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Literature summary extracted from

  • Foulon, V.; Sniekers, M.; Huysmans, E.; Asselberghs, S.; Mahieu, V.; Mannaerts, G.P.; Van Veldhoven, P.P.; Casteels, M.
    Breakdown of 2-hydroxylated straight chain fatty acids via peroxisomal 2-hydroxyphytanoyl-CoA lyase a revised pathway for the alpha-oxidation of straight chain fatty acids (2005), J. Biol. Chem., 280, 9802-9812 .
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
4.1.2.63 expressed in Saccharomyces cerevisiae strain CB80 Rattus norvegicus

Inhibitors

EC Number Inhibitors Comment Organism Structure
4.1.2.63 additional information not inhibited in the presence of 2-keto-octanoate, 2-hydroxyhexadecanoic acid, 2-methylhexadecanoyl-CoA, 2-methylhexadecanoic acid, 3-methylhexadecanoyl-CoA, 3-hydroxy-3-methylhexadecanoyl-CoA, or 3-hydroxy-2-methylhexadecanoyl-CoA. 2-Hydroxy straight chain acyl-CoA esters compete with 2-hydroxy-3-methyl-branched acyl-CoAs for cleavage by the enzyme Rattus norvegicus

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
4.1.2.63 peroxisome
-
 Rattus norvegicus 5777
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
4.1.2.63 Mg2+ required Rattus norvegicus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
4.1.2.63 2-hydroxyphytanoyl-CoA Rattus norvegicus
-
 pristanal + formyl-CoA
-
 ?
4.1.2.63 a 2-hydroxy-3-methyl-Cn-fatty-acyl-CoA Rattus norvegicus
-
 a 2-methyl-branched Cn-1-fatty aldehyde + formyl-CoA
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
4.1.2.63 Rattus norvegicus Q8CHM7
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
4.1.2.63 Ni-NTA agarose column chromatography Rattus norvegicus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
4.1.2.63 hepatocyte
-
 Rattus norvegicus
-
4.1.2.63 liver
-
 Rattus norvegicus
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.1.2.63 2-hydroxy-3-methylhexadecanoyl-CoA
-
 Rattus norvegicus 2-hydroxy-3-methylpentadecanal + formyl-CoA
-
 ?
4.1.2.63 2-hydroxyoctadecanoyl-CoA
-
 Rattus norvegicus heptadecanal + formyl-CoA
-
 ?
4.1.2.63 2-hydroxyphytanoyl-CoA
-
 Rattus norvegicus pristanal + formyl-CoA
-
 ?
4.1.2.63 a 2-hydroxy-3-methyl-Cn-fatty-acyl-CoA
-
 Rattus norvegicus a 2-methyl-branched Cn-1-fatty aldehyde + formyl-CoA
-
 ?
4.1.2.63 additional information no activity toward 3-methylhexadecanoyl-CoA, hexadecanoyl-CoA, 3-hydroxyhexadecanoyl-CoA, octadecanoyl-CoA, 2-hydroxy-3-methylhexadecanoic acid, 3-methylhexadecanoic acid, hexadecanoic acid, and 2-hydroxyhexadecanoic acid. The addition of 0.05 mM 2-hydroxyhexadecanoyl-CoA or 2-hydroxyoctadecanoyl-CoA to incubations with 0.01 mM 2-hydroxy-3-methylhexadecanoyl-CoA reduces the cleavage rates to a major extent Rattus norvegicus ?
-
-

Subunits

EC Number Subunits Comment Organism
4.1.2.63 ? x * 63000, SDS-PAGE Rattus norvegicus

Synonyms

EC Number Synonyms Comment Organism
4.1.2.63 2-HPCL
-
 Rattus norvegicus
4.1.2.63 2-hydroxyphytanoyl-CoA lyase
-
 Rattus norvegicus

Cofactor

EC Number Cofactor Comment Organism Structure
4.1.2.63 ATP dependent on. No oxidation is detected upon the addition of GTP or AMP Rattus norvegicus
4.1.2.63 CoA dependent on. CoA cannot be replaced by desulfo-CoA or dephospho-CoA Rattus norvegicus
4.1.2.63 NAD+ dependent on. Extra addition of NAD+ results in a 20% increase of total oxidation Rattus norvegicus
4.1.2.63 thiamine diphosphate dependent on Rattus norvegicus

General Information

EC Number General Information Comment Organism
4.1.2.63 metabolism essential enzyme in the peroxisomal alpha-oxidation of 3-methyl-branched fatty acids such as phytanic acid Rattus norvegicus