EC Number | Activating Compound | Comment | Organism | Structure |
---|---|---|---|---|
2.7.1.2 | AMP | 0.5 M, 1.1fold activation | Methylomonas methanica | |
2.7.1.2 | fructose-6-phosphate | 5 mM, 1.16fold activation | Methylomonas methanica | |
2.7.1.2 | pyruvate | 5 mM, 1.15fold activation | Methylomonas methanica | |
2.7.1.63 | Adenosine triphosphate | 5 mM, 1.55fold activation | Methylomonas methanica | |
2.7.1.63 | glyceraldehyde-3-phosphate | slight activation | Methylomonas methanica | |
2.7.1.63 | phosphoenolpyruvate | 5 mM, 1.11fold activation | Methylomonas methanica | |
2.7.1.63 | ribose-5-phosphate | 5 mM, 1.18fold activation | Methylomonas methanica |
EC Number | Cloned (Comment) | Organism |
---|---|---|
2.7.1.2 | heterologous expression in Escherichia coli | Methylomonas methanica |
2.7.1.63 | heterologous expression in Esherichia coli | Methylomonas methanica |
EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
2.7.1.2 | ADP | 0.5 M, 51% loss of activity | Methylomonas methanica | |
2.7.1.2 | citrate | 1 M, 23% loss of activity | Methylomonas methanica | |
2.7.1.2 | KH2PO4 | 3 M, 14% loss of activity | Methylomonas methanica | |
2.7.1.2 | NaCl | 0.5 M, 50% loss of activity | Methylomonas methanica | |
2.7.1.2 | Pyrophosphate | 2 M, 18% loss of activity | Methylomonas methanica | |
2.7.1.63 | ADP | 0.5 M, 7% loss of activity | Methylomonas methanica | |
2.7.1.63 | AMP | 0.5 M, 16% loss of activity | Methylomonas methanica |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
2.7.1.2 | 0.078 | - |
ATP | 30°C, pH 7.5 | Methylomonas methanica | |
2.7.1.2 | 0.08 | - |
D-glucose | 30°C, pH 7.5 | Methylomonas methanica | |
2.7.1.63 | 0.021 | - |
(phosphate)45 | 28°C, pH 8.5 | Methylomonas methanica | |
2.7.1.63 | 0.45 | - |
D-glucose | 28°C, pH 8.5 | Methylomonas methanica |
EC Number | Metals/Ions | Comment | Organism | Structure |
---|---|---|---|---|
2.7.1.2 | Cd2+ | 11% of the activity compared to Mg2+ | Methylomonas methanica | |
2.7.1.2 | Co2+ | 18% of the activity compared to Mg2+ | Methylomonas methanica | |
2.7.1.2 | Cs+ | 52% of the activity compared to Mg2+ | Methylomonas methanica | |
2.7.1.2 | Cu2+ | 3% of the activity compared to Mg2+ | Methylomonas methanica | |
2.7.1.2 | Li+ | 72% of the activity compared to Mg2+ | Methylomonas methanica | |
2.7.1.2 | Mg2+ | highest activity is observed in the presence of 5 mM of MgCl2 | Methylomonas methanica | |
2.7.1.2 | Mn2+ | 60% of the activity compared to Mg2+ | Methylomonas methanica | |
2.7.1.2 | Na2MoO4 | 66% of the activity compared to Mg2+ | Methylomonas methanica | |
2.7.1.2 | Ni2+ | 13% of the activity compared to Mg2+ | Methylomonas methanica | |
2.7.1.2 | Rb+ | 73% of the activity compared to Mg2+ | Methylomonas methanica | |
2.7.1.2 | Sr2+ | 30% of the activity compared to Mg2+ | Methylomonas methanica |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
2.7.1.2 | 35500 | - |
SDS-PAGE | Methylomonas methanica |
2.7.1.2 | 71000 | - |
gradient electrophoresis, non-denaturation conditions, dimer | Methylomonas methanica |
2.7.1.2 | 106500 | - |
gradient electrophoresis, non-denaturation conditions, trimer | Methylomonas methanica |
2.7.1.2 | 142000 | - |
gradient electrophoresis, non-denaturation conditions, tetramer | Methylomonas methanica |
2.7.1.2 | 177500 | - |
gradient electrophoresis, non-denaturation conditions, pentamer | Methylomonas methanica |
2.7.1.2 | 213000 | - |
gradient electrophoresis, non-denaturation conditions, hexamer | Methylomonas methanica |
2.7.1.63 | 6000 | - |
gel filtration, dimerization in presence of polyP | Methylomonas methanica |
2.7.1.63 | 30000 | - |
SDS-PAGE | Methylomonas methanica |
2.7.1.63 | 30000 | - |
gel filtration in absence of polyP | Methylomonas methanica |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
2.7.1.63 | (phosphate)n + D-glucose | Methylomonas methanica | - |
(phosphate)n-1 + D-glucose 6-phosphate | - |
? | |
2.7.1.63 | (phosphate)n + D-glucose | Methylomonas methanica 12 | - |
(phosphate)n-1 + D-glucose 6-phosphate | - |
? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
2.7.1.2 | Methylomonas methanica | A0A3G4RI75 | - |
- |
2.7.1.2 | Methylomonas methanica 12 | A0A3G4RI75 | - |
- |
2.7.1.63 | Methylomonas methanica | A0A3G4RHV5 | - |
- |
2.7.1.63 | Methylomonas methanica 12 | A0A3G4RHV5 | - |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
2.7.1.2 | - |
Methylomonas methanica |
2.7.1.63 | - |
Methylomonas methanica |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
2.7.1.2 | ATP + D-glucosamine | - |
Methylomonas methanica | ADP + D-glucosamine 6-phosphate | - |
? | |
2.7.1.2 | ATP + D-glucosamine | - |
Methylomonas methanica 12 | ADP + D-glucosamine 6-phosphate | - |
? | |
2.7.1.2 | ATP + D-glucose | no activity with CTP, ADP, PPi or polyP | Methylomonas methanica | ADP + D-glucose 6-phosphate | - |
? | |
2.7.1.2 | ATP + D-glucose | no activity with CTP, ADP, PPi or polyP | Methylomonas methanica 12 | ADP + D-glucose 6-phosphate | - |
? | |
2.7.1.2 | GTP + D-glucose | 71% compared to the activity with ATP | Methylomonas methanica | GDP + D-glucose 6-phosphate | - |
? | |
2.7.1.2 | GTP + D-glucose | 71% compared to the activity with ATP | Methylomonas methanica 12 | GDP + D-glucose 6-phosphate | - |
? | |
2.7.1.2 | UTP + D-glucose | 85% compared to the activity with ATP | Methylomonas methanica | UDP + D-glucose 6-phosphate | - |
? | |
2.7.1.2 | UTP + D-glucose | 85% compared to the activity with ATP | Methylomonas methanica 12 | UDP + D-glucose 6-phosphate | - |
? | |
2.7.1.63 | (phosphate)45 + D-glucose | - |
Methylomonas methanica | (phosphate)44 + D-glucose 6-phosphate | - |
? | |
2.7.1.63 | (phosphate)45 + D-glucose | - |
Methylomonas methanica 12 | (phosphate)44 + D-glucose 6-phosphate | - |
? | |
2.7.1.63 | (phosphate)n + D-glucose | - |
Methylomonas methanica | (phosphate)n-1 + D-glucose 6-phosphate | - |
? | |
2.7.1.63 | (phosphate)n + D-glucose | - |
Methylomonas methanica 12 | (phosphate)n-1 + D-glucose 6-phosphate | - |
? |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
2.7.1.2 | ? | x * 35500, SDS-PAGE, multimeric protein consisting of di-, tri-, tetra-, penta- and hexamers | Methylomonas methanica |
2.7.1.63 | ? | x * 30000, SDS-PAGE, monomer oder dimer, dimerization in in presence of polyP | Methylomonas methanica |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
2.7.1.2 | ATP-dependent glucokinase | - |
Methylomonas methanica |
2.7.1.2 | ATP-GLK | - |
Methylomonas methanica |
2.7.1.2 | glk | - |
Methylomonas methanica |
2.7.1.63 | PolyP-dependent glucokinase | - |
Methylomonas methanica |
2.7.1.63 | polyP-Glk | - |
Methylomonas methanica |
2.7.1.63 | polyphosphate-dependent glucokinase | - |
Methylomonas methanica |
2.7.1.63 | PPGK | - |
Methylomonas methanica |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
2.7.1.2 | 70 | - |
- |
Methylomonas methanica |
2.7.1.63 | 65 | - |
- |
Methylomonas methanica |
EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|---|
2.7.1.2 | 65 | - |
5 min, complete inactivation | Methylomonas methanica |
2.7.1.63 | 28 | - |
2 h, 30% loss of activity | Methylomonas methanica |
2.7.1.63 | 35 | - |
2 h, 64% loss of activity | Methylomonas methanica |
2.7.1.63 | 40 | - |
2 h, 95% loss of activity | Methylomonas methanica |
2.7.1.63 | 55 | - |
30 min, complete inactivation | Methylomonas methanica |
2.7.1.63 | 60 | 65 | 5 min, complete inactivation | Methylomonas methanica |
EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
2.7.1.2 | 0.1095 | - |
D-glucose | 30°C, pH 7.5 | Methylomonas methanica | |
2.7.1.2 | 0.126 | - |
ATP | 30°C, pH 7.5 | Methylomonas methanica | |
2.7.1.63 | 0.0066 | - |
D-glucose | 28°C, pH 8.5 | Methylomonas methanica | |
2.7.1.63 | 0.0068 | - |
(phosphate)45 | 28°C, pH 8.5 | Methylomonas methanica |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
2.7.1.2 | 7.5 | - |
- |
Methylomonas methanica |
2.7.1.63 | 8.5 | - |
- |
Methylomonas methanica |
EC Number | pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|---|
2.7.1.2 | 5 | 9.5 | active in a wide range pH 5 to 9.5, sharp loss of activity above pH 9.5 | Methylomonas methanica |
2.7.1.63 | 5.5 | 10.5 | active at a pH range of 5.5-10.5 | Methylomonas methanica |
EC Number | kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
2.7.1.2 | 1.37 | - |
D-glucose | 30°C, pH 7.5 | Methylomonas methanica | |
2.7.1.2 | 1.61 | - |
ATP | 30°C, pH 7.5 | Methylomonas methanica | |
2.7.1.63 | 0.0145 | - |
D-glucose | 28°C, pH 8.5 | Methylomonas methanica | |
2.7.1.63 | 0.33 | - |
(phosphate)45 | 28°C, pH 8.5 | Methylomonas methanica |