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Literature summary extracted from

  • Deka, R.; Liu, W.; Norgard, M.; Brautigam, C.
    Biophysical and biochemical characterization of tp0037, a D-lactate dehydrogenase, supports an acetogenic energy conservation pathway in Treponema pallidum (2020), mBio, 11, e02249 .
    View publication on PubMedView publication on EuropePMC

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
1.1.1.28 to 1.38 A resolution. The structure features two domains with a cleft between them. The smaller catalytic domain comprises a central beta-sheet flanked by alpha-helices. The sheet is composed of five parallel strands and one antiparallel one. The larger nucleotide-binding domain harbors a centrally located seven-strand parallel beta-sheet Treponema pallidum

Protein Variants

EC Number Protein Variants Comment Organism
1.1.1.28 H296A loss of activity Treponema pallidum
1.1.1.28 R235A loss of activity Treponema pallidum
1.1.1.28 Y101A strong decrease in activity Treponema pallidum

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.1.1.28 78100
-
 dynamic light scattering Treponema pallidum

Organism

EC Number Organism UniProt Comment Textmining
1.1.1.28 Treponema pallidum O83080
-
-
1.1.1.28 Treponema pallidum Nichols O83080
-
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.1.1.28 0.4
-
 mutant Y101A, substrate L-lactate, 37°C, pH not specified in the publication Treponema pallidum
1.1.1.28 7
-
 wild-type, substrate L-lactate, 37°C, pH not specified in the publication Treponema pallidum
1.1.1.28 172
-
 mutant Y101A, substrate D-lactate, 37°C, pH not specified in the publication Treponema pallidum
1.1.1.28 5000
-
 wild-type, substrate D-lactate, 37°C, pH not specified in the publication Treponema pallidum

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.1.28 D-lactate + NAD+
-
 Treponema pallidum pyruvate + NADH + H+
-
 ?
1.1.1.28 D-lactate + NAD+
-
 Treponema pallidum Nichols pyruvate + NADH + H+
-
 ?
1.1.1.28 L-lactate + NAD+ very poor substrate Treponema pallidum pyruvate + NADH + H+
-
 ?
1.1.1.28 L-lactate + NAD+ very poor substrate Treponema pallidum Nichols pyruvate + NADH + H+
-
 ?

Subunits

EC Number Subunits Comment Organism
1.1.1.28 dimer 2 * 39190, calculated from sequence Treponema pallidum

Synonyms

EC Number Synonyms Comment Organism
1.1.1.28 tp0037
-
 Treponema pallidum

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
1.1.1.28 48.7
-
 mutant H296A, melting temperature Treponema pallidum
1.1.1.28 49.1
-
 wild-type, melting temperature Treponema pallidum
1.1.1.28 49.3
-
 wild-type, melting temperature, presence of 20 mM L-lactate Treponema pallidum
1.1.1.28 50.1
-
 wild-type, melting temperature, presence of 20 mM D-lactate Treponema pallidum
1.1.1.28 53.4
-
 wild-type, melting temperature, presence of 5 mM NAD+ Treponema pallidum
1.1.1.28 53.5
-
 mutant Y101A, melting temperature Treponema pallidum
1.1.1.28 55.8
-
 mutant R235A, melting temperature Treponema pallidum