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Literature summary extracted from

  • Cho, H.J.; Cho, H.Y.; Park, J.W.; Kwon, O.S.; Lee, H.S.; Huh, T.L.; Kang, B.S.
    NADP+-dependent cytosolic isocitrate dehydrogenase provides NADPH in the presence of cadmium due to the moderate chelating effect of glutathione (2018), J. Biol. Inorg. Chem., 23, 849-860 .
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.1.1.42 expressed in Escherichia coli BL21(DE3) cells Mus musculus

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
1.1.1.42 hanging drop vapor diffusion method, using 0.1 M sodium citrate, pH 5.5, 16% (w/v) PEG 4000, 20% (v/v) isopropanol Mus musculus

Protein Variants

EC Number Protein Variants Comment Organism
1.1.1.42 C245S the activities of the mutant in the presence of Cd2+ are decreased as that of wild type enzyme Mus musculus
1.1.1.42 C379S the activity of the mutant in the absence of Cd2+ is similar to that of the wild type enzyme, but the decrease of activity in the presence of Cd2+ is much reduced Mus musculus

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.1.1.42 Cd2+ above 0.1 mM, the enzyme loses activity, and at 2 mM Cd2+ most of the activity has disappeared. Chelation of Cd2+ by dithiothreitol cannot recover the lost enzyme activity. Inactivation of the enzyme by Cd2+ is less effective when the enzyme is activated with Cd2+ than Mg2+, More than 50% of the activity of the enzyme activated with 0.05 mM Cd2+ remains in the presence of 1 mM GSH Mus musculus
1.1.1.42 Mn2+ the specific activity of purified enzyme is increased by Mn2+ in the micromolar range Mus musculus

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
1.1.1.42 cytosol
-
Mus musculus 5829
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.1.1.42 Cd2+ the enzyme activity is increased at a Cd2+ concentration below 0.1 mM Mus musculus
1.1.1.42 Mg2+ the maximum activity with Mg2+ is slightly lower than that with Cd2+ Mus musculus
1.1.1.42 additional information Ca2+ does not activate Mg2+ Mus musculus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.1.1.42 isocitrate + NADP+ Mus musculus
-
2-oxoglutarate + CO2 + NADPH + H+
-
?

Organism

EC Number Organism UniProt Comment Textmining
1.1.1.42 Mus musculus O88844
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.1.1.42 glutathione Sepharose column chromatography Mus musculus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.1.42 isocitrate + NADP+
-
Mus musculus 2-oxoglutarate + CO2 + NADPH + H+
-
?

Synonyms

EC Number Synonyms Comment Organism
1.1.1.42 IDH
-
Mus musculus
1.1.1.42 NADP+-dependent isocitrate dehydrogenase
-
Mus musculus

Cofactor

EC Number Cofactor Comment Organism Structure
1.1.1.42 NADP+
-
Mus musculus