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Literature summary extracted from

  • Shakir, N.; Bibi, T.; Aslam, M.; Rashid, N.
    Biochemical characterization of a highly active ADP-dependent phosphofructokinase from Thermococcus kodakarensis (2020), J. Biosci. Bioeng., 129, 6-15 .
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
2.7.1.11 phosphoenol pyruvate slightly enhances activity Thermococcus kodakarensis
2.7.1.146 phosphoenol pyruvate slight activation Thermococcus kodakarensis
2.7.1.146 phosphoenol pyruvate slightly enhances activity Thermococcus kodakarensis

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.7.1.11 expressed in Escherichia coli Thermococcus kodakarensis
2.7.1.146 expressed in Escherichia coli Thermococcus kodakarensis
2.7.1.146 gene pfkC, sequence comparisons, recombinant expression in Escherichia coli strain BL21-CodonPlus(DE3)-RIL as soluble and active enzyme Thermococcus kodakarensis

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.7.1.11 AMP competitive Thermococcus kodakarensis
2.7.1.11 ATP competitive Thermococcus kodakarensis
2.7.1.11 EDTA 5 mM, complete loss of activity Thermococcus kodakarensis
2.7.1.146 adenosine slight inhibition Thermococcus kodakarensis
2.7.1.146 AMP competitive Thermococcus kodakarensis
2.7.1.146 ATP competitive Thermococcus kodakarensis
2.7.1.146 citrate slight inhibition Thermococcus kodakarensis
2.7.1.146 EDTA 5 mM, complete loss of activity Thermococcus kodakarensis
2.7.1.146 additional information addition of pyruvate does not affect the activity Thermococcus kodakarensis
2.7.1.146 Zn2+ slightly inhibitory Thermococcus kodakarensis

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.7.1.11 0.32
-
 ADP pH 7.5, 90°C, 5 mM Co2+ Thermococcus kodakarensis
2.7.1.11 0.55
-
 D-fructose 6-phosphate pH 7.5, 50°C, 5 mM Mg2+ Thermococcus kodakarensis
2.7.1.11 0.58
-
 D-fructose 6-phosphate pH 7.5, 50°C, 5 mM Co2+ Thermococcus kodakarensis
2.7.1.11 0.7
-
 D-fructose 1,6-bisphosphate pH 7.5, 50°C, 5 mM Mg2+ Thermococcus kodakarensis
2.7.1.11 0.74
-
 ADP pH 7.5, 50°C, 5 mM Mg2+ Thermococcus kodakarensis
2.7.1.11 0.74
-
 D-fructose 6-phosphate pH 7.5, 90°C, 5 mM Co2+ Thermococcus kodakarensis
2.7.1.11 0.86
-
 ADP pH 7.5, 50°C, 5 mM Co2+ Thermococcus kodakarensis
2.7.1.11 1.57
-
 AMP pH 7.5, 50°C, 5 mM Mg2+ Thermococcus kodakarensis
2.7.1.11 90
-
 D-glucose pH 7.5, 50°C, 5 mM Mg2+ Thermococcus kodakarensis
2.7.1.146 additional information
-
 additional information Michaelis-Menten kinetic analysis of the phosphofructokinase activity, overview Thermococcus kodakarensis
2.7.1.146 0.32
-
 ADP pH 7.5, 90°C, 5 mM Co2+ Thermococcus kodakarensis
2.7.1.146 0.32
-
 ADP pH 7.5, 90°C, recombinant enzyme, in presence of Co2+, substrates ADP and D-fructose 6-phosphate Thermococcus kodakarensis
2.7.1.146 0.55
-
 D-fructose 6-phosphate pH 7.5, 50°C, 5 mM Mg2+ Thermococcus kodakarensis
2.7.1.146 0.55
-
 D-fructose 6-phosphate pH 7.5, 50°C, recombinant enzyme, in presence of Mg2+, substrates ADP and D-fructose 6-phosphate Thermococcus kodakarensis
2.7.1.146 0.58
-
 D-fructose 6-phosphate pH 7.5, 50°C, 5 mM Co2+ Thermococcus kodakarensis
2.7.1.146 0.58
-
 D-fructose 6-phosphate pH 7.5, 50°C, recombinant enzyme, in presence of Co2+, substrates ADP and D-fructose 6-phosphate Thermococcus kodakarensis
2.7.1.146 0.7
-
 D-fructose 1,6-bisphosphate pH 7.5, 50°C, 5 mM Mg2+ Thermococcus kodakarensis
2.7.1.146 0.74
-
 ADP pH 7.5, 50°C, 5 mM Mg2+ Thermococcus kodakarensis
2.7.1.146 0.74
-
 ADP pH 7.5, 50°C, recombinant enzyme, in presence of Mg2+, substrates ADP and D-fructose 6-phosphate Thermococcus kodakarensis
2.7.1.146 0.74
-
 D-fructose 6-phosphate pH 7.5, 90°C, 5 mM Co2+ Thermococcus kodakarensis
2.7.1.146 0.74
-
 D-fructose 6-phosphate pH 7.5, 90°C, recombinant enzyme, in presence of Co2+, substrates ADP and D-fructose 6-phosphate Thermococcus kodakarensis
2.7.1.146 0.86
-
 ADP pH 7.5, 50°C, 5 mM Co2+ Thermococcus kodakarensis
2.7.1.146 0.86
-
 ADP pH 7.5, 50°C, recombinant enzyme, in presence of Co2+, substrates ADP and D-fructose 6-phosphate Thermococcus kodakarensis
2.7.1.146 1.57
-
 AMP pH 7.5, 50°C, 5 mM Mg2+ Thermococcus kodakarensis
2.7.1.146 90
-
 D-glucose pH 7.5, 50°C, 5 mM Mg2+ Thermococcus kodakarensis
2.7.1.146 90
-
 D-glucose pH 7.5, 50°C, recombinant enzyme, in presence of Mg2+, substrates ADP and D-glucose Thermococcus kodakarensis

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.7.1.11 Co2+ metal-ion dependent enzyme, highest activity (5.09 mM/min*mg) in presence of Co2+ followed by Mg2+ (3.28 mM/min*mg) at 90°C and pH 7.5 Thermococcus kodakarensis
2.7.1.11 Mg2+ metal-ion dependent enzyme, highest activity (5.09 mM/min*mg) in presence of Co2+ followed by Mg2+ (3.28 mM/min*mg) at 90°C and pH 7.5 Thermococcus kodakarensis
2.7.1.11 Mn2+ dependent on divalent metal cations. Highest activity in the presence of Co2+ (100%) followed by Mg2+ (64%), Mn2+ (64%) and Ni2+ (34%) Thermococcus kodakarensis
2.7.1.11 Ni2+ dependent on divalent metal cations. Highest activity in the presence of Co2+ (100%) followed by Mg2+ (64%), Mn2+ (64%) and Ni2+ (34%) Thermococcus kodakarensis
2.7.1.146 Ca2+ slight activation Thermococcus kodakarensis
2.7.1.146 Cd2+ slight activation Thermococcus kodakarensis
2.7.1.146 Co2+ best activating divalent cation Thermococcus kodakarensis
2.7.1.146 Co2+ metal-ion dependent enzyme, highest activity (5.09 mM/min*mg) in presence of Co2+ followed by Mg2+ (3.28 mM/min*mg) at 90°C and pH 7.5 Thermococcus kodakarensis
2.7.1.146 Mg2+ activates to 64% compared to Co2+ Thermococcus kodakarensis
2.7.1.146 Mg2+ metal-ion dependent enzyme, highest activity (5.09 mM/min*mg) in presence of Co2+ followed by Mg2+ (3.28 mM/min*mg) at 90°C and pH 7.5 Thermococcus kodakarensis
2.7.1.146 Mn2+ activates to 64% compared to Co2+ Thermococcus kodakarensis
2.7.1.146 Mn2+ dependent on divalent metal cations. Highest activity in the presence of Co2+ (100%) followed by Mg2+ (64%), Mn2+ (64%) and Ni2+ (34%) Thermococcus kodakarensis
2.7.1.146 additional information the phosphofructokinase activity of the enzyme is metal ion-dependent, the highest activity is found in the presence of Co2+, followed by Mg2+ at 90°C and pH 7.5. 18% of maximal activity is shown in absence of metal ions Thermococcus kodakarensis
2.7.1.146 Ni2+ activates to 34% compared to Co2+ Thermococcus kodakarensis
2.7.1.146 Ni2+ dependent on divalent metal cations. Highest activity in the presence of Co2+ (100%) followed by Mg2+ (64%), Mn2+ (64%) and Ni2+ (34%) Thermococcus kodakarensis

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.7.1.11 52000
-
 SDS-PAGE Thermococcus kodakarensis
2.7.1.146 52000
-
 SDS-PAGE Thermococcus kodakarensis

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.7.1.11 ADP + D-fructose 1,6-bisphosphate Thermococcus kodakarensis
-
 ATP + D-fructose 6-phosphate
-
 r
2.7.1.11 ATP + D-fructose 6-phosphate Thermococcus kodakarensis
-
 ADP + D-fructose 1,6-bisphosphate
-
 r
2.7.1.146 ADP + D-fructose 1,6-bisphosphate Thermococcus kodakarensis
-
 ATP + D-fructose 6-phosphate
-
 r
2.7.1.146 ADP + D-fructose 6-phosphate Thermococcus kodakarensis
-
 AMP + D-fructose 1,6-bisphosphate
-
 r
2.7.1.146 ADP + D-fructose 6-phosphate Thermococcus kodakarensis JCM 12380
-
 AMP + D-fructose 1,6-bisphosphate
-
 r
2.7.1.146 ADP + D-fructose 6-phosphate Thermococcus kodakarensis ATCC BAA-918
-
 AMP + D-fructose 1,6-bisphosphate
-
 r
2.7.1.146 ATP + D-fructose 6-phosphate Thermococcus kodakarensis
-
 ADP + D-fructose 1,6-bisphosphate
-
 r
2.7.1.146 ATP + D-fructose 6-phosphate Thermococcus kodakarensis JCM 12380
-
 ADP + D-fructose 1,6-bisphosphate
-
 r
2.7.1.146 ATP + D-fructose 6-phosphate Thermococcus kodakarensis ATCC BAA-918
-
 ADP + D-fructose 1,6-bisphosphate
-
 r

Organism

EC Number Organism UniProt Comment Textmining
2.7.1.11 Thermococcus kodakarensis Q5JD05
-
-
2.7.1.11 Thermococcus kodakarensis ATCC BAA-918 Q5JD05
-
-
2.7.1.146 Thermococcus kodakarensis Q5JD05
-
-
2.7.1.146 Thermococcus kodakarensis Q5JD05 Pyrococcus kodakaraensis strain KOD1
-
2.7.1.146 Thermococcus kodakarensis ATCC BAA-918 Q5JD05
-
-
2.7.1.146 Thermococcus kodakarensis ATCC BAA-918 Q5JD05 Pyrococcus kodakaraensis strain KOD1
-
2.7.1.146 Thermococcus kodakarensis JCM 12380 Q5JD05 Pyrococcus kodakaraensis strain KOD1
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.7.1.11
-
 Thermococcus kodakarensis
2.7.1.146
-
 Thermococcus kodakarensis
2.7.1.146 recombinant enzyme 3.5fold from Escherichia coli strain BL21-CodonPlus(DE3)-RIL by heat treatment at 80°C for 30 min, anion exchange chromatography, and dialysis, followed by hydrophobic interaction chromatography, and again dialysis Thermococcus kodakarensis

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.7.1.11 5.09
-
 substrate: , pH 7.5, 50°C Thermococcus kodakarensis
2.7.1.11 19.5
-
 substrate: ribose 5-phosphate, pH 7.5, 50°C Thermococcus kodakarensis
2.7.1.11 46
-
 substrate: adenosine, pH 7.5, 50°C Thermococcus kodakarensis
2.7.1.11 66
-
 substrate: 2-deoxyadenosine, pH 7.5, 50°C Thermococcus kodakarensis
2.7.1.11 70
-
 substrate: thymidine, pH 7.5, 50°C Thermococcus kodakarensis
2.7.1.11 86
-
 substrate: D-glucose, pH 7.5, 50°C Thermococcus kodakarensis
2.7.1.146 5.09
-
 substrate: , pH 7.5, 50°C Thermococcus kodakarensis
2.7.1.146 19.5
-
 purified recombinant enzyme, pH 7.5, 50°C, in presence of Co2+, substrates ADP and ribose 5-phosphate Thermococcus kodakarensis
2.7.1.146 19.5
-
 substrate: ribose 5-phosphate, pH 7.5, 50°C Thermococcus kodakarensis
2.7.1.146 46
-
 purified recombinant enzyme, pH 7.5, 50°C, in presence of Co2+, substrates ADP and adenosine Thermococcus kodakarensis
2.7.1.146 46
-
 substrate: adenosine, pH 7.5, 50°C Thermococcus kodakarensis
2.7.1.146 66
-
 purified recombinant enzyme, pH 7.5, 50°C, in presence of Co2+, substrates ADP and 2-deoxyadenosine Thermococcus kodakarensis
2.7.1.146 66
-
 substrate: 2-deoxyadenosine, pH 7.5, 50°C Thermococcus kodakarensis
2.7.1.146 70
-
 purified recombinant enzyme, pH 7.5, 50°C, in presence of Co2+, substrates ADP and thymidine Thermococcus kodakarensis
2.7.1.146 70
-
 substrate: thymidine, pH 7.5, 50°C Thermococcus kodakarensis
2.7.1.146 86
-
 purified recombinant enzyme, pH 7.5, 50°C, in presence of Co2+, substrates ADP and D-glucose, reaction of EC 2.7.1.147 Thermococcus kodakarensis
2.7.1.146 86
-
 substrate: D-glucose, pH 7.5, 50°C Thermococcus kodakarensis
2.7.1.146 3280
-
 purified recombinant enzyme, pH 7.5, 50°C, in presence of Mg2+, substrates ADP and D-fructose 6-phosphate Thermococcus kodakarensis
2.7.1.146 5090
-
 purified recombinant enzyme, pH 7.5, 50°C, in presence of Co2+, substrates ADP and D-fructose 6-phosphate Thermococcus kodakarensis

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.1.11 ADP + 2'-deoxyadenosine
-
 Thermococcus kodakarensis AMP + ?
-
 ?
2.7.1.11 ADP + adenosine
-
 Thermococcus kodakarensis AMP + ?
-
 ?
2.7.1.11 ADP + D-fructose 1,6-bisphosphate
-
 Thermococcus kodakarensis ATP + D-fructose 6-phosphate
-
 r
2.7.1.11 ADP + D-fructose 6-phosphate
-
 Thermococcus kodakarensis AMP + D-fructose 1,6-bisphosphate
-
 r
2.7.1.11 ADP + D-glucose
-
 Thermococcus kodakarensis AMP + D-glucose 6-phosphate
-
 ?
2.7.1.11 ADP + ribose 5-phosphate
-
 Thermococcus kodakarensis AMP + ?
-
 ?
2.7.1.11 ADP + thymidine
-
 Thermococcus kodakarensis AMP + ?
-
 ?
2.7.1.11 AMP + D-fructose 1,6-bisphosphate
-
 Thermococcus kodakarensis ADP + D-fructose 6-phosphate
-
 r
2.7.1.11 ATP + D-fructose 6-phosphate
-
 Thermococcus kodakarensis ADP + D-fructose 1,6-bisphosphate
-
 r
2.7.1.11 ATP + D-fructose 6-phosphate 20% phosphofructokinase activity is observed in the presence of 2 mM ATP compared to 100% in the presence of equimolar ADP Thermococcus kodakarensis ADP + D-fructose 1,6-bisphosphate
-
 r
2.7.1.146 ADP + 2'-deoxyadenosine
-
 Thermococcus kodakarensis AMP + ?
-
 ?
2.7.1.146 ADP + 2'-deoxyadenosine
-
 Thermococcus kodakarensis AMP + dAMP
-
 r
2.7.1.146 ADP + adenosine
-
 Thermococcus kodakarensis 2 AMP
-
 r
2.7.1.146 ADP + adenosine
-
 Thermococcus kodakarensis AMP + ?
-
 ?
2.7.1.146 ADP + D-fructose 1,6-bisphosphate
-
 Thermococcus kodakarensis ATP + D-fructose 6-phosphate
-
 r
2.7.1.146 ADP + D-fructose 6-phosphate
-
 Thermococcus kodakarensis AMP + D-fructose 1,6-bisphosphate
-
 r
2.7.1.146 ADP + D-fructose 6-phosphate the rate of dephosphorylation of fructose 1,6-bisphosphate is 3times lower at 50°C than the phosphorylation of fructose 6-phosphate Thermococcus kodakarensis AMP + D-fructose 1,6-bisphosphate
-
 r
2.7.1.146 ADP + D-fructose 6-phosphate
-
 Thermococcus kodakarensis JCM 12380 AMP + D-fructose 1,6-bisphosphate
-
 r
2.7.1.146 ADP + D-fructose 6-phosphate
-
 Thermococcus kodakarensis ATCC BAA-918 AMP + D-fructose 1,6-bisphosphate
-
 r
2.7.1.146 ADP + D-glucose
-
 Thermococcus kodakarensis AMP + D-glucose 6-phosphate
-
 ?
2.7.1.146 ADP + D-glucose cf. EC 2.7.1.147 Thermococcus kodakarensis AMP + D-glucose 6-phosphate
-
 r
2.7.1.146 ADP + ribose 5-phosphate
-
 Thermococcus kodakarensis AMP + ?
-
 ?
2.7.1.146 ADP + ribose 5-phosphate
-
 Thermococcus kodakarensis AMP + ribose 1,5-bisphosphate
-
 r
2.7.1.146 ADP + thymidine
-
 Thermococcus kodakarensis AMP + ?
-
 ?
2.7.1.146 ADP + thymidine
-
 Thermococcus kodakarensis AMP + TMP
-
 r
2.7.1.146 AMP + D-fructose 1,6-bisphosphate
-
 Thermococcus kodakarensis ADP + D-fructose 6-phosphate
-
 r
2.7.1.146 ATP + D-fructose 6-phosphate
-
 Thermococcus kodakarensis ADP + D-fructose 1,6-bisphosphate
-
 r
2.7.1.146 ATP + D-fructose 6-phosphate 20% phosphofructokinase activity is observed in the presence of 2 mM ATP compared to 100% in the presence of equimolar ADP Thermococcus kodakarensis ADP + D-fructose 1,6-bisphosphate
-
 r
2.7.1.146 ATP + D-fructose 6-phosphate only 20% phosphofructokinase activity is observed in the presence of 2 mM ATP compared to 100% in the presence of equimolar ADP. No significant activity is detected in the presence of other phosphoryl donors examined Thermococcus kodakarensis ADP + D-fructose 1,6-bisphosphate
-
 r
2.7.1.146 ATP + D-fructose 6-phosphate
-
 Thermococcus kodakarensis JCM 12380 ADP + D-fructose 1,6-bisphosphate
-
 r
2.7.1.146 ATP + D-fructose 6-phosphate 20% phosphofructokinase activity is observed in the presence of 2 mM ATP compared to 100% in the presence of equimolar ADP Thermococcus kodakarensis JCM 12380 ADP + D-fructose 1,6-bisphosphate
-
 r
2.7.1.146 ATP + D-fructose 6-phosphate only 20% phosphofructokinase activity is observed in the presence of 2 mM ATP compared to 100% in the presence of equimolar ADP. No significant activity is detected in the presence of other phosphoryl donors examined Thermococcus kodakarensis JCM 12380 ADP + D-fructose 1,6-bisphosphate
-
 r
2.7.1.146 ATP + D-fructose 6-phosphate
-
 Thermococcus kodakarensis ATCC BAA-918 ADP + D-fructose 1,6-bisphosphate
-
 r
2.7.1.146 ATP + D-fructose 6-phosphate 20% phosphofructokinase activity is observed in the presence of 2 mM ATP compared to 100% in the presence of equimolar ADP Thermococcus kodakarensis ATCC BAA-918 ADP + D-fructose 1,6-bisphosphate
-
 r
2.7.1.146 ATP + D-fructose 6-phosphate only 20% phosphofructokinase activity is observed in the presence of 2 mM ATP compared to 100% in the presence of equimolar ADP. No significant activity is detected in the presence of other phosphoryl donors examined Thermococcus kodakarensis ATCC BAA-918 ADP + D-fructose 1,6-bisphosphate
-
 r
2.7.1.146 additional information the enzyme prefers ADP as phosphoryl donor, but ADP can be replaced by ATP resulting in a 5fold lower activity. The enzyme catalyzes the phosphorylation of fructose 6-phosphate and dephosphorylation of fructose 1,6-bisphosphate. In addition, it is able to phosphorylate D-glucose and nucleosides but with a much lower rate compared to that of fructose 6-phosphate, the enzyme shows 450fold lower activity with D-glucose (cf. EC 2.7.1.147) compared to that with fructose 6-phosphate. Only 20% phosphofructokinase activity is observed in the presence of 2 mM ATP compared to 100% in the presence of equimolar ADP. No significant activity is detected in the presence of other phosphoryl donors examined. For the phosphoryl acceptor specificity, a number of alternative substrates including nucleosides, sugars and sugar phosphates are examined. Among the nucleoside substrates, adenosine shows 12%, 2-deoxyadenosine 17.5%, and thymidine 18% consumption of ADP. Among sugars and sugar phosphates, 22% and 5% relative activities can be observed with glucose and ribose 5-phosphate, respectively. Substrate specificity, overview Thermococcus kodakarensis ?
-
-
2.7.1.146 additional information the enzyme prefers ADP as phosphoryl donor, but ADP can be replaced by ATP resulting in a 5fold lower activity. The enzyme catalyzes the phosphorylation of fructose 6-phosphate and dephosphorylation of fructose 1,6-bisphosphate. In addition, it is able to phosphorylate D-glucose and nucleosides but with a much lower rate compared to that of fructose 6-phosphate, the enzyme shows 450fold lower activity with D-glucose (cf. EC 2.7.1.147) compared to that with fructose 6-phosphate. Only 20% phosphofructokinase activity is observed in the presence of 2 mM ATP compared to 100% in the presence of equimolar ADP. No significant activity is detected in the presence of other phosphoryl donors examined. For the phosphoryl acceptor specificity, a number of alternative substrates including nucleosides, sugars and sugar phosphates are examined. Among the nucleoside substrates, adenosine shows 12%, 2-deoxyadenosine 17.5%, and thymidine 18% consumption of ADP. Among sugars and sugar phosphates, 22% and 5% relative activities can be observed with glucose and ribose 5-phosphate, respectively. Substrate specificity, overview Thermococcus kodakarensis JCM 12380 ?
-
-
2.7.1.146 additional information the enzyme prefers ADP as phosphoryl donor, but ADP can be replaced by ATP resulting in a 5fold lower activity. The enzyme catalyzes the phosphorylation of fructose 6-phosphate and dephosphorylation of fructose 1,6-bisphosphate. In addition, it is able to phosphorylate D-glucose and nucleosides but with a much lower rate compared to that of fructose 6-phosphate, the enzyme shows 450fold lower activity with D-glucose (cf. EC 2.7.1.147) compared to that with fructose 6-phosphate. Only 20% phosphofructokinase activity is observed in the presence of 2 mM ATP compared to 100% in the presence of equimolar ADP. No significant activity is detected in the presence of other phosphoryl donors examined. For the phosphoryl acceptor specificity, a number of alternative substrates including nucleosides, sugars and sugar phosphates are examined. Among the nucleoside substrates, adenosine shows 12%, 2-deoxyadenosine 17.5%, and thymidine 18% consumption of ADP. Among sugars and sugar phosphates, 22% and 5% relative activities can be observed with glucose and ribose 5-phosphate, respectively. Substrate specificity, overview Thermococcus kodakarensis ATCC BAA-918 ?
-
-

Subunits

EC Number Subunits Comment Organism
2.7.1.11 ? x * 52000, SDS-PAGE Thermococcus kodakarensis
2.7.1.146 ? x * 52000, SDS-PAGE Thermococcus kodakarensis
2.7.1.146 ? x * 52661, sequence calculation, x * 52000, recombinant enzyme, SDS-PAGE Thermococcus kodakarensis

Synonyms

EC Number Synonyms Comment Organism
2.7.1.11 ADP-dependent phosphofructokinase
-
 Thermococcus kodakarensis
2.7.1.146 ADP-dependent phosphofructokinase
-
 Thermococcus kodakarensis
2.7.1.146 PFK
-
 Thermococcus kodakarensis
2.7.1.146 pfkC
-
 Thermococcus kodakarensis
2.7.1.146 TK0376 locus name Thermococcus kodakarensis

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.7.1.11 90
-
-
 Thermococcus kodakarensis
2.7.1.146 90
-
-
 Thermococcus kodakarensis

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
2.7.1.11 70 100 70°C: about 45% of maximal activity, 100°C: about 75% of maximal activity Thermococcus kodakarensis
2.7.1.146 50 100 activity range, profile overview Thermococcus kodakarensis
2.7.1.146 70 100 70°C: about 45% of maximal activity, 100°C: about 75% of maximal activity Thermococcus kodakarensis

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
2.7.1.11 80
-
 no significant difference activity after incubation of 180 min Thermococcus kodakarensis
2.7.1.11 90
-
 half-life: 120 min Thermococcus kodakarensis
2.7.1.11 100
-
 half-life: 30 min Thermococcus kodakarensis
2.7.1.146 80
-
 no significant difference activity after incubation of 180 min Thermococcus kodakarensis
2.7.1.146 80
-
 purified enzyme, 180 min, completely stable Thermococcus kodakarensis
2.7.1.146 90
-
 half-life: 120 min Thermococcus kodakarensis
2.7.1.146 90
-
 purified enzyme, half life is 120 min Thermococcus kodakarensis
2.7.1.146 100
-
 half-life: 30 min Thermococcus kodakarensis
2.7.1.146 100
-
 purified enzyme, half life is 30 min Thermococcus kodakarensis

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2.7.1.11 95
-
 D-fructose 1,6-bisphosphate pH 7.5, 50°C, 5 mM Mg2+ Thermococcus kodakarensis
2.7.1.11 104
-
 D-glucose pH 7.5, 50°C, 5 mM Mg2+ Thermococcus kodakarensis
2.7.1.11 112
-
 AMP pH 7.5, 50°C, 5 mM Mg2+ Thermococcus kodakarensis
2.7.1.11 282
-
 D-fructose 6-phosphate pH 7.5, 50°C, 5 mM Mg2+ Thermococcus kodakarensis
2.7.1.11 338
-
 ADP pH 7.5, 50°C, 5 mM Mg2+ Thermococcus kodakarensis
2.7.1.11 475
-
 ADP pH 7.5, 50°C, 5 mM Co2+ Thermococcus kodakarensis
2.7.1.11 495
-
 D-fructose 6-phosphate pH 7.5, 50°C, 5 mM Co2+ Thermococcus kodakarensis
2.7.1.11 4328
-
 D-fructose 6-phosphate pH 7.5, 90°C, 5 mM Co2+ Thermococcus kodakarensis
2.7.1.11 4410
-
 ADP pH 7.5, 90°C, 5 mM Co2+ Thermococcus kodakarensis
2.7.1.146 95
-
 D-fructose 1,6-bisphosphate pH 7.5, 50°C, 5 mM Mg2+ Thermococcus kodakarensis
2.7.1.146 104
-
 D-glucose pH 7.5, 50°C, 5 mM Mg2+ Thermococcus kodakarensis
2.7.1.146 104
-
 D-glucose pH 7.5, 50°C, recombinant enzyme, in presence of Mg2+, substrates ADP and D-glucose Thermococcus kodakarensis
2.7.1.146 112
-
 AMP pH 7.5, 50°C, 5 mM Mg2+ Thermococcus kodakarensis
2.7.1.146 282
-
 D-fructose 6-phosphate pH 7.5, 50°C, 5 mM Mg2+ Thermococcus kodakarensis
2.7.1.146 282
-
 D-fructose 6-phosphate pH 7.5, 50°C, recombinant enzyme, in presence of Mg2+, substrates ADP and D-fructose 6-phosphate Thermococcus kodakarensis
2.7.1.146 338
-
 ADP pH 7.5, 50°C, 5 mM Mg2+ Thermococcus kodakarensis
2.7.1.146 338
-
 ADP pH 7.5, 50°C, recombinant enzyme, in presence of Mg2+, substrates ADP and D-fructose 6-phosphate Thermococcus kodakarensis
2.7.1.146 475
-
 ADP pH 7.5, 50°C, 5 mM Co2+ Thermococcus kodakarensis
2.7.1.146 475
-
 ADP pH 7.5, 50°C, recombinant enzyme, in presence of Co2+, substrates ADP and D-fructose 6-phosphate Thermococcus kodakarensis
2.7.1.146 495
-
 D-fructose 6-phosphate pH 7.5, 50°C, 5 mM Co2+ Thermococcus kodakarensis
2.7.1.146 495
-
 D-fructose 6-phosphate pH 7.5, 50°C, recombinant enzyme, in presence of Co2+, substrates ADP and D-fructose 6-phosphate Thermococcus kodakarensis
2.7.1.146 4238
-
 D-fructose 6-phosphate pH 7.5, 90°C, recombinant enzyme, in presence of Co2+, substrates ADP and D-fructose 6-phosphate Thermococcus kodakarensis
2.7.1.146 4328
-
 D-fructose 6-phosphate pH 7.5, 90°C, 5 mM Co2+ Thermococcus kodakarensis
2.7.1.146 4410
-
 ADP pH 7.5, 90°C, 5 mM Co2+ Thermococcus kodakarensis
2.7.1.146 4410
-
 ADP pH 7.5, 90°C, recombinant enzyme, in presence of Co2+, substrates ADP and D-fructose 6-phosphate Thermococcus kodakarensis

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.7.1.11 7.4
-
-
 Thermococcus kodakarensis
2.7.1.146 7.4
-
-
 Thermococcus kodakarensis
2.7.1.146 7.5
-
 assay at Thermococcus kodakarensis

pH Range

EC Number pH Minimum pH Maximum Comment Organism
2.7.1.11 6 8 pH 6.0: about 80% of maximal activity, pH 8.0: about 45% of maximal activity Thermococcus kodakarensis
2.7.1.146 4 9 activity range, profile overview Thermococcus kodakarensis
2.7.1.146 6 8 pH 6.0: about 80% of maximal activity, pH 8.0: about 45% of maximal activity Thermococcus kodakarensis

Cofactor

EC Number Cofactor Comment Organism Structure
2.7.1.146 ADP
-
 Thermococcus kodakarensis

pI Value

EC Number Organism Comment pI Value Maximum pI Value
2.7.1.146 Thermococcus kodakarensis sequence calculation
-
 6.71

General Information

EC Number General Information Comment Organism
2.7.1.146 physiological function the phosphofructokinase activity of the enzyme is not allosterically regulated Thermococcus kodakarensis

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
2.7.1.11 115
-
 D-glucose pH 7.5, 50°C, 5 mM Mg2+ Thermococcus kodakarensis
2.7.1.11 127
-
 D-fructose 1,6-bisphosphate pH 7.5, 50°C, 5 mM Mg2+ Thermococcus kodakarensis
2.7.1.11 138
-
 ADP pH 7.5, 90°C, 5 mM Co2+ Thermococcus kodakarensis
2.7.1.11 457
-
 ADP pH 7.5, 50°C, 5 mM Mg2+ Thermococcus kodakarensis
2.7.1.11 513
-
 D-fructose 6-phosphate pH 7.5, 50°C, 5 mM Mg2+ Thermococcus kodakarensis
2.7.1.11 552
-
 ADP pH 7.5, 50°C, 5 mM Co2+ Thermococcus kodakarensis
2.7.1.11 565
-
 D-fructose 6-phosphate pH 7.5, 90°C, 5 mM Co2+ Thermococcus kodakarensis
2.7.1.11 713
-
 AMP pH 7.5, 50°C, 5 mM Mg2+ Thermococcus kodakarensis
2.7.1.11 851
-
 D-fructose 6-phosphate pH 7.5, 50°C, 5 mM Co2+ Thermococcus kodakarensis
2.7.1.146 1.16
-
 D-glucose pH 7.5, 50°C, recombinant enzyme, in presence of Mg2+, substrates ADP and D-glucose Thermococcus kodakarensis
2.7.1.146 115
-
 D-glucose pH 7.5, 50°C, 5 mM Mg2+ Thermococcus kodakarensis
2.7.1.146 127
-
 D-fructose 1,6-bisphosphate pH 7.5, 50°C, 5 mM Mg2+ Thermococcus kodakarensis
2.7.1.146 138
-
 ADP pH 7.5, 90°C, 5 mM Co2+ Thermococcus kodakarensis
2.7.1.146 456.8
-
 ADP pH 7.5, 50°C, recombinant enzyme, in presence of Mg2+, substrates ADP and D-fructose 6-phosphate Thermococcus kodakarensis
2.7.1.146 457
-
 ADP pH 7.5, 50°C, 5 mM Mg2+ Thermococcus kodakarensis
2.7.1.146 512.7
-
 D-fructose 6-phosphate pH 7.5, 50°C, recombinant enzyme, in presence of Mg2+, substrates ADP and D-fructose 6-phosphate Thermococcus kodakarensis
2.7.1.146 513
-
 D-fructose 6-phosphate pH 7.5, 50°C, 5 mM Mg2+ Thermococcus kodakarensis
2.7.1.146 552
-
 ADP pH 7.5, 50°C, 5 mM Co2+ Thermococcus kodakarensis
2.7.1.146 552
-
 ADP pH 7.5, 50°C, recombinant enzyme, in presence of Co2+, substrates ADP and D-fructose 6-phosphate Thermococcus kodakarensis
2.7.1.146 565
-
 D-fructose 6-phosphate pH 7.5, 90°C, 5 mM Co2+ Thermococcus kodakarensis
2.7.1.146 713
-
 AMP pH 7.5, 50°C, 5 mM Mg2+ Thermococcus kodakarensis
2.7.1.146 851
-
 D-fructose 6-phosphate pH 7.5, 50°C, 5 mM Co2+ Thermococcus kodakarensis
2.7.1.146 853.5
-
 D-fructose 6-phosphate pH 7.5, 50°C, recombinant enzyme, in presence of Co2+, substrates ADP and D-fructose 6-phosphate Thermococcus kodakarensis
2.7.1.146 5727
-
 D-fructose 6-phosphate pH 7.5, 90°C, recombinant enzyme, in presence of Co2+, substrates ADP and D-fructose 6-phosphate Thermococcus kodakarensis
2.7.1.146 13781
-
 ADP pH 7.5, 90°C, recombinant enzyme, in presence of Co2+, substrates ADP and D-fructose 6-phosphate Thermococcus kodakarensis