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Literature summary extracted from

  • Sun, P.; Ma, T.; Zhang, T.; Zhu, H.; Zhang, J.; Liu, Y.; Ding, J.
    Molecular basis for the function of the alphabeta heterodimer of human NAD-dependent isocitrate dehydrogenase (2019), J. Biol. Chem., 294, 16214-16227 .
    View publication on PubMedView publication on EuropePMC

Activating Compound

EC Number Activating Compound Comment Organism Structure
1.1.1.41 ADP
-
 Homo sapiens
1.1.1.41 citrate
-
 Homo sapiens

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.1.1.41 expressed in Escherichia coli BL21(DE3) Codon-Plus cells Homo sapiens

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
1.1.1.41 alphabeta heterodimer with the alpha subunit in apo form and in Ca2_-bound, NAD-bound, and NADH-bound forms, hanging drop vapor diffusion method, using either containing 8% (v/v) tacsimate (pH 6.0) and 20% (w/v) PEG 3350 or 0.2 M calcium acetate (pH 7.5) and 20% (w/v) PEG 3350 Homo sapiens

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.1.1.41 NADH NADH can compete with NAD+ to bind to the active site and inhibits the activity of the alphabeta heterodimer Homo sapiens

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.1.1.41 Mg2+ activates Homo sapiens
1.1.1.41 Mn2+ 2 mM used in assay conditions Homo sapiens

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.1.1.41 isocitrate + NAD+ Homo sapiens
-
 2-oxoglutarate + CO2 + NADH + H+
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.1.1.41 Homo sapiens P50213 and O43837 subunits alpha and beta
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.1.1.41 Ni-NTA column chromatography and Superdex 200 gel filtration Homo sapiens

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.1.41 isocitrate + NAD+
-
 Homo sapiens 2-oxoglutarate + CO2 + NADH + H+
-
 ?

Subunits

EC Number Subunits Comment Organism
1.1.1.41 heterotetramer
-
 Homo sapiens

Synonyms

EC Number Synonyms Comment Organism
1.1.1.41 NAD-dependent isocitrate dehydrogenase
-
 Homo sapiens
1.1.1.41 NAD-IDH
-
 Homo sapiens

Cofactor

EC Number Cofactor Comment Organism Structure
1.1.1.41 NAD+
-
 Homo sapiens