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Literature summary extracted from

  • Murakami, N.; Kotaka, A.; Isogai, S.; Ashida, K.; Nishimura, A.; Matsumura, K.; Hata, Y.; Ishida, H.; Takagi, H.
    Effects of a novel variant of the yeast gamma-glutamyl kinase Pro1 on its enzymatic activity and sake brewing (2020), J. Ind. Microbiol. Biotechnol., 47, 715-723 .
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.7.2.11 expressed in Escherichia coli BL21(DE3) cells Saccharomyces cerevisiae

Protein Variants

EC Number Protein Variants Comment Organism
2.7.2.11 I150T the mutant is greatly insensitive to feedback inhibition, leading to L-proline overproduction. The relative activity of the variant is 59% in the presence of 100 mM L-proline. The specific activity of the variant is slightly reduced compared with the wild type enzyme Saccharomyces cerevisiae
2.7.2.11 Q79H the mutation results in extreme desensitization to feedback inhibition by L-proline, leading to L-proline overproduction. The relative activity of the variant is 96% in the presence of 100 mM L-proline and 87% in the presence of 400 mM L-proline. The specific activity of the variant is slightly reduced compared with the wild type enzyme Saccharomyces cerevisiae

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.7.2.11 L-proline
-
 Saccharomyces cerevisiae

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.7.2.11 Mg2+ 10 mM used in assay conditions Saccharomyces cerevisiae

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.7.2.11 ATP + L-glutamate Saccharomyces cerevisiae
-
 ADP + L-glutamate 5-phosphate
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.7.2.11 Saccharomyces cerevisiae P32264
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.7.2.11 Ni-Sepharose column chromatography Saccharomyces cerevisiae

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.7.2.11 11
-
 mutant enzyme Q79H, at pH 7.4 and 30°C Saccharomyces cerevisiae
2.7.2.11 14.3
-
 mutant enzyme I150T, at pH 7.4 and 30°C Saccharomyces cerevisiae
2.7.2.11 14.3
-
 wild type enzyme, at pH 7.4 and 30°C Saccharomyces cerevisiae

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.2.11 ATP + L-glutamate
-
 Saccharomyces cerevisiae ADP + L-glutamate 5-phosphate
-
 ?

Synonyms

EC Number Synonyms Comment Organism
2.7.2.11 gamma-glutamyl kinase
-
 Saccharomyces cerevisiae
2.7.2.11 PRO1
-
 Saccharomyces cerevisiae

General Information

EC Number General Information Comment Organism
2.7.2.11 metabolism key enzyme in proline biosynthesis Saccharomyces cerevisiae