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Literature summary extracted from

  • Zhu, L.; Liu, R.; Liu, T.; Zou, X.; Xu, Z.; Guan, H.
    A novel strategy to screen inhibitors of multiple aminoglycoside-modifying enzymes with ultra-high performance liquid chromatography-quadrupole-time-of-flight mass spectrometry (2019), J. Pharm. Biomed. Anal., 164, 520-527 .
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.7.1.190 additional information inhibitor screening of a chemical library using ultra-high performance liquid chromatography-quadrupole-time-of-flight mass spectrometry, assay method optimization and validation, overview. Poor inhibition by Mer-C-3, Mer-C-8e, and paromomycin Staphylococcus aureus
2.7.1.190 quercetin
-
 Staphylococcus aureus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.7.1.190 additional information
-
 additional information Michaelis-Menten kinetics Staphylococcus aureus
2.7.1.190 0.00441
-
 kanamycin pH 7.5, 37°C Staphylococcus aureus

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.7.1.190 Mg2+ required Staphylococcus aureus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.7.1.190 GTP + kanamycin Staphylococcus aureus
-
 GDP + kanamycin 2''-phosphate
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.7.1.190 Staphylococcus aureus P0A0C1
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.1.190 GTP + kanamycin
-
 Staphylococcus aureus GDP + kanamycin 2''-phosphate
-
 ?
2.7.1.190 additional information the bifunctional enzyme AAC(6')-APH(2'') enzyme shows acetyltransferase activity and phosphotransferase activity, activity measurement in a sigle assay. GTP is more qualified as a phosphate donor than ATP to cause phosphorylation events of AAC(6')-APH(2'') Staphylococcus aureus ?
-
-

Synonyms

EC Number Synonyms Comment Organism
2.7.1.190 AAC(6')-APH(2'')
-
 Staphylococcus aureus
2.7.1.190 AME
-
 Staphylococcus aureus
2.7.1.190 aminoglycoside-modification enzyme
-
 Staphylococcus aureus
2.7.1.190 APH(2'')
-
 Staphylococcus aureus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.7.1.190 37
-
 assay at Staphylococcus aureus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.7.1.190 7.5
-
 assay at Staphylococcus aureus

Cofactor

EC Number Cofactor Comment Organism Structure
2.7.1.190 GTP
-
 Staphylococcus aureus
2.7.1.190 additional information GTP is more qualified as a phosphate donor than ATP to cause phosphorylation events of AAC(6')-APH(2'') Staphylococcus aureus

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
2.7.1.190 0.01761
-
 quercetin pH 7.5, 37°C Staphylococcus aureus

IC50 Value

EC Number IC50 Value IC50 Value Maximum Comment Organism Inhibitor Structure
2.7.1.190 0.03074
-
 pH 7.5, 37°C Staphylococcus aureus quercetin