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Literature summary extracted from

  • Kahnert, A.; Kertesz, M.
    Characterization of a sulfur-regulated oxygenative alkylsulfatase from Pseudomonas putida S-313 (2000), J. Biol. Chem., 275, 31661-31667 .
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
1.14.11.77 ascorbate 0.2 mM, 3fold increase in activity Pseudomonas putida

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.14.11.77 expression in Escherichia coli Pseudomonas putida

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.14.11.77 EDTA 0.1 mM, complete loss of activity Pseudomonas putida

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.14.11.77 0.034
-
 Dodecyl sulfate pH 7, 30°C Pseudomonas putida
1.14.11.77 0.04
-
 hexyl sulfate pH 7, 30°C Pseudomonas putida
1.14.11.77 0.06
-
 nonyl sulfate pH 7, 30°C Pseudomonas putida
1.14.11.77 0.066
-
 2-ethylhexyl sulfate pH 7, 30°C Pseudomonas putida
1.14.11.77 0.097
-
 decyl sulfate pH 7, 30°C Pseudomonas putida
1.14.11.77 0.14
-
 2-oxoglutarate pH 7, 30°C Pseudomonas putida
1.14.11.77 0.271
-
 heptyl sulfate pH 7, 30°C Pseudomonas putida
1.14.11.77 0.437
-
 octyl sulfate pH 7, 30°C Pseudomonas putida

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.14.11.77 Fe2+ required, maximum acitivy at 0.1 mM Pseudomonas putida
1.14.11.77 additional information not activating: Ni2+, Co2+, Mn2+, Cu2+, Zn2+, Mg2+, and Ca2+ Pseudomonas putida

Organism

EC Number Organism UniProt Comment Textmining
1.14.11.77 Pseudomonas putida Q9WWU5
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.14.11.77 recombinant protein Pseudomonas putida

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.14.11.77 2-ethylhexyl sulfate + 2-oxoglutarate + O2
-
 Pseudomonas putida 2-ethylhexanal + succinate + CO2 + sulfate
-
 ?
1.14.11.77 decyl sulfate + 2-oxoglutarate + O2
-
 Pseudomonas putida decanal + succinate + CO2 + sulfate
-
 ?
1.14.11.77 dodecyl sulfate + 2-oxoglutarate + O2
-
 Pseudomonas putida dodecanal + succinate + CO2 + sulfate
-
 ?
1.14.11.77 heptyl sulfate + 2-oxoglutarate + O2
-
 Pseudomonas putida heptanal + succinate + CO2 + sulfate
-
 ?
1.14.11.77 hexyl sulfate + 2-oxoglutarate + O2
-
 Pseudomonas putida hexanal + succinate + CO2 + sulfate
-
 ?
1.14.11.77 additional information 2-keto acids support desulfation at the following rates, relative to the rate obtained with 2-oxoglutarate: 2-oxo-valerate 87%, 2-oxo-adipate 81%, 2-oxo-octanoate 31%, 3-methyl-2-oxo-butyrate 25%, and oxaloacetate 15%. No desulfation is obtained with pyruvate Pseudomonas putida ?
-
-
1.14.11.77 nonyl sulfate + 2-oxoglutarate + O2
-
 Pseudomonas putida nonanal + succinate + CO2 + sulfate
-
 ?
1.14.11.77 octyl sulfate + 2-oxoglutarate + O2
-
 Pseudomonas putida octanal + succinate + CO2 + sulfate
-
 ?

Subunits

EC Number Subunits Comment Organism
1.14.11.77 tetramer 4 * 32000, SDS-PAGE Pseudomonas putida

Synonyms

EC Number Synonyms Comment Organism
1.14.11.77 alpha-ketoglutarate-dependent sulfate ester dioxygenase
-
 Pseudomonas putida
1.14.11.77 AtsK
-
 Pseudomonas putida

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.14.11.77 6.5 7.5
-
 Pseudomonas putida

General Information

EC Number General Information Comment Organism
1.14.11.77 metabolism AtsK protein catalyzes the alpha-ketoglutarate-dependent cleavage of a range of alkyl sulfate esters, with chain lengths ranging from C4 to C12, requires oxygen and Fe2+ for activity and releases succinate, sulfate, and the corresponding aldehyde as products Pseudomonas putida