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Literature summary extracted from

  • Wu, B.; Yu, Q.; Zheng, S.; Pedroso, M.; Guddat, L.; He, B.; Schenk, G.
    Relative catalytic efficiencies and transcript levels of three d- and two l-lactate dehydrogenases for optically pure d-lactate production in Sporolactobacillus inulinus (2019), MicrobiologyOpen, 8, e704 .
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.1.1.27 expression in Escherichia coli Sporolactobacillus inulinus
1.1.1.28 expression in Escherichia coli Sporolactobacillus inulinus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.1.1.27 4.1
-
 pyruvate isoform LDHL1, pH 7, 45°C Sporolactobacillus inulinus
1.1.1.27 6.8
-
 pyruvate isoform LDHL2, pH 7, 40°C Sporolactobacillus inulinus
1.1.1.28 0.47
-
 pyruvate isoform LDHD1, pH 6, 35°C Sporolactobacillus inulinus
1.1.1.28 1.4
-
 pyruvate isoform LDHD2, pH 7.5, 35°C Sporolactobacillus inulinus
1.1.1.28 3.1
-
 pyruvate isoform LDHD3, pH 5.5, 35°C Sporolactobacillus inulinus

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.1.1.27 Fe3+ 5 mM, about 10% residual activity Sporolactobacillus inulinus
1.1.1.28 Fe3+ 5 mM, about 10% residual activity Sporolactobacillus inulinus

Organism

EC Number Organism UniProt Comment Textmining
1.1.1.27 Sporolactobacillus inulinus
-
-
-
1.1.1.27 Sporolactobacillus inulinus YBS1-5
-
-
-
1.1.1.28 Sporolactobacillus inulinus
-
-
-
1.1.1.28 Sporolactobacillus inulinus YBS1-5
-
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.1.27 2-oxobutanoate + NADH + H+
-
 Sporolactobacillus inulinus 2-hydroxybutyrate + NAD+
-
 ?
1.1.1.27 2-oxobutanoate + NADH + H+
-
 Sporolactobacillus inulinus YBS1-5 2-hydroxybutyrate + NAD+
-
 ?
1.1.1.27 2-oxovalerate + NADH + H+
-
 Sporolactobacillus inulinus 2-hydroxyvalerate + NAD+
-
 ?
1.1.1.27 2-oxovalerate + NADH + H+
-
 Sporolactobacillus inulinus YBS1-5 2-hydroxyvalerate + NAD+
-
 ?
1.1.1.27 glyoxylate + NADH + H+ best substrate of isoform LDHL1 Sporolactobacillus inulinus glycolate + NAD+
-
 ?
1.1.1.27 glyoxylate + NADH + H+ best substrate of isoform LDHL1 Sporolactobacillus inulinus YBS1-5 glycolate + NAD+
-
 ?
1.1.1.27 phenylpyruvate + NADH + H+
-
 Sporolactobacillus inulinus 2-hydroxy-3-phenylpropanoate + NAD+
-
 ?
1.1.1.27 phenylpyruvate + NADH + H+
-
 Sporolactobacillus inulinus YBS1-5 2-hydroxy-3-phenylpropanoate + NAD+
-
 ?
1.1.1.27 pyruvate + NADH + H+
-
 Sporolactobacillus inulinus L-lactate + NAD+
-
 ?
1.1.1.27 pyruvate + NADH + H+
-
 Sporolactobacillus inulinus YBS1-5 L-lactate + NAD+
-
 ?
1.1.1.28 2-oxobutanoate + NADH + H+
-
 Sporolactobacillus inulinus 2-hydroxybutanoate + NAD+
-
 ?
1.1.1.28 2-oxovalerate + NADH + H+
-
 Sporolactobacillus inulinus 2-hydroxyvalerate + NAD+
-
 ?
1.1.1.28 glyoxylate + NADH + H+ best substrate of isoform LDHL1 Sporolactobacillus inulinus glycolate + NAD+
-
 ?
1.1.1.28 phenylpyruvate + NADH + H+
-
 Sporolactobacillus inulinus 2-hydroxy-3-phenylpropanoate + NAD+
-
 ?
1.1.1.28 pyruvate + NADH + H+
-
 Sporolactobacillus inulinus D-lactate + NAD+
-
 ?

Subunits

EC Number Subunits Comment Organism
1.1.1.27 ? x * 34400, isoform LDHL2, calculated from sequence and SDS-PAGE Sporolactobacillus inulinus
1.1.1.27 ? x * 35500, isoform LDHL1, calculated from sequence and SDS-PAGE Sporolactobacillus inulinus
1.1.1.28 ? x * 36600, isoform LDHD2, calculated from sequence and SDS-PAGE Sporolactobacillus inulinus
1.1.1.28 ? x * 36700, isoform LDHD1, calculated from sequence and SDS-PAGE Sporolactobacillus inulinus
1.1.1.28 ? x * 37200, isoform LDHD2, calculated from sequence and SDS-PAGE Sporolactobacillus inulinus

Synonyms

EC Number Synonyms Comment Organism
1.1.1.27 LDHL1
-
 Sporolactobacillus inulinus
1.1.1.27 LDHL2
-
 Sporolactobacillus inulinus
1.1.1.28 LDHD1
-
 Sporolactobacillus inulinus
1.1.1.28 LDHD2
-
 Sporolactobacillus inulinus
1.1.1.28 LDHD3
-
 Sporolactobacillus inulinus

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.1.1.27 2.3
-
 pyruvate isoform LDHL2, pH 7, 40°C Sporolactobacillus inulinus
1.1.1.27 5.9
-
 pyruvate isoform LDHL1, pH 7, 45°C Sporolactobacillus inulinus
1.1.1.28 10.7
-
 pyruvate isoform LDHD3, pH 5.5, 35°C Sporolactobacillus inulinus
1.1.1.28 44.7
-
 pyruvate isoform LDHD2, pH 7.5, 35°C Sporolactobacillus inulinus
1.1.1.28 75.8
-
 pyruvate isoform LDHD1, pH 6, 35°C Sporolactobacillus inulinus

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
1.1.1.27 0.3
-
 pyruvate isoform LDHL2, pH 7, 40°C Sporolactobacillus inulinus
1.1.1.27 1.4
-
 pyruvate isoform LDHL1, pH 7, 45°C Sporolactobacillus inulinus
1.1.1.28 3.5
-
 pyruvate isoform LDHD3, pH 5.5, 35°C Sporolactobacillus inulinus
1.1.1.28 32
-
 pyruvate isoform LDHD2, pH 7.5, 35°C Sporolactobacillus inulinus
1.1.1.28 161
-
 pyruvate isoform LDHD1, pH 6, 35°C Sporolactobacillus inulinus