Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Pan, X.; Yu, J.; Du, Q.; Zeng, S.; Liu, J.; Jiao, Q.; Zhang, H.
    Efficient synthesis of gamma-glutamyl compounds by co-expression of gamma-glutamylmethylamide synthetase and polyphosphate kinase in engineered Escherichia coli (2020), J. Ind. Microbiol. Biotechnol., 47, 573-583 .
    View publication on PubMed

Application

EC Number Application Comment Organism
2.7.4.1 synthesis efficient synthesis of gamma-glutamyl compounds by co-expression of gamma-glutamylmethylamide synthetase and polyphosphate kinase in engineered Escherichia coli Corynebacterium glutamicum
6.3.4.12 synthesis efficient synthesis of gamma-glutamyl compounds by co-expression of gamma-glutamylmethylamide synthetase and polyphosphate kinase in engineered Escherichia coli Methylovorus mays

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.7.4.1 Co2+ activates Corynebacterium glutamicum
2.7.4.1 Mg2+ activates Corynebacterium glutamicum
2.7.4.1 Mn2+ activates Corynebacterium glutamicum
2.7.4.1 Zn2+ activates Corynebacterium glutamicum
6.3.4.12 Co2+ activates Methylovorus mays
6.3.4.12 Mg2+ activates Methylovorus mays
6.3.4.12 Mn2+ activates Methylovorus mays
6.3.4.12 additional information no activation by Zn2+ and Ca2+ Methylovorus mays

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.7.4.1 38000
-
SDS-PAGE Corynebacterium glutamicum
6.3.4.12 49000
-
SDS-PAGE Methylovorus mays

Organism

EC Number Organism UniProt Comment Textmining
2.7.4.1 Corynebacterium glutamicum ANU33171.1
-
-
6.3.4.12 Methylovorus mays A9ZPH9
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.4.1 ADP + phosphate(6) i.e. hexametaphosphate Corynebacterium glutamicum ATP + phosphate(5)
-
?
6.3.4.12 ATP + L-glutamate + ethylamine
-
Methylovorus mays ADP + phosphate + N5-ethyl-L-glutamine
-
?

Synonyms

EC Number Synonyms Comment Organism
2.7.4.1 PPK
-
Corynebacterium glutamicum
6.3.4.12 gamma-Glutamylmethylamide synthetase
-
Methylovorus mays
6.3.4.12 GMAS
-
Methylovorus mays

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.7.4.1 40
-
-
Corynebacterium glutamicum
6.3.4.12 45
-
-
Methylovorus mays

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
2.7.4.1 30 50 30°C: 83% of maximal activity, 50°C: 79% of maximal activity Corynebacterium glutamicum
6.3.4.12 35 55 35°C: about 75% of maximal activity, 55°C: about 60% of maximal activity Methylovorus mays

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
2.7.4.1 35
-
3 h, 10% loss of activity Corynebacterium glutamicum
2.7.4.1 40
-
3 h, 22% loss of activity Corynebacterium glutamicum
6.3.4.12 30
-
3 h, less than 10% loss of activity Methylovorus mays
6.3.4.12 50
-
3 h, immediate loss of activity Methylovorus mays

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.7.4.1 9
-
-
Corynebacterium glutamicum
6.3.4.12 8
-
-
Methylovorus mays

pH Range

EC Number pH Minimum pH Maximum Comment Organism
6.3.4.12 6 9 pH 6.0: about 35% of maximal activity, pH 7.0, about 90% of maximal activity, pH 9.0: about 20% of maximal activity Methylovorus mays
6.3.4.12 6 10 pH 6.0: about 65% of maximal activity, pH 10.0: about 45% of maximal activity Methylovorus mays

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
2.7.4.1 7
-
24 h, 20% loss of activity Corynebacterium glutamicum
2.7.4.1 9
-
24 h, 50% loss of activity Corynebacterium glutamicum
6.3.4.12 8
-
24 h, 40% loss of activity Methylovorus mays
6.3.4.12 9
-
24 h, complete inactivation Methylovorus mays