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Literature summary extracted from

  • Avellaneda Matteo, D.; Grunseth, A.J.; Gonzalez, E.R.; Anselmo, S.L.; Kennedy, M.A.; Moman, P.; Scott, D.A.; Hoang, A.; Sohl, C.D.
    Molecular mechanisms of isocitrate dehydrogenase 1 (IDH1) mutations identified in tumors The role of size and hydrophobicity at residue 132 on catalytic efficiency (2017), J. Biol. Chem., 292, 7971-7983 .
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.1.1.42 expressed in Escherichia coli BL21 Gold (DE3) cells Homo sapiens

Protein Variants

EC Number Protein Variants Comment Organism
1.1.1.42 A134D the mutant shows severely reduced catalytic efficiency compared to the wild type enzyme Homo sapiens
1.1.1.42 H133Q the mutant shows strongly reduced catalytic efficiency compared to the wild type enzyme Homo sapiens
1.1.1.42 R100Q the mutant shows severely reduced catalytic efficiency compared to the wild type enzyme Homo sapiens
1.1.1.42 R132A the mutant shows severely reduced catalytic efficiency compared to the wild type enzyme Homo sapiens
1.1.1.42 R132C the mutant shows severely reduced catalytic efficiency compared to the wild type enzyme Homo sapiens
1.1.1.42 R132G the mutant shows severely reduced catalytic efficiency compared to the wild type enzyme Homo sapiens
1.1.1.42 R132H the mutant shows severely reduced catalytic efficiency compared to the wild type enzyme Homo sapiens
1.1.1.42 R132K the mutant shows severely reduced catalytic efficiency compared to the wild type enzyme Homo sapiens
1.1.1.42 R132N the mutant shows severely reduced catalytic efficiency compared to the wild type enzyme Homo sapiens
1.1.1.42 R132Q the mutant shows severely reduced catalytic efficiency compared to the wild type enzyme Homo sapiens
1.1.1.42 R132W the mutant shows severely reduced catalytic efficiency compared to the wild type enzyme Homo sapiens

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.1.1.42 0.0025
-
 NADPH Km less than 0.0025 mM or equal, mutant enzyme R100Q, at pH 7.5 and 21°C Homo sapiens
1.1.1.42 0.005
-
 NADPH Km less than 0.005 mM or equal, mutant enzyme R132H, at pH 7.5 and 21°C Homo sapiens
1.1.1.42 0.005
-
 NADPH mutant enzyme R100Q, at pH 7.5 and 37°C Homo sapiens
1.1.1.42 0.01
-
 NADPH Km less than 0.01 mM or equal, wild type enzyme, at pH 7.5 and 37°C Homo sapiens
1.1.1.42 0.01
-
 NADPH mutant enzyme R132C, at pH 7.5 and 37°C Homo sapiens
1.1.1.42 0.015
-
 isocitrate wild type enzyme, at pH 7.5 and 21°C Homo sapiens
1.1.1.42 0.025
-
 NADPH Km less than 0.025 mM or equal, mutant enzyme R132C, at pH 7.5 and 21°C Homo sapiens
1.1.1.42 0.025
-
 NADPH Km less than 0.025 mM or equal, mutant enzyme R132G, at pH 7.5 and 21°C Homo sapiens
1.1.1.42 0.025
-
 NADPH Km less than 0.025 mM or equal, mutant enzyme R132H, at pH 7.5 and 37°C Homo sapiens
1.1.1.42 0.025
-
 NADPH Km less than 0.025 mM, mutant enzyme R132G, at pH 7.5 and 37°C Homo sapiens
1.1.1.42 0.03
-
 NADP+ wild type enzyme, at pH 7.5 and 21°C Homo sapiens
1.1.1.42 0.067
-
 NADP+ mutant enzyme R132G, at pH 7.5 and 37°C Homo sapiens
1.1.1.42 0.07
-
 NADP+ mutant enzyme R100Q, at pH 7.5 and 21°C Homo sapiens
1.1.1.42 0.08
-
 NADP+ wild type enzyme, at pH 7.5 and 37°C Homo sapiens
1.1.1.42 0.101
-
 NADP+ mutant enzyme H133Q, at pH 7.5 and 21°C Homo sapiens
1.1.1.42 0.11
-
 2-oxoglutarate mutant enzyme R132A, at pH 7.5 and 37°C Homo sapiens
1.1.1.42 0.14
-
 NADP+ mutant enzyme R132G, at pH 7.5 and 21°C Homo sapiens
1.1.1.42 0.16
-
 NADP+ mutant enzyme H133Q, at pH 7.5 and 37°C Homo sapiens
1.1.1.42 0.18
-
 NADP+ mutant enzyme R100Q, at pH 7.5 and 37°C Homo sapiens
1.1.1.42 0.22
-
 isocitrate wild type enzyme, at pH 7.5 and 37°C Homo sapiens
1.1.1.42 0.26
-
 2-oxoglutarate mutant enzyme R132Q, at pH 7.5 and 37°C Homo sapiens
1.1.1.42 0.28
-
 isocitrate mutant enzyme H133Q, at pH 7.5 and 21°C Homo sapiens
1.1.1.42 0.3
-
 2-oxoglutarate mutant enzyme R132G, at pH 7.5 and 21°C Homo sapiens
1.1.1.42 0.34
-
 2-oxoglutarate mutant enzyme R132G, at pH 7.5 and 37°C Homo sapiens
1.1.1.42 0.36
-
 2-oxoglutarate mutant enzyme R132C, at pH 7.5 and 21°C Homo sapiens
1.1.1.42 0.36
-
 2-oxoglutarate mutant enzyme R132C, at pH 7.5 and 37°C Homo sapiens
1.1.1.42 0.4
-
 isocitrate mutant enzyme H133Q, at pH 7.5 and 37°C Homo sapiens
1.1.1.42 0.5
-
 2-oxoglutarate wild type enzyme, at pH 7.5 and 37°C Homo sapiens
1.1.1.42 0.58
-
 NADP+ mutant enzyme R132C, at pH 7.5 and 21°C Homo sapiens
1.1.1.42 0.61
-
 2-oxoglutarate mutant enzyme R132K, at pH 7.5 and 37°C Homo sapiens
1.1.1.42 0.7
-
 NADP+ mutant enzyme A134D, at pH 7.5 and 21°C Homo sapiens
1.1.1.42 0.75
-
 NADP+ mutant enzyme R132C, at pH 7.5 and 37°C Homo sapiens
1.1.1.42 0.8
-
 isocitrate mutant enzyme R132Q, at pH 7.5 and 37°C Homo sapiens
1.1.1.42 0.82
-
 2-oxoglutarate mutant enzyme R132W, at pH 7.5 and 37°C Homo sapiens
1.1.1.42 1
-
 NADP+ mutant enzyme R132H, at pH 7.5 and 21°C Homo sapiens
1.1.1.42 1.1
-
 2-oxoglutarate mutant enzyme R132H, at pH 7.5 and 37°C Homo sapiens
1.1.1.42 1.1
-
 isocitrate mutant enzyme R132K, at pH 7.5 and 37°C Homo sapiens
1.1.1.42 1.2
-
 NADP+ mutant enzyme A134D, at pH 7.5 and 37°C Homo sapiens
1.1.1.42 1.5
-
 isocitrate mutant enzyme R132N, at pH 7.5 and 37°C Homo sapiens
1.1.1.42 1.6
-
 NADP+ mutant enzyme R132H, at pH 7.5 and 37°C Homo sapiens
1.1.1.42 1.8
-
 2-oxoglutarate mutant enzyme R132H, at pH 7.5 and 21°C Homo sapiens
1.1.1.42 2.7
-
 isocitrate mutant enzyme A134D, at pH 7.5 and 21°C Homo sapiens
1.1.1.42 3.6
-
 isocitrate mutant enzyme R132G, at pH 7.5 and 21°C Homo sapiens
1.1.1.42 3.6
-
 isocitrate mutant enzyme R132W, at pH 7.5 and 37°C Homo sapiens
1.1.1.42 4.2
-
 isocitrate mutant enzyme R132H, at pH 7.5 and 37°C Homo sapiens
1.1.1.42 5.3
-
 isocitrate mutant enzyme R132C, at pH 7.5 and 21°C Homo sapiens
1.1.1.42 5.7
-
 isocitrate mutant enzyme R132A, at pH 7.5 and 37°C Homo sapiens
1.1.1.42 6
-
 isocitrate mutant enzyme R132H, at pH 7.5 and 21°C Homo sapiens
1.1.1.42 7
-
 isocitrate mutant enzyme R132G, at pH 7.5 and 37°C Homo sapiens
1.1.1.42 8
-
 isocitrate mutant enzyme A134D, at pH 7.5 and 37°C Homo sapiens
1.1.1.42 8
-
 isocitrate mutant enzyme R100Q, at pH 7.5 and 37°C Homo sapiens
1.1.1.42 8.2
-
 isocitrate mutant enzyme R132C, at pH 7.5 and 37°C Homo sapiens
1.1.1.42 9
-
 isocitrate mutant enzyme R100Q, at pH 7.5 and 21°C Homo sapiens
1.1.1.42 10
-
 2-oxoglutarate mutant enzyme R100Q, at pH 7.5 and 21°C Homo sapiens
1.1.1.42 10
-
 2-oxoglutarate mutant enzyme R132N, at pH 7.5 and 37°C Homo sapiens
1.1.1.42 12
-
 2-oxoglutarate mutant enzyme R100Q, at pH 7.5 and 37°C Homo sapiens

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
1.1.1.42 cytosol
-
 Homo sapiens 5829
-
1.1.1.42 peroxisome
-
 Homo sapiens 5777
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.1.1.42 Mg2+ 10 mM used in assay conditions Homo sapiens

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.1.1.42 isocitrate + NADP+ Homo sapiens
-
 2-oxoglutarate + CO2 + NADPH + H+
-
 r

Organism

EC Number Organism UniProt Comment Textmining
1.1.1.42 Homo sapiens O75874
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.1.1.42 Ni-NTA column chromatography Homo sapiens

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.1.42 2-oxoglutarate + NADPH + H+ the reaction is catalyzed by diverse R132 mutant enzymes Homo sapiens D-2-hydroxyglutarate + NADP+
-
 r
1.1.1.42 isocitrate + NADP+
-
 Homo sapiens 2-oxoglutarate + CO2 + NADPH + H+
-
 r

Synonyms

EC Number Synonyms Comment Organism
1.1.1.42 IDH1
-
 Homo sapiens
1.1.1.42 isocitrate dehydrogenase 1
-
 Homo sapiens

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
1.1.1.42 49.1
-
 melting temperature Homo sapiens

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.1.1.42 0.016
-
 2-oxoglutarate kcat less than 0.016 s-1 or equal, mutant enzyme H133Q, at pH 7.5 and 37°C Homo sapiens
1.1.1.42 0.017
-
 2-oxoglutarate wild type enzyme, at pH 7.5 and 21°C Homo sapiens
1.1.1.42 0.019
-
 2-oxoglutarate wild type enzyme, at pH 7.5 and 37°C Homo sapiens
1.1.1.42 0.019
-
 2-oxoglutarate kcat less than 0.019 s-1 or equal, mutant enzyme A134D, at pH 7.5 and 37°C Homo sapiens
1.1.1.42 0.02
-
 2-oxoglutarate mutant enzyme A134D, at pH 7.5 and 21°C Homo sapiens
1.1.1.42 0.034
-
 2-oxoglutarate mutant enzyme H133, at pH 7.5 and 21°C Homo sapiens
1.1.1.42 0.047
-
 isocitrate mutant enzyme R132N, at pH 7.5 and 37°C Homo sapiens
1.1.1.42 0.12
-
 isocitrate mutant enzyme R132H, at pH 7.5 and 21°C Homo sapiens
1.1.1.42 0.128
-
 2-oxoglutarate mutant enzyme R100Q, at pH 7.5 and 21°C Homo sapiens
1.1.1.42 0.2
-
 isocitrate mutant enzyme A134D, at pH 7.5 and 21°C Homo sapiens
1.1.1.42 0.34
-
 2-oxoglutarate mutant enzyme R100Q, at pH 7.5 and 37°C Homo sapiens
1.1.1.42 0.37
-
 2-oxoglutarate mutant enzyme R132A, at pH 7.5 and 37°C Homo sapiens
1.1.1.42 0.43
-
 2-oxoglutarate mutant enzyme R132H, at pH 7.5 and 21°C Homo sapiens
1.1.1.42 0.45
-
 2-oxoglutarate mutant enzyme R132G, at pH 7.5 and 21°C Homo sapiens
1.1.1.42 0.54
-
 2-oxoglutarate mutant enzyme R132W, at pH 7.5 and 37°C Homo sapiens
1.1.1.42 0.57
-
 2-oxoglutarate mutant enzyme R132K, at pH 7.5 and 37°C Homo sapiens
1.1.1.42 0.79
-
 2-oxoglutarate mutant enzyme R132N, at pH 7.5 and 37°C Homo sapiens
1.1.1.42 0.84
-
 2-oxoglutarate mutant enzyme R132C, at pH 7.5 and 21°C Homo sapiens
1.1.1.42 1
-
 isocitrate mutant enzyme R132G, at pH 7.5 and 21°C Homo sapiens
1.1.1.42 1.21
-
 isocitrate mutant enzyme R132W, at pH 7.5 and 37°C Homo sapiens
1.1.1.42 1.4
-
 isocitrate mutant enzyme R100Q, at pH 7.5 and 21°C Homo sapiens
1.1.1.42 1.59
-
 2-oxoglutarate mutant enzyme R132G, at pH 7.5 and 37°C Homo sapiens
1.1.1.42 1.6
-
 2-oxoglutarate mutant enzyme R132C, at pH 7.5 and 37°C Homo sapiens
1.1.1.42 1.61
-
 isocitrate mutant enzyme R132C, at pH 7.5 and 21°C Homo sapiens
1.1.1.42 2.3
-
 isocitrate mutant enzyme A134D, at pH 7.5 and 37°C Homo sapiens
1.1.1.42 2.4
-
 isocitrate mutant enzyme R132H, at pH 7.5 and 37°C Homo sapiens
1.1.1.42 4.2
-
 2-oxoglutarate mutant enzyme R132H, at pH 7.5 and 37°C Homo sapiens
1.1.1.42 4.4
-
 isocitrate mutant enzyme R132C, at pH 7.5 and 37°C Homo sapiens
1.1.1.42 4.7
-
 2-oxoglutarate mutant enzyme R132Q, at pH 7.5 and 37°C Homo sapiens
1.1.1.42 5.6
-
 isocitrate mutant enzyme R100Q, at pH 7.5 and 37°C Homo sapiens
1.1.1.42 7.2
-
 isocitrate mutant enzyme R132K, at pH 7.5 and 37°C Homo sapiens
1.1.1.42 9.2
-
 isocitrate mutant enzyme R132Q, at pH 7.5 and 37°C Homo sapiens
1.1.1.42 9.3
-
 isocitrate mutant enzyme R132G, at pH 7.5 and 37°C Homo sapiens
1.1.1.42 9.4
-
 isocitrate mutant enzyme H133Q, at pH 7.5 and 21°C Homo sapiens
1.1.1.42 10.4
-
 isocitrate mutant enzyme R132A, at pH 7.5 and 37°C Homo sapiens
1.1.1.42 11
-
 isocitrate wild type enzyme, at pH 7.5 and 21°C Homo sapiens
1.1.1.42 45
-
 isocitrate mutant enzyme H133Q, at pH 7.5 and 37°C Homo sapiens
1.1.1.42 85
-
 isocitrate wild type enzyme, at pH 7.5 and 37°C Homo sapiens

Cofactor

EC Number Cofactor Comment Organism Structure
1.1.1.42 NADP+
-
 Homo sapiens

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
1.1.1.42 0.013
-
 2-oxoglutarate mutant enzyme R100Q, at pH 7.5 and 21°C Homo sapiens
1.1.1.42 0.02
-
 isocitrate mutant enzyme R132H, at pH 7.5 and 21°C Homo sapiens
1.1.1.42 0.028
-
 2-oxoglutarate mutant enzyme R100Q, at pH 7.5 and 37°C Homo sapiens
1.1.1.42 0.031
-
 isocitrate mutant enzyme R132N, at pH 7.5 and 37°C Homo sapiens
1.1.1.42 0.04
-
 2-oxoglutarate wild type enzyme, at pH 7.5 and 37°C Homo sapiens
1.1.1.42 0.074
-
 isocitrate mutant enzyme A134D, at pH 7.5 and 21°C Homo sapiens
1.1.1.42 0.08
-
 2-oxoglutarate mutant enzyme R132N, at pH 7.5 and 37°C Homo sapiens
1.1.1.42 0.16
-
 isocitrate mutant enzyme R100Q, at pH 7.5 and 21°C Homo sapiens
1.1.1.42 0.24
-
 2-oxoglutarate mutant enzyme R132H, at pH 7.5 and 21°C Homo sapiens
1.1.1.42 0.28
-
 isocitrate mutant enzyme R132G, at pH 7.5 and 21°C Homo sapiens
1.1.1.42 0.29
-
 isocitrate mutant enzyme A134D, at pH 7.5 and 37°C Homo sapiens
1.1.1.42 0.3
-
 isocitrate mutant enzyme R132C, at pH 7.5 and 21°C Homo sapiens
1.1.1.42 0.34
-
 isocitrate mutant enzyme R132W, at pH 7.5 and 37°C Homo sapiens
1.1.1.42 0.54
-
 isocitrate mutant enzyme R132C, at pH 7.5 and 37°C Homo sapiens
1.1.1.42 0.57
-
 isocitrate mutant enzyme R132H, at pH 7.5 and 37°C Homo sapiens
1.1.1.42 0.659
-
 2-oxoglutarate mutant enzyme R132W, at pH 7.5 and 37°C Homo sapiens
1.1.1.42 0.7
-
 isocitrate mutant enzyme R100Q, at pH 7.5 and 37°C Homo sapiens
1.1.1.42 0.9
-
 2-oxoglutarate mutant enzyme R132K, at pH 7.5 and 37°C Homo sapiens
1.1.1.42 1.3
-
 isocitrate mutant enzyme R132G, at pH 7.5 and 37°C Homo sapiens
1.1.1.42 1.5
-
 2-oxoglutarate mutant enzyme R132G, at pH 7.5 and 21°C Homo sapiens
1.1.1.42 1.8
-
 isocitrate mutant enzyme R132A, at pH 7.5 and 37°C Homo sapiens
1.1.1.42 2.3
-
 2-oxoglutarate mutant enzyme R132C, at pH 7.5 and 21°C Homo sapiens
1.1.1.42 3.4
-
 2-oxoglutarate mutant enzyme R132A, at pH 7.5 and 37°C Homo sapiens
1.1.1.42 3.8
-
 2-oxoglutarate mutant enzyme R132H, at pH 7.5 and 37°C Homo sapiens
1.1.1.42 4.4
-
 2-oxoglutarate mutant enzyme R132C, at pH 7.5 and 37°C Homo sapiens
1.1.1.42 5
-
 2-oxoglutarate mutant enzyme R132G, at pH 7.5 and 37°C Homo sapiens
1.1.1.42 7
-
 isocitrate mutant enzyme R132K, at pH 7.5 and 37°C Homo sapiens
1.1.1.42 12
-
 isocitrate mutant enzyme R132Q, at pH 7.5 and 37°C Homo sapiens
1.1.1.42 18
-
 2-oxoglutarate mutant enzyme R132Q, at pH 7.5 and 37°C Homo sapiens
1.1.1.42 35
-
 isocitrate mutant enzyme H133Q, at pH 7.5 and 21°C Homo sapiens
1.1.1.42 110
-
 isocitrate mutant enzyme H133Q, at pH 7.5 and 37°C Homo sapiens
1.1.1.42 390
-
 isocitrate wild type enzyme, at pH 7.5 and 37°C Homo sapiens
1.1.1.42 730
-
 isocitrate wild type enzyme, at pH 7.5 and 21°C Homo sapiens