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Literature summary extracted from

  • Satomura, T.; Hayashi, J.; Sakamoto, H.; Nunoura, T.; Takaki, Y.; Takai, K.; Takami, H.; Ohshima, T.; Sakuraba, H.; Suye, S.I.
    D-Lactate electrochemical biosensor prepared by immobilization of thermostabledye-linked D-lactate dehydrogenase from Candidatus Caldiarchaeum subterraneum (2018), J. Biosci. Bioeng., 126, 425-430 .
    View publication on PubMed

Application

EC Number Application Comment Organism
1.1.3.15 analysis construction of a D-Lactate electrochemical biosensor. The electrode for detection of D-lactate is prepared by immobilizing dye-linked D-LDH and multi-walled carbon nanotube within Nafion membrane. The electrode response to D-lactate is linear within the concentration range of 0.03-2.5 mM, and it shows little reduction in responsiveness after 50 days Candidatus Caldarchaeum subterraneum
1.1.99.6 analysis construction of a D-Lactate electrochemical biosensor. The electrode for detection of D-lactate is prepared by immobilizing dye-linked D-LDH and multi-walled carbon nanotube within Nafion membrane. The electrode response to D-lactate is linear within the concentration range of 0.03-2.5 mM, and it shows little reduction in responsiveness after 50 days Candidatus Caldarchaeum subterraneum
1.1.99.6 analysis D-lactate content is of great interest for food analysis. A D-lactate sensing system using a thermostable dye-linked D-lactate dehydrogenase (Dye-DLDH) is developed. The electrode for detection of D-lactate is prepared by immobilizing the thermostable Dye-DLDH and multi-walled carbon nanotube (MWCNT) within Nafion membrane. The electrocatalytic response of the electrode is clearly observed upon exposure to D-lactate. The electrode response to D-lactate is linear within the concentration range of 0.03-2.5 mM, and it shows little reduction in responsiveness after 50 days Candidatus Caldarchaeum subterraneum
1.1.99.6 diagnostics D-lactate content is of great interest for clinical diagnosis. A D-lactate sensing system using a thermostable dye-linked D-lactate dehydrogenase (Dye-DLDH) is developed. The electrode for detection of D-lactate is prepared by immobilizing the thermostable Dye-DLDH and multi-walled carbon nanotube (MWCNT) within Nafion membrane. The electrocatalytic response of the electrode is clearly observed upon exposure to D-lactate. The electrode response to D-lactate is linear within the concentration range of 0.03-2.5 mM, and it shows little reduction in responsiveness after 50 days Candidatus Caldarchaeum subterraneum

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.1.3.15 expression in Escherichia coli Candidatus Caldarchaeum subterraneum
1.1.99.6 expression in Escherichia coli Candidatus Caldarchaeum subterraneum
1.1.99.6 overexpressed in Escherichia coli Candidatus Caldarchaeum subterraneum

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.1.99.6 0.605
-
 (R)-lactate pH 7.0, 60°C, electron acceptor: oxidized 2,6-dichlorophenolindophenol Candidatus Caldarchaeum subterraneum

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.1.3.15 114000
-
 gel filtration Candidatus Caldarchaeum subterraneum
1.1.99.6 114000
-
 gel filtration Candidatus Caldarchaeum subterraneum

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.1.99.6 (R)-lactate + acceptor Candidatus Caldarchaeum subterraneum
-
 pyruvate + reduced acceptor
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.1.3.15 Candidatus Caldarchaeum subterraneum E6N788
-
-
1.1.99.6 Candidatus Caldarchaeum subterraneum E6N788
-
-
1.1.99.6 Candidatus Caldarchaeum subterraneum E6N788 cf. EC 1.1.3.15
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.1.99.6
-
 Candidatus Caldarchaeum subterraneum

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.1.99.6 3.18
-
 pH 7.0, 60°C Candidatus Caldarchaeum subterraneum

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.3.15 (R)-2-hydroxybutanoate + 2,6-dichlorophenolindophenol preferred substrate Candidatus Caldarchaeum subterraneum 2-oxobutanoate + reduced 2,6-dichlorophenolindophenol
-
 ?
1.1.3.15 (R)-lactate + 2,6-dichlorophenolindophenol preferred substrate Candidatus Caldarchaeum subterraneum pyruvate + reduced 2,6-dichlorophenolindophenol
-
 ?
1.1.3.15 additional information no substrates: D-alanine, pyruvate, D-glucose, L-alanine, ethanol and L-lactate Candidatus Caldarchaeum subterraneum ?
-
-
1.1.99.6 (R)-2-hydroxybutanoate + 2,6-dichlorophenolindophenol preferred substrate Candidatus Caldarchaeum subterraneum 2-oxobutanoate + reduced 2,6-dichlorophenolindophenol
-
 ?
1.1.99.6 (R)-2-hydroxybutyrate + oxidized 2,6-dichlorophenolindophenol relative activity is 46% compared to D-lactate Candidatus Caldarchaeum subterraneum 2-oxobutyrate + reduced 2,6-dichlorophenolindophenol
-
 ?
1.1.99.6 (R)-lactate + 2,6-dichlorophenolindophenol preferred substrate Candidatus Caldarchaeum subterraneum pyruvate + reduced 2,6-dichlorophenolindophenol
-
 ?
1.1.99.6 (R)-lactate + acceptor
-
 Candidatus Caldarchaeum subterraneum pyruvate + reduced acceptor
-
 ?
1.1.99.6 (R)-lactate + ferricyanide
-
 Candidatus Caldarchaeum subterraneum pyruvate + ferrocyanide + H+
-
 ?
1.1.99.6 (R)-lactate + oxidized 2,6-dichlorophenolindophenol (R)-lactate is the most preferred substrate, followed by (R)-2-hydroxybutyrate. D-Alanine, pyruvate, D-glucose, L-alanine, ethanol and L-lactate are inert as electron donors. Benzyl viologen and methylene blue are inert as electron acceptors Candidatus Caldarchaeum subterraneum pyruvate + reduced 2,6-dichlorophenolindophenol
-
 ?
1.1.99.6 (R)-lactate + oxidized 3-[4,5-dimethylthiazol-2-yl]-2,5-diphenyltetrazolium bromide
-
 Candidatus Caldarchaeum subterraneum pyruvate + reduced 3-[4,5-dimethylthiazol-2-yl]-2,5-diphenyltetrazolium bromide
-
 ?
1.1.99.6 additional information no substrates: D-alanine, pyruvate, D-glucose, L-alanine, ethanol and L-lactate Candidatus Caldarchaeum subterraneum ?
-
-

Subunits

EC Number Subunits Comment Organism
1.1.3.15 dimer 2 * 51000, SDS-PAGE Candidatus Caldarchaeum subterraneum
1.1.99.6 dimer 2 * 51000, SDS-PAGE Candidatus Caldarchaeum subterraneum
1.1.99.6 homodimer 2 * 51000, SDS-PAGE Candidatus Caldarchaeum subterraneum

Synonyms

EC Number Synonyms Comment Organism
1.1.3.15 CSUB_C1080
-
 Candidatus Caldarchaeum subterraneum
1.1.99.6 CSUB_C1080
-
 Candidatus Caldarchaeum subterraneum
1.1.99.6 dye-DLDH
-
 Candidatus Caldarchaeum subterraneum
1.1.99.6 dye-linked D-lactate dehydrogenase
-
 Candidatus Caldarchaeum subterraneum

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
1.1.3.15 50
-
 30 min, pH 6.0 or pH 8.0, no loss of activity Candidatus Caldarchaeum subterraneum
1.1.3.15 70
-
 10 min, no loss of acitvity Candidatus Caldarchaeum subterraneum
1.1.99.6 50
-
 30 min, pH 6.0 or pH 8.0, no loss of activity Candidatus Caldarchaeum subterraneum
1.1.99.6 70
-
 10 min, no loss of acitvity Candidatus Caldarchaeum subterraneum
1.1.99.6 70
-
 retains full activity after incubation for 10 min at 70°C Candidatus Caldarchaeum subterraneum
1.1.99.6 80
-
 10 min, 30% loss of activity Candidatus Caldarchaeum subterraneum
1.1.99.6 90
-
 10 min, 90% loss of activity Candidatus Caldarchaeum subterraneum

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.1.3.15 9
-
-
 Candidatus Caldarchaeum subterraneum
1.1.99.6 9
-
-
 Candidatus Caldarchaeum subterraneum

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
1.1.3.15 5
-
 30 min, 50°C, 40% loss of activity Candidatus Caldarchaeum subterraneum
1.1.3.15 6
-
 30 min, 50°C, no loss of activity Candidatus Caldarchaeum subterraneum
1.1.3.15 8
-
 30 min, 50°C, no loss of activity Candidatus Caldarchaeum subterraneum
1.1.3.15 9
-
 30 min, 50°C, 40% loss of activity Candidatus Caldarchaeum subterraneum
1.1.99.6 5
-
 30 min, 50°C, 40% loss of activity Candidatus Caldarchaeum subterraneum
1.1.99.6 6
-
 30 min, 50°C, no loss of activity Candidatus Caldarchaeum subterraneum
1.1.99.6 8
-
 30 min, 50°C, no loss of activity Candidatus Caldarchaeum subterraneum
1.1.99.6 9
-
 30 min, 50°C, 40% loss of activity Candidatus Caldarchaeum subterraneum

Cofactor

EC Number Cofactor Comment Organism Structure
1.1.3.15 FAD
-
 Candidatus Caldarchaeum subterraneum
1.1.99.6 FAD
-
 Candidatus Caldarchaeum subterraneum
1.1.99.6 FAD cofactor Candidatus Caldarchaeum subterraneum