EC Number | Application | Comment | Organism |
---|---|---|---|
2.7.4.1 | synthesis | efficient synthesis of gamma-glutamyl compounds by co-expression of gamma-glutamylmethylamide synthetase and polyphosphate kinase in engineered Escherichia coli | Corynebacterium glutamicum |
6.3.4.12 | synthesis | efficient synthesis of gamma-glutamyl compounds by co-expression of gamma-glutamylmethylamide synthetase and polyphosphate kinase in engineered Escherichia coli | Methylovorus mays |
EC Number | Metals/Ions | Comment | Organism | Structure |
---|---|---|---|---|
2.7.4.1 | Co2+ | activates | Corynebacterium glutamicum | |
2.7.4.1 | Mg2+ | activates | Corynebacterium glutamicum | |
2.7.4.1 | Mn2+ | activates | Corynebacterium glutamicum | |
2.7.4.1 | Zn2+ | activates | Corynebacterium glutamicum | |
6.3.4.12 | Co2+ | activates | Methylovorus mays | |
6.3.4.12 | Mg2+ | activates | Methylovorus mays | |
6.3.4.12 | Mn2+ | activates | Methylovorus mays | |
6.3.4.12 | additional information | no activation by Zn2+ and Ca2+ | Methylovorus mays |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
2.7.4.1 | 38000 | - |
SDS-PAGE | Corynebacterium glutamicum |
6.3.4.12 | 49000 | - |
SDS-PAGE | Methylovorus mays |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
2.7.4.1 | Corynebacterium glutamicum | ANU33171.1 | - |
- |
6.3.4.12 | Methylovorus mays | A9ZPH9 | - |
- |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
2.7.4.1 | ADP + phosphate(6) | i.e. hexametaphosphate | Corynebacterium glutamicum | ATP + phosphate(5) | - |
? | |
6.3.4.12 | ATP + L-glutamate + ethylamine | - |
Methylovorus mays | ADP + phosphate + N5-ethyl-L-glutamine | - |
? |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
2.7.4.1 | PPK | - |
Corynebacterium glutamicum |
6.3.4.12 | gamma-Glutamylmethylamide synthetase | - |
Methylovorus mays |
6.3.4.12 | GMAS | - |
Methylovorus mays |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
2.7.4.1 | 40 | - |
- |
Corynebacterium glutamicum |
6.3.4.12 | 45 | - |
- |
Methylovorus mays |
EC Number | Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|---|
2.7.4.1 | 30 | 50 | 30°C: 83% of maximal activity, 50°C: 79% of maximal activity | Corynebacterium glutamicum |
6.3.4.12 | 35 | 55 | 35°C: about 75% of maximal activity, 55°C: about 60% of maximal activity | Methylovorus mays |
EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|---|
2.7.4.1 | 35 | - |
3 h, 10% loss of activity | Corynebacterium glutamicum |
2.7.4.1 | 40 | - |
3 h, 22% loss of activity | Corynebacterium glutamicum |
6.3.4.12 | 30 | - |
3 h, less than 10% loss of activity | Methylovorus mays |
6.3.4.12 | 50 | - |
3 h, immediate loss of activity | Methylovorus mays |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
2.7.4.1 | 9 | - |
- |
Corynebacterium glutamicum |
6.3.4.12 | 8 | - |
- |
Methylovorus mays |
EC Number | pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|---|
6.3.4.12 | 6 | 9 | pH 6.0: about 35% of maximal activity, pH 7.0, about 90% of maximal activity, pH 9.0: about 20% of maximal activity | Methylovorus mays |
6.3.4.12 | 6 | 10 | pH 6.0: about 65% of maximal activity, pH 10.0: about 45% of maximal activity | Methylovorus mays |
EC Number | pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|---|
2.7.4.1 | 7 | - |
24 h, 20% loss of activity | Corynebacterium glutamicum |
2.7.4.1 | 9 | - |
24 h, 50% loss of activity | Corynebacterium glutamicum |
6.3.4.12 | 8 | - |
24 h, 40% loss of activity | Methylovorus mays |
6.3.4.12 | 9 | - |
24 h, complete inactivation | Methylovorus mays |