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Literature summary extracted from

  • Koendjbiharie, J.G.; Hon, S.; Pabst, M.; Hooftman, R.; Stevenson, D.M.; Cui, J.; Amador-Noguez, D.; Lynd, L.R.; Olson, D.G.; van Kranenburg, R.
    The pentose phosphate pathway of cellulolytic clostridia relies on 6-phosphofructokinase instead of transaldolase (2020), J. Biol. Chem., 295, 1867-1878 .
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.7.1.11
-
 Escherichia coli
2.7.1.11 overexpression in Escherichia coli Clostridium thermosuccinogenes
2.7.1.90 overexpression in Escherichia coli Acetivibrio thermocellus
2.7.1.90 overexpression in Escherichia coli Clostridium thermosuccinogenes

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.7.1.11 0.065
-
 D-fructose 6-phosphate pH 7.0, 55°C Escherichia coli
2.7.1.11 0.688
-
 D-fructose 6-phosphate pH 7.0, 55°C Clostridium thermosuccinogenes
2.7.1.11 2.5
-
 sedoheptulose 7-phosphate pH 7.0, 55°C Escherichia coli
2.7.1.90 0.046
-
 D-fructose 6-phosphate pH 7.0, 55°C Acetivibrio thermocellus
2.7.1.90 0.07
-
 D-fructose 6-phosphate pH 7.0, 55°C Clostridium thermosuccinogenes
2.7.1.90 0.1
-
 sedoheptulose 7-phosphate pH 7.0, 55°C Acetivibrio thermocellus
2.7.1.90 0.11
-
 sedoheptulose 7-phosphate pH 7.0, 55°C Clostridium thermosuccinogenes

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.7.1.90 diphosphate + sedoheptulose 7-phosphate Acetivibrio thermocellus the pentose phosphate pathway of cellulolytic clostridia relies on pyrophosphate-dependent 6-phosphofructokinase instead of transaldolase phosphate + sedoheptulose 1,7-bisphosphate
-
 ?
2.7.1.90 diphosphate + sedoheptulose 7-phosphate Clostridium thermosuccinogenes the pentose phosphate pathway of cellulolytic clostridia relies on pyrophosphate-dependent 6-phosphofructokinase instead of transaldolase phosphate + sedoheptulose 1,7-bisphosphate
-
 ?
2.7.1.90 diphosphate + sedoheptulose 7-phosphate Acetivibrio thermocellus DSM 1313 the pentose phosphate pathway of cellulolytic clostridia relies on pyrophosphate-dependent 6-phosphofructokinase instead of transaldolase phosphate + sedoheptulose 1,7-bisphosphate
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.7.1.11 Clostridium thermosuccinogenes
-
-
-
2.7.1.11 Escherichia coli P0A796
-
-
2.7.1.11 Escherichia coli BW25113 P0A796
-
-
2.7.1.90 Acetivibrio thermocellus A0A2K2FKR3
-
-
2.7.1.90 Acetivibrio thermocellus DSM 1313 A0A2K2FKR3
-
-
2.7.1.90 Clostridium thermosuccinogenes PNT94038.1
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.7.1.11
-
 Clostridium thermosuccinogenes
2.7.1.11
-
 Escherichia coli
2.7.1.90
-
 Acetivibrio thermocellus
2.7.1.90
-
 Clostridium thermosuccinogenes

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.1.11 ATP + D-fructose 6-phosphate the enzyme shows an extremely high degree of cooperative binding, resulting in a virtual on/off switch for substrate concentrations near its K1/2 value Clostridium thermosuccinogenes ADP + D-fructose 1,6-bisphosphate
-
 ?
2.7.1.11 ATP + D-fructose 6-phosphate
-
 Escherichia coli ADP + D-fructose 1,6-bisphosphate
-
 ?
2.7.1.11 ATP + D-fructose 6-phosphate
-
 Escherichia coli BW25113 ADP + D-fructose 1,6-bisphosphate
-
 ?
2.7.1.11 ATP + sedoheptulose 7-phosphate
-
 Escherichia coli ADP + sedoheptulose 1,7-bisphosphate
-
 ?
2.7.1.11 ATP + sedoheptulose 7-phosphate
-
 Escherichia coli BW25113 ADP + sedoheptulose 1,7-bisphosphate
-
 ?
2.7.1.11 GTP + D-fructose 6-phosphate
-
 Clostridium thermosuccinogenes GDP + D-fructose 1,6-bisphosphate
-
 ?
2.7.1.90 diphosphate + D-fructose 6-phosphate the enzyme has similar affinity for D-fructose 6-phosphate and sedoheptulose 7-phosphate Acetivibrio thermocellus phosphate + D-fructose 1,6-bisphosphate
-
 ?
2.7.1.90 diphosphate + D-fructose 6-phosphate the enzyme has similar affinity for D-fructose 6-phosphate sedoheptulose 7-phosphate Clostridium thermosuccinogenes phosphate + D-fructose 1,6-bisphosphate
-
 ?
2.7.1.90 diphosphate + D-fructose 6-phosphate the enzyme has similar affinity for D-fructose 6-phosphate and sedoheptulose 7-phosphate Acetivibrio thermocellus DSM 1313 phosphate + D-fructose 1,6-bisphosphate
-
 ?
2.7.1.90 diphosphate + sedoheptulose 7-phosphate the pentose phosphate pathway of cellulolytic clostridia relies on pyrophosphate-dependent 6-phosphofructokinase instead of transaldolase Acetivibrio thermocellus phosphate + sedoheptulose 1,7-bisphosphate
-
 ?
2.7.1.90 diphosphate + sedoheptulose 7-phosphate the pentose phosphate pathway of cellulolytic clostridia relies on pyrophosphate-dependent 6-phosphofructokinase instead of transaldolase Clostridium thermosuccinogenes phosphate + sedoheptulose 1,7-bisphosphate
-
 ?
2.7.1.90 diphosphate + sedoheptulose 7-phosphate the enzyme has similar affinity for D-fructose 6-phosphate and sedoheptulose 7-phosphate Acetivibrio thermocellus phosphate + sedoheptulose 1,7-bisphosphate
-
 ?
2.7.1.90 diphosphate + sedoheptulose 7-phosphate the enzyme has similar affinity for D-fructose 6-phosphate sedoheptulose 7-phosphate Clostridium thermosuccinogenes phosphate + sedoheptulose 1,7-bisphosphate
-
 ?
2.7.1.90 diphosphate + sedoheptulose 7-phosphate the pentose phosphate pathway of cellulolytic clostridia relies on pyrophosphate-dependent 6-phosphofructokinase instead of transaldolase Acetivibrio thermocellus DSM 1313 phosphate + sedoheptulose 1,7-bisphosphate
-
 ?
2.7.1.90 diphosphate + sedoheptulose 7-phosphate the enzyme has similar affinity for D-fructose 6-phosphate and sedoheptulose 7-phosphate Acetivibrio thermocellus DSM 1313 phosphate + sedoheptulose 1,7-bisphosphate
-
 ?

Synonyms

EC Number Synonyms Comment Organism
2.7.1.11 A/GTP-PFK
-
 Clostridium thermosuccinogenes
2.7.1.11 PFKA
-
 Escherichia coli
2.7.1.90 PPi-PFK
-
 Acetivibrio thermocellus
2.7.1.90 PPi-PFK
-
 Clostridium thermosuccinogenes
2.7.1.90 pyrophosphate-dependent phosphofructokinase
-
 Acetivibrio thermocellus
2.7.1.90 pyrophosphate-dependent phosphofructokinase
-
 Clostridium thermosuccinogenes

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2.7.1.11 0.25
-
 sedoheptulose 7-phosphate pH 7.0, 55°C Escherichia coli
2.7.1.11 1.2
-
 D-fructose 6-phosphate pH 7.0, 55°C Escherichia coli
2.7.1.11 41
-
 D-fructose 6-phosphate pH 7.0, 55°C Clostridium thermosuccinogenes
2.7.1.90 49
-
 sedoheptulose 7-phosphate pH 7.0, 55°C Clostridium thermosuccinogenes
2.7.1.90 80
-
 sedoheptulose 7-phosphate pH 7.0, 55°C Acetivibrio thermocellus
2.7.1.90 127
-
 D-fructose 6-phosphate pH 7.0, 55°C Acetivibrio thermocellus
2.7.1.90 182
-
 D-fructose 6-phosphate pH 7.0, 55°C Clostridium thermosuccinogenes

General Information

EC Number General Information Comment Organism
2.7.1.90 metabolism the pentose phosphate pathway of cellulolytic clostridia relies on pyrophosphate-dependent 6-phosphofructokinase instead of transaldolase Acetivibrio thermocellus
2.7.1.90 metabolism the pentose phosphate pathway of cellulolytic clostridia relies on pyrophosphate-dependent 6-phosphofructokinase instead of transaldolase Clostridium thermosuccinogenes

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
2.7.1.11 0.15
-
 sedoheptulose 7-phosphate pH 7.0, 55°C Escherichia coli
2.7.1.11 19
-
 D-fructose 6-phosphate pH 7.0, 55°C Escherichia coli
2.7.1.11 59
-
 D-fructose 6-phosphate pH 7.0, 55°C Clostridium thermosuccinogenes
2.7.1.90 460
-
 sedoheptulose 7-phosphate pH 7.0, 55°C Clostridium thermosuccinogenes
2.7.1.90 800
-
 sedoheptulose 7-phosphate pH 7.0, 55°C Acetivibrio thermocellus
2.7.1.90 2600
-
 D-fructose 6-phosphate pH 7.0, 55°C Clostridium thermosuccinogenes
2.7.1.90 2800
-
 D-fructose 6-phosphate pH 7.0, 55°C Acetivibrio thermocellus