2.1 657
-
 ATP acetate kinase EutP, at 40°C, pH not specified in the publication Salmonella enterica subsp. enterica serovar Typhimurium

General Information

EC Number General Information Comment Organism
2.7.2.1 physiological function acetate kinase EutQ is required during anoxic growth of Salmonella enterica on ethanolamine and tetrathionate Salmonella enterica subsp. enterica serovar Typhimurium

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
2.7.2.1 454
-
 acetate acetate kinase EutQ, at 40°C, pH not specified in the publication Salmonella enterica subsp. enterica serovar Typhimurium
2.7.2.1 778
-
 acetate acetate kinase EutP, at 40°C, pH not specified in the publication Salmonella enterica subsp. enterica serovar Typhimurium
2.7.2.1 868
-
 ATP acetate kinase EutQ, at 40°C, pH not specified in the publication Salmonella enterica subsp. enterica serovar Typhimurium
2.7.2.1 1314
-
 ATP acetate kinase EutP, at 40°C, pH not specified in the publication Salmonella enterica subsp. enterica serovar Typhimurium