Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Kim, S.; Jo, S.; Kim, M.S.; Shin, D.H.
    A study of Rose Bengal against a 2-keto-3-deoxy-D-manno-octulosonate cytidylyltransferase as an antibiotic candidate (2020), J. Enzyme Inhib. Med. Chem., 35, 1414-1421 .
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.7.7.38 expressed in Escherichia coli BL21(DE3) cells Burkholderia thailandensis
2.7.7.38 expressed in Escherichia coli BL21(DE3) cells Chlamydia psittaci
2.7.7.38 expressed in Escherichia coli BL21(DE3) cells Burkholderia pseudomallei
2.7.7.38 expressed in Escherichia coli BL21(DE3) cells Pseudomonas aeruginosa

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.7.7.38 4,5,6,7-tetrachloro-2',4',5',7'-tetraiodofluorescein Rose Bengal, competitive with 2-keto-3-deoxy-D-manno-octulosonate but non-competitive against CTP Burkholderia pseudomallei
2.7.7.38 4,5,6,7-tetrachloro-2',4',5',7'-tetraiodofluorescein Rose Bengal, competitive with 2-keto-3-deoxy-D-manno-octulosonate but non-competitive against CTP Burkholderia thailandensis
2.7.7.38 4,5,6,7-tetrachloro-2',4',5',7'-tetraiodofluorescein Rose Bengal, competitive with 2-keto-3-deoxy-D-manno-octulosonate but non-competitive against CTP Pseudomonas aeruginosa
2.7.7.38 additional information not inhibited by 4,5,6,7-tetrachloro-2',4',5',7'-tetraiodofluorescein Chlamydia psittaci

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.7.7.38 0.056
-
 3-deoxy-D-manno-octulosonate at pH 7.5 and 25°C Burkholderia thailandensis
2.7.7.38 0.059
-
 3-deoxy-D-manno-octulosonate at pH 7.5 and 25°C Burkholderia pseudomallei
2.7.7.38 0.061
-
 3-deoxy-D-manno-octulosonate at pH 7.5 and 25°C Pseudomonas aeruginosa
2.7.7.38 0.0983
-
 CTP at pH 7.5 and 25°C Pseudomonas aeruginosa
2.7.7.38 0.1111
-
 CTP at pH 7.5 and 25°C Burkholderia pseudomallei
2.7.7.38 0.1239
-
 CTP at pH 7.5 and 25°C Burkholderia thailandensis

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.7.7.38 Mg2+ 10 mM used in assay conditions Burkholderia thailandensis
2.7.7.38 Mg2+ 10 mM used in assay conditions Chlamydia psittaci
2.7.7.38 Mg2+ 10 mM used in assay conditions Burkholderia pseudomallei
2.7.7.38 Mg2+ 10 mM used in assay conditions Pseudomonas aeruginosa

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.7.7.38 CTP + 3-deoxy-D-manno-octulosonate Burkholderia thailandensis
-
 diphosphate + CMP-3-deoxy-D-manno-octulosonate
-
 ?
2.7.7.38 CTP + 3-deoxy-D-manno-octulosonate Chlamydia psittaci
-
 diphosphate + CMP-3-deoxy-D-manno-octulosonate
-
 ?
2.7.7.38 CTP + 3-deoxy-D-manno-octulosonate Burkholderia pseudomallei
-
 diphosphate + CMP-3-deoxy-D-manno-octulosonate
-
 ?
2.7.7.38 CTP + 3-deoxy-D-manno-octulosonate Pseudomonas aeruginosa
-
 diphosphate + CMP-3-deoxy-D-manno-octulosonate
-
 ?
2.7.7.38 CTP + 3-deoxy-D-manno-octulosonate Burkholderia pseudomallei K96243
-
 diphosphate + CMP-3-deoxy-D-manno-octulosonate
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.7.7.38 Burkholderia pseudomallei Q63WL5
-
-
2.7.7.38 Burkholderia pseudomallei K96243 Q63WL5
-
-
2.7.7.38 Burkholderia thailandensis
-
-
-
2.7.7.38 Chlamydia psittaci A0A2D2D9V4
-
-
2.7.7.38 Pseudomonas aeruginosa A0A0C7D0B2
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.7.7.38 Hi-trap Q column chromatography Burkholderia thailandensis
2.7.7.38 Hi-trap Q column chromatography Chlamydia psittaci
2.7.7.38 Hi-trap Q column chromatography Burkholderia pseudomallei
2.7.7.38 Hi-trap Q column chromatography Pseudomonas aeruginosa

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.7.38 CTP + 3-deoxy-D-manno-octulosonate
-
 Burkholderia thailandensis diphosphate + CMP-3-deoxy-D-manno-octulosonate
-
 ?
2.7.7.38 CTP + 3-deoxy-D-manno-octulosonate
-
 Chlamydia psittaci diphosphate + CMP-3-deoxy-D-manno-octulosonate
-
 ?
2.7.7.38 CTP + 3-deoxy-D-manno-octulosonate
-
 Burkholderia pseudomallei diphosphate + CMP-3-deoxy-D-manno-octulosonate
-
 ?
2.7.7.38 CTP + 3-deoxy-D-manno-octulosonate
-
 Pseudomonas aeruginosa diphosphate + CMP-3-deoxy-D-manno-octulosonate
-
 ?
2.7.7.38 CTP + 3-deoxy-D-manno-octulosonate
-
 Burkholderia pseudomallei K96243 diphosphate + CMP-3-deoxy-D-manno-octulosonate
-
 ?

Subunits

EC Number Subunits Comment Organism
2.7.7.38 ? x * 29500, calculated from amino acid sequence Pseudomonas aeruginosa
2.7.7.38 ? x * 28500, calculated from amino acid sequence Burkholderia thailandensis
2.7.7.38 ? x * 30400, calculated from amino acid sequence Chlamydia psittaci
2.7.7.38 ? x * 30000, calculated from amino acid sequence Burkholderia pseudomallei

Synonyms

EC Number Synonyms Comment Organism
2.7.7.38 2-keto-3-deoxy-D-manno-octulosonate cytidylyltransferase
-
 Burkholderia thailandensis
2.7.7.38 2-keto-3-deoxy-D-manno-octulosonate cytidylyltransferase
-
 Chlamydia psittaci
2.7.7.38 2-keto-3-deoxy-D-manno-octulosonate cytidylyltransferase
-
 Burkholderia pseudomallei
2.7.7.38 2-keto-3-deoxy-D-manno-octulosonate cytidylyltransferase
-
 Pseudomonas aeruginosa
2.7.7.38 KdsB
-
 Burkholderia thailandensis
2.7.7.38 KdsB
-
 Chlamydia psittaci
2.7.7.38 KdsB
-
 Burkholderia pseudomallei
2.7.7.38 KdsB
-
 Pseudomonas aeruginosa

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
2.7.7.38 76.8
-
 melting temperature of the native enzyme Burkholderia pseudomallei

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2.7.7.38 0.076
-
 3-deoxy-D-manno-octulosonate at pH 7.5 and 25°C Pseudomonas aeruginosa
2.7.7.38 0.088
-
 3-deoxy-D-manno-octulosonate at pH 7.5 and 25°C Burkholderia pseudomallei
2.7.7.38 0.116
-
 3-deoxy-D-manno-octulosonate at pH 7.5 and 25°C Burkholderia thailandensis
2.7.7.38 0.132
-
 CTP at pH 7.5 and 25°C Pseudomonas aeruginosa
2.7.7.38 0.142
-
 CTP at pH 7.5 and 25°C Burkholderia thailandensis
2.7.7.38 0.167
-
 CTP at pH 7.5 and 25°C Burkholderia pseudomallei

IC50 Value

EC Number IC50 Value IC50 Value Maximum Comment Organism Inhibitor Structure
2.7.7.38 0.00531
-
 at pH 7.5 and 25°C Burkholderia pseudomallei 4,5,6,7-tetrachloro-2',4',5',7'-tetraiodofluorescein
2.7.7.38 0.00724
-
 at pH 7.5 and 25°C Burkholderia thailandensis 4,5,6,7-tetrachloro-2',4',5',7'-tetraiodofluorescein
2.7.7.38 0.01466
-
 at pH 7.5 and 25°C Pseudomonas aeruginosa 4,5,6,7-tetrachloro-2',4',5',7'-tetraiodofluorescein