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Literature summary extracted from

  • Tavares, C.D.; Giles, D.H.; Stancu, G.; Chitjian, C.A.; Ferguson, S.B.; Wellmann, R.M.; Kaoud, T.S.; Ghose, R.; Dalby, K.N.
    Signal integration at elongation factor 2 kinase the roles of calcium, calmodulin, and Ser-500 phosphorylation (2017), J. Biol. Chem., 292, 2032-2045 .
    View publication on PubMedView publication on EuropePMC

Activating Compound

EC Number Activating Compound Comment Organism Structure
2.7.11.20 Calmodulin absolutely necessary for efficient enzyme activity Homo sapiens

Protein Variants

EC Number Protein Variants Comment Organism
2.7.11.20 D284A catalytically inactive Homo sapiens
2.7.11.20 S500D the mutation enhances the rate of activation (Thr-348 autophosphorylation) by 6fold and lowers the EC50 for Ca2+/calmpdulin binding to activated enzyme (Thr-348 phosphorylated) by 20fold as compared to the wild type Homo sapiens

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.7.11.20 Ca2+ Ca2+ enhances the affinity of calmodulin toward the enzyme, 0.15 mM Ca2+ is used in assay conditions Homo sapiens
2.7.11.20 Mg2+ 10 mM used in assay conditions Homo sapiens

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.7.11.20 ATP + [elongation factor 2] Homo sapiens
-
 ADP + [elongation factor 2] phosphate
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.7.11.20 Homo sapiens O00418
-
-

Posttranslational Modification

EC Number Posttranslational Modification Comment Organism
2.7.11.20 phosphoprotein the enzyme autophosphorylates at Thr-348. Phosphorylation of Ser-500 integrates with Ca2+ and calmodulin to regulate the enzyme activity. Phosphorylation of Thr-348 and Ser-500 regulates the Ca2+/calmodulin-dependent activity of the enzyme Homo sapiens

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.7.11.20 HEK-293T cell
-
 Homo sapiens
-
2.7.11.20 MCF-10A cell
-
 Homo sapiens
-
2.7.11.20 MDA-MB-231 cell
-
 Homo sapiens
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.11.20 ATP + [elongation factor 2]
-
 Homo sapiens ADP + [elongation factor 2] phosphate
-
 ?

Synonyms

EC Number Synonyms Comment Organism
2.7.11.20 EEF-2K
-
 Homo sapiens
2.7.11.20 eukaryotic elongation factor 2 kinase
-
 Homo sapiens