EC Number | Activating Compound | Comment | Organism | Structure |
---|---|---|---|---|
1.5.1.54 | S-adenosyl-L-methionine | stimulation by 10-20% | Arabidopsis thaliana | |
1.5.1.54 | S-adenosyl-L-methionine | stimulation by 50-70% | Arabidopsis thaliana |
EC Number | General Stability | Organism |
---|---|---|
1.5.1.54 | unstable, loss of 50% of activity during 30 min on ice | Arabidopsis thaliana |
EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
1.5.1.54 | additional information | not inhibitory: S-Methyl-L-methionine; not inhibitory: S-Methyl-L-methionine | Arabidopsis thaliana | |
1.5.1.54 | additional information | not inhibitory: S-adenosyl-L-methionine and S-Methyl-L-methionine | Zea mays |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
1.5.1.54 | Arabidopsis thaliana | O80585 | isoform MTHFR2, cf. EC 1.5.1.20 | - |
1.5.1.54 | Arabidopsis thaliana | Q9SE60 | isoform MTHFR1, cf. EC 1.5.1.20 | - |
1.5.1.54 | Zea mays | Q9SE94 | cf. EC 1.5.1.20 | - |
EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|---|
1.5.1.54 | 6.9 | - |
pH 7.2, 30°C | Arabidopsis thaliana |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.5.1.54 | 5,10-methylenetetrahydrofolate + menadiol | - |
Zea mays | 5-methyltetrahydrofolate + menadione | - |
r | |
1.5.1.54 | 5,10-methylenetetrahydrofolate + menadiol | - |
Arabidopsis thaliana | 5-methyltetrahydrofolate + menadione | - |
r | |
1.5.1.54 | 5,10-methylenetetrahydrofolate + NADH + H+ | - |
Zea mays | 5-methyltetrahydrofolate + NAD+ | - |
? | |
1.5.1.54 | 5,10-methylenetetrahydrofolate + NADH + H+ | - |
Arabidopsis thaliana | 5-methyltetrahydrofolate + NAD+ | - |
? | |
1.5.1.54 | 5-methyltetrahydrofolate + menadione | - |
Zea mays | 5,10-methylenetetrahydrofolate + menadiol | - |
r | |
1.5.1.54 | 5-methyltetrahydrofolate + menadione | - |
Arabidopsis thaliana | 5,10-methylenetetrahydrofolate + menadiol | - |
r |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
1.5.1.54 | ? | x * 66300, calculated from sequence and SDS-PAGE | Arabidopsis thaliana |
1.5.1.54 | ? | x * 66800, calculated from sequence | Arabidopsis thaliana |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
1.5.1.54 | MTHFR1 | - |
Arabidopsis thaliana |
1.5.1.54 | MTHFR2 | - |
Arabidopsis thaliana |
EC Number | Cofactor | Comment | Organism | Structure |
---|---|---|---|---|
1.5.1.54 | NADH | enzyme strongly prefers NADH over NADPH | Zea mays | |
1.5.1.54 | NADH | enzyme strongly prefers NADH over NADPH | Arabidopsis thaliana |
EC Number | General Information | Comment | Organism |
---|---|---|---|
1.5.1.54 | physiological function | enzyme is able to complement a Saccharomyces cerevisiae met12 met13 double disruptant | Zea mays |
1.5.1.54 | physiological function | enzyme is able to complement a Saccharomyces cerevisiae met12 met13 double disruptant | Arabidopsis thaliana |