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Literature summary extracted from
- Purification and properties of a phosphatidic acid-preferring phospholipase A1 from bovine testis. Examination of the molecular basis of its activation (1996), J. Biol. Chem., 271, 10874-10883 .
Activating Compound
| EC Number | Activating Compound | Comment | Organism | Structure |
|---|---|---|---|---|
| 3.1.1.118 | additional information | a nonhydrolyzable sn-1-alkyl-2-oleoyl analogue of phosphatidic acid activates the enzyme 30fold | Bos taurus |
Inhibitors
| EC Number | Inhibitors | Comment | Organism | Structure |
|---|---|---|---|---|
| 3.1.1.118 | methyl arachidonyl fluorophosphonate | - |
Bos taurus |  |
Localization
| EC Number | Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 3.1.1.118 | Bos taurus | O46606 | - |
- |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 3.1.1.118 | ammonium sulfate precipitation, SP-Sepharose column chromatography, Mono Q column chromatography, Phenyl-Superose column chromatography, and Superdex 200 gel filtration | Bos taurus |
Source Tissue
| EC Number | Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|---|
| 3.1.1.118 | testis | - |
Bos taurus | - |
Specific Activity [micromol/min/mg]
| EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|---|
| 3.1.1.118 | 0.00021 | - |
supernatant, with 1,2-dioleoyl-sn-phosphatidic acid as substrate, at pH 8.0 and 25°C | Bos taurus |
| 3.1.1.118 | 2.95 | - |
after 14048fold purification, with 1,2-dioleoyl-sn-phosphatidic acid as substrate, at pH 8.0 and 25°C | Bos taurus |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 3.1.1.118 | 1,2-diarachidonoyl-sn-phosphatidic acid + H2O | 246% activity compared to 1,2-dioleoyl-sn-phosphatidic acid | Bos taurus | 2-arachidonoyl-sn-phosphatidic acid + arachidonoate | - |
? | |
| 3.1.1.118 | 1,2-dielaidoyl-sn-phosphatidic acid + H2O | 40% activity compared to 1,2-dioleoyl-sn-phosphatidic acid | Bos taurus | 2-elaidoyl-sn-phosphatidic acid + elaidate | - |
? | |
| 3.1.1.118 | 1,2-dilinolenoyl-sn-phosphatidic acid + H2O | 235% activity compared to 1,2-dioleoyl-sn-phosphatidic acid | Bos taurus | 2-linolenoyl-sn-phosphatidic acid + linolenate | - |
? | |
| 3.1.1.118 | 1,2-dilinoleoyl-sn-phosphatidic acid + H2O | 214% activity compared to 1,2-dioleoyl-sn-phosphatidic acid | Bos taurus | 2-linoleoyl-sn-phosphatidic acid + linoleate | - |
? | |
| 3.1.1.118 | 1,2-dioleoyl-sn-phosphatidic acid + H2O | 100% activity | Bos taurus | 2-oleoyl-sn-phosphatidic acid + oleate | - |
? | |
| 3.1.1.118 | 1,2-dioleoyl-sn-phosphatidylcholine + H2O | 5% activity compared to 1-dioleoyl-sn-phosphatidic acid | Bos taurus | 2-oleoyl-sn-phosphatidylcholine + oleate | - |
? | |
| 3.1.1.118 | 1,2-dioleoyl-sn-phosphatidylethanolamine + H2O | 11% activity compared to 1-dioleoyl-sn-phosphatidic acid | Bos taurus | 2-oleoyl-sn-phosphatidylethanolamine + oleate | - |
? | |
| 3.1.1.118 | 1,2-dioleoyl-sn-phosphatidylinositol + H2O | 14% activity compared to 1-dioleoyl-sn-phosphatidic acid | Bos taurus | 2-oleoyl-sn-phosphatidylinositol + oleate | - |
? | |
| 3.1.1.118 | 1,2-dipetroselinoyl-sn-phosphatidic acid + H2O | 21% activity compared to 1-dioleoyl-sn-phosphatidic acid | Bos taurus | 2-petroselinoyl-sn-phosphatidic acid + petroselinate | - |
? | |
| 3.1.1.118 | 1-palmitoyl-2-arachidonoyl-sn-phosphatdidylcholine + H2O | - |
Bos taurus | 2-arachidonoyl-sn-phosphatidylcholine + palmitate | - |
? | |
| 3.1.1.118 | 1-palmitoyl-2-arachidonoyl-sn-phosphatdidylethanolamine + H2O | - |
Bos taurus | 2-arachidonoyl-sn-phosphatidylethanolamine + palmitate | - |
? | |
| 3.1.1.118 | 1-palmitoyl-2-oleoyl-sn-phosphatidic acid + H2O | 24% activity compared to 1-dioleoyl-sn-phosphatidic acid | Bos taurus | 2-oleoyl-sn-phosphatidic acid + palmitate | - |
? | |
| 3.1.1.118 | 1-stearoyl-2-arachidonoyl-sn-phosphatidic acid + H2O | 31% activity compared to 1-dioleoyl-sn-phosphatidic acid | Bos taurus | 2-arachidonoyl-sn-phosphatidic acid + stearate | - |
? | |
Subunits
| EC Number | Subunits | Comment | Organism |
|---|---|---|---|
| 3.1.1.118 | homotetramer | 4 * 110000, SDS-PAGE | Bos taurus |
Synonyms
| EC Number | Synonyms | Comment | Organism |
|---|---|---|---|
| 3.1.1.118 | PA-PLA1 | - |
Bos taurus |
| 3.1.1.118 | phosphatidic acid-preferring phospholipase A1 | - |
Bos taurus |
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