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Literature summary extracted from

  • Yadav, P.; Vitvitsky, V.; Carballal, S.; Seravalli, J.; Banerjee, R.
    Thioredoxin regulates human mercaptopyruvate sulfurtransferase at physiologically-relevant concentrations (2020), J. Biol. Chem., 295, 6299-6310 .
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.8.1.2 expression in Escherichia coli Mus musculus
2.8.1.2 expression in Escherichia coli Homo sapiens

Protein Variants

EC Number Protein Variants Comment Organism
2.8.1.2 D63A mitochondrial splice variant MPST2, mutation of catalytic triad, mutation of catalytic triad, specific activity in presence of cysteine is comparable to wild-type, specific activity with thioredoxin is 2.3fold decreased Mus musculus
2.8.1.2 D63A mitochondrial splice variant MPST2, mutation of catalytic triad, mutation of catalytic triad, specific activity in presence of cysteine is comparable to wild-type, specific activity with thioredoxin is 2.3fold decreased Homo sapiens
2.8.1.2 H74A mitochondrial splice variant MPST2, mutation of catalytic triad, specific activity in presence of cysteine is comparable to wild-type, specific activity with thioredoxin is 1.9fold decreased Mus musculus
2.8.1.2 H74A mitochondrial splice variant MPST2, mutation of catalytic triad, specific activity in presence of cysteine is comparable to wild-type, specific activity with thioredoxin is 1.9fold decreased Homo sapiens
2.8.1.2 S250A mitochondrial splice variant MPST2, mutation of catalytic triad, specific activity with cysteine is 2.6fold lower than wild-type, with thioredoxin, specific activity is similar to wild-type Mus musculus
2.8.1.2 S250A mitochondrial splice variant MPST2, mutation of catalytic triad, specific activity with cysteine is 2.6fold lower than wild-type, with thioredoxin, specific activity is similar to wild-type Homo sapiens

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.8.1.2 thioredoxin substrate inhibition of mitochondrial splice variant MPST2 Homo sapiens
2.8.1.2 thioredoxin substrate inhibition of mitochondrial splice variant MPST2 Mus musculus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.8.1.2 0.003
-
 thioredoxin cytosolic splice variant MPST1, Hill coefficient 1.6, pH 7.4, 37°C Homo sapiens
2.8.1.2 0.0031
-
 thioredoxin mitochondrial splice variant MPST2, Hill coefficient 1.4, pH 7.4, 37°C Homo sapiens
2.8.1.2 0.015
-
 3-Mercaptopyruvate cosubstrate N-acetyl-L-cysteine, mitochondrial splice variant MPST2, Hill coefficient 1.1, pH 7.4, 37°C Homo sapiens
2.8.1.2 0.02
-
 3-Mercaptopyruvate cosubstrate N-acetyl-L-cysteine, cytosolic splice variant MPST1, Hill coefficient 1.3, pH 7.4, 37°C Homo sapiens
2.8.1.2 0.022
-
 3-Mercaptopyruvate cosubstrate L-cysteine, mitochondrial splice variant MPST2, Hill coefficient 1.4, pH 7.4, 37°C Homo sapiens
2.8.1.2 0.024
-
 3-Mercaptopyruvate cosubstrate L-cysteine, cytosolic splice variant MPST1, Hill coefficient 1.6, pH 7.4, 37°C Homo sapiens
2.8.1.2 0.358
-
 3-Mercaptopyruvate cosubstrate thioredoxin, cytosolic splice variant MPST1, Hill coefficient 2.0, pH 7.4, 37°C Homo sapiens
2.8.1.2 0.36
-
 3-Mercaptopyruvate cosubstrate thioredoxin, mitochondrial splice variant MPST2, Hill coefficient 2.1, pH 7.4, 37°C Homo sapiens
2.8.1.2 3.8
-
 L-cysteine cytosolic splice variant MPST1, Hill coefficient 1.4, pH 7.4, 37°C Homo sapiens
2.8.1.2 4.5
-
 L-cysteine mitochondrial splice variant MPST2, Hill coefficient 1.6, pH 7.4, 37°C Homo sapiens
2.8.1.2 16
-
 N-acetyl-L-cysteine mitochondrial splice variant MPST2, Hill coefficient 2.7, pH 7.4, 37°C Homo sapiens
2.8.1.2 17
-
 N-acetyl-L-cysteine cytosolic splice variant MPST1, Hill coefficient 2.2, pH 7.4, 37°C Homo sapiens

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.8.1.2 cytosol two splice variants of MPST, differing by 20 amino acids at the N-terminus, give rise to the cytosolic MPST1 and mitochondrial MPST2 isoforms Homo sapiens 5829
-
2.8.1.2 mitochondrion two splice variants of MPST, differing by 20 amino acids at the N-terminus, give rise to the cytosolic MPST1 and mitochondrial MPST2 isoforms Homo sapiens 5739
-

Organism

EC Number Organism UniProt Comment Textmining
2.8.1.2 Homo sapiens P25325
-
-
2.8.1.2 Mus musculus Q99J99
-
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.8.1.2 brain 10fold lower activity than in liver Mus musculus
-
2.8.1.2 kidney 2fold lower activity than in liver Mus musculus
-
2.8.1.2 liver highest activity observed Mus musculus
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.8.1.2 0.7
-
 mitochondrial splice variant MPST2, mutant S250A, substrate L-cysteine, pH 7.4, 37°C Homo sapiens
2.8.1.2 1
-
 mitochondrial splice variant MPST2, mutant D63A, substrate thioredoxin, pH 7.4, 37°C Homo sapiens
2.8.1.2 1.2
-
 mitochondrial splice variant MPST2, mutant H74A, substrate thioredoxin, pH 7.4, 37°C Homo sapiens
2.8.1.2 1.4
-
 mitochondrial splice variant MPST2, mutant D63A, substrate L-cysteine, pH 7.4, 37°C Homo sapiens
2.8.1.2 1.6
-
 mitochondrial splice variant MPST2, mutant H74A, substrate L-cysteine, pH 7.4, 37°C Homo sapiens
2.8.1.2 1.8
-
 wild-type mitochondrial splice variant MPST2, substrate L-cysteine, pH 7.4, 37°C Homo sapiens
2.8.1.2 2.3
-
 mitochondrial splice variant MPST2, mutant S250A, substrate thioredoxin, pH 7.4, 37°C Homo sapiens
2.8.1.2 2.3
-
 wild-type mitochondrial splice variant MPST2, substrate thioredoxin, pH 7.4, 37°C Homo sapiens

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.8.1.2 3-mercaptopyruvate + L-cysteine
-
 Mus musculus ?
-
 ?
2.8.1.2 3-mercaptopyruvate + L-cysteine
-
 Homo sapiens ?
-
 ?
2.8.1.2 3-mercaptopyruvate + N-acetyl-L-cysteine
-
 Mus musculus ?
-
 ?
2.8.1.2 3-mercaptopyruvate + N-acetyl-L-cysteine
-
 Homo sapiens ?
-
 ?
2.8.1.2 3-mercaptopyruvate + thioredoxin
-
 Mus musculus pyruvate + persulfurated thioredoxin
-
 ?
2.8.1.2 3-mercaptopyruvate + thioredoxin
-
 Homo sapiens pyruvate + persulfurated thioredoxin
-
 ?

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2.8.1.2 0.22
-
 N-acetyl-L-cysteine cytosolic splice variant MPST1, Hill coefficient 2.2, pH 7.4, 37°C Homo sapiens
2.8.1.2 0.27
-
 N-acetyl-L-cysteine mitochondrial splice variant MPST2, Hill coefficient 2.2, pH 7.4, 37°C Homo sapiens
2.8.1.2 0.4
-
 thioredoxin cytosolic splice variant MPST1, Hill coefficient 1.6, pH 7.4, 37°C Homo sapiens
2.8.1.2 0.44
-
 L-cysteine cytosolic splice variant MPST1, Hill coefficient 1.4, pH 7.4, 37°C Homo sapiens
2.8.1.2 0.44
-
 thioredoxin mitochondrial splice variant MPST2, Hill coefficient 1.6, pH 7.4, 37°C Homo sapiens
2.8.1.2 0.5
-
 L-cysteine mitochondrial splice variant MPST2, Hill coefficient 1.4, pH 7.4, 37°C Homo sapiens

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
2.8.1.2 0.013
-
 N-acetyl-L-cysteine cytosolic splice variant MPST1, Hill coefficient 2., pH 7.4, 37°C Homo sapiens
2.8.1.2 0.017
-
 N-acetyl-L-cysteine mitochondrial splice variant MPST2, Hill coefficient 2.2, pH 7.4, 37°C Homo sapiens
2.8.1.2 0.11
-
 L-cysteine cytosolic splice variant MPST1, Hill coefficient 1.4, pH 7.4, 37°C Homo sapiens
2.8.1.2 0.11
-
 L-cysteine mitochondrial splice variant MPST2, Hill coefficient 1.4, pH 7.4, 37°C Homo sapiens
2.8.1.2 0.44
-
 thioredoxin mitochondrial splice variant MPST2, Hill coefficient 1.6, pH 7.4, 37°C Homo sapiens
2.8.1.2 133
-
 thioredoxin cytosolic splice variant MPST1, Hill coefficient 1.6, pH 7.4, 37°C Homo sapiens