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Literature summary extracted from

  • Bali, A.P.; Lennox-Hvenekilde, D.; Myling-Petersen, N.; Buerger, J.; Salomonsen, B.; Gronenberg, L.S.; Sommer, M.O.A.; Genee, H.J.
    Improved biotin, thiamine, and lipoic acid biosynthesis by engineering the global regulator IscR (2020), Metab. Eng., 60, 97-109 .
    View publication on PubMed

Organism

EC Number Organism UniProt Comment Textmining
2.8.1.6 Escherichia coli
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2.8.1.6 Escherichia coli BW25113
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General Information

EC Number General Information Comment Organism
2.8.1.6 malfunction single mutations in the global regulator IscR can substantially improve cellular tolerance to BioB overexpression, increasing Escherichia coli dethiobiotin-to-biotin biocatalysis by more than 2.2fold. In the overexpression strains, FeS-cluster depletion is the main reason for toxicity. IscR mutations significantly affect cell viability and improve cell factories for de novo biosynthesis of thiamine by 1.3fold and lipoic acid by 1.8fold Escherichia coli