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Literature summary extracted from

  • Newcomb, B.; Rhein, C.; Mileva, I.; Ahmad, R.; Clarke, C.J.; Snider, J.; Obeid, L.M.; Hannun, Y.A.
    Identification of an acid sphingomyelinase ceramide kinase pathway in the regulation of the chemokine CCL5 (2018), J. Lipid Res., 59, 1219-1229 .
    View publication on PubMedView publication on EuropePMC

Protein Variants

EC Number Protein Variants Comment Organism
2.7.1.138 additional information knockdown of CERK in MCF-7 cells by specific siRNA Homo sapiens

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.7.1.138 Mg2+ required Homo sapiens

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.7.1.138 ATP + ceramide Homo sapiens
-
 ADP + ceramide 1-phosphate
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.7.1.138 Homo sapiens Q8TCT0
-
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.7.1.138 breast cancer cell
-
 Homo sapiens
-
2.7.1.138 MCF-7 cell
-
 Homo sapiens
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.1.138 ATP + ceramide
-
 Homo sapiens ADP + ceramide 1-phosphate
-
 ?

Synonyms

EC Number Synonyms Comment Organism
2.7.1.138 CERK
-
 Homo sapiens

Cofactor

EC Number Cofactor Comment Organism Structure
2.7.1.138 ATP
-
 Homo sapiens

General Information

EC Number General Information Comment Organism
2.7.1.138 malfunction cells lacking acid sphingomyelinase (ASM) have decreased ceramide 1-phosphate production following TNF-alpha treatment, suggesting that ASM may be acting upstream of CERK. Effect of CERK knockdown on lipid levels of ceramide 1-phosphate, ceramide, and sphingosine, overview. Knockdown of CERK in the presence of ASM overexpression led to a decrease in CCL5 levels on the protein and message levels Homo sapiens
2.7.1.138 metabolism functional role of the ASM/CERK pathway in cancer cell migration, and of the ASM/CERK signaling axis in cytokines associated with EMT, cell migration, and invasiveness Homo sapiens
2.7.1.138 physiological function TNF-alpha induces the formation of ceramide 1-phosphate (C-1-P) in a CERK-dependent manner. Silencing of CERK blocks CCL5 production in response to TNF-alpha. ASM and CERK induce a highly concordant program of cytokine production and both are required for migration of breast cancer cells. ASM can produce ceramide which is then converted to ceramide 1-phosphate by CERK, and that ceramide 1-phosphate is required for production of CCL5 and several cytokines and chemokines, with roles in cell migration. Enzyme CERK is required for CCL5 production and sufficient to induce CCL5 in MCF-7 breast cancer cells Homo sapiens