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Literature summary extracted from

  • Feng, L.; Yuen, Y.L.; Xu, J.; Liu, X.; Chan, M.Y.; Wang, K.; Fong, W.P.; Cheung, W.T.; Lee, S.S.
    Identification and characterization of a novel PPARalpha-regulated and 7alpha-hydroxyl bile acid-preferring cytosolic sulfotransferase mL-STL (Sult2a8) (2017), J. Lipid Res., 58, 1114-1131 .
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.8.2.2 gene SULT2B1b, quantitative RT-PCR enzyme expression analysis, recombinant overexpression in LNCaP and DU-145 prostate cells Homo sapiens
2.8.2.14 gene Sult2a8, DNA and amino acid sequence determination and analysis, mL-STL cDNAs exhibit multiple 5' termini and skipping of nucleotides, sequence analysis of 12 mL-STL 3'-RACE clones. The mL-STL gene has multiple alternative exonic splicing variants, long and short forms of mL-STL cDNAs. The long-form mL-STL gene is composed of seven exons separated by six introns, with a total gene size of 35902 bp. Recombinant expression of non-tagged and His6-tagged enzyme in Escherichia coli strain BL21(DE3)pLysS, half of the expressed protein is soluble, whereas the remaining protein is misfolded to form inclusion bodies Mus musculus

Protein Variants

EC Number Protein Variants Comment Organism
2.8.2.2 additional information construction of SULT2B1b knockdown prostate cells using siRNA or shRNA targeting and LNCaP cells. SULT2B1b knockdown results in a decrease in growth/viability of LNCaP, VCaP, C4-2, and RWPE-1 cells as well as decreased softagar colony formation in LNCaP cells, and it induces cell death in prostate cancer cells. A significant decrease in cell growth/viability is observed using multiple SULT2B1b RNAi sequences in LNCaP cells. SULT2B1b knockdown increases the percentage of sub-G1 nuclei by cell-cycle analysis and significantly increased caspase-3 activity and PARP cleavage in LNCaP, VCaP, and C4-2 cells. LNCaP cells with SULT2B1b knockdown analyzed by flow cytometry show an increased percentage of Annexin V+/propidium iodide (PI)x02cells compared with control cells. Double KD of SULT2B1b/LXRbeta does not affect the siRNA knockdown efficiency compared with SULT2B1b or LXRbeta knockdown alone. LNCaP cells with SULT2B1b overexpression (hSULT2B1b vector) show a significant decrease in liver X receptor (LXR) activity and a trend of decreased transcription of downstream target gene, ATP-binding cassette (ABC)-G1. LXR activity in LNCaP cells has been demonstrated to be due to transcriptional activation of LXRbeta. Phenotype of SULT2B1b knockout cells, overview Homo sapiens

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.8.2.14 additional information no significant substrate inhibition is detected in most compounds tested as the substrate concentrations increased from 0.01 to 0.1 mM Mus musculus

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.8.2.2 cytosol
-
 Homo sapiens 5829
-
2.8.2.14 cytosol
-
 Mus musculus 5829
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.8.2.14 Mg2+ required Mus musculus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.8.2.2 3'-phosphoadenylyl sulfate + cholesterol Homo sapiens
-
 adenosine 3',5'-bisphosphate + cholesterol 3-sulfate
-
 ?
2.8.2.14 3'-phosphoadenylyl sulfate + alpha-muricholic acid Mus musculus
-
 adenosine 3',5'-bisphosphate + alpha-muricholate 3-sulfate
-
 ?
2.8.2.14 3'-phosphoadenylyl sulfate + alpha-muricholic acid Mus musculus SV/129
-
 adenosine 3',5'-bisphosphate + alpha-muricholate 3-sulfate
-
 ?
2.8.2.14 3'-phosphoadenylyl sulfate + beta-muricholic acid Mus musculus low activity adenosine 3',5'-bisphosphate + beta-muricholate 3-sulfate
-
 ?
2.8.2.14 3'-phosphoadenylyl sulfate + beta-muricholic acid Mus musculus SV/129 low activity adenosine 3',5'-bisphosphate + beta-muricholate 3-sulfate
-
 ?
2.8.2.14 3'-phosphoadenylyl sulfate + chenodeoxycholic acid Mus musculus
-
 adenosine 3',5'-bisphosphate + chenodeoxycholate 3-sulfate
-
 ?
2.8.2.14 3'-phosphoadenylyl sulfate + chenodeoxycholic acid Mus musculus SV/129
-
 adenosine 3',5'-bisphosphate + chenodeoxycholate 3-sulfate
-
 ?
2.8.2.14 3'-phosphoadenylyl sulfate + cholic acid Mus musculus
-
 adenosine 3',5'-bisphosphate + cholate 3-sulfate
-
 ?
2.8.2.14 3'-phosphoadenylyl sulfate + cholic acid Mus musculus SV/129
-
 adenosine 3',5'-bisphosphate + cholate 3-sulfate
-
 ?
2.8.2.14 3'-phosphoadenylyl sulfate + glycochenodeoxycholic acid Mus musculus
-
 adenosine 3',5'-bisphosphate + glycochenodeoxycholate 3-sulfate
-
 ?
2.8.2.14 3'-phosphoadenylyl sulfate + glycocholic acid Mus musculus
-
 adenosine 3',5'-bisphosphate + glycocholate 3-sulfate
-
 ?
2.8.2.14 3'-phosphoadenylyl sulfate + taurochenodeoxycholic acid Mus musculus
-
 adenosine 3',5'-bisphosphate + taurochenodeoxycholate 3-sulfate
-
 ?
2.8.2.14 3'-phosphoadenylyl sulfate + taurocholic acid Mus musculus
-
 adenosine 3',5'-bisphosphate + taurocholate 3-sulfate
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.8.2.2 Homo sapiens O00204
-
-
2.8.2.14 Mus musculus Q8BGL3
-
-
2.8.2.14 Mus musculus SV/129 Q8BGL3
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.8.2.14 recombinant His6-tagged enzyme from Escherichia coli strain BL21(DE3)pLysS by nickel affinity chromatography and gel filtration, to homogeneity Mus musculus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.8.2.2 DU-145 cell
-
 Homo sapiens
-
2.8.2.2 LNCaP cell
-
 Homo sapiens
-
2.8.2.2 LNCaP-C4-2 cell
-
 Homo sapiens
-
2.8.2.2 additional information androgen responsive cell lines LNCaP, VCaP, RWPE-1, and the castration nonresponsive line C4-2 generally express higher levels of SULT2B1b than AR- cell lines, such as PC-3 and DU-145 cells Homo sapiens
-
2.8.2.2 PC-3 cell
-
 Homo sapiens
-
2.8.2.2 prostate gland
-
 Homo sapiens
-
2.8.2.2 prostate gland cancer cell
-
 Homo sapiens
-
2.8.2.2 RWPE-1 cell
-
 Homo sapiens
-
2.8.2.2 VCaP cell
-
 Homo sapiens
-
2.8.2.14 liver liver mRNA expression profiles between wild-type mice under fed or 72 h fasted states using the mRNA FDD analysis and isolated a 910 bp partial cDNA fragment Mus musculus
-
2.8.2.14 additional information no detectable expression of mL-STL mRNA is found in other mouse tissues, including the stomach, small intestine, cecum, colon, kidney, heart, lung, spleen, brain, brown fat, white fat, smooth muscle, testis, and ovaries Mus musculus
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.8.2.14 155.85
-
 pH 5.5, 37°C, purified recombinant wild-type enzyme, substrate beta-muricholic acid Mus musculus
2.8.2.14 274.1
-
 pH 5.5, 37°C, purified recombinant wild-type enzyme, substrate ursodeoxycholic acid Mus musculus
2.8.2.14 485.1 720.7 pH 5.5, 37°C, purified recombinant wild-type enzyme, substrate taurocholic acid Mus musculus
2.8.2.14 579 652.3 pH 5.5, 37°C, purified recombinant wild-type enzyme, substrate cholic acid Mus musculus
2.8.2.14 649.9 960.1 pH 5.5, 37°C, purified recombinant wild-type enzyme, substrate glycocholic acid Mus musculus
2.8.2.14 854.4
-
 pH 5.5, 37°C, purified recombinant wild-type enzyme, substrate alpha-muricholic acid Mus musculus
2.8.2.14 1154.3 1287.4 pH 5.5, 37°C, purified recombinant wild-type enzyme, substrate chenodeoxycholic acid Mus musculus
2.8.2.14 1294.7
-
 pH 5.5, 37°C, purified recombinant wild-type enzyme, substrate taurochenodeoxycholic acid Mus musculus
2.8.2.14 1380.2
-
 pH 5.5, 37°C, purified recombinant wild-type enzyme, substrate glycochenodeoxycholic acid Mus musculus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.8.2.2 3'-phosphoadenylyl sulfate + cholesterol
-
 Homo sapiens adenosine 3',5'-bisphosphate + cholesterol 3-sulfate
-
 ?
2.8.2.14 3'-phosphoadenylyl sulfate + alpha-muricholic acid
-
 Mus musculus adenosine 3',5'-bisphosphate + alpha-muricholate 3-sulfate
-
 ?
2.8.2.14 3'-phosphoadenylyl sulfate + alpha-muricholic acid
-
 Mus musculus SV/129 adenosine 3',5'-bisphosphate + alpha-muricholate 3-sulfate
-
 ?
2.8.2.14 3'-phosphoadenylyl sulfate + beta-muricholic acid low activity Mus musculus adenosine 3',5'-bisphosphate + beta-muricholate 3-sulfate
-
 ?
2.8.2.14 3'-phosphoadenylyl sulfate + beta-muricholic acid low activity Mus musculus SV/129 adenosine 3',5'-bisphosphate + beta-muricholate 3-sulfate
-
 ?
2.8.2.14 3'-phosphoadenylyl sulfate + chenodeoxycholic acid
-
 Mus musculus adenosine 3',5'-bisphosphate + chenodeoxycholate 3-sulfate
-
 ?
2.8.2.14 3'-phosphoadenylyl sulfate + chenodeoxycholic acid
-
 Mus musculus SV/129 adenosine 3',5'-bisphosphate + chenodeoxycholate 3-sulfate
-
 ?
2.8.2.14 3'-phosphoadenylyl sulfate + cholic acid
-
 Mus musculus adenosine 3',5'-bisphosphate + cholate 3-sulfate
-
 ?
2.8.2.14 3'-phosphoadenylyl sulfate + cholic acid
-
 Mus musculus SV/129 adenosine 3',5'-bisphosphate + cholate 3-sulfate
-
 ?
2.8.2.14 3'-phosphoadenylyl sulfate + glycochenodeoxycholic acid
-
 Mus musculus adenosine 3',5'-bisphosphate + glycochenodeoxycholate 3-sulfate
-
 ?
2.8.2.14 3'-phosphoadenylyl sulfate + glycocholic acid
-
 Mus musculus adenosine 3',5'-bisphosphate + glycocholate 3-sulfate
-
 ?
2.8.2.14 3'-phosphoadenylyl sulfate + taurochenodeoxycholic acid
-
 Mus musculus adenosine 3',5'-bisphosphate + taurochenodeoxycholate 3-sulfate
-
 ?
2.8.2.14 3'-phosphoadenylyl sulfate + taurocholic acid
-
 Mus musculus adenosine 3',5'-bisphosphate + taurocholate 3-sulfate
-
 ?
2.8.2.14 3'-phosphoadenylyl sulfate + ursodeoxycholic acid low activity Mus musculus adenosine 3',5'-bisphosphate + ursodeoxycholate 3-sulfate
-
 ?
2.8.2.14 additional information recombinant His-tagged mL-STL protein preferentially catalyzes primary bile acid substrates. Low mL-STL-mediated SULT activity is observed with beta-muricholic acid (beta-MCA), and ursodeoxycholic acid (UDCA), whereas very low activity is observed in omega-muricholic (omega-MCA), deoxycholic acid, lithocholic acid, taurolithocholic acid sodium salt, androsterone, dehydroepiandrosterone (DHEA), pregnenolone, cholesterol, 22(S)-hydroxycholesterol, 22(R)-hydroxycholesterol, and corticosterone, substrate specificity, overview. His-tagged mL-STL protein displays relatively low substrate specificity toward the SULT1 prototype substrates examined Mus musculus ?
-
-
2.8.2.14 additional information recombinant His-tagged mL-STL protein preferentially catalyzes primary bile acid substrates. Low mL-STL-mediated SULT activity is observed with beta-muricholic acid (beta-MCA), and ursodeoxycholic acid (UDCA), whereas very low activity is observed in omega-muricholic (omega-MCA), deoxycholic acid, lithocholic acid, taurolithocholic acid sodium salt, androsterone, dehydroepiandrosterone (DHEA), pregnenolone, cholesterol, 22(S)-hydroxycholesterol, 22(R)-hydroxycholesterol, and corticosterone, substrate specificity, overview. His-tagged mL-STL protein displays relatively low substrate specificity toward the SULT1 prototype substrates examined Mus musculus SV/129 ?
-
-

Synonyms

EC Number Synonyms Comment Organism
2.8.2.2 cholesterol sulfotransferase
-
 Homo sapiens
2.8.2.2 SULT2B1b
-
 Homo sapiens
2.8.2.14 mL-STL
-
 Mus musculus
2.8.2.14 More cf. EC 2.8.2.1 Mus musculus
2.8.2.14 mouse liver-sulfotransferase-like
-
 Mus musculus
2.8.2.14 sulfotransferase
-
 Mus musculus
2.8.2.14 SULT2A8
-
 Mus musculus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.8.2.14 37
-
 assay at Mus musculus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.8.2.14 5.5
-
 recombinant enzyme Mus musculus

Expression

EC Number Organism Comment Expression
2.8.2.14 Mus musculus Wy-14,643 treatment downregulates mL-STL expression in a time- and PPARalpha-dependent manner. Two weeks of Wy-14,643 treatment result in dramatic suppression of mLSTL mRNA and protein expressions in the wild-type, but not in KO, mice, suggesting that the downregulation is mediated through the activation of PPARalpha upon Wy-14,643 treatment down
2.8.2.14 Mus musculus liver mRNA expression profiles between wild-type mice and PPARalpha-null mice under fed or 72 h fasted states using the mRNA FDD analysis and isolated a 910 bp partial cDNA fragment. Fasting has a time-dependent inhibition on the mL-STL protein expression in the KO mice, but not significantly in the wild-type mice additional information
2.8.2.14 Mus musculus PPARalpha is required to sustain Sult2a8 expression during fasting up

General Information

EC Number General Information Comment Organism
2.8.2.2 malfunction SULT2B1b knockdown induces cell death in prostate cancer cells. SULT2B1b knockdown increases the percentage of sub-G1 nuclei by cell-cycle analysis and significantly increased caspase-3 activity and PARP cleavage in LNCaP, VCaP, and C4-2 cells. Targeted knockdown of SULT2B1b impairs growth/viability of prostate cancer cells and induces apoptosis. LNCaP cells with SULT2B1b overexpression (hSULT2B1b vector) show a significant decrease in liver X receptor (LXR) activity and a trend of decreased transcription of downstream target gene, ATP-binding cassette (ABC)-G1. LXR activity in LNCaP cells has been demonstrated to be due to transcriptional activation of LXRbeta Homo sapiens
2.8.2.2 physiological function role of SULT2B1b in the growth and progression of cancer cells. Cholesterol sulfate accumulation correlates with SULT2B1b expression in prostate cancer cells and human prostate specimens. SULT2B1b activity modulates androgen receptor (AR) activity in prostate cancer cells, SULT2B1b is a regulator of AR activity and cell growth in prostate cancer Homo sapiens
2.8.2.14 evolution full-length mL-STL cDNA has alternative exonic splicing variants and shares high amino acid sequence homology to the SULT2A family. The mL-STL gene belongs to the Sult2a family of a superfamily of cytosolic SULTs Mus musculus
2.8.2.14 physiological function SULT2A8/mL-STL is a PPARalpha-regulated and 7alpha-hydroxylated bile acid-preferring cytosolic sulfotransferase, it is a liver-specific and male-dominant protein. PPARalpha modulates the homeostasis of bile acids during fasting being an essential regulator in bile acid biotransformation via sulfonation during fasting. The enzyme reveals a narrow sulfonating activity toward 7alpha-hydroxyl primary bile acids, including cholic acid, chenodeoxycholic acid, and alpha-muricholic acid, and thus may be the major hepatic bile acid sulfonating enzyme in mice Mus musculus