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Literature summary extracted from

  • Dai, L.; Li, J.; Yao, P.; Zhu, Y.; Men, Y.; Zeng, Y.; Yang, J.; Sun, Y.
    Exploiting the aglycon promiscuity of glycosyltransferase Bs-YjiC from Bacillus subtilis and its application in synthesis of glycosides (2017), J. Biotechnol., 248, 69-76 .
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.4.1.384 expressed in Escherichia coli BL21(DE3) cells Bacillus subtilis

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.4.1.384 Cu2+ less than 20% residual activity at 10 mM Bacillus subtilis
2.4.1.384 Ni2+ less than 10% residual activity at 10 mM Bacillus subtilis
2.4.1.384 Zn2+ less than 10% residual activity at 10 mM Bacillus subtilis

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.4.1.384 0.0194
-
 apigenin at pH 7.0 and 30°C Bacillus subtilis
2.4.1.384 0.0226
-
 genistein at pH 7.0 and 30°C Bacillus subtilis

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.4.1.384 Ca2+ the enzyme activity is significantly improved in the presence of Ca2+ Bacillus subtilis
2.4.1.384 Mg2+ the enzyme activity is significantly improved in the presence of Mg2+ Bacillus subtilis
2.4.1.384 Mn2+ the enzyme activity is significantly improved in the presence of Mn2+ with the optimal activity at 10 mM Bacillus subtilis

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.4.1.384 an NDP-glycose + an acceptor Bacillus subtilis
-
 a glycosylated acceptor + NDP
-
 ?
2.4.1.384 an NDP-glycose + an acceptor Bacillus subtilis 168
-
 a glycosylated acceptor + NDP
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.4.1.384 Bacillus subtilis O34539
-
-
2.4.1.384 Bacillus subtilis 168 O34539
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.4.1.384 Ni-NTA column chromatography Bacillus subtilis

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.4.1.384 an NDP-glycose + an acceptor
-
 Bacillus subtilis a glycosylated acceptor + NDP
-
 ?
2.4.1.384 an NDP-glycose + an acceptor
-
 Bacillus subtilis 168 a glycosylated acceptor + NDP
-
 ?
2.4.1.384 UDP-alpha-D-glucose + apigenin
-
 Bacillus subtilis apigenin 4'-O-glucoside + apigenin 7-O-glucoside + apigenin 4',7-O-diglucoside + UDP
-
 ?
2.4.1.384 UDP-alpha-D-glucose + apigenin
-
 Bacillus subtilis 168 apigenin 4'-O-glucoside + apigenin 7-O-glucoside + apigenin 4',7-O-diglucoside + UDP
-
 ?
2.4.1.384 UDP-alpha-D-glucose + genistein
-
 Bacillus subtilis genistein 4'-O-glucoside + genistein 7-O-glucoside + genistein 4',7-O-diglucoside + UDP
-
 ?
2.4.1.384 UDP-alpha-D-glucose + genistein
-
 Bacillus subtilis 168 genistein 4'-O-glucoside + genistein 7-O-glucoside + genistein 4',7-O-diglucoside + UDP
-
 ?
2.4.1.384 UDP-alpha-D-glucose + oleanic acid
-
 Bacillus subtilis oleanic acid 3-O-beta-D-glucoside + UDP
-
 ?
2.4.1.384 UDP-alpha-D-glucose + oleanic acid
-
 Bacillus subtilis 168 oleanic acid 3-O-beta-D-glucoside + UDP
-
 ?
2.4.1.384 UDP-alpha-D-glucose + pterostilbene
-
 Bacillus subtilis pterostilbene 4'-O-beta-glucopyranoside + UDP
-
 ?
2.4.1.384 UDP-alpha-D-glucose + pterostilbene
-
 Bacillus subtilis 168 pterostilbene 4'-O-beta-glucopyranoside + UDP
-
 ?

Subunits

EC Number Subunits Comment Organism
2.4.1.384 ? x * 62000, SDS-PAGE Bacillus subtilis

Synonyms

EC Number Synonyms Comment Organism
2.4.1.384 macrolide glycosyltransferase
-
 Bacillus subtilis
2.4.1.384 MGT
-
 Bacillus subtilis
2.4.1.384 YjiC
-
 Bacillus subtilis

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
2.4.1.384 30 40 high activity within the temperature range of 30-40°C Bacillus subtilis

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2.4.1.384 1.6
-
 genistein at pH 7.0 and 30°C Bacillus subtilis
2.4.1.384 1.9
-
 apigenin at pH 7.0 and 30°C Bacillus subtilis

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.4.1.384 8
-
-
 Bacillus subtilis

pH Range

EC Number pH Minimum pH Maximum Comment Organism
2.4.1.384 6 9 the enzyme displays at least 60% of its maximal activity between pH 6.0 and 9.0 Bacillus subtilis

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
2.4.1.384 72
-
 genistein at pH 7.0 and 30°C Bacillus subtilis
2.4.1.384 98
-
 apigenin at pH 7.0 and 30°C Bacillus subtilis