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Literature summary extracted from

  • Giordana, L.; Nowicki, C.
    Two phylogenetically divergent isocitrate dehydrogenases are encoded in Leishmania parasites. Molecular and functional characterization of Leishmania mexicana isoenzymes with specificity towards NAD+ and NADP+ (2020), Mol. Biochem. Parasitol., 240, 111320 .
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.1.1.41 expressed in Escherichia coli BL21-CodonPlus (DE3) RIL cells Leishmania mexicana
1.1.1.42 expressed in Escherichia coli BL21-CodonPlus (DE3) RIL cells Leishmania mexicana

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.1.1.41 0.002
-
 DL-isocitrate at pH 7.4 and 37°C Leishmania mexicana
1.1.1.41 0.05
-
 NAD+ at pH 7.4 and 37°C Leishmania mexicana
1.1.1.42 0.015
-
 NADP+ at pH 7.4 and 37°C Leishmania mexicana
1.1.1.42 0.043
-
 DL-isocitrate at pH 7.4 and 37°C Leishmania mexicana

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.1.1.41 Mn2+ Km of 0.002 mM, 0.5 mM used in assay conditions Leishmania mexicana
1.1.1.42 Mn2+ Km of 0.002 mM, 0.5 mM used in assay conditions Leishmania mexicana

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.1.1.41 DL-isocitrate + NAD+ Leishmania mexicana
-
 2-oxoglutarate + CO2 + NADH + H+
-
 ?
1.1.1.42 DL-isocitrate + NADP+ Leishmania mexicana
-
 2-oxoglutarate + CO2 + NADPH + H+
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.1.1.41 Leishmania mexicana
-
-
-
1.1.1.42 Leishmania mexicana
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.1.1.41 Ni-NTA column chromatography and Sephacryl-S200 gel filtration Leishmania mexicana
1.1.1.42 Ni-NTA column chromatography and Sephacryl-S200 gel filtration Leishmania mexicana

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.1.1.41 promastigote
-
 Leishmania mexicana
-
1.1.1.42 promastigote
-
 Leishmania mexicana
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.1.41 DL-isocitrate + NAD+
-
 Leishmania mexicana 2-oxoglutarate + CO2 + NADH + H+
-
 ?
1.1.1.42 DL-isocitrate + NADP+
-
 Leishmania mexicana 2-oxoglutarate + CO2 + NADPH + H+
-
 ?

Subunits

EC Number Subunits Comment Organism
1.1.1.41 ? x * 46200, calculated from amino acid sequence Leishmania mexicana
1.1.1.42 ? x * 48100, calculated from amino acid sequence Leishmania mexicana

Synonyms

EC Number Synonyms Comment Organism
1.1.1.41 IDH
-
 Leishmania mexicana
1.1.1.41 IDH-50
-
 Leishmania mexicana
1.1.1.42 IDH
-
 Leishmania mexicana
1.1.1.42 IDH-90
-
 Leishmania mexicana

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.1.1.41 78.98
-
 DL-isocitrate at pH 7.4 and 37°C Leishmania mexicana
1.1.1.41 84.39
-
 NAD+ at pH 7.4 and 37°C Leishmania mexicana
1.1.1.42 83.03
-
 DL-isocitrate at pH 7.4 and 37°C Leishmania mexicana
1.1.1.42 84.29
-
 NADP+ at pH 7.4 and 37°C Leishmania mexicana

Cofactor

EC Number Cofactor Comment Organism Structure
1.1.1.41 NAD+ the enzyme isoform IDH-50 exclusively catalyzes the reduction of NAD+ Leishmania mexicana
1.1.1.42 NADP+ the enzyme isoform IDH-90 exclusively catalyzes the reduction of NADP+ Leishmania mexicana

pI Value

EC Number Organism Comment pI Value Maximum pI Value
1.1.1.41 Leishmania mexicana calculated from amino acid sequence
-
 5.5
1.1.1.42 Leishmania mexicana calculated from amino acid sequence
-
 8

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
1.1.1.41 1690
-
 NAD+ at pH 7.4 and 37°C Leishmania mexicana
1.1.1.41 39500
-
 DL-isocitrate at pH 7.4 and 37°C Leishmania mexicana
1.1.1.42 1930
-
 DL-isocitrate at pH 7.4 and 37°C Leishmania mexicana
1.1.1.42 5620
-
 NADP+ at pH 7.4 and 37°C Leishmania mexicana