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Literature summary extracted from

  • Sossna-Wunder, G.; Hengstenberg, W.; Briozzo, P.; Deutscher, J.
    Photoinactivation of the Staphylococcus aureus lactose-specific EIICB phosphotransferase component with p-azidophenyl-beta-D-galactoside and phosphorylation of the covalently bound substrate (2018), J. Mol. Microbiol. Biotechnol., 28, 147-158 .
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.7.1.207
-
 Staphylococcus aureus

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.7.1.207 4-azidophenyl-beta-D-galactoside irradiation of a reaction mixture containing 0.25 mM of photoactivatable 4-azidophenyl-beta-D-galactopyranoside and enzyme with UV light at 254 nm for 3 min causes approximately 90% loss of enzyme activity. When the photoinactivation experiments are carried out in the presence of 1 mM lactose, the loss of enzyme activity is significantly slowed Staphylococcus aureus

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.7.1.207 membrane
-
 Staphylococcus aureus 16020
-

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.7.1.207 [protein]-Npi-phospho-L-histidine + lactose[side 1] Staphylococcus aureus
-
 [protein]-L-histidine + lactose 6'-phosphate[side 2]
-
 ?
2.7.1.207 [protein]-Npi-phospho-L-histidine + lactose[side 1] Staphylococcus aureus S305A
-
 [protein]-L-histidine + lactose 6'-phosphate[side 2]
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.7.1.207 Staphylococcus aureus
-
-
-
2.7.1.207 Staphylococcus aureus S305A
-
-
-

Posttranslational Modification

EC Number Posttranslational Modification Comment Organism
2.7.1.207 phosphoprotein the enzyme's EIIB domain is phosphorylated at Cys476 Staphylococcus aureus

Purification (Commentary)

EC Number Purification (Comment) Organism
2.7.1.207
-
 Staphylococcus aureus

Storage Stability

EC Number Storage Stability Organism
2.7.1.207 -80°C, purified enzyme, several months, no significant loss of activity Staphylococcus aureus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.1.207 additional information 4-nitrophenyl-alpha-D-galactopyranoside is a poor substrate for the enzyme Staphylococcus aureus ?
-
-
2.7.1.207 additional information 4-nitrophenyl-alpha-D-galactopyranoside is a poor substrate for the enzyme Staphylococcus aureus S305A ?
-
-
2.7.1.207 [protein]-Npi-phospho-L-histidine + 4-nitrophenyl beta-D-galactoside[side 1]
-
 Staphylococcus aureus [protein]-L-histidine + 4-nitrophenyl beta-D-galactoside 6'-phosphate[side 2]
-
 ?
2.7.1.207 [protein]-Npi-phospho-L-histidine + 4-nitrophenyl beta-D-galactoside[side 1]
-
 Staphylococcus aureus S305A [protein]-L-histidine + 4-nitrophenyl beta-D-galactoside 6'-phosphate[side 2]
-
 ?
2.7.1.207 [protein]-Npi-phospho-L-histidine + lactose[side 1]
-
 Staphylococcus aureus [protein]-L-histidine + lactose 6'-phosphate[side 2]
-
 ?
2.7.1.207 [protein]-Npi-phospho-L-histidine + lactose[side 1]
-
 Staphylococcus aureus S305A [protein]-L-histidine + lactose 6'-phosphate[side 2]
-
 ?

Subunits

EC Number Subunits Comment Organism
2.7.1.207 ? x * 45000, SDS-PAGE Staphylococcus aureus
2.7.1.207 ? x * 62700, calculated from amino acid sequence Staphylococcus aureus

Synonyms

EC Number Synonyms Comment Organism
2.7.1.207 EIICBLac
-
 Staphylococcus aureus
2.7.1.207 lactose-specific permease enzyme IICB
-
 Staphylococcus aureus