Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Reshetnikov, A.; Solntseva, N.; Rozova, O.; Mustakhimov, I.; Trotsenko, Y.; Khmelenina, V.
    ATP-and polyphosphate-dependent glucokinases from aerobic methanotrophs (2019), Microorganisms, 7, 52 .
    View publication on PubMedView publication on EuropePMC

Activating Compound

EC Number Activating Compound Comment Organism Structure
2.7.1.2 AMP 0.5 M, 1.1fold activation Methylomonas methanica
2.7.1.2 fructose-6-phosphate 5 mM, 1.16fold activation Methylomonas methanica
2.7.1.2 pyruvate 5 mM, 1.15fold activation Methylomonas methanica
2.7.1.63 Adenosine triphosphate 5 mM, 1.55fold activation Methylomonas methanica
2.7.1.63 glyceraldehyde-3-phosphate slight activation Methylomonas methanica
2.7.1.63 phosphoenolpyruvate 5 mM, 1.11fold activation Methylomonas methanica
2.7.1.63 ribose-5-phosphate 5 mM, 1.18fold activation Methylomonas methanica

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.7.1.2 heterologous expression in Escherichia coli Methylomonas methanica
2.7.1.63 heterologous expression in Esherichia coli Methylomonas methanica

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.7.1.2 ADP 0.5 M, 51% loss of activity Methylomonas methanica
2.7.1.2 citrate 1 M, 23% loss of activity Methylomonas methanica
2.7.1.2 KH2PO4 3 M, 14% loss of activity Methylomonas methanica
2.7.1.2 NaCl 0.5 M, 50% loss of activity Methylomonas methanica
2.7.1.2 Pyrophosphate 2 M, 18% loss of activity Methylomonas methanica
2.7.1.63 ADP 0.5 M, 7% loss of activity Methylomonas methanica
2.7.1.63 AMP 0.5 M, 16% loss of activity Methylomonas methanica

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.7.1.2 0.078
-
ATP 30°C, pH 7.5 Methylomonas methanica
2.7.1.2 0.08
-
D-glucose 30°C, pH 7.5 Methylomonas methanica
2.7.1.63 0.021
-
(phosphate)45 28°C, pH 8.5 Methylomonas methanica
2.7.1.63 0.45
-
D-glucose 28°C, pH 8.5 Methylomonas methanica

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.7.1.2 Cd2+ 11% of the activity compared to Mg2+ Methylomonas methanica
2.7.1.2 Co2+ 18% of the activity compared to Mg2+ Methylomonas methanica
2.7.1.2 Cs+ 52% of the activity compared to Mg2+ Methylomonas methanica
2.7.1.2 Cu2+ 3% of the activity compared to Mg2+ Methylomonas methanica
2.7.1.2 Li+ 72% of the activity compared to Mg2+ Methylomonas methanica
2.7.1.2 Mg2+ highest activity is observed in the presence of 5 mM of MgCl2 Methylomonas methanica
2.7.1.2 Mn2+ 60% of the activity compared to Mg2+ Methylomonas methanica
2.7.1.2 Na2MoO4 66% of the activity compared to Mg2+ Methylomonas methanica
2.7.1.2 Ni2+ 13% of the activity compared to Mg2+ Methylomonas methanica
2.7.1.2 Rb+ 73% of the activity compared to Mg2+ Methylomonas methanica
2.7.1.2 Sr2+ 30% of the activity compared to Mg2+ Methylomonas methanica

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.7.1.2 35500
-
SDS-PAGE Methylomonas methanica
2.7.1.2 71000
-
gradient electrophoresis, non-denaturation conditions, dimer Methylomonas methanica
2.7.1.2 106500
-
gradient electrophoresis, non-denaturation conditions, trimer Methylomonas methanica
2.7.1.2 142000
-
gradient electrophoresis, non-denaturation conditions, tetramer Methylomonas methanica
2.7.1.2 177500
-
gradient electrophoresis, non-denaturation conditions, pentamer Methylomonas methanica
2.7.1.2 213000
-
gradient electrophoresis, non-denaturation conditions, hexamer Methylomonas methanica
2.7.1.63 6000
-
gel filtration, dimerization in presence of polyP Methylomonas methanica
2.7.1.63 30000
-
SDS-PAGE Methylomonas methanica
2.7.1.63 30000
-
gel filtration in absence of polyP Methylomonas methanica

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.7.1.63 (phosphate)n + D-glucose Methylomonas methanica
-
(phosphate)n-1 + D-glucose 6-phosphate
-
?
2.7.1.63 (phosphate)n + D-glucose Methylomonas methanica 12
-
(phosphate)n-1 + D-glucose 6-phosphate
-
?

Organism

EC Number Organism UniProt Comment Textmining
2.7.1.2 Methylomonas methanica A0A3G4RI75
-
-
2.7.1.2 Methylomonas methanica 12 A0A3G4RI75
-
-
2.7.1.63 Methylomonas methanica A0A3G4RHV5
-
-
2.7.1.63 Methylomonas methanica 12 A0A3G4RHV5
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.7.1.2
-
Methylomonas methanica
2.7.1.63
-
Methylomonas methanica

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.1.2 ATP + D-glucosamine
-
Methylomonas methanica ADP + D-glucosamine 6-phosphate
-
?
2.7.1.2 ATP + D-glucosamine
-
Methylomonas methanica 12 ADP + D-glucosamine 6-phosphate
-
?
2.7.1.2 ATP + D-glucose no activity with CTP, ADP, PPi or polyP Methylomonas methanica ADP + D-glucose 6-phosphate
-
?
2.7.1.2 ATP + D-glucose no activity with CTP, ADP, PPi or polyP Methylomonas methanica 12 ADP + D-glucose 6-phosphate
-
?
2.7.1.2 GTP + D-glucose 71% compared to the activity with ATP Methylomonas methanica GDP + D-glucose 6-phosphate
-
?
2.7.1.2 GTP + D-glucose 71% compared to the activity with ATP Methylomonas methanica 12 GDP + D-glucose 6-phosphate
-
?
2.7.1.2 UTP + D-glucose 85% compared to the activity with ATP Methylomonas methanica UDP + D-glucose 6-phosphate
-
?
2.7.1.2 UTP + D-glucose 85% compared to the activity with ATP Methylomonas methanica 12 UDP + D-glucose 6-phosphate
-
?
2.7.1.63 (phosphate)45 + D-glucose
-
Methylomonas methanica (phosphate)44 + D-glucose 6-phosphate
-
?
2.7.1.63 (phosphate)45 + D-glucose
-
Methylomonas methanica 12 (phosphate)44 + D-glucose 6-phosphate
-
?
2.7.1.63 (phosphate)n + D-glucose
-
Methylomonas methanica (phosphate)n-1 + D-glucose 6-phosphate
-
?
2.7.1.63 (phosphate)n + D-glucose
-
Methylomonas methanica 12 (phosphate)n-1 + D-glucose 6-phosphate
-
?

Subunits

EC Number Subunits Comment Organism
2.7.1.2 ? x * 35500, SDS-PAGE, multimeric protein consisting of di-, tri-, tetra-, penta- and hexamers Methylomonas methanica
2.7.1.63 ? x * 30000, SDS-PAGE, monomer oder dimer, dimerization in in presence of polyP Methylomonas methanica

Synonyms

EC Number Synonyms Comment Organism
2.7.1.2 ATP-dependent glucokinase
-
Methylomonas methanica
2.7.1.2 ATP-GLK
-
Methylomonas methanica
2.7.1.2 glk
-
Methylomonas methanica
2.7.1.63 PolyP-dependent glucokinase
-
Methylomonas methanica
2.7.1.63 polyP-Glk
-
Methylomonas methanica
2.7.1.63 polyphosphate-dependent glucokinase
-
Methylomonas methanica
2.7.1.63 PPGK
-
Methylomonas methanica

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.7.1.2 70
-
-
Methylomonas methanica
2.7.1.63 65
-
-
Methylomonas methanica

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
2.7.1.2 65
-
5 min, complete inactivation Methylomonas methanica
2.7.1.63 28
-
2 h, 30% loss of activity Methylomonas methanica
2.7.1.63 35
-
2 h, 64% loss of activity Methylomonas methanica
2.7.1.63 40
-
2 h, 95% loss of activity Methylomonas methanica
2.7.1.63 55
-
30 min, complete inactivation Methylomonas methanica
2.7.1.63 60 65 5 min, complete inactivation Methylomonas methanica

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2.7.1.2 0.1095
-
D-glucose 30°C, pH 7.5 Methylomonas methanica
2.7.1.2 0.126
-
ATP 30°C, pH 7.5 Methylomonas methanica
2.7.1.63 0.0066
-
D-glucose 28°C, pH 8.5 Methylomonas methanica
2.7.1.63 0.0068
-
(phosphate)45 28°C, pH 8.5 Methylomonas methanica

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.7.1.2 7.5
-
-
Methylomonas methanica
2.7.1.63 8.5
-
-
Methylomonas methanica

pH Range

EC Number pH Minimum pH Maximum Comment Organism
2.7.1.2 5 9.5 active in a wide range pH 5 to 9.5, sharp loss of activity above pH 9.5 Methylomonas methanica
2.7.1.63 5.5 10.5 active at a pH range of 5.5-10.5 Methylomonas methanica

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
2.7.1.2 1.37
-
D-glucose 30°C, pH 7.5 Methylomonas methanica
2.7.1.2 1.61
-
ATP 30°C, pH 7.5 Methylomonas methanica
2.7.1.63 0.0145
-
D-glucose 28°C, pH 8.5 Methylomonas methanica
2.7.1.63 0.33
-
(phosphate)45 28°C, pH 8.5 Methylomonas methanica